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The map label for this gene is murD [H]

Identifier: 170025735

GI number: 170025735

Start: 3866471

End: 3867787

Strand: Reverse

Name: murD [H]

Synonym: YPK_3520

Alternate gene names: 170025735

Gene position: 3867787-3866471 (Counterclockwise)

Preceding gene: 170025736

Following gene: 170025734

Centisome position: 82.48

GC content: 53.91

Gene sequence:

>1317_bases
ATGGTTGATTATCAGGGTAAAAAAGTGGTCATCATCGGGCTGGGGCTAACCGGCCTTTCCTGCGTTGATTTCTTTATAGC
GCGTGGTGTCACGCCGCGCGTGATGGATACCCGCATCAATCCACCGGGCTTAGATCAATTACCCGAAAGTGTCGAACAGC
ATGTGGGTGATTTGAACCAAGAATGGCTACTGGACGCTGATTTAATTGTTGTTAGCCCCGGTATGGCTCTGGCACATCCT
GCATTAAGTGAAGCCGCCGAGGCCGGTGTCGAGATCATTGGCGATATCGAACTGTTCTGCCGTGAAAATCAGGCACCGGT
TGTGGCTATCACTGGCTCCAACGGCAAAAGTACCGTGACGACGCTGGTCGGTGAAATGGCAAAAGCGGCAGGTTGGTCAG
TGGGGGTCGGCGGCAATATCGGCGTTCCGGCACTGACATTGCTGAAGCAAGATAACCAATTAACCGTCTTGGAATTATCA
AGTTTCCAACTGGAAACCACCCACAGCTTGCGGGCCTCTGCTGCGACGATTCTGAACGTGACGGAAGATCATACTGACCG
TTATCCACTCGGTTTGCAGCAATACCGTGCGGCGAAACTGCGGGTGTATGAAAATGCCAAGGTTTGTGTCGTGAATGCCG
ATGATGCACTCACCATGCCGGTCCGTGGTGCTGATAGCCGTTGCATCAGTTTTGGGGTCGATGTCGGTGACTACCACCTG
AATAAGCAGCAGGGTGAAATTTGGTTACGGGTTCGTGGTGAGAAAGTGCTGAACACCCGTGAAATGAAATTAAGTGGGCG
TCATAACTATACCAATGCGCTGGCCGCCCTGGCATTAGCTGATGCCGTGGGTATCCCACGGGCATCCAGTCTGAAAGCCT
TGACGACCTTTTCCGGCTTGCCACACCGCTTCCAATTGGTGCTTGAGCGCCATGGTGTGCGCTGGATCAACGACTCCAAA
GCGACCAACGTGGGCAGCACTGAAGCCGCGCTGGATGGTTTACAGGTTGATGGCACCTTGCATTTATTGTTGGGGGGCGA
CGGTAAATCTGCTGATTTCTCCGGGTTAACGCGCTTCCTGCAAGGGGATCGCATCAAGGTCTACTGTTTTGGCCGCGATG
GCGGTCAATTGGCTGCGTTGCGCCCTGACGTCTCTCAACTTACCGAGACGATGGCGCAGGCTATGGCGTTGGTGGCAAAA
GCTGTGCTACCCGGCGATAGGGTGCTGCTGTCACCTGCGTGTGCCAGCCTGGATCAATTCCGCAGCTTTGAGCATCGTGG
TAATGAGTTTGCTCGTCTGGCAGAGGAGTTAGGCTGA

Upstream 100 bases:

>100_bases
AACTGAAAGGCTGGCCAGAACCACGGGTGATCGTGCGTTTCTGGATTATTTCGCTGATGCTGGTGCTGATTGGCCTGGCG
ACGCTGAAGGTGCGGTAATT

Downstream 100 bases:

>100_bases
TGCGTATGCCGGGGCTGGGGCTGTTTAATACCTTAAAACATTATGTCATGGGGTCGCGGGAGAGCGACACCACCAGCATG
GTGCTTTATGACAGAACCTT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MVDYQGKKVVIIGLGLTGLSCVDFFIARGVTPRVMDTRINPPGLDQLPESVEQHVGDLNQEWLLDADLIVVSPGMALAHP
ALSEAAEAGVEIIGDIELFCRENQAPVVAITGSNGKSTVTTLVGEMAKAAGWSVGVGGNIGVPALTLLKQDNQLTVLELS
SFQLETTHSLRASAATILNVTEDHTDRYPLGLQQYRAAKLRVYENAKVCVVNADDALTMPVRGADSRCISFGVDVGDYHL
NKQQGEIWLRVRGEKVLNTREMKLSGRHNYTNALAALALADAVGIPRASSLKALTTFSGLPHRFQLVLERHGVRWINDSK
ATNVGSTEAALDGLQVDGTLHLLLGGDGKSADFSGLTRFLQGDRIKVYCFGRDGGQLAALRPDVSQLTETMAQAMALVAK
AVLPGDRVLLSPACASLDQFRSFEHRGNEFARLAEELG

Sequences:

>Translated_438_residues
MVDYQGKKVVIIGLGLTGLSCVDFFIARGVTPRVMDTRINPPGLDQLPESVEQHVGDLNQEWLLDADLIVVSPGMALAHP
ALSEAAEAGVEIIGDIELFCRENQAPVVAITGSNGKSTVTTLVGEMAKAAGWSVGVGGNIGVPALTLLKQDNQLTVLELS
SFQLETTHSLRASAATILNVTEDHTDRYPLGLQQYRAAKLRVYENAKVCVVNADDALTMPVRGADSRCISFGVDVGDYHL
NKQQGEIWLRVRGEKVLNTREMKLSGRHNYTNALAALALADAVGIPRASSLKALTTFSGLPHRFQLVLERHGVRWINDSK
ATNVGSTEAALDGLQVDGTLHLLLGGDGKSADFSGLTRFLQGDRIKVYCFGRDGGQLAALRPDVSQLTETMAQAMALVAK
AVLPGDRVLLSPACASLDQFRSFEHRGNEFARLAEELG
>Mature_438_residues
MVDYQGKKVVIIGLGLTGLSCVDFFIARGVTPRVMDTRINPPGLDQLPESVEQHVGDLNQEWLLDADLIVVSPGMALAHP
ALSEAAEAGVEIIGDIELFCRENQAPVVAITGSNGKSTVTTLVGEMAKAAGWSVGVGGNIGVPALTLLKQDNQLTVLELS
SFQLETTHSLRASAATILNVTEDHTDRYPLGLQQYRAAKLRVYENAKVCVVNADDALTMPVRGADSRCISFGVDVGDYHL
NKQQGEIWLRVRGEKVLNTREMKLSGRHNYTNALAALALADAVGIPRASSLKALTTFSGLPHRFQLVLERHGVRWINDSK
ATNVGSTEAALDGLQVDGTLHLLLGGDGKSADFSGLTRFLQGDRIKVYCFGRDGGQLAALRPDVSQLTETMAQAMALVAK
AVLPGDRVLLSPACASLDQFRSFEHRGNEFARLAEELG

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=438, Percent_Identity=81.2785388127854, Blast_Score=724, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 47020; Mature: 47020

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDYQGKKVVIIGLGLTGLSCVDFFIARGVTPRVMDTRINPPGLDQLPESVEQHVGDLNQ
CCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCH
EWLLDADLIVVSPGMALAHPALSEAAEAGVEIIGDIELFCRENQAPVVAITGSNGKSTVT
HHEECCCEEEECCCHHHHCHHHHHHHHCCCEEEECEEEEEECCCCCEEEEECCCCCHHHH
TLVGEMAKAAGWSVGVGGNIGVPALTLLKQDNQLTVLELSSFQLETTHSLRASAATILNV
HHHHHHHHHCCCEECCCCCCCCCEEEEEECCCCEEEEEECCEEEEHHHHHHHHHEEEEEE
TEDHTDRYPLGLQQYRAAKLRVYENAKVCVVNADDALTMPVRGADSRCISFGVDVGDYHL
CCCCCCCCCCCHHHHHHEEEEEECCCEEEEEECCCCEEEECCCCCCCEEEECCCCCCEEC
NKQQGEIWLRVRGEKVLNTREMKLSGRHNYTNALAALALADAVGIPRASSLKALTTFSGL
CCCCCEEEEEECCCHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC
PHRFQLVLERHGVRWINDSKATNVGSTEAALDGLQVDGTLHLLLGGDGKSADFSGLTRFL
CHHHHHHHHHCCCEEECCCCCCCCCCHHHHHCCEEECCEEEEEECCCCCCCCHHHHHHHH
QGDRIKVYCFGRDGGQLAALRPDVSQLTETMAQAMALVAKAVLPGDRVLLSPACASLDQF
CCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHH
RSFEHRGNEFARLAEELG
HHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MVDYQGKKVVIIGLGLTGLSCVDFFIARGVTPRVMDTRINPPGLDQLPESVEQHVGDLNQ
CCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCH
EWLLDADLIVVSPGMALAHPALSEAAEAGVEIIGDIELFCRENQAPVVAITGSNGKSTVT
HHEECCCEEEECCCHHHHCHHHHHHHHCCCEEEECEEEEEECCCCCEEEEECCCCCHHHH
TLVGEMAKAAGWSVGVGGNIGVPALTLLKQDNQLTVLELSSFQLETTHSLRASAATILNV
HHHHHHHHHCCCEECCCCCCCCCEEEEEECCCCEEEEEECCEEEEHHHHHHHHHEEEEEE
TEDHTDRYPLGLQQYRAAKLRVYENAKVCVVNADDALTMPVRGADSRCISFGVDVGDYHL
CCCCCCCCCCCHHHHHHEEEEEECCCEEEEEECCCCEEEECCCCCCCEEEECCCCCCEEC
NKQQGEIWLRVRGEKVLNTREMKLSGRHNYTNALAALALADAVGIPRASSLKALTTFSGL
CCCCCEEEEEECCCHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC
PHRFQLVLERHGVRWINDSKATNVGSTEAALDGLQVDGTLHLLLGGDGKSADFSGLTRFL
CHHHHHHHHHCCCEEECCCCCCCCCCHHHHHCCEEECCEEEEEECCCCCCCCHHHHHHHH
QGDRIKVYCFGRDGGQLAALRPDVSQLTETMAQAMALVAKAVLPGDRVLLSPACASLDQF
CCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHH
RSFEHRGNEFARLAEELG
HHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA