Click here to switch to the map view.

The map label for this gene is ansA [H]

Identifier: 170024326

GI number: 170024326

Start: 2322092

End: 2323108

Strand: Direct

Name: ansA [H]

Synonym: YPK_2092

Alternate gene names: 170024326

Gene position: 2322092-2323108 (Clockwise)

Preceding gene: 170024325

Following gene: 170024327

Centisome position: 49.52

GC content: 47.69

Gene sequence:

>1017_bases
ATGCAGAAGAAATCTATTTACGTCGCTTACACGGGTGGCACCATTGGTATGCAACGTTCAGATAATGGGTACATTCCCGT
ATCCGGTCATTTGCAACGCCAACTGGCTTTAATGCCTGAGTTCCATCGTCCGGAGATGCCTGATTTTACTATCCATGAAT
ATGCACCATTAATTGATTCTTCGGATATGACCCCGGAGGATTGGCAACATATTGCCAATGATATTCAGCAAAATTATGAC
CTATATGATGGCTTCGTCATTTTACATGGTACCGATACCATGGCATTTACTGCTTCCGCCCTGTCCTTCATGTTGGAAAA
TCTGGCCAAACCTGTGATTATTACCGGCTCACAAATTCCATTGGCCGAGCTACGCTCTGATGGGCAAACCAATTTGTTGA
ATGCCCTCTATCTGGCAGCCAACCATCCGGTGAATGAAGTCAGCTTATTCTTCAATAACCAACTGTTCCGTGGCAACCGC
ACCACCAAGGCCCATGCCGATGGCTTTGATGCTTTCGCCTCGCCTAATCTTTCCGTATTACTGGAGGCAGGGATCCATAT
TCGCCGCCAATCATCGGTCGTGTCACCCGCCAGTAATGGTCCACTGATTGTCCATCGCATTACGCCACAGCCGATTGGGG
TGGTCACTATCTACCCAGGGATATCCGGTGCCGTAGTGCGGAACTTTCTACTGCAACCCGTGAAAGCCTTAATTTTACGC
TCCTATGGGGTAGGCAACGCACCGCAGAAAGCAGAACTGCTGGATGAACTAAAAAACGCCAGTGACCGTGGTATTGTCGT
CGTGAATTTGACGCAGTGTATTTCCGGCAGTGTCAATATGGGGGGCTATGCAACGGGTAATGCCTTAGCCCAAGCAGGGG
TGATCAGTGGATTTGACATGACAGTCGAAGCGGCGTTGACAAAACTGCATTATTTGTTGAGCCAATCTTTATCTCCTAAC
GAGATTCGCCAACTGATGCAGCAAAATTTACGTGGTGAATTAACCGATACTCAATAA

Upstream 100 bases:

>100_bases
CAGATAAATATTCAGCCCGATACCCATCGGGCTGTTTTTCCTCGCTGAGCTCCTTATAATTCAATCATCCAATCCCAGCA
TTACTTTTCAGGTATCCCCC

Downstream 100 bases:

>100_bases
CTTTTGGGATTTTAATGAAAGCAGCCTTGCTCTTAATCGATTTACAAAACGACTTTTGTCCTGGTGGAGCGTTGGCTGTT
AGTGAAGGTGATGAGGTCAT

Product: cytoplasmic asparaginase I

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MQKKSIYVAYTGGTIGMQRSDNGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYAPLIDSSDMTPEDWQHIANDIQQNYD
LYDGFVILHGTDTMAFTASALSFMLENLAKPVIITGSQIPLAELRSDGQTNLLNALYLAANHPVNEVSLFFNNQLFRGNR
TTKAHADGFDAFASPNLSVLLEAGIHIRRQSSVVSPASNGPLIVHRITPQPIGVVTIYPGISGAVVRNFLLQPVKALILR
SYGVGNAPQKAELLDELKNASDRGIVVVNLTQCISGSVNMGGYATGNALAQAGVISGFDMTVEAALTKLHYLLSQSLSPN
EIRQLMQQNLRGELTDTQ

Sequences:

>Translated_338_residues
MQKKSIYVAYTGGTIGMQRSDNGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYAPLIDSSDMTPEDWQHIANDIQQNYD
LYDGFVILHGTDTMAFTASALSFMLENLAKPVIITGSQIPLAELRSDGQTNLLNALYLAANHPVNEVSLFFNNQLFRGNR
TTKAHADGFDAFASPNLSVLLEAGIHIRRQSSVVSPASNGPLIVHRITPQPIGVVTIYPGISGAVVRNFLLQPVKALILR
SYGVGNAPQKAELLDELKNASDRGIVVVNLTQCISGSVNMGGYATGNALAQAGVISGFDMTVEAALTKLHYLLSQSLSPN
EIRQLMQQNLRGELTDTQ
>Mature_338_residues
MQKKSIYVAYTGGTIGMQRSDNGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYAPLIDSSDMTPEDWQHIANDIQQNYD
LYDGFVILHGTDTMAFTASALSFMLENLAKPVIITGSQIPLAELRSDGQTNLLNALYLAANHPVNEVSLFFNNQLFRGNR
TTKAHADGFDAFASPNLSVLLEAGIHIRRQSSVVSPASNGPLIVHRITPQPIGVVTIYPGISGAVVRNFLLQPVKALILR
SYGVGNAPQKAELLDELKNASDRGIVVVNLTQCISGSVNMGGYATGNALAQAGVISGFDMTVEAALTKLHYLLSQSLSPN
EIRQLMQQNLRGELTDTQ

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=356, Percent_Identity=46.0674157303371, Blast_Score=285, Evalue=6e-77,
Organism=Escherichia coli, GI1788065, Length=335, Percent_Identity=83.2835820895522, Blast_Score=576, Evalue=1e-166,
Organism=Escherichia coli, GI1789327, Length=284, Percent_Identity=28.8732394366197, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17558278, Length=357, Percent_Identity=44.5378151260504, Blast_Score=286, Evalue=8e-78,
Organism=Caenorhabditis elegans, GI17558280, Length=357, Percent_Identity=44.5378151260504, Blast_Score=286, Evalue=9e-78,
Organism=Caenorhabditis elegans, GI193207372, Length=362, Percent_Identity=43.9226519337017, Blast_Score=286, Evalue=9e-78,
Organism=Caenorhabditis elegans, GI193207374, Length=362, Percent_Identity=43.9226519337017, Blast_Score=286, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6320527, Length=171, Percent_Identity=30.4093567251462, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24639609, Length=364, Percent_Identity=43.4065934065934, Blast_Score=293, Evalue=8e-80,
Organism=Drosophila melanogaster, GI24645530, Length=363, Percent_Identity=42.1487603305785, Blast_Score=280, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 36845; Mature: 36845

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKKSIYVAYTGGTIGMQRSDNGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYAPLIDS
CCCCEEEEEEECCEEEEEECCCCEEECCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCC
SDMTPEDWQHIANDIQQNYDLYDGFVILHGTDTMAFTASALSFMLENLAKPVIITGSQIP
CCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCEEEECCCCC
LAELRSDGQTNLLNALYLAANHPVNEVSLFFNNQLFRGNRTTKAHADGFDAFASPNLSVL
HHHHHCCCCHHHHHHHHHHCCCCHHHEEEEECCEEECCCCCCCCCCCCCHHHCCCCCEEE
LEAGIHIRRQSSVVSPASNGPLIVHRITPQPIGVVTIYPGISGAVVRNFLLQPVKALILR
EECCCEEEECCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
SYGVGNAPQKAELLDELKNASDRGIVVVNLTQCISGSVNMGGYATGNALAQAGVISGFDM
HCCCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCCCEECCHHHHHHCCCCCCCH
TVEAALTKLHYLLSQSLSPNEIRQLMQQNLRGELTDTQ
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQKKSIYVAYTGGTIGMQRSDNGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYAPLIDS
CCCCEEEEEEECCEEEEEECCCCEEECCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCC
SDMTPEDWQHIANDIQQNYDLYDGFVILHGTDTMAFTASALSFMLENLAKPVIITGSQIP
CCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCEEEECCCCC
LAELRSDGQTNLLNALYLAANHPVNEVSLFFNNQLFRGNRTTKAHADGFDAFASPNLSVL
HHHHHCCCCHHHHHHHHHHCCCCHHHEEEEECCEEECCCCCCCCCCCCCHHHCCCCCEEE
LEAGIHIRRQSSVVSPASNGPLIVHRITPQPIGVVTIYPGISGAVVRNFLLQPVKALILR
EECCCEEEECCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
SYGVGNAPQKAELLDELKNASDRGIVVVNLTQCISGSVNMGGYATGNALAQAGVISGFDM
HCCCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCCCEECCHHHHHHCCCCCCCH
TVEAALTKLHYLLSQSLSPNEIRQLMQQNLRGELTDTQ
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]