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The map label for this gene is bioB

Identifier: 170076937

GI number: 170076937

Start: 323959

End: 325062

Strand: Direct

Name: bioB

Synonym: SYNPCC7002_A0309

Alternate gene names: 170076937

Gene position: 323959-325062 (Clockwise)

Preceding gene: 170076928

Following gene: 170076938

Centisome position: 10.77

GC content: 55.43

Gene sequence:

>1104_bases
GTGGTTCAAGTACCGATCAAGCCCACCCCGCAAACCCCCGCCCCCACCACTGCAATTCCCAATGGCCCGGCCCTCGAAGC
TTGGTTAAATGACTGGGCGCAGCGGATTATTAATGGCGATCGCCTCACCCGTACCGAAGCTCTCCGTCTCACCGCCATCA
CCGGCCCCGAAAATATCCTCAAACTCTGTGCCGCTGCGGATCTGATCCGGCATGAATGCTGTGGTAATACCGTTGACCTC
TGCAGCATTGTGAATGTGAAATCTGGCAGTTGCTCCGAAAACTGTAGTTTTTGCTCCCAGTCCGCCCACCACCCCGGCGA
AGATTCCCCTGTGTATGGCCTCAAAACCGCCGAAGAAATTATTGAACAGGCCAAAGCCGCTGAAGCCGCTGGGGCAAAGC
GTTTTTGTCTCGTTAGCCAAGGCCGGGGCATTAAGTACAACAGCCCCAGGGATACTGAATTTGAGCAAATTTTAGAAACC
GTGCGCCGCATCCAGGCAGAAACAGCGATCAAACCCTGCTGTGCCCTCGGCGAAGTCACCCCAGAACAAGCCCAACAGCT
CCGGGAAGCCGGCGTCACCCGCTACAACCACAACCTCGAAGCTTCGGAAAATTTCTTCCCCGATATCGTCACCACCCACA
GTTGGCAAGACCGGGTCGAAACCATCAAAAATCTCAAAGCAGCGGGCATTCAGGCGTGCAGTGGTGGCATTATGGGCCTC
GGTGAAAGTTGGGAAGACCGGGTTGATTTGGCGATCGCCCTGCGGGATCTCGAAGTCGAATCTGTGCCTTTGAATCTCCT
CAATCCCCGTCAGGGAACTCCCCTCGGAAGCAATGACCGTCTCGATGTCTACGAAGCCCTCAAATGCATGGCAATCTTCC
GGTTTATCTTGCCCGAACAAATTATCCGCTATGCTGGCGGTCGCGAAGCGGTAATGGGTGAACTACAACACCTCGGCCTG
AAGGCAGGCATTAACGCGATGCTCATCGGCCACTACCTCACCACCATGGGCCAACCCCCCGAACAGGATCAAGCGATGCT
CAAATCCCTCGGCTTACAAGGGGGTGAAGCACCGATTCCTGGCCAATATTCTGCCGCCCAATAA

Upstream 100 bases:

>100_bases
TGCCCCAAGATGTAACAGATGTACAGAGAACCTTTAAGCCAGGGAAACGGGAATGATAAAATTTGGAATACTTTTTTAGT
AAAAGAGAGAGAAGGTCAAC

Downstream 100 bases:

>100_bases
CCCTTGAAAGCGAAAGCCCTCCCCAGATCCTTGTCCCCAAAGCAAACTGCCACCACTGGCCCGAACTGGTTTAACCAACT
CCTGTGGGCATTGATCGGTG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MVQVPIKPTPQTPAPTTAIPNGPALEAWLNDWAQRIINGDRLTRTEALRLTAITGPENILKLCAAADLIRHECCGNTVDL
CSIVNVKSGSCSENCSFCSQSAHHPGEDSPVYGLKTAEEIIEQAKAAEAAGAKRFCLVSQGRGIKYNSPRDTEFEQILET
VRRIQAETAIKPCCALGEVTPEQAQQLREAGVTRYNHNLEASENFFPDIVTTHSWQDRVETIKNLKAAGIQACSGGIMGL
GESWEDRVDLAIALRDLEVESVPLNLLNPRQGTPLGSNDRLDVYEALKCMAIFRFILPEQIIRYAGGREAVMGELQHLGL
KAGINAMLIGHYLTTMGQPPEQDQAMLKSLGLQGGEAPIPGQYSAAQ

Sequences:

>Translated_367_residues
MVQVPIKPTPQTPAPTTAIPNGPALEAWLNDWAQRIINGDRLTRTEALRLTAITGPENILKLCAAADLIRHECCGNTVDL
CSIVNVKSGSCSENCSFCSQSAHHPGEDSPVYGLKTAEEIIEQAKAAEAAGAKRFCLVSQGRGIKYNSPRDTEFEQILET
VRRIQAETAIKPCCALGEVTPEQAQQLREAGVTRYNHNLEASENFFPDIVTTHSWQDRVETIKNLKAAGIQACSGGIMGL
GESWEDRVDLAIALRDLEVESVPLNLLNPRQGTPLGSNDRLDVYEALKCMAIFRFILPEQIIRYAGGREAVMGELQHLGL
KAGINAMLIGHYLTTMGQPPEQDQAMLKSLGLQGGEAPIPGQYSAAQ
>Mature_367_residues
MVQVPIKPTPQTPAPTTAIPNGPALEAWLNDWAQRIINGDRLTRTEALRLTAITGPENILKLCAAADLIRHECCGNTVDL
CSIVNVKSGSCSENCSFCSQSAHHPGEDSPVYGLKTAEEIIEQAKAAEAAGAKRFCLVSQGRGIKYNSPRDTEFEQILET
VRRIQAETAIKPCCALGEVTPEQAQQLREAGVTRYNHNLEASENFFPDIVTTHSWQDRVETIKNLKAAGIQACSGGIMGL
GESWEDRVDLAIALRDLEVESVPLNLLNPRQGTPLGSNDRLDVYEALKCMAIFRFILPEQIIRYAGGREAVMGELQHLGL
KAGINAMLIGHYLTTMGQPPEQDQAMLKSLGLQGGEAPIPGQYSAAQ

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=279, Percent_Identity=34.7670250896057, Blast_Score=190, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6321725, Length=314, Percent_Identity=36.3057324840764, Blast_Score=195, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_SYNP2 (B1XNE1)

Other databases:

- EMBL:   CP000951
- RefSeq:   YP_001733575.1
- ProteinModelPortal:   B1XNE1
- SMR:   B1XNE1
- GeneID:   6056538
- GenomeReviews:   CP000951_GR
- KEGG:   syp:SYNPCC7002_A0309
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK892897
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 39870; Mature: 39870

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQVPIKPTPQTPAPTTAIPNGPALEAWLNDWAQRIINGDRLTRTEALRLTAITGPENIL
CEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHEEEEEECCHHHHH
KLCAAADLIRHECCGNTVDLCSIVNVKSGSCSENCSFCSQSAHHPGEDSPVYGLKTAEEI
HHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHH
IEQAKAAEAAGAKRFCLVSQGRGIKYNSPRDTEFEQILETVRRIQAETAIKPCCALGEVT
HHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PEQAQQLREAGVTRYNHNLEASENFFPDIVTTHSWQDRVETIKNLKAAGIQACSGGIMGL
HHHHHHHHHHCCHHCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
GESWEDRVDLAIALRDLEVESVPLNLLNPRQGTPLGSNDRLDVYEALKCMAIFRFILPEQ
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IIRYAGGREAVMGELQHLGLKAGINAMLIGHYLTTMGQPPEQDQAMLKSLGLQGGEAPIP
HHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCC
GQYSAAQ
CCCCCCC
>Mature Secondary Structure
MVQVPIKPTPQTPAPTTAIPNGPALEAWLNDWAQRIINGDRLTRTEALRLTAITGPENIL
CEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHEEEEEECCHHHHH
KLCAAADLIRHECCGNTVDLCSIVNVKSGSCSENCSFCSQSAHHPGEDSPVYGLKTAEEI
HHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHH
IEQAKAAEAAGAKRFCLVSQGRGIKYNSPRDTEFEQILETVRRIQAETAIKPCCALGEVT
HHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PEQAQQLREAGVTRYNHNLEASENFFPDIVTTHSWQDRVETIKNLKAAGIQACSGGIMGL
HHHHHHHHHHCCHHCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
GESWEDRVDLAIALRDLEVESVPLNLLNPRQGTPLGSNDRLDVYEALKCMAIFRFILPEQ
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IIRYAGGREAVMGELQHLGLKAGINAMLIGHYLTTMGQPPEQDQAMLKSLGLQGGEAPIP
HHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCC
GQYSAAQ
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA