Click here to switch to the map view.

The map label for this gene is phnJ [H]

Identifier: 170025912

GI number: 170025912

Start: 4084742

End: 4085623

Strand: Direct

Name: phnJ [H]

Synonym: YPK_3697

Alternate gene names: 170025912

Gene position: 4084742-4085623 (Clockwise)

Preceding gene: 170025911

Following gene: 170025913

Centisome position: 87.11

GC content: 52.95

Gene sequence:

>882_bases
ATGACTGAGGTTCTCACTGGCTACAACCTGGGTTATTTGGATGAGCAAACCAAGCGTACATTGCGCCGTGCCATACTGAA
AGCCGTCGCAATCCCAGGCTATCAAGTGCCATTTGGTGGGCGTGAGATGCCAATGCCTTACGGCTGGGGGACTGGCGGCA
TTCAGTTGACGGCGTGTCTGATTGGCCACACGGACGTGTTGAAAGTCATTGATCAGGGCGCAGATGACACCACCAATGCC
GTCTCGATCCGCCGCTTTTTCCAACGGGTCAGCGGGGTGGCAACCACAGAGAAAACCACTGAAGCCACATTAATCCAGAC
CCGCCATCGCATACCGGAAACCCCGCTGACAGAAGATCAGATCCTGATTTATCAGGTACCGATCCCAGAACCGCTGCGAT
TTATTGAGCCACGTGAAATCGAGACTCGAAAAATGCATGCACTGGAGGAGTACGGCGTGATGCAGGTAAAACTGTACGAA
GATATCGCCCGTTACGGCCATATCGCCACCACCTATGCCTACCCGGTCAAAGTGAATGATCGCTATGTCATGGACCCTTC
ACCGATCCCGAAATTTGATAATCCCAAAATGCATATGATGCCCGCACTGCAACTGTTCGGGGCTGGTCGTGAGAAACGCC
TTTATGCCCTGCCCCCGTTTACCAAGGTGGAAAGCCTCGATTTTGACGATCATCCGTTTAGCGTACAACAGTGGGATGAC
CCCTGCGCACTGTGTGGTTCGCGCCACAGTTATCTTGATGAAGTGGTGATGGATGACCAAGGTAGCCGCATGTTTGTTTG
CTCGGATACTGATTTTTGCCAGCAGCAACTGGCTCAAGCCAGCGCACAAGAAGCCACATCCCCAACCGAGGTACCTGTCT
GA

Upstream 100 bases:

>100_bases
GCCCTTTTCACACCGCAATTATCGTCTTCTTCATTACCATCATCTTCACAACCACCATCATCTTCACAACCACCATCTTC
TCAACGACAGGAGCCACACC

Downstream 100 bases:

>100_bases
TGATGTCAGCCCAATCTACCCCTCAGCCGCTGTTGTCGGTGGAGAATCTCACTCATTTATATGCGCCGGGCAAAGGATTC
AGTGATGTCTCTTTTCAGCT

Product: phosphonate metabolism PhnJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MTEVLTGYNLGYLDEQTKRTLRRAILKAVAIPGYQVPFGGREMPMPYGWGTGGIQLTACLIGHTDVLKVIDQGADDTTNA
VSIRRFFQRVSGVATTEKTTEATLIQTRHRIPETPLTEDQILIYQVPIPEPLRFIEPREIETRKMHALEEYGVMQVKLYE
DIARYGHIATTYAYPVKVNDRYVMDPSPIPKFDNPKMHMMPALQLFGAGREKRLYALPPFTKVESLDFDDHPFSVQQWDD
PCALCGSRHSYLDEVVMDDQGSRMFVCSDTDFCQQQLAQASAQEATSPTEVPV

Sequences:

>Translated_293_residues
MTEVLTGYNLGYLDEQTKRTLRRAILKAVAIPGYQVPFGGREMPMPYGWGTGGIQLTACLIGHTDVLKVIDQGADDTTNA
VSIRRFFQRVSGVATTEKTTEATLIQTRHRIPETPLTEDQILIYQVPIPEPLRFIEPREIETRKMHALEEYGVMQVKLYE
DIARYGHIATTYAYPVKVNDRYVMDPSPIPKFDNPKMHMMPALQLFGAGREKRLYALPPFTKVESLDFDDHPFSVQQWDD
PCALCGSRHSYLDEVVMDDQGSRMFVCSDTDFCQQQLAQASAQEATSPTEVPV
>Mature_292_residues
TEVLTGYNLGYLDEQTKRTLRRAILKAVAIPGYQVPFGGREMPMPYGWGTGGIQLTACLIGHTDVLKVIDQGADDTTNAV
SIRRFFQRVSGVATTEKTTEATLIQTRHRIPETPLTEDQILIYQVPIPEPLRFIEPREIETRKMHALEEYGVMQVKLYED
IARYGHIATTYAYPVKVNDRYVMDPSPIPKFDNPKMHMMPALQLFGAGREKRLYALPPFTKVESLDFDDHPFSVQQWDDP
CALCGSRHSYLDEVVMDDQGSRMFVCSDTDFCQQQLAQASAQEATSPTEVPV

Specific function: Belongs to an operon involved in alkylphosphonate uptake and C-P lyase. Exact function not known [H]

COG id: COG3627

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnJ family [H]

Homologues:

Organism=Escherichia coli, GI1790536, Length=272, Percent_Identity=86.7647058823529, Blast_Score=513, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010306 [H]

Pfam domain/function: PF06007 PhnJ [H]

EC number: NA

Molecular weight: Translated: 33140; Mature: 33009

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVLTGYNLGYLDEQTKRTLRRAILKAVAIPGYQVPFGGREMPMPYGWGTGGIQLTACL
CCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEEEEEEE
IGHTDVLKVIDQGADDTTNAVSIRRFFQRVSGVATTEKTTEATLIQTRHRIPETPLTEDQ
CCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
ILIYQVPIPEPLRFIEPREIETRKMHALEEYGVMQVKLYEDIARYGHIATTYAYPVKVND
EEEEECCCCCCHHHCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHCCEEEEEEEEEEECC
RYVMDPSPIPKFDNPKMHMMPALQLFGAGREKRLYALPPFTKVESLDFDDHPFSVQQWDD
EEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
PCALCGSRHSYLDEVVMDDQGSRMFVCSDTDFCQQQLAQASAQEATSPTEVPV
HHHHHCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TEVLTGYNLGYLDEQTKRTLRRAILKAVAIPGYQVPFGGREMPMPYGWGTGGIQLTACL
CCEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEEEEEEE
IGHTDVLKVIDQGADDTTNAVSIRRFFQRVSGVATTEKTTEATLIQTRHRIPETPLTEDQ
CCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
ILIYQVPIPEPLRFIEPREIETRKMHALEEYGVMQVKLYEDIARYGHIATTYAYPVKVND
EEEEECCCCCCHHHCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHCCEEEEEEEEEEECC
RYVMDPSPIPKFDNPKMHMMPALQLFGAGREKRLYALPPFTKVESLDFDDHPFSVQQWDD
EEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
PCALCGSRHSYLDEVVMDDQGSRMFVCSDTDFCQQQLAQASAQEATSPTEVPV
HHHHHCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]