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The map label for this gene is rpoS [H]

Identifier: 170025640

GI number: 170025640

Start: 3759239

End: 3760237

Strand: Reverse

Name: rpoS [H]

Synonym: YPK_3425

Alternate gene names: 170025640

Gene position: 3760237-3759239 (Counterclockwise)

Preceding gene: 170025641

Following gene: 170025638

Centisome position: 80.19

GC content: 48.25

Gene sequence:

>999_bases
ATGAGCCAAAGTACGCTGAAAGTTAACGAGTTGCATGAAGATGCTGATTTTGATGAGAACAGTACGGAAACTGAGATTTT
TGATGAAAAAGCATTAGTAGATGATGAACCTACTGAAAGCGAGTTAGCAGACGATGAGTTGTTGGCGCAAGGTGTTACCC
AGCGTGTGCTGGATGCGACGCAACTCTATCTTGGTGAAATCGGTTATTCGCCACTGCTAACGGCCGAAGAAGAGGTTTAT
TTTGCCCGGCGTGCATTGCGCGGAGACGTACCTTCACGTCGACGTATGATTGAGAGTAACTTGCGGCTGGTGGTGAAGAT
CGCCCGCCGTTACAGTAATCGCGGTTTAGCGCTGTTGGATCTGATAGAAGAGGGTAACCTCGGTTTGATCCGTGCGGTGG
AAAAGTTTGATCCTGAACGCGGTTTCCGTTTTTCCACTTACGCGACTTGGTGGATTCGCCAGACGATTGAACGGGCGATT
ATGAACCAAACCCGTACCATCCGTTTGCCTATCCATATCGTTAAAGAGTTAAACGTTTATTTACGTACAGCACGAGAGCT
TTCTCATAAATTGGATCATGAACCGAGCGCGGAAGAGATTGCAGAGCAACTCGACAAGCCGGTCGATGACGTGAGCCGTA
TGTTACGCCTTAACGAACGTATCACTTCTGTTGATACGCCTTTAGGCGGCGACTCAGAGAAAGCGTTGTTGGATATTCTG
TCTGACGAAAATGAGAACGGTCCGGAAGATACAACACAAGATGACGATATGAAACAGAGTATCGTTAAGTGGTTGTTTGA
GTTGAATGCAAAACAGCGCGAAGTGTTGGCCCGTCGTTTTGGTCTGTTAGGATATGAAGCGGCAACATTGGAAGATGTTG
GCCGAGAAATTGGTTTGACACGCGAGCGTGTCCGCCAGATTCAGGTTGAAGGCTTACGCCGTCTGCGGGAAATCCTGCAA
ACGCAAGGCCTGAGCATCGAGGCGTTGTTCCGCGAATAA

Upstream 100 bases:

>100_bases
CAAGGGGAAATCCGTAAACCCGCTGCGTTACCTTCCGCAGCGATAGATTGGGCAGAATACGCTGTATTCTGCTCGCCGGA
TTCACGGGTAGGAGCAGCAT

Downstream 100 bases:

>100_bases
GTGTGTCTTCACAATGAAAGGCGGTGAACAGTAAGCTTTGTGCAAAATACAAAGTAAACGTGGCCGATATGAAGCACAAA
AAGAGTCGATGAAACACAAC

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSQSTLKVNELHEDADFDENSTETEIFDEKALVDDEPTESELADDELLAQGVTQRVLDATQLYLGEIGYSPLLTAEEEVY
FARRALRGDVPSRRRMIESNLRLVVKIARRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAI
MNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDIL
SDENENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQ
TQGLSIEALFRE

Sequences:

>Translated_332_residues
MSQSTLKVNELHEDADFDENSTETEIFDEKALVDDEPTESELADDELLAQGVTQRVLDATQLYLGEIGYSPLLTAEEEVY
FARRALRGDVPSRRRMIESNLRLVVKIARRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAI
MNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDIL
SDENENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQ
TQGLSIEALFRE
>Mature_331_residues
SQSTLKVNELHEDADFDENSTETEIFDEKALVDDEPTESELADDELLAQGVTQRVLDATQLYLGEIGYSPLLTAEEEVYF
ARRALRGDVPSRRRMIESNLRLVVKIARRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIM
NQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILS
DENENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQT
QGLSIEALFRE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for the normal expression of the enterotoxin, yst, in the stationary phase [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=332, Percent_Identity=92.4698795180723, Blast_Score=615, Evalue=1e-177,
Organism=Escherichia coli, GI1789448, Length=303, Percent_Identity=38.9438943894389, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI1789871, Length=268, Percent_Identity=26.865671641791, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 38225; Mature: 38093

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSTLKVNELHEDADFDENSTETEIFDEKALVDDEPTESELADDELLAQGVTQRVLDAT
CCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QLYLGEIGYSPLLTAEEEVYFARRALRGDVPSRRRMIESNLRLVVKIARRYSNRGLALLD
HHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHH
LIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVY
HHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
LRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDIL
HHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
SDENENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLT
HCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
RERVRQIQVEGLRRLREILQTQGLSIEALFRE
HHHHHHHHHHHHHHHHHHHHHCCCCCEECCCC
>Mature Secondary Structure 
SQSTLKVNELHEDADFDENSTETEIFDEKALVDDEPTESELADDELLAQGVTQRVLDAT
CCCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QLYLGEIGYSPLLTAEEEVYFARRALRGDVPSRRRMIESNLRLVVKIARRYSNRGLALLD
HHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHH
LIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVY
HHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
LRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDIL
HHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
SDENENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLT
HCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
RERVRQIQVEGLRRLREILQTQGLSIEALFRE
HHHHHHHHHHHHHHHHHHHHHCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7729893 [H]