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The map label for this gene is narX [H]

Identifier: 170024461

GI number: 170024461

Start: 2475431

End: 2477212

Strand: Direct

Name: narX [H]

Synonym: YPK_2232

Alternate gene names: 170024461

Gene position: 2475431-2477212 (Clockwise)

Preceding gene: 170024452

Following gene: 170024462

Centisome position: 52.79

GC content: 46.91

Gene sequence:

>1782_bases
ATGAAACGTTATCTGACCCCATTGGTTAATCAGATTGCTTTGTTGATGATACTGCTGGGCATGCTAGGGCTGGCGGGTAT
GGTTATTTCATCATGGATGGCACAAAGCATTCAGGGCAATGCCCACGCCATCAACCAAGCAGGCTCATTGCGTATGCAGA
GCTACAGGTTACTTTCAATGATCCCTTTGGATAGCAATGAGTTATCTTATCTGGTGGCATTGGAGCAAGACAAAACCAGT
CACGACCTGCAACAAGCGCTACTGCGGGAAGGGTTAATGGGTCAATATCAGCAAATTGAACGTTACTGGCAAGACACGCT
GAAGCCTCAGTTACAACAGGCCAAACACCCTGATGAGGTGGCGGCCAGTGTGGCGGATTTTGTTCACCAACTTGATGCTT
TGGTATTAGCCATTGACCATAAAACTGAACAACGGCTGTTACAGGTTACCCTGATCCCGCTGGTATTCAGCCTTCTGACA
CTCAGCCTATTGTTGGCAATGATCTATTATTTGCGCCGCCGGTTACTGCGCCCTTGGCTGAAGTTAATCTCAATGGCGAA
TGCCATTGGCCGAGGTGATTTTAGCCAACGCTATACGCTGGACTATCAGCGTGACGAAATGGGGATGCTGGGTACGGCAT
TGAATCGAATGTCGCAAGAATTGTCATTGATCTACGGCGATCTTGAGCAGCGAGTCGTAAATAAAACCGCTGATTTACAA
CAGAAAAATCAGGTTTTGGCGTTCCTTTATCACAGTAGCCGCCAACTGCATACTCATCAGCCATTAAGTGAGCGTTTAGC
CCCTGTCATTGAACAGCTACAAGCGTTAACCACGCTAGAAAATGTACAAATTTGTTTGTATGAAAACCATCTGTATCGTG
ACCATGTCAATCACAATGCGGATGCAGAATATCTGATGCCTAAAAACCGCCCGGCGTCATTAACGATCAGCGGCCCTGTT
CGCCCCATCAGCCACCCTGAGTTATGGCAATCACTGAGTTGGAGTTTAAGCGATAAACTCGGTCAATATGGTTTATTACT
GGCAAAACAACCCCTCGATAACCCCCTCAATTCTGACCAGCAGCAACTGGTCGATATGTTAGTTGAGCAACTGACCAGCA
CACTGGCGTTAGAGAGCCAAGCCAAACATCAGCAACAGTTATTGTTAATGGAGGAGCGTTCCGCCATTGCGCGGGAACTG
CATGACTCAATCGCTCAATCCCTTTCCTGCCTGAAGATACAGATCAGCTGTTTGCAAATGCAAACTCCGGACTTACCGGA
AAACAGCAACCTATTGATAAAACAAATGCGTGATGAACTCAATATCGCCTACCGTCAATTACGTGAGTTACTGACCACAT
TCCGTCTGAAACTAAATGAAGCCACTCTGGAGGCCGCGTTACAGGCTCTGGTGAATGAGTTCAGCGAAAGATCTGGCCTA
TCGATTACCTTCAAATATCAATTACCTCCAGATCAGGTACCAAACCATCAAGCCATTCATATGGTAAATATTGTCCGTGA
GGCGCTGAATAATATCTATAAGCACGCCCGTGCCAGCGAAGCCACGGTTAACGTCCACTTAGAACAAGGCGATGTGGTGA
TGTCCATAACCGATAATGGTTGTGGGATAGGGCAAGCCAGTGAACAACCCAATCATTATGGTCTGATGATCATGCAAGAT
CGCGCCAACAGTTTACATGGGCACTGTAGTATCAATCAGAAAGAGGGAGGCGGTACTGAGGTTCGAGTGCAATTTACCCC
CGAGAATAATGACCTTGACTGA

Upstream 100 bases:

>100_bases
ATGATGACGGCCGCGGGTATGAAACCCCATTAGCGCTAACACTTCGGCGCTAAAAACGGTAAGGTAACGGCGCTTATTGG
TCAAAGGAATCTCCCACGCG

Downstream 100 bases:

>100_bases
TAATCTATCTTGAAGCACGCCAAAACCACTGATTTCTCGACGACACGCGACTTCAATAGACGCGTCACTTAAATACAATA
CTTAGATAAATACAATACTT

Product: nitrate/nitrite sensor protein NarX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MKRYLTPLVNQIALLMILLGMLGLAGMVISSWMAQSIQGNAHAINQAGSLRMQSYRLLSMIPLDSNELSYLVALEQDKTS
HDLQQALLREGLMGQYQQIERYWQDTLKPQLQQAKHPDEVAASVADFVHQLDALVLAIDHKTEQRLLQVTLIPLVFSLLT
LSLLLAMIYYLRRRLLRPWLKLISMANAIGRGDFSQRYTLDYQRDEMGMLGTALNRMSQELSLIYGDLEQRVVNKTADLQ
QKNQVLAFLYHSSRQLHTHQPLSERLAPVIEQLQALTTLENVQICLYENHLYRDHVNHNADAEYLMPKNRPASLTISGPV
RPISHPELWQSLSWSLSDKLGQYGLLLAKQPLDNPLNSDQQQLVDMLVEQLTSTLALESQAKHQQQLLLMEERSAIAREL
HDSIAQSLSCLKIQISCLQMQTPDLPENSNLLIKQMRDELNIAYRQLRELLTTFRLKLNEATLEAALQALVNEFSERSGL
SITFKYQLPPDQVPNHQAIHMVNIVREALNNIYKHARASEATVNVHLEQGDVVMSITDNGCGIGQASEQPNHYGLMIMQD
RANSLHGHCSINQKEGGGTEVRVQFTPENNDLD

Sequences:

>Translated_593_residues
MKRYLTPLVNQIALLMILLGMLGLAGMVISSWMAQSIQGNAHAINQAGSLRMQSYRLLSMIPLDSNELSYLVALEQDKTS
HDLQQALLREGLMGQYQQIERYWQDTLKPQLQQAKHPDEVAASVADFVHQLDALVLAIDHKTEQRLLQVTLIPLVFSLLT
LSLLLAMIYYLRRRLLRPWLKLISMANAIGRGDFSQRYTLDYQRDEMGMLGTALNRMSQELSLIYGDLEQRVVNKTADLQ
QKNQVLAFLYHSSRQLHTHQPLSERLAPVIEQLQALTTLENVQICLYENHLYRDHVNHNADAEYLMPKNRPASLTISGPV
RPISHPELWQSLSWSLSDKLGQYGLLLAKQPLDNPLNSDQQQLVDMLVEQLTSTLALESQAKHQQQLLLMEERSAIAREL
HDSIAQSLSCLKIQISCLQMQTPDLPENSNLLIKQMRDELNIAYRQLRELLTTFRLKLNEATLEAALQALVNEFSERSGL
SITFKYQLPPDQVPNHQAIHMVNIVREALNNIYKHARASEATVNVHLEQGDVVMSITDNGCGIGQASEQPNHYGLMIMQD
RANSLHGHCSINQKEGGGTEVRVQFTPENNDLD
>Mature_593_residues
MKRYLTPLVNQIALLMILLGMLGLAGMVISSWMAQSIQGNAHAINQAGSLRMQSYRLLSMIPLDSNELSYLVALEQDKTS
HDLQQALLREGLMGQYQQIERYWQDTLKPQLQQAKHPDEVAASVADFVHQLDALVLAIDHKTEQRLLQVTLIPLVFSLLT
LSLLLAMIYYLRRRLLRPWLKLISMANAIGRGDFSQRYTLDYQRDEMGMLGTALNRMSQELSLIYGDLEQRVVNKTADLQ
QKNQVLAFLYHSSRQLHTHQPLSERLAPVIEQLQALTTLENVQICLYENHLYRDHVNHNADAEYLMPKNRPASLTISGPV
RPISHPELWQSLSWSLSDKLGQYGLLLAKQPLDNPLNSDQQQLVDMLVEQLTSTLALESQAKHQQQLLLMEERSAIAREL
HDSIAQSLSCLKIQISCLQMQTPDLPENSNLLIKQMRDELNIAYRQLRELLTTFRLKLNEATLEAALQALVNEFSERSGL
SITFKYQLPPDQVPNHQAIHMVNIVREALNNIYKHARASEATVNVHLEQGDVVMSITDNGCGIGQASEQPNHYGLMIMQD
RANSLHGHCSINQKEGGGTEVRVQFTPENNDLD

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate availability to the narL protein and of both nitrate/nitrite to the narP protein. NarX probably activates narL and narP by phosphorylation in the presence of nitrate. NarX also plays a

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=595, Percent_Identity=47.8991596638655, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI1788812, Length=598, Percent_Identity=31.7725752508361, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI87082311, Length=197, Percent_Identity=29.4416243654822, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR003661
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 67275; Mature: 67275

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRYLTPLVNQIALLMILLGMLGLAGMVISSWMAQSIQGNAHAINQAGSLRMQSYRLLSM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHE
IPLDSNELSYLVALEQDKTSHDLQQALLREGLMGQYQQIERYWQDTLKPQLQQAKHPDEV
ECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AASVADFVHQLDALVLAIDHKTEQRLLQVTLIPLVFSLLTLSLLLAMIYYLRRRLLRPWL
HHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLISMANAIGRGDFSQRYTLDYQRDEMGMLGTALNRMSQELSLIYGDLEQRVVNKTADLQ
HHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKNQVLAFLYHSSRQLHTHQPLSERLAPVIEQLQALTTLENVQICLYENHLYRDHVNHNA
HHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHCCCCC
DAEYLMPKNRPASLTISGPVRPISHPELWQSLSWSLSDKLGQYGLLLAKQPLDNPLNSDQ
CCCEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHEEECCCCCCCCCCCH
QQLVDMLVEQLTSTLALESQAKHQQQLLLMEERSAIARELHDSIAQSLSCLKIQISCLQM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHCC
QTPDLPENSNLLIKQMRDELNIAYRQLRELLTTFRLKLNEATLEAALQALVNEFSERSGL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SITFKYQLPPDQVPNHQAIHMVNIVREALNNIYKHARASEATVNVHLEQGDVVMSITDNG
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEECCC
CGIGQASEQPNHYGLMIMQDRANSLHGHCSINQKEGGGTEVRVQFTPENNDLD
CCCCCCCCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MKRYLTPLVNQIALLMILLGMLGLAGMVISSWMAQSIQGNAHAINQAGSLRMQSYRLLSM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHE
IPLDSNELSYLVALEQDKTSHDLQQALLREGLMGQYQQIERYWQDTLKPQLQQAKHPDEV
ECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AASVADFVHQLDALVLAIDHKTEQRLLQVTLIPLVFSLLTLSLLLAMIYYLRRRLLRPWL
HHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLISMANAIGRGDFSQRYTLDYQRDEMGMLGTALNRMSQELSLIYGDLEQRVVNKTADLQ
HHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKNQVLAFLYHSSRQLHTHQPLSERLAPVIEQLQALTTLENVQICLYENHLYRDHVNHNA
HHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHCCCCC
DAEYLMPKNRPASLTISGPVRPISHPELWQSLSWSLSDKLGQYGLLLAKQPLDNPLNSDQ
CCCEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHEEECCCCCCCCCCCH
QQLVDMLVEQLTSTLALESQAKHQQQLLLMEERSAIARELHDSIAQSLSCLKIQISCLQM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHCC
QTPDLPENSNLLIKQMRDELNIAYRQLRELLTTFRLKLNEATLEAALQALVNEFSERSGL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SITFKYQLPPDQVPNHQAIHMVNIVREALNNIYKHARASEATVNVHLEQGDVVMSITDNG
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEECCC
CGIGQASEQPNHYGLMIMQDRANSLHGHCSINQKEGGGTEVRVQFTPENNDLD
CCCCCCCCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]