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The map label for this gene is cueO [H]

Identifier: 170025695

GI number: 170025695

Start: 3822193

End: 3823794

Strand: Reverse

Name: cueO [H]

Synonym: YPK_3480

Alternate gene names: 170025695

Gene position: 3823794-3822193 (Counterclockwise)

Preceding gene: 170025699

Following gene: 170025694

Centisome position: 81.54

GC content: 53.18

Gene sequence:

>1602_bases
ATGCATCGCCGTGATTTTCTTAAGTTAACGGCCGCTCTTGGAGCTGCCACATCACTGCCTTTATGGAGCCGAGCGGCATT
GGCCGCAGATTTTTCCCCGTTACCCATTCCCCCTCTGCTCCAACCGGATGCCAACGGTAAAATTAATCTGAATATTCAGA
CGGGGAGTGTGGTCTGGTTACCTTCCACTGCGACGCAAACCTGGGGCTATAACGGTAATTTATTGGGTCCAGCGATTCGT
TTGCAGCGGGGTAAAGCGGTAACCATTGATATCACTAATGCTTTACCGGAAGCGACCACAGTACATTGGCACGGTTTGGA
GATCCCCGGCGAGGTTGATGGTGGCCCACAGGCGTTGATTCAGCCAGGGGCAAAGCGTCAGGTTACCTTCGCGGTGGAGC
AACCCGCCGCAACGTGTTGGTTCCATCCGCACACTCACAGTAAAACTGGCCACCAAGTGGCGATGGGGTTAGGCGGGTTA
GTCCTGATTGATGACAGCGACAGTGAGAAGCTGCCGTTGCCAAAACAGTGGGGCGTGGACGATATTCCGGTAATTTTGCA
GGATAAATTACTCGATAAACATGGGCAGGTTAACTATCAGCTTGATGTGATGACCGCCGCAGTCGGCTGGTTTGGTGACC
GGATGCTGACTAACGGCGTTCCTTATCCGCAACAAATTACGCCACGTGGCTGGGTGCGATTACGGCTACTTAATGGCTGT
AATGCCCGTTCGCTGAATCTGGCGCTTAGCGATGGCCGGCCAATGTATGTGATTGCCAGCGACGGCGGGTTATTAGCCGA
ACCCGTTGTGGTGCGTGAGTTACCGATATTGATGGGCGAACGTTTCGAAGTGCTGGTGGATACCCGCGATGGTCAGTCTC
TCGATTTGGTCACCTTGCCCGTTACGCAGATGGGCATGACCTTGGCCCCGTTTGATCAGCCGCTGCCCGTGCTACGGATC
CAACCCTCACTGGCGATCGGCAGTCAGGTTTTGCCCGAGTCTCTCGTGGTGATCCCGGAATTAGCCGATGTCACTGGTGT
GCAGGAGCGCTGGTTCCAACTGATGATGGATCGAAAGCTCGATATGCTGGGGATGCAGGCCTTAGTGGCGCGTTATGGCA
TGAAAGCCATGGCCGGTATGAAGATGAATCATGGTGACATGGGGGCGATGGACCATGGCAATAGGCCAGATATGAGCCAG
GGCAAAATGAAAGGCATGGATCATGGCACAATGAACGGTGCGCCAGCCTTTAATTTCAGTCACGCGAATAGGATTAACGG
TAAAGCTTTCTCGATGACCGAACCCGCGTTTGACGCGAAGCAGGGCAAATATGAGAAATGGACCATTTCAGGTGAAGGCG
ACATGATGCTACATCCATTCCATGTTCACGGCACACAGTTCCGTATTTTAACGGAGAACGGCAAACCGCCAGCAGAGCAT
CGCCGGGGATGGAAAGACATAGTACGTGTTGAAGGCGCACGCAGTGAAATATTGGTGCGCTTTAATTATCTCGCCCCTAC
CAGTACGCCTTATATGGCTCACTGCCACTTATTGGAACATGAAGATACTGGCATGATGCTGGGCTTTACCGTCAGCGCCT
GA

Upstream 100 bases:

>100_bases
GGTTTTTATATCCATATATCCATGTGCTATGCAGGTATTTACCTACGGTCAGCATTTACCCATGATCAGCATTTACTTAT
CATCAAGAAGGGAGACGACC

Downstream 100 bases:

>100_bases
GCCACTCCCCGGTCGGTTGACCGGGGTGTTTTTTATAAAACCGCCATCAATGCATCCCACTGCGCATTTTTGTTATACTT
TGTCTCCCCTAAGCTGGCAG

Product: multicopper oxidase

Products: NA

Alternate protein names: Copper efflux oxidase [H]

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MHRRDFLKLTAALGAATSLPLWSRAALAADFSPLPIPPLLQPDANGKINLNIQTGSVVWLPSTATQTWGYNGNLLGPAIR
LQRGKAVTIDITNALPEATTVHWHGLEIPGEVDGGPQALIQPGAKRQVTFAVEQPAATCWFHPHTHSKTGHQVAMGLGGL
VLIDDSDSEKLPLPKQWGVDDIPVILQDKLLDKHGQVNYQLDVMTAAVGWFGDRMLTNGVPYPQQITPRGWVRLRLLNGC
NARSLNLALSDGRPMYVIASDGGLLAEPVVVRELPILMGERFEVLVDTRDGQSLDLVTLPVTQMGMTLAPFDQPLPVLRI
QPSLAIGSQVLPESLVVIPELADVTGVQERWFQLMMDRKLDMLGMQALVARYGMKAMAGMKMNHGDMGAMDHGNRPDMSQ
GKMKGMDHGTMNGAPAFNFSHANRINGKAFSMTEPAFDAKQGKYEKWTISGEGDMMLHPFHVHGTQFRILTENGKPPAEH
RRGWKDIVRVEGARSEILVRFNYLAPTSTPYMAHCHLLEHEDTGMMLGFTVSA

Sequences:

>Translated_533_residues
MHRRDFLKLTAALGAATSLPLWSRAALAADFSPLPIPPLLQPDANGKINLNIQTGSVVWLPSTATQTWGYNGNLLGPAIR
LQRGKAVTIDITNALPEATTVHWHGLEIPGEVDGGPQALIQPGAKRQVTFAVEQPAATCWFHPHTHSKTGHQVAMGLGGL
VLIDDSDSEKLPLPKQWGVDDIPVILQDKLLDKHGQVNYQLDVMTAAVGWFGDRMLTNGVPYPQQITPRGWVRLRLLNGC
NARSLNLALSDGRPMYVIASDGGLLAEPVVVRELPILMGERFEVLVDTRDGQSLDLVTLPVTQMGMTLAPFDQPLPVLRI
QPSLAIGSQVLPESLVVIPELADVTGVQERWFQLMMDRKLDMLGMQALVARYGMKAMAGMKMNHGDMGAMDHGNRPDMSQ
GKMKGMDHGTMNGAPAFNFSHANRINGKAFSMTEPAFDAKQGKYEKWTISGEGDMMLHPFHVHGTQFRILTENGKPPAEH
RRGWKDIVRVEGARSEILVRFNYLAPTSTPYMAHCHLLEHEDTGMMLGFTVSA
>Mature_533_residues
MHRRDFLKLTAALGAATSLPLWSRAALAADFSPLPIPPLLQPDANGKINLNIQTGSVVWLPSTATQTWGYNGNLLGPAIR
LQRGKAVTIDITNALPEATTVHWHGLEIPGEVDGGPQALIQPGAKRQVTFAVEQPAATCWFHPHTHSKTGHQVAMGLGGL
VLIDDSDSEKLPLPKQWGVDDIPVILQDKLLDKHGQVNYQLDVMTAAVGWFGDRMLTNGVPYPQQITPRGWVRLRLLNGC
NARSLNLALSDGRPMYVIASDGGLLAEPVVVRELPILMGERFEVLVDTRDGQSLDLVTLPVTQMGMTLAPFDQPLPVLRI
QPSLAIGSQVLPESLVVIPELADVTGVQERWFQLMMDRKLDMLGMQALVARYGMKAMAGMKMNHGDMGAMDHGNRPDMSQ
GKMKGMDHGTMNGAPAFNFSHANRINGKAFSMTEPAFDAKQGKYEKWTISGEGDMMLHPFHVHGTQFRILTENGKPPAEH
RRGWKDIVRVEGARSEILVRFNYLAPTSTPYMAHCHLLEHEDTGMMLGFTVSA

Specific function: Probably involved in periplasmic detoxification of copper by oxidizing Cu(+) to Cu(2+) and thus preventing its uptake into the cytoplasm. Possesses phenoloxidase and ferroxidase activities and might be involved in the production of polyphenolic compounds

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 plastocyanin-like domains [H]

Homologues:

Organism=Escherichia coli, GI1786314, Length=531, Percent_Identity=64.7834274952919, Blast_Score=700, Evalue=0.0,
Organism=Escherichia coli, GI1789394, Length=526, Percent_Identity=32.5095057034221, Blast_Score=248, Evalue=6e-67,
Organism=Drosophila melanogaster, GI18859919, Length=235, Percent_Identity=27.2340425531915, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001117
- InterPro:   IPR011706
- InterPro:   IPR011707
- InterPro:   IPR002355
- InterPro:   IPR008972
- InterPro:   IPR006311 [H]

Pfam domain/function: PF00394 Cu-oxidase; PF07731 Cu-oxidase_2; PF07732 Cu-oxidase_3 [H]

EC number: NA

Molecular weight: Translated: 58458; Mature: 58458

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00080 MULTICOPPER_OXIDASE2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRRDFLKLTAALGAATSLPLWSRAALAADFSPLPIPPLLQPDANGKINLNIQTGSVVWL
CCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEE
PSTATQTWGYNGNLLGPAIRLQRGKAVTIDITNALPEATTVHWHGLEIPGEVDGGPQALI
CCCCCCCCCCCCCEECCEEEEECCCEEEEEECCCCCCCEEEEEECEECCCCCCCCCHHHC
QPGAKRQVTFAVEQPAATCWFHPHTHSKTGHQVAMGLGGLVLIDDSDSEKLPLPKQWGVD
CCCCCCEEEEEEECCCEEEEECCCCCCCCCCEEEECCCCEEEEECCCCCCCCCCHHCCCC
DIPVILQDKLLDKHGQVNYQLDVMTAAVGWFGDRMLTNGVPYPQQITPRGWVRLRLLNGC
CCCHHHHHHHHCCCCCEEEEEEEEEEHHHHHCCHHHHCCCCCCCCCCCCCEEEEEEECCC
NARSLNLALSDGRPMYVIASDGGLLAEPVVVRELPILMGERFEVLVDTRDGQSLDLVTLP
CCEEEEEEECCCCEEEEEECCCCCEECCCHHHHCCHHHCCCEEEEEECCCCCEEEEEEEC
VTQMGMTLAPFDQPLPVLRIQPSLAIGSQVLPESLVVIPELADVTGVQERWFQLMMDRKL
HHHCCCEECCCCCCCCEEEECCCHHCCCHHCCCCEEEEECHHHHCCHHHHHHHHHHHCCH
DMLGMQALVARYGMKAMAGMKMNHGDMGAMDHGNRPDMSQGKMKGMDHGTMNGAPAFNFS
HHHHHHHHHHHHCHHHHHCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
HANRINGKAFSMTEPAFDAKQGKYEKWTISGEGDMMLHPFHVHGTQFRILTENGKPPAEH
CCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCEEEEEEEECCEEEEEEECCCCCCHHH
RRGWKDIVRVEGARSEILVRFNYLAPTSTPYMAHCHLLEHEDTGMMLGFTVSA
HCCHHHHHEECCCCCEEEEEEEEECCCCCCCEEEEEEEEECCCCEEEEEEECC
>Mature Secondary Structure
MHRRDFLKLTAALGAATSLPLWSRAALAADFSPLPIPPLLQPDANGKINLNIQTGSVVWL
CCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEE
PSTATQTWGYNGNLLGPAIRLQRGKAVTIDITNALPEATTVHWHGLEIPGEVDGGPQALI
CCCCCCCCCCCCCEECCEEEEECCCEEEEEECCCCCCCEEEEEECEECCCCCCCCCHHHC
QPGAKRQVTFAVEQPAATCWFHPHTHSKTGHQVAMGLGGLVLIDDSDSEKLPLPKQWGVD
CCCCCCEEEEEEECCCEEEEECCCCCCCCCCEEEECCCCEEEEECCCCCCCCCCHHCCCC
DIPVILQDKLLDKHGQVNYQLDVMTAAVGWFGDRMLTNGVPYPQQITPRGWVRLRLLNGC
CCCHHHHHHHHCCCCCEEEEEEEEEEHHHHHCCHHHHCCCCCCCCCCCCCEEEEEEECCC
NARSLNLALSDGRPMYVIASDGGLLAEPVVVRELPILMGERFEVLVDTRDGQSLDLVTLP
CCEEEEEEECCCCEEEEEECCCCCEECCCHHHHCCHHHCCCEEEEEECCCCCEEEEEEEC
VTQMGMTLAPFDQPLPVLRIQPSLAIGSQVLPESLVVIPELADVTGVQERWFQLMMDRKL
HHHCCCEECCCCCCCCEEEECCCHHCCCHHCCCCEEEEECHHHHCCHHHHHHHHHHHCCH
DMLGMQALVARYGMKAMAGMKMNHGDMGAMDHGNRPDMSQGKMKGMDHGTMNGAPAFNFS
HHHHHHHHHHHHCHHHHHCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
HANRINGKAFSMTEPAFDAKQGKYEKWTISGEGDMMLHPFHVHGTQFRILTENGKPPAEH
CCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCEEEEEEEECCEEEEEEECCCCCCHHH
RRGWKDIVRVEGARSEILVRFNYLAPTSTPYMAHCHLLEHEDTGMMLGFTVSA
HCCHHHHHEECCCCCEEEEEEEEECCCCCCCEEEEEEEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]