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The map label for this gene is ispD

Identifier: 170025646

GI number: 170025646

Start: 3764446

End: 3765171

Strand: Reverse

Name: ispD

Synonym: YPK_3431

Alternate gene names: 170025646

Gene position: 3765171-3764446 (Counterclockwise)

Preceding gene: 170025647

Following gene: 170025645

Centisome position: 80.29

GC content: 50.83

Gene sequence:

>726_bases
ATGAGTAACTTCGCAGTTTCCCTTCCTGAAGTGATCGCTGTATTACCGGCTGCGGGTATTGGTAGCCGTATGTTGGCGGA
TTGCCCTAAGCAGTATTTAACTGTGGGGGGCAAAACAATCATTGAACATGCTATTTTTTCTTTGCTTCACCACCCACGAA
TTCAGCGGGTTATCGTTGTGATCCATCCGCAGGACACACAATTCTCTAGGTTGTCCGTTGCGCAGGATCCACGTATCAGT
ACAGTTTACGGTGGCGATCAACGGGCTAACTCCGTGATGGCGGGTTTACAATTGGCAGGGCAGGCTGAATGGGTGTTAGT
TCATGATGCGGCACGCCCCTGTTTGCACCTTGATGATCTCAGCCGGCTGTTATCGATTACCGAATGCAGTCAGGTGGGGG
GAATTCTGGCGGCCCCTGTGCGTGATACGATGAAACGTGCCGAGCCGGGTATTCAAGCCATCGCTCATACGGTGGATCGT
CAGGACCTGTGGCATGCGCTGACGCCTCAACTTTTCCCGCTAGAATTATTAAAATTGTGCTTATCCCGTGCGTTAAGAGA
AGGGGTGGCGGTGACTGATGAGGCCTCTGCATTAGAGCATTGCGGTTATCATCCGATATTGGTTACCGGCCGTTCTGATA
ATATTAAAGTGACGCGCCCAGAAGATCTGGCATTGGCGGAGTTTTATTTAACCCAGCGGCAGTCTCTCAATAACGACAGT
CTCTGA

Upstream 100 bases:

>100_bases
GGGCATGATTAAGCCCGGCGAAAGTTTCTATCGTCTGGTTCCTGACCAATCCAGACGCAATGCGGGTACCCCTTCGACAC
AAAATAACGCGCAATAAATA

Downstream 100 bases:

>100_bases
ATAATGACGGTCTCTAAATAACGATAGCCGCTAAACAGCGACAAGTCAGTGAGATGGGTAAGCGGAAGTCGTGAACGTTA
ATCAAGACTAACGGCACTGC

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MSNFAVSLPEVIAVLPAAGIGSRMLADCPKQYLTVGGKTIIEHAIFSLLHHPRIQRVIVVIHPQDTQFSRLSVAQDPRIS
TVYGGDQRANSVMAGLQLAGQAEWVLVHDAARPCLHLDDLSRLLSITECSQVGGILAAPVRDTMKRAEPGIQAIAHTVDR
QDLWHALTPQLFPLELLKLCLSRALREGVAVTDEASALEHCGYHPILVTGRSDNIKVTRPEDLALAEFYLTQRQSLNNDS
L

Sequences:

>Translated_241_residues
MSNFAVSLPEVIAVLPAAGIGSRMLADCPKQYLTVGGKTIIEHAIFSLLHHPRIQRVIVVIHPQDTQFSRLSVAQDPRIS
TVYGGDQRANSVMAGLQLAGQAEWVLVHDAARPCLHLDDLSRLLSITECSQVGGILAAPVRDTMKRAEPGIQAIAHTVDR
QDLWHALTPQLFPLELLKLCLSRALREGVAVTDEASALEHCGYHPILVTGRSDNIKVTRPEDLALAEFYLTQRQSLNNDS
L
>Mature_240_residues
SNFAVSLPEVIAVLPAAGIGSRMLADCPKQYLTVGGKTIIEHAIFSLLHHPRIQRVIVVIHPQDTQFSRLSVAQDPRIST
VYGGDQRANSVMAGLQLAGQAEWVLVHDAARPCLHLDDLSRLLSITECSQVGGILAAPVRDTMKRAEPGIQAIAHTVDRQ
DLWHALTPQLFPLELLKLCLSRALREGVAVTDEASALEHCGYHPILVTGRSDNIKVTRPEDLALAEFYLTQRQSLNNDSL

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Escherichia coli, GI1789104, Length=223, Percent_Identity=68.1614349775785, Blast_Score=330, Evalue=5e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_YERP3 (A7FLX8)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001402256.1
- ProteinModelPortal:   A7FLX8
- SMR:   A7FLX8
- STRING:   A7FLX8
- GeneID:   5387292
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_3299
- NMPDR:   fig|349747.3.peg.3413
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   FPQGAIL
- ProtClustDB:   PRK00155
- BioCyc:   YPSE349747:YPSIP31758_3299-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 26374; Mature: 26243

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNFAVSLPEVIAVLPAAGIGSRMLADCPKQYLTVGGKTIIEHAIFSLLHHPRIQRVIVV
CCCCCCHHHHHHHHHCCCCCCHHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEE
IHPQDTQFSRLSVAQDPRISTVYGGDQRANSVMAGLQLAGQAEWVLVHDAARPCLHLDDL
ECCCCCCHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHH
SRLLSITECSQVGGILAAPVRDTMKRAEPGIQAIAHTVDRQDLWHALTPQLFPLELLKLC
HHHHHHHHHHHHCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LSRALREGVAVTDEASALEHCGYHPILVTGRSDNIKVTRPEDLALAEFYLTQRQSLNNDS
HHHHHHCCCCCCCHHHHHHHCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHCCCCCC
L
C
>Mature Secondary Structure 
SNFAVSLPEVIAVLPAAGIGSRMLADCPKQYLTVGGKTIIEHAIFSLLHHPRIQRVIVV
CCCCCHHHHHHHHHCCCCCCHHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEE
IHPQDTQFSRLSVAQDPRISTVYGGDQRANSVMAGLQLAGQAEWVLVHDAARPCLHLDDL
ECCCCCCHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHH
SRLLSITECSQVGGILAAPVRDTMKRAEPGIQAIAHTVDRQDLWHALTPQLFPLELLKLC
HHHHHHHHHHHHCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LSRALREGVAVTDEASALEHCGYHPILVTGRSDNIKVTRPEDLALAEFYLTQRQSLNNDS
HHHHHHCCCCCCCHHHHHHHCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHCCCCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA