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The map label for this gene is ampD [H]

Identifier: 170025711

GI number: 170025711

Start: 3843076

End: 3843627

Strand: Reverse

Name: ampD [H]

Synonym: YPK_3496

Alternate gene names: 170025711

Gene position: 3843627-3843076 (Counterclockwise)

Preceding gene: 170025716

Following gene: 170025710

Centisome position: 81.96

GC content: 48.55

Gene sequence:

>552_bases
ATGCTGCTGGAAAACGGCTGGATTGTCGGGGTTAAACAGGTGCTGTCCCCCCATTTTGATCTGAGGCCAGAGGGTGAAAT
GCCCTCTCTGCTGGTGATTCATAGTATCAGTCTACCGCCCAGTGAGTTTGGTGGCCCCTATATCGATCAGCTCTTCACCG
GGACACTCAATGCCGATGAGCATCCCTATTTTGCTAGTATTGTACATTTACGCGTCTCGGCCCATTGCTTGATTCGCCGT
GATGGTGAAATCATTCAGTATGTTCCGTTCGATAAACGGGCTTGGCATGCAGGTGTTTCCGTATTTGCTGGGCGTGAGCG
ATGTAACGATTTTTCTATTGGGATCGAGCTGGAAGGGACAGATGTGTTACCGTTTACTCCCGTACAATATCAACGACTGA
CTGAGATCAGTGCGGTTTTATTTGCCCACTATCCAATAACGGTAGAGCGGGTGGCTGGTCACAGTGAGATCGCACCAGGG
CGTAAAACAGACCCAGGGCTGGCCTTTGATTGGTTTTTTTATCAACAAAATTTAGCTAAGTTGCTTGTATGA

Upstream 100 bases:

>100_bases
ATGGCGAATAGACAATCGTCTAAACATGCTACCCTGTTGCAGGCTGAAGTGCCATCAAAGAGCCGTTCAAAGTGAACGAT
CAGGGTGATTGGAGTAAATA

Downstream 100 bases:

>100_bases
GCATGTTGCCGGTGGCATTACACTAGCCAGTGTAATTGTATGCTCGATGTAACCATATGATCTGTTTAATTATATTCTCT
GTGTAACTACATGCTCTAGA

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MLLENGWIVGVKQVLSPHFDLRPEGEMPSLLVIHSISLPPSEFGGPYIDQLFTGTLNADEHPYFASIVHLRVSAHCLIRR
DGEIIQYVPFDKRAWHAGVSVFAGRERCNDFSIGIELEGTDVLPFTPVQYQRLTEISAVLFAHYPITVERVAGHSEIAPG
RKTDPGLAFDWFFYQQNLAKLLV

Sequences:

>Translated_183_residues
MLLENGWIVGVKQVLSPHFDLRPEGEMPSLLVIHSISLPPSEFGGPYIDQLFTGTLNADEHPYFASIVHLRVSAHCLIRR
DGEIIQYVPFDKRAWHAGVSVFAGRERCNDFSIGIELEGTDVLPFTPVQYQRLTEISAVLFAHYPITVERVAGHSEIAPG
RKTDPGLAFDWFFYQQNLAKLLV
>Mature_183_residues
MLLENGWIVGVKQVLSPHFDLRPEGEMPSLLVIHSISLPPSEFGGPYIDQLFTGTLNADEHPYFASIVHLRVSAHCLIRR
DGEIIQYVPFDKRAWHAGVSVFAGRERCNDFSIGIELEGTDVLPFTPVQYQRLTEISAVLFAHYPITVERVAGHSEIAPG
RKTDPGLAFDWFFYQQNLAKLLV

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=180, Percent_Identity=65, Blast_Score=258, Evalue=1e-70,
Organism=Escherichia coli, GI1787092, Length=124, Percent_Identity=40.3225806451613, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 20589; Mature: 20589

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLENGWIVGVKQVLSPHFDLRPEGEMPSLLVIHSISLPPSEFGGPYIDQLFTGTLNADE
CEECCCEEEEHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHCCHHHHHHHCCCCCCCC
HPYFASIVHLRVSAHCLIRRDGEIIQYVPFDKRAWHAGVSVFAGRERCNDFSIGIELEGT
CCHHHHHEEHHEEEEEEEECCCCEEEEECCCCCHHHCCHHEEECCCCCCCEEEEEEECCC
DVLPFTPVQYQRLTEISAVLFAHYPITVERVAGHSEIAPGRKTDPGLAFDWFFYQQNLAK
CCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHH
LLV
HCC
>Mature Secondary Structure
MLLENGWIVGVKQVLSPHFDLRPEGEMPSLLVIHSISLPPSEFGGPYIDQLFTGTLNADE
CEECCCEEEEHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHCCHHHHHHHCCCCCCCC
HPYFASIVHLRVSAHCLIRRDGEIIQYVPFDKRAWHAGVSVFAGRERCNDFSIGIELEGT
CCHHHHHEEHHEEEEEEEECCCCEEEEECCCCCHHHCCHHEEECCCCCCCEEEEEEECCC
DVLPFTPVQYQRLTEISAVLFAHYPITVERVAGHSEIAPGRKTDPGLAFDWFFYQQNLAK
CCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHH
LLV
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2691840; 2607970; 9278503; 7959070 [H]