Click here to switch to the map view.

The map label for this gene is pcrA [H]

Identifier: 170077156

GI number: 170077156

Start: 561404

End: 563770

Strand: Direct

Name: pcrA [H]

Synonym: SYNPCC7002_A0531

Alternate gene names: 170077156

Gene position: 561404-563770 (Clockwise)

Preceding gene: 170077155

Following gene: 170077157

Centisome position: 18.66

GC content: 50.4

Gene sequence:

>2367_bases
ATGTCTGACTTGTTAGCCCAACTCAACCCCTCCCAACGTCGTGCTGTTGAACATTTTTGTGGGCCACTCCTGGTGGTGGC
CGGGGCTGGCTCAGGCAAAACCAGGGCCTTGACGTTTCGCATTGCCCATTTGATTCGCAACCACCGTGTTGATCCAGAGA
ATATTCTGGCCGTCACCTTTACGAACAAGGCTGCCCGCGAAATGAAAGAACGGGTAGAACTGCTCTATGCCCAACAGCTC
GCAGAACTGCAACATGGTAAACCCCTGGGAGGGCTGCCGGAGTTTGAGCAAAAAAAATTGCGATCGCAGGTTTACAAGCA
GGTGACAAAACAGCTCTGGATTGGCACGTTTCACAGCCTCTGCGCGCGAATTCTCCGGTACGACATCAACAAATATCAGG
ACGAACGGGGCCGCACCTGGCAGCGCAATTTCACGATCATGGACGATAGCGACGTCCAAACATTGATCAAACGCATTGTC
ACCCAGCAACTGAACCTAGACGATAAAAAATTCAATCCCCGCACCATCCGCTATCAGATTAGTAATGCCAAAAACCTCGG
CCTTACCCCCAATGACTATGCCCGGCAGCAGGGAGGTTACAAAGGGAAAGTTGTCGCGGAGGTTTATGAAGCCTATCAAA
CGGCCTTGGCGGCGAATAATTCCCTCGATTTTGATGATTTAATTCTGATCCCAGTGCGGTTGTTCCAGCAAAATGAATCG
ATCCTCGGTTATTGGCACAGTCAATTTCACCATATTCTTGTGGATGAGTATCAGGATACAAACCGGATTCAGTATGAGTT
AATTCGGCTACTCAGTACCAATGGCGAAACAAACCAGGCCGCTTTGGGTTGGCATAATCGCTCTTTGTTTGTGGTAGGGG
ATGCGGATCAATCCATTTATTCGTTTCGGATGGCGGACTTCACAATCTTGCTGGAATTCCAAGAAAATTTTGGCGATCGC
CTCCCCGATGACGAAACCCAGACGATGGTTAAGTTGGAGGAAAATTACCGCTCCCGGGAAAATATTCTGAAAGCCGCCAA
TGCCTTGATCGAGCAAAATAGCCAGCGCATCGATAAAGTGCTCAAGGCCACCCGGGGCGCTGGAGAGGAAATTTATTGCC
ACAAGGCGGATAACGAAAACGAAGAAGCGCGATTCGTTTTAAATCAAATCCTCGGTCTGCGGCGCAAAAATCCAGAACTC
GACTGGGGCGACTTTGCGATCCTCTACCGCACCAATGCCCAATCCCGCCCCTTTGAAGATCTCCTGATTCGTAACAATAT
TCCCTACCAAGTGGTGGGCGGCTTCAAATTCTATGACCGCAAAGAGATTAAAGATGCCCTCGCTTACCTCAAGGCGATCG
CCAACCCTGCCGATACCCTGAGCCTGATGCGGGTGATTAATACTCCTAAGCGGGGCATCGGCAAAACCACCGTCGATAAA
CTTAACCACGCCGCCCAAGAATTAGGCTGTCCTCTCTGGGAAATCCTCAGCGATGAAACCTCTGTAGCCACCATCGCTGG
ACGGGCGGCCCGGAAAATCAATGAGTTTGCTGGGCTAATCCAGGAAATGCAAGGCAAAATGGCGACCATGCGCGGTGCCG
ATATCCTCGATTATGTGATGGAAAATTCCGGCTATATCGATGATTTAAAAATGCAAGGTACTGAAGAAGCCGATAGTCGT
GTGGAAAATATTTCCGAGCTTTACAATGCAATGCTGCAATTCCAAGACGAAAACGAAGACAGCAGCTTAAATGGCTTTCT
GGAAAATGCGTCCCTCGCTTCGGATCTCGATGACCTCAATGATGAGTCCCAGCAGGTTTCTTTGATGACGCTCCATTCAG
CCAAGGGTTTGGAGTTTCCGGTGGTCTTTTTGGTAGGGCTAGAGCAGGGGCTATTTCCCCATACCCGCTCCTTAAATGAT
CCCGTTGCTTTAGAAGAAGAACGGCGGTTGTGTTACGTGGGCCTGACCCGCGCCCAGGAGCAATTATTCCTCAGCTATAC
CAGGGAGCGGTATATGTGGGGTTATCGAGAGCCTGCCGTTGCCTCGCAATTTCTCGCAGAACTCCCCCAGGAGTTGGTAA
CTTCCAATGGGCGATCGCCAGCGCCAATGCCCCCGAAACAGGAACCAAAGATGATGTCCATTAGCCAGAAGCAACGCTCC
CAGAAAAGACGGGAACGACAGGCCGCCCGCACCGAAGTGGCCCAACAAAAATGGGACATTGGCGATCGCGTCCACCACAA
CGTCTTTGGGGAAGGGGAAGTGATTAAAATTTTTGGGGATGACAAAAAGACCAACCTGGCGATCCAGTTTCCCAATCTCG
GTAAAAAAATCATTGATCCCCGGGTGGCCCCGATGAAAAAGCTTTAA

Upstream 100 bases:

>100_bases
TCTACAAAGGGGCGATCGCCGAGATGAACTAACCGAACCCTGCCACGCCGGGGAGTATGTCAAAATAAGGTCTCTCCTTG
TGCTGTGAACCTTACCTTCC

Downstream 100 bases:

>100_bases
GTAGATACCAAATTAAAAAAGATTATGATTGCCCTCAGTGACCATTCATACCTAACTCCAGAAGAATATCTCGCCTTTGA
AGCCGCCAGTGACATTCGTC

Product: ATP-dependent DNA helicase II PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 788; Mature: 787

Protein sequence:

>788_residues
MSDLLAQLNPSQRRAVEHFCGPLLVVAGAGSGKTRALTFRIAHLIRNHRVDPENILAVTFTNKAAREMKERVELLYAQQL
AELQHGKPLGGLPEFEQKKLRSQVYKQVTKQLWIGTFHSLCARILRYDINKYQDERGRTWQRNFTIMDDSDVQTLIKRIV
TQQLNLDDKKFNPRTIRYQISNAKNLGLTPNDYARQQGGYKGKVVAEVYEAYQTALAANNSLDFDDLILIPVRLFQQNES
ILGYWHSQFHHILVDEYQDTNRIQYELIRLLSTNGETNQAALGWHNRSLFVVGDADQSIYSFRMADFTILLEFQENFGDR
LPDDETQTMVKLEENYRSRENILKAANALIEQNSQRIDKVLKATRGAGEEIYCHKADNENEEARFVLNQILGLRRKNPEL
DWGDFAILYRTNAQSRPFEDLLIRNNIPYQVVGGFKFYDRKEIKDALAYLKAIANPADTLSLMRVINTPKRGIGKTTVDK
LNHAAQELGCPLWEILSDETSVATIAGRAARKINEFAGLIQEMQGKMATMRGADILDYVMENSGYIDDLKMQGTEEADSR
VENISELYNAMLQFQDENEDSSLNGFLENASLASDLDDLNDESQQVSLMTLHSAKGLEFPVVFLVGLEQGLFPHTRSLND
PVALEEERRLCYVGLTRAQEQLFLSYTRERYMWGYREPAVASQFLAELPQELVTSNGRSPAPMPPKQEPKMMSISQKQRS
QKRRERQAARTEVAQQKWDIGDRVHHNVFGEGEVIKIFGDDKKTNLAIQFPNLGKKIIDPRVAPMKKL

Sequences:

>Translated_788_residues
MSDLLAQLNPSQRRAVEHFCGPLLVVAGAGSGKTRALTFRIAHLIRNHRVDPENILAVTFTNKAAREMKERVELLYAQQL
AELQHGKPLGGLPEFEQKKLRSQVYKQVTKQLWIGTFHSLCARILRYDINKYQDERGRTWQRNFTIMDDSDVQTLIKRIV
TQQLNLDDKKFNPRTIRYQISNAKNLGLTPNDYARQQGGYKGKVVAEVYEAYQTALAANNSLDFDDLILIPVRLFQQNES
ILGYWHSQFHHILVDEYQDTNRIQYELIRLLSTNGETNQAALGWHNRSLFVVGDADQSIYSFRMADFTILLEFQENFGDR
LPDDETQTMVKLEENYRSRENILKAANALIEQNSQRIDKVLKATRGAGEEIYCHKADNENEEARFVLNQILGLRRKNPEL
DWGDFAILYRTNAQSRPFEDLLIRNNIPYQVVGGFKFYDRKEIKDALAYLKAIANPADTLSLMRVINTPKRGIGKTTVDK
LNHAAQELGCPLWEILSDETSVATIAGRAARKINEFAGLIQEMQGKMATMRGADILDYVMENSGYIDDLKMQGTEEADSR
VENISELYNAMLQFQDENEDSSLNGFLENASLASDLDDLNDESQQVSLMTLHSAKGLEFPVVFLVGLEQGLFPHTRSLND
PVALEEERRLCYVGLTRAQEQLFLSYTRERYMWGYREPAVASQFLAELPQELVTSNGRSPAPMPPKQEPKMMSISQKQRS
QKRRERQAARTEVAQQKWDIGDRVHHNVFGEGEVIKIFGDDKKTNLAIQFPNLGKKIIDPRVAPMKKL
>Mature_787_residues
SDLLAQLNPSQRRAVEHFCGPLLVVAGAGSGKTRALTFRIAHLIRNHRVDPENILAVTFTNKAAREMKERVELLYAQQLA
ELQHGKPLGGLPEFEQKKLRSQVYKQVTKQLWIGTFHSLCARILRYDINKYQDERGRTWQRNFTIMDDSDVQTLIKRIVT
QQLNLDDKKFNPRTIRYQISNAKNLGLTPNDYARQQGGYKGKVVAEVYEAYQTALAANNSLDFDDLILIPVRLFQQNESI
LGYWHSQFHHILVDEYQDTNRIQYELIRLLSTNGETNQAALGWHNRSLFVVGDADQSIYSFRMADFTILLEFQENFGDRL
PDDETQTMVKLEENYRSRENILKAANALIEQNSQRIDKVLKATRGAGEEIYCHKADNENEEARFVLNQILGLRRKNPELD
WGDFAILYRTNAQSRPFEDLLIRNNIPYQVVGGFKFYDRKEIKDALAYLKAIANPADTLSLMRVINTPKRGIGKTTVDKL
NHAAQELGCPLWEILSDETSVATIAGRAARKINEFAGLIQEMQGKMATMRGADILDYVMENSGYIDDLKMQGTEEADSRV
ENISELYNAMLQFQDENEDSSLNGFLENASLASDLDDLNDESQQVSLMTLHSAKGLEFPVVFLVGLEQGLFPHTRSLNDP
VALEEERRLCYVGLTRAQEQLFLSYTRERYMWGYREPAVASQFLAELPQELVTSNGRSPAPMPPKQEPKMMSISQKQRSQ
KRRERQAARTEVAQQKWDIGDRVHHNVFGEGEVIKIFGDDKKTNLAIQFPNLGKKIIDPRVAPMKKL

Specific function: DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substra

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=787, Percent_Identity=37.7382465057179, Blast_Score=450, Evalue=1e-127,
Organism=Escherichia coli, GI48994965, Length=700, Percent_Identity=38.1428571428571, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1787196, Length=369, Percent_Identity=29.2682926829268, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322369, Length=857, Percent_Identity=28.9381563593932, Blast_Score=255, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6324477, Length=731, Percent_Identity=23.5294117647059, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 90134; Mature: 90003

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLLAQLNPSQRRAVEHFCGPLLVVAGAGSGKTRALTFRIAHLIRNHRVDPENILAVTF
CCCHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHEEEEEE
TNKAAREMKERVELLYAQQLAELQHGKPLGGLPEFEQKKLRSQVYKQVTKQLWIGTFHSL
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
CARILRYDINKYQDERGRTWQRNFTIMDDSDVQTLIKRIVTQQLNLDDKKFNPRTIRYQI
HHHHHHHHHHHHHHHCCCEEECCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
SNAKNLGLTPNDYARQQGGYKGKVVAEVYEAYQTALAANNSLDFDDLILIPVRLFQQNES
CCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCH
ILGYWHSQFHHILVDEYQDTNRIQYELIRLLSTNGETNQAALGWHNRSLFVVGDADQSIY
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCHHH
SFRMADFTILLEFQENFGDRLPDDETQTMVKLEENYRSRENILKAANALIEQNSQRIDKV
HHHHHCEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LKATRGAGEEIYCHKADNENEEARFVLNQILGLRRKNPELDWGDFAILYRTNAQSRPFED
HHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHH
LLIRNNIPYQVVGGFKFYDRKEIKDALAYLKAIANPADTLSLMRVINTPKRGIGKTTVDK
HHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHH
LNHAAQELGCPLWEILSDETSVATIAGRAARKINEFAGLIQEMQGKMATMRGADILDYVM
HHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ENSGYIDDLKMQGTEEADSRVENISELYNAMLQFQDENEDSSLNGFLENASLASDLDDLN
HCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHCC
DESQQVSLMTLHSAKGLEFPVVFLVGLEQGLFPHTRSLNDPVALEEERRLCYVGLTRAQE
CCHHEEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHH
QLFLSYTRERYMWGYREPAVASQFLAELPQELVTSNGRSPAPMPPKQEPKMMSISQKQRS
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
QKRRERQAARTEVAQQKWDIGDRVHHNVFGEGEVIKIFGDDKKTNLAIQFPNLGKKIIDP
HHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEECCCCCCEEEEECCCCCHHHCCC
RVAPMKKL
CCCCCCCC
>Mature Secondary Structure 
SDLLAQLNPSQRRAVEHFCGPLLVVAGAGSGKTRALTFRIAHLIRNHRVDPENILAVTF
CCHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHEEEEEE
TNKAAREMKERVELLYAQQLAELQHGKPLGGLPEFEQKKLRSQVYKQVTKQLWIGTFHSL
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
CARILRYDINKYQDERGRTWQRNFTIMDDSDVQTLIKRIVTQQLNLDDKKFNPRTIRYQI
HHHHHHHHHHHHHHHCCCEEECCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
SNAKNLGLTPNDYARQQGGYKGKVVAEVYEAYQTALAANNSLDFDDLILIPVRLFQQNES
CCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCH
ILGYWHSQFHHILVDEYQDTNRIQYELIRLLSTNGETNQAALGWHNRSLFVVGDADQSIY
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCHHH
SFRMADFTILLEFQENFGDRLPDDETQTMVKLEENYRSRENILKAANALIEQNSQRIDKV
HHHHHCEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LKATRGAGEEIYCHKADNENEEARFVLNQILGLRRKNPELDWGDFAILYRTNAQSRPFED
HHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHH
LLIRNNIPYQVVGGFKFYDRKEIKDALAYLKAIANPADTLSLMRVINTPKRGIGKTTVDK
HHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHH
LNHAAQELGCPLWEILSDETSVATIAGRAARKINEFAGLIQEMQGKMATMRGADILDYVM
HHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ENSGYIDDLKMQGTEEADSRVENISELYNAMLQFQDENEDSSLNGFLENASLASDLDDLN
HCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHCC
DESQQVSLMTLHSAKGLEFPVVFLVGLEQGLFPHTRSLNDPVALEEERRLCYVGLTRAQE
CCHHEEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHH
QLFLSYTRERYMWGYREPAVASQFLAELPQELVTSNGRSPAPMPPKQEPKMMSISQKQRS
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
QKRRERQAARTEVAQQKWDIGDRVHHNVFGEGEVIKIFGDDKKTNLAIQFPNLGKKIIDP
HHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEECCCCCCEEEEECCCCCHHHCCC
RVAPMKKL
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9592155; 8934527; 10199404; 10388562; 10454638 [H]