Click here to switch to the map view.

The map label for this gene is pflA [H]

Identifier: 170024904

GI number: 170024904

Start: 2954105

End: 2954902

Strand: Direct

Name: pflA [H]

Synonym: YPK_2679

Alternate gene names: 170024904

Gene position: 2954105-2954902 (Clockwise)

Preceding gene: 170024902

Following gene: 170024907

Centisome position: 62.99

GC content: 46.37

Gene sequence:

>798_bases
ATGAACAAGATAACCGATTGTATTACAACAGACCCCTCCGATCTCGTAGAGGATAAGAAGCCTGTGCTTGGTCGTATTCA
TTCATTCGAATCCTGTGGCACTGTTGATGGCCCAGGTATTAGGTTTATCGTCTTCTTCCAAGGCTGCCTAATGCGCTGCC
TGTATTGTCACAACCGAGATACCTGGGACACCCACGGCGGCAAGGAAGTGACCGTTGAAGAATTAGTTAAAGAAGCAGTC
ACCTATCGCCACTTTATGAATGCTTCGGGTGGCGGTGTCACTGCCTCTGGTGGCGAGGCCATATTGCAGGCTGAGTTTGT
CCGCGATTGGTTCCGAGCCTGTCACAAAGAAGGTATTCACACCTGTTTGGATACCAATGGCTTTGTACGCCGGTATGATC
CCGTCATTGATGAGTTGTTGGATGCAACAGATTTAGTGATGTTAGATTTAAAACAGATGGATGACAGCATTCACCAGAAC
TTGGTTGGCGTATCCAATCACCGGACTTTAGAGTTTGCCCGTTATCTGGCAAAACGTAACCAGAAAACCTGGATCCGCTA
TGTCGTGGTGCCAGGCTGGTCCGACGATGATAAATCAGCACATATGCTGGGTGAATTTACTCAGAACATGAGTAATATCG
AAAAAATAGAATTACTGCCTTACCACGAACTGGGTAAGCACAAATGGATTGCTATGGGGGAAGAATACAAGCTTGATGGC
GTAAAACCGCCAACAAAAGAGATTATGGATCGCGTGAAAGGTATTTTGGAAGGCTATGGCCACAAAGTCATCTACTGA

Upstream 100 bases:

>100_bases
GTATCTGCGCGTCTTGTAAAATGCACAGGTACTGAGTCCCATAAAGGAGTGTCTATATCATGCCTACCATTCAGCACCAA
ACAGTACTAACTGGAACTTT

Downstream 100 bases:

>100_bases
TTTAGAACCCTGTATTGATGCATGTTATTGGCTAAAGTGTACTGTTAACTCATGTGTATTAGGTTTGGCCTAACAACTGC
CGCCATGCTGGATGAATCCG

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MNKITDCITTDPSDLVEDKKPVLGRIHSFESCGTVDGPGIRFIVFFQGCLMRCLYCHNRDTWDTHGGKEVTVEELVKEAV
TYRHFMNASGGGVTASGGEAILQAEFVRDWFRACHKEGIHTCLDTNGFVRRYDPVIDELLDATDLVMLDLKQMDDSIHQN
LVGVSNHRTLEFARYLAKRNQKTWIRYVVVPGWSDDDKSAHMLGEFTQNMSNIEKIELLPYHELGKHKWIAMGEEYKLDG
VKPPTKEIMDRVKGILEGYGHKVIY

Sequences:

>Translated_265_residues
MNKITDCITTDPSDLVEDKKPVLGRIHSFESCGTVDGPGIRFIVFFQGCLMRCLYCHNRDTWDTHGGKEVTVEELVKEAV
TYRHFMNASGGGVTASGGEAILQAEFVRDWFRACHKEGIHTCLDTNGFVRRYDPVIDELLDATDLVMLDLKQMDDSIHQN
LVGVSNHRTLEFARYLAKRNQKTWIRYVVVPGWSDDDKSAHMLGEFTQNMSNIEKIELLPYHELGKHKWIAMGEEYKLDG
VKPPTKEIMDRVKGILEGYGHKVIY
>Mature_265_residues
MNKITDCITTDPSDLVEDKKPVLGRIHSFESCGTVDGPGIRFIVFFQGCLMRCLYCHNRDTWDTHGGKEVTVEELVKEAV
TYRHFMNASGGGVTASGGEAILQAEFVRDWFRACHKEGIHTCLDTNGFVRRYDPVIDELLDATDLVMLDLKQMDDSIHQN
LVGVSNHRTLEFARYLAKRNQKTWIRYVVVPGWSDDDKSAHMLGEFTQNMSNIEKIELLPYHELGKHKWIAMGEEYKLDG
VKPPTKEIMDRVKGILEGYGHKVIY

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=244, Percent_Identity=88.5245901639344, Blast_Score=463, Evalue=1e-132,
Organism=Escherichia coli, GI1790389, Length=283, Percent_Identity=26.1484098939929, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI226510931, Length=185, Percent_Identity=29.7297297297297, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 30226; Mature: 30226

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKITDCITTDPSDLVEDKKPVLGRIHSFESCGTVDGPGIRFIVFFQGCLMRCLYCHNRD
CCCCCHHHCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
TWDTHGGKEVTVEELVKEAVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACHKEGIH
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCHH
TCLDTNGFVRRYDPVIDELLDATDLVMLDLKQMDDSIHQNLVGVSNHRTLEFARYLAKRN
HHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
QKTWIRYVVVPGWSDDDKSAHMLGEFTQNMSNIEKIELLPYHELGKHKWIAMGEEYKLDG
CCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCHHHHCCCCEEEECCCEECCC
VKPPTKEIMDRVKGILEGYGHKVIY
CCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNKITDCITTDPSDLVEDKKPVLGRIHSFESCGTVDGPGIRFIVFFQGCLMRCLYCHNRD
CCCCCHHHCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
TWDTHGGKEVTVEELVKEAVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACHKEGIH
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCHH
TCLDTNGFVRRYDPVIDELLDATDLVMLDLKQMDDSIHQNLVGVSNHRTLEFARYLAKRN
HHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
QKTWIRYVVVPGWSDDDKSAHMLGEFTQNMSNIEKIELLPYHELGKHKWIAMGEEYKLDG
CCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCHHHHCCCCEEEECCCEECCC
VKPPTKEIMDRVKGILEGYGHKVIY
CCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]