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The map label for this gene is dnaB [H]

Identifier: 170026068

GI number: 170026068

Start: 4246658

End: 4248013

Strand: Reverse

Name: dnaB [H]

Synonym: YPK_3855

Alternate gene names: 170026068

Gene position: 4248013-4246658 (Counterclockwise)

Preceding gene: 170026070

Following gene: 170026067

Centisome position: 90.59

GC content: 51.99

Gene sequence:

>1356_bases
ATGGAAGGGCTGAAGCTTCCGCCGCATTCGCTGGAGGCTGAGCAGTCCGTGTTGGGCGGTTTGATGCTGGATAACGAACG
TTGGGATAACGTATCAGAACGTGTCGCCAGCAAGGACTTTTTCAGCCGCCCGCATCGCCGGATCTTTACTGAAATGCAGC
GCTTGTTGGAAAACAGCAAGCCGATCGACTTGATCACCTTGTCCGAATCGCTAGAGCAAAAAGGCGATTTAGACTCGGTG
GGGGGCTTTGCCTATCTGGCCGAACTATCTAAAAACACGCCAAGTGCGGCGAATATCGGTGCTTATGCCGATATCGTGCG
TGAACGTGCCGTGGTTCGTGAAATGATCTCTGTGGCGAATGAGATCGCCGATGCCGGCTACGATCCCCAAGGGCGCAGCA
GTGAAGATCTGCTGGATTTGGCGGAGTCCAAAGTTTTCCAAATCGCAGAAAGCCGCGCCAGTAAAGATGAAGGTCCTAAA
AGCGTTGATCGCATTCTGGAAGATACCGTCGCCCGTATTGAACAACTTTATCAGCGCCCACACGATGGTGTCACTGGTGT
TTCAACGGGCTTTACTGATCTCGATAAAAAGACCGCTGGGCTACAAAAATCCGATCTGATCATTGTGGCGGCCCGTCCAT
CAATGGGGAAAACCACCTTTGCGATGAACCTGTGTGAAAACGCGGCGATGATGCAGGACAAACCGGTGTTGATCTTCAGC
CTGGAGATGCCCGGCGATCAGATCATGATGCGTATGCTGGCATCCTTGTCACATGTCGATCAGACCCGCATCCGTACCGG
GCAGTTAGATGATGAGGATTGGGCGCGTATTTCCAGCACCATGGGCATATTGATGGAAAAACGCAATATGTACATTGATG
ACTCCTCGGGTCTGACACCAACGGAAGTCCGCTCTCGTGCGCGGCGTATCTTCCGTGAACACGGGGGGTTGAGCCTGATC
ATGATCGACTACCTGCAATTAATGCGGGTGCCGTCCTTGTCGGATAACCGTACGCTCGAAATCGCGGAAATTTCACGCTC
ACTGAAAGCCTTGGCGAAAGAGCTGCAAGTGCCGGTAGTCGCGCTCTCCCAGCTTAACCGTAGTTTGGAGCAGCGTGCCG
ATAAGCGCCCGGTTAACTCCGACTTGCGTGAGTCCGGATCGATTGAGCAGGATGCCGACTTAATCATGTTTATTTACCGT
GATGAGGTTTATCACGAAAACAGTGATGAGAAAGGGATCGCACAAATTATTTTGGGTAAACAGCGTAATGGCCCGATCGG
TTCCGTTCGTCTGAAATTTAACGGTCAGTGGTCACGTTTTGATAACTATGCGGGTCCGCAATATGACGACGAGTAG

Upstream 100 bases:

>100_bases
CTATTCGTCCTTTTTCTATTTGCAATAATAAAGGTAAAGAACACTCTTCATGGCAGCGAAAAAACCAACCAACAAGATGA
CCGAGCCACGAGACCGCCAG

Downstream 100 bases:

>100_bases
TGAATAGCCTCTTGGTATTTGATACTGCCGAGGTGAGCACGGACTACCTGCAACGCCAATCATGTTGGGAATAATATCAA
TTATTAAGGATAAGAAATGA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MEGLKLPPHSLEAEQSVLGGLMLDNERWDNVSERVASKDFFSRPHRRIFTEMQRLLENSKPIDLITLSESLEQKGDLDSV
GGFAYLAELSKNTPSAANIGAYADIVRERAVVREMISVANEIADAGYDPQGRSSEDLLDLAESKVFQIAESRASKDEGPK
SVDRILEDTVARIEQLYQRPHDGVTGVSTGFTDLDKKTAGLQKSDLIIVAARPSMGKTTFAMNLCENAAMMQDKPVLIFS
LEMPGDQIMMRMLASLSHVDQTRIRTGQLDDEDWARISSTMGILMEKRNMYIDDSSGLTPTEVRSRARRIFREHGGLSLI
MIDYLQLMRVPSLSDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYR
DEVYHENSDEKGIAQIILGKQRNGPIGSVRLKFNGQWSRFDNYAGPQYDDE

Sequences:

>Translated_451_residues
MEGLKLPPHSLEAEQSVLGGLMLDNERWDNVSERVASKDFFSRPHRRIFTEMQRLLENSKPIDLITLSESLEQKGDLDSV
GGFAYLAELSKNTPSAANIGAYADIVRERAVVREMISVANEIADAGYDPQGRSSEDLLDLAESKVFQIAESRASKDEGPK
SVDRILEDTVARIEQLYQRPHDGVTGVSTGFTDLDKKTAGLQKSDLIIVAARPSMGKTTFAMNLCENAAMMQDKPVLIFS
LEMPGDQIMMRMLASLSHVDQTRIRTGQLDDEDWARISSTMGILMEKRNMYIDDSSGLTPTEVRSRARRIFREHGGLSLI
MIDYLQLMRVPSLSDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYR
DEVYHENSDEKGIAQIILGKQRNGPIGSVRLKFNGQWSRFDNYAGPQYDDE
>Mature_451_residues
MEGLKLPPHSLEAEQSVLGGLMLDNERWDNVSERVASKDFFSRPHRRIFTEMQRLLENSKPIDLITLSESLEQKGDLDSV
GGFAYLAELSKNTPSAANIGAYADIVRERAVVREMISVANEIADAGYDPQGRSSEDLLDLAESKVFQIAESRASKDEGPK
SVDRILEDTVARIEQLYQRPHDGVTGVSTGFTDLDKKTAGLQKSDLIIVAARPSMGKTTFAMNLCENAAMMQDKPVLIFS
LEMPGDQIMMRMLASLSHVDQTRIRTGQLDDEDWARISSTMGILMEKRNMYIDDSSGLTPTEVRSRARRIFREHGGLSLI
MIDYLQLMRVPSLSDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYR
DEVYHENSDEKGIAQIILGKQRNGPIGSVRLKFNGQWSRFDNYAGPQYDDE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=449, Percent_Identity=86.6369710467706, Blast_Score=808, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50591; Mature: 50591

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGLKLPPHSLEAEQSVLGGLMLDNERWDNVSERVASKDFFSRPHRRIFTEMQRLLENSK
CCCCCCCCCCCHHHHHHHCCHHCCCCHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
PIDLITLSESLEQKGDLDSVGGFAYLAELSKNTPSAANIGAYADIVRERAVVREMISVAN
CEEEEECCHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EIADAGYDPQGRSSEDLLDLAESKVFQIAESRASKDEGPKSVDRILEDTVARIEQLYQRP
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
HDGVTGVSTGFTDLDKKTAGLQKSDLIIVAARPSMGKTTFAMNLCENAAMMQDKPVLIFS
CCCCCCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEE
LEMPGDQIMMRMLASLSHVDQTRIRTGQLDDEDWARISSTMGILMEKRNMYIDDSSGLTP
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCH
TEVRSRARRIFREHGGLSLIMIDYLQLMRVPSLSDNRTLEIAEISRSLKALAKELQVPVV
HHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHCCHHH
ALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDEKGIAQIILGK
HHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEEHHHHCCCCCCCHHHHHHHCC
QRNGPIGSVRLKFNGQWSRFDNYAGPQYDDE
CCCCCCEEEEEEECCCHHHHCCCCCCCCCCC
>Mature Secondary Structure
MEGLKLPPHSLEAEQSVLGGLMLDNERWDNVSERVASKDFFSRPHRRIFTEMQRLLENSK
CCCCCCCCCCCHHHHHHHCCHHCCCCHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
PIDLITLSESLEQKGDLDSVGGFAYLAELSKNTPSAANIGAYADIVRERAVVREMISVAN
CEEEEECCHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EIADAGYDPQGRSSEDLLDLAESKVFQIAESRASKDEGPKSVDRILEDTVARIEQLYQRP
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
HDGVTGVSTGFTDLDKKTAGLQKSDLIIVAARPSMGKTTFAMNLCENAAMMQDKPVLIFS
CCCCCCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEE
LEMPGDQIMMRMLASLSHVDQTRIRTGQLDDEDWARISSTMGILMEKRNMYIDDSSGLTP
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCH
TEVRSRARRIFREHGGLSLIMIDYLQLMRVPSLSDNRTLEIAEISRSLKALAKELQVPVV
HHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHCCHHH
ALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDEKGIAQIILGK
HHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEEHHHHCCCCCCCHHHHHHHCC
QRNGPIGSVRLKFNGQWSRFDNYAGPQYDDE
CCCCCCEEEEEEECCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]