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The map label for this gene is radA [H]

Identifier: 170025836

GI number: 170025836

Start: 3995527

End: 3996909

Strand: Reverse

Name: radA [H]

Synonym: YPK_3621

Alternate gene names: 170025836

Gene position: 3996909-3995527 (Counterclockwise)

Preceding gene: 170025837

Following gene: 170025835

Centisome position: 85.23

GC content: 54.59

Gene sequence:

>1383_bases
ATGGCGAAAGCACCCAAACGGGCGTTTGTCTGTAATGAATGTGGCGCAGATTATCCGCGTTGGCAGGGGCAATGCAGCGC
CTGTAACGCCTGGAATACCATTACCGAAGTCAGGCTGGCGGCGGTAGCGTCATCGTCTCGTAATGACCGTTTTGTTGGTT
ATGCTGGCGATGCCGGTGTTAGCCGGGTACAGAAGCTGTCCGATATTAGTCTGGAGGAGCTGCCGCGCTTCTCAACTGGC
TTCCTCGAGTTTGATCGGGTGCTGGGCGGCGGGGTGGTACCGGGCAGTGCCATTCTGATTGGCGGTAACCCCGGTGCAGG
CAAAAGTACTCTATTGCTGCAAACCCTCTGCAAACTGTCGGAAAACATGAAAACCCTGTATGTCACTGGCGAAGAGTCGT
TGCAGCAGGTCGCCATGCGTGCACACCGTCTGGGCCTGCCCACGGCGGGGCTGAATATGTTGTCGGAAACCAGTATCGAG
CAGATTTGCCTGATAGCAGAGCAAGAACAGCCACGGCTGATGGTCATCGACTCCATTCAAGTAATGCATATGGCGGATAT
CCAATCATCGCCGGGCAGTGTGGCGCAAGTGCGCGAAACCGCGGCTTATCTGACTCGTTTTGCTAAAACCCGGGGTGTCG
CCATCGTGATGGTGGGGCATGTGACCAAAGACGGTTCGCTGGCCGGGCCGAAGGTATTAGAACACTGTATTGACTGCTCC
GTGATGCTCGATGGTGATGGCGACTCACGCTTTCGTACTCTGCGTAGCCACAAGAACCGCTTTGGTGCTGTTAACGAACT
GGGTGTGTTTGCCATGACCGAGCAAGGTCTACGGGAAGTCAGTAATCCATCGGCGATCTTTTTGAGCCGTGGCGATGAAG
TGACCGCAGGCAGTTCAGTGATGGTGGTGTGGGAAGGGACTCGCCCATTGCTGGTGGAGATCCAAGCGCTGGTAGATCAT
TCGATGATGTCGAATCCACGCCGGGTCGCTGTCGGGCTGGAACAAAACCGTTTGGCCATCTTATTGGCTGTTTTACACCG
CCACGGTGGTTTGCAGATGTCAGATCAAGACGTGTTCGTTAACGTCGTTGGCGGGGTGAAAGTCACTGAAACCAGCGCTG
ATCTGGCATTGTTGATGTCGCTGGTCTCCAGTCTGCGTGACCGCCCATTACCGCAAGATTTAGTGGTGTTTGGCGAGGTG
GGGCTGGCCGGTGAAATCCGCCCGGTACCAAGTGGTCAGGAGCGTATCTCCGAGGCGGCAAAGCACGGTTTTAAACGTGC
CATCGTGCCTTATGCCAATATGCCTAAAAAACCGCTACCCAATATGCAGGTTTTCGGGGTAAAAAAACTGGCCGATGCGT
TGGCTGTTTTAGAAGATTTATAA

Upstream 100 bases:

>100_bases
ATTCGTTGATAAATCATTTTTTCATAAATCATTTAGTTATAATTAAGTGTTTAATGATTTTTTTTCGTAAAGTATGCGCT
CATAAGATTGAGGTGATGAC

Downstream 100 bases:

>100_bases
CCGTATATACCCTAAATAATTCACGTCGCCGGTGGCTGATCAACGCGCAAGTAACTTGAATTATGACGGGTATATTTATG
GATAATGTCCTATTTAATAG

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MAKAPKRAFVCNECGADYPRWQGQCSACNAWNTITEVRLAAVASSSRNDRFVGYAGDAGVSRVQKLSDISLEELPRFSTG
FLEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLSENMKTLYVTGEESLQQVAMRAHRLGLPTAGLNMLSETSIE
QICLIAEQEQPRLMVIDSIQVMHMADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VMLDGDGDSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTAGSSVMVVWEGTRPLLVEIQALVDH
SMMSNPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALLMSLVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFKRAIVPYANMPKKPLPNMQVFGVKKLADALAVLEDL

Sequences:

>Translated_460_residues
MAKAPKRAFVCNECGADYPRWQGQCSACNAWNTITEVRLAAVASSSRNDRFVGYAGDAGVSRVQKLSDISLEELPRFSTG
FLEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLSENMKTLYVTGEESLQQVAMRAHRLGLPTAGLNMLSETSIE
QICLIAEQEQPRLMVIDSIQVMHMADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VMLDGDGDSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTAGSSVMVVWEGTRPLLVEIQALVDH
SMMSNPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALLMSLVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFKRAIVPYANMPKKPLPNMQVFGVKKLADALAVLEDL
>Mature_459_residues
AKAPKRAFVCNECGADYPRWQGQCSACNAWNTITEVRLAAVASSSRNDRFVGYAGDAGVSRVQKLSDISLEELPRFSTGF
LEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLSENMKTLYVTGEESLQQVAMRAHRLGLPTAGLNMLSETSIEQ
ICLIAEQEQPRLMVIDSIQVMHMADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCSV
MLDGDGDSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTAGSSVMVVWEGTRPLLVEIQALVDHS
MMSNPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALLMSLVSSLRDRPLPQDLVVFGEVG
LAGEIRPVPSGQERISEAAKHGFKRAIVPYANMPKKPLPNMQVFGVKKLADALAVLEDL

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=460, Percent_Identity=91.0869565217391, Blast_Score=868, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49561; Mature: 49430

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAPKRAFVCNECGADYPRWQGQCSACNAWNTITEVRLAAVASSSRNDRFVGYAGDAGV
CCCCCCCCEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHH
SRVQKLSDISLEELPRFSTGFLEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLS
HHHHHHHCCCHHHCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHH
ENMKTLYVTGEESLQQVAMRAHRLGLPTAGLNMLSETSIEQICLIAEQEQPRLMVIDSIQ
CCCEEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHEEEEECCCCCCEEEEECEE
VMHMADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
EEEEHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHCCCE
VMLDGDGDSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTAGSSV
EEEECCCCHHHHHHHHHHHHCCCHHHCCCCEECHHHHHHCCCCCEEEEECCCCEECCCEE
MVVWEGTRPLLVEIQALVDHSMMSNPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFV
EEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEE
NVVGGVKVTETSADLALLMSLVSSLRDRPLPQDLVVFGEVGLAGEIRPVPSGQERISEAA
EEECCEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHH
KHGFKRAIVPYANMPKKPLPNMQVFGVKKLADALAVLEDL
HHCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKAPKRAFVCNECGADYPRWQGQCSACNAWNTITEVRLAAVASSSRNDRFVGYAGDAGV
CCCCCCCEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHH
SRVQKLSDISLEELPRFSTGFLEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLS
HHHHHHHCCCHHHCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHH
ENMKTLYVTGEESLQQVAMRAHRLGLPTAGLNMLSETSIEQICLIAEQEQPRLMVIDSIQ
CCCEEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHEEEEECCCCCCEEEEECEE
VMHMADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
EEEEHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHCCCE
VMLDGDGDSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTAGSSV
EEEECCCCHHHHHHHHHHHHCCCHHHCCCCEECHHHHHHCCCCCEEEEECCCCEECCCEE
MVVWEGTRPLLVEIQALVDHSMMSNPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFV
EEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEE
NVVGGVKVTETSADLALLMSLVSSLRDRPLPQDLVVFGEVGLAGEIRPVPSGQERISEAA
EEECCEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHH
KHGFKRAIVPYANMPKKPLPNMQVFGVKKLADALAVLEDL
HHCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1327967; 8759876; 7610040; 9278503 [H]