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The map label for this gene is chaA [H]

Identifier: 170024462

GI number: 170024462

Start: 2477779

End: 2478879

Strand: Direct

Name: chaA [H]

Synonym: YPK_2233

Alternate gene names: 170024462

Gene position: 2477779-2478879 (Clockwise)

Preceding gene: 170024461

Following gene: 170024463

Centisome position: 52.84

GC content: 48.68

Gene sequence:

>1101_bases
ATGAAGTCGCAACATGATCCTGGCCGATCTAAATCTCGCCACCAAGAATACTCCCTTATTCTACCGATTCTTGCGCTAGT
TATTCTTAACTTCTGGGGCAATACCAGCAATTTCGCTGCCATTATTATAATTAACTTGATTGCGTTGGTTGGTATTCTGA
GCAGTGCTTTCAGTGTCGTGCGTCATGCTGACGTGCTGGCACACCGCTTGGGTGAACCCTATGGGTCGTTGATTCTGAGT
CTGTCGGTGGTGGTACTCGAGGTCAGTCTGATCTCAGCCATGATGGCAACGGGCGATGCGGCACCAGCACTGATGCGCGA
TACTCTCTACTCTATCATTATGATTGTTATTGGCGGTTTGGTTGGGGTTTCATTGCTGCTGGGTGGGCGTAAATTCGCAA
CTCAACACGTTAATCTCGTGGGTATCAAACAATATCTGATGGCTATCTTCCCGTTGGCGATAATTGTGTTGGTTTTACCC
AGCACCCTGCCCGGCGGCAATTTTACCGTGGCACAATCGTTAGTCGTCGCAGCCATTTCCGCAGCAATGTACGGTGTATT
TCTGACAATTCAAACTAAAACACACCAAAGCTTATTTGTATACGAACATGAAGATGAGGGCGATGACCCACACCACGGTA
AACCCTCTTCTCATAGCAGCCTGTGGCATACCGTCTGGTTATTGATACATTTGATCGCCGTGATCGCCGTGACTAAATTT
GATGCCAACCCGCTGGAAGCCCTATTAACTGAATTAAATGCCCCAGCGAAATTTACGGGCTTCCTGATCGCCCTGCTTAT
CCTGTCTCCAGAAGGCTTGGGCGCATTGAAAGCGGTGCTGGCAAATCAGGTACAGCGTGCCATGAATTTGTTCTTCGGCT
CAGTGCTAGCGACAATTTCCCTGACCGTTCCGGCCGTTACACTGATCGCCGTGTTAACGGGTCAGGAACTGAATTTCGGG
CTTGAAGCACCCCATATTGTGGTGATGGTTAGCGTACTGATTTTGTCGAAAATATCGTTCTCTACGGGCAGAACCAATGT
TCTCAACGGTACCGCCCATCTGGCGCTTTTTGCCGCTTATATGATGACCATCATGCTCTAG

Upstream 100 bases:

>100_bases
TACTCTTATGACTACTTAGCCTGTAATATGGCACTTATTTATCGTTAACCGGGCCTAATGCCCGTTTAATACAGTAAAGC
TTTTTAGGAGCCTTCTCAAT

Downstream 100 bases:

>100_bases
CAACCGTCTGAATAGCAAAGGGCAAGCGCTGTTAACGCTTGCCCTTTTTTTGTGCCCAGAAAACCCCCAGCTAGGCTGGG
GGTTCAGTAAAGCTTTCAGC

Product: calcium/sodium:proton antiporter

Products: Na (I) [Periplasm]; Ca (II) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKSQHDPGRSKSRHQEYSLILPILALVILNFWGNTSNFAAIIIINLIALVGILSSAFSVVRHADVLAHRLGEPYGSLILS
LSVVVLEVSLISAMMATGDAAPALMRDTLYSIIMIVIGGLVGVSLLLGGRKFATQHVNLVGIKQYLMAIFPLAIIVLVLP
STLPGGNFTVAQSLVVAAISAAMYGVFLTIQTKTHQSLFVYEHEDEGDDPHHGKPSSHSSLWHTVWLLIHLIAVIAVTKF
DANPLEALLTELNAPAKFTGFLIALLILSPEGLGALKAVLANQVQRAMNLFFGSVLATISLTVPAVTLIAVLTGQELNFG
LEAPHIVVMVSVLILSKISFSTGRTNVLNGTAHLALFAAYMMTIML

Sequences:

>Translated_366_residues
MKSQHDPGRSKSRHQEYSLILPILALVILNFWGNTSNFAAIIIINLIALVGILSSAFSVVRHADVLAHRLGEPYGSLILS
LSVVVLEVSLISAMMATGDAAPALMRDTLYSIIMIVIGGLVGVSLLLGGRKFATQHVNLVGIKQYLMAIFPLAIIVLVLP
STLPGGNFTVAQSLVVAAISAAMYGVFLTIQTKTHQSLFVYEHEDEGDDPHHGKPSSHSSLWHTVWLLIHLIAVIAVTKF
DANPLEALLTELNAPAKFTGFLIALLILSPEGLGALKAVLANQVQRAMNLFFGSVLATISLTVPAVTLIAVLTGQELNFG
LEAPHIVVMVSVLILSKISFSTGRTNVLNGTAHLALFAAYMMTIML
>Mature_366_residues
MKSQHDPGRSKSRHQEYSLILPILALVILNFWGNTSNFAAIIIINLIALVGILSSAFSVVRHADVLAHRLGEPYGSLILS
LSVVVLEVSLISAMMATGDAAPALMRDTLYSIIMIVIGGLVGVSLLLGGRKFATQHVNLVGIKQYLMAIFPLAIIVLVLP
STLPGGNFTVAQSLVVAAISAAMYGVFLTIQTKTHQSLFVYEHEDEGDDPHHGKPSSHSSLWHTVWLLIHLIAVIAVTKF
DANPLEALLTELNAPAKFTGFLIALLILSPEGLGALKAVLANQVQRAMNLFFGSVLATISLTVPAVTLIAVLTGQELNFG
LEAPHIVVMVSVLILSKISFSTGRTNVLNGTAHLALFAAYMMTIML

Specific function: According to PubMed:8496184 the natural activity of this protein is as a calcium/proton antiporter, but it can act as a weak sodium/proton antiporter. The presence of a preferred substrate and normal pH cytoplasmic values might prevent significant sodium/

COG id: COG0387

COG function: function code P; Ca2+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium/potassium/calcium exchanger family [H]

Homologues:

Organism=Escherichia coli, GI1787468, Length=356, Percent_Identity=78.0898876404494, Blast_Score=545, Evalue=1e-156,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004713
- InterPro:   IPR004837 [H]

Pfam domain/function: PF01699 Na_Ca_ex [H]

EC number: NA

Molecular weight: Translated: 39153; Mature: 39153

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSQHDPGRSKSRHQEYSLILPILALVILNFWGNTSNFAAIIIINLIALVGILSSAFSVV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RHADVLAHRLGEPYGSLILSLSVVVLEVSLISAMMATGDAAPALMRDTLYSIIMIVIGGL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
VGVSLLLGGRKFATQHVNLVGIKQYLMAIFPLAIIVLVLPSTLPGGNFTVAQSLVVAAIS
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
AAMYGVFLTIQTKTHQSLFVYEHEDEGDDPHHGKPSSHSSLWHTVWLLIHLIAVIAVTKF
HHHHHHEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
DANPLEALLTELNAPAKFTGFLIALLILSPEGLGALKAVLANQVQRAMNLFFGSVLATIS
CCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTVPAVTLIAVLTGQELNFGLEAPHIVVMVSVLILSKISFSTGRTNVLNGTAHLALFAAY
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
MMTIML
HHHHCC
>Mature Secondary Structure
MKSQHDPGRSKSRHQEYSLILPILALVILNFWGNTSNFAAIIIINLIALVGILSSAFSVV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RHADVLAHRLGEPYGSLILSLSVVVLEVSLISAMMATGDAAPALMRDTLYSIIMIVIGGL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
VGVSLLLGGRKFATQHVNLVGIKQYLMAIFPLAIIVLVLPSTLPGGNFTVAQSLVVAAIS
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
AAMYGVFLTIQTKTHQSLFVYEHEDEGDDPHHGKPSSHSSLWHTVWLLIHLIAVIAVTKF
HHHHHHEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
DANPLEALLTELNAPAKFTGFLIALLILSPEGLGALKAVLANQVQRAMNLFFGSVLATIS
CCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTVPAVTLIAVLTGQELNFGLEAPHIVVMVSVLILSKISFSTGRTNVLNGTAHLALFAAY
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
MMTIML
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm]; Ca (II) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + Na (I) [Periplasm] Ca (II) [Cytoplasm] + Proton [Periplasm] = Ca (II) [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8496184; 8905232; 9278503 [H]