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The map label for this gene is cls [H]

Identifier: 170024298

GI number: 170024298

Start: 2290018

End: 2291478

Strand: Direct

Name: cls [H]

Synonym: YPK_2063

Alternate gene names: 170024298

Gene position: 2290018-2291478 (Clockwise)

Preceding gene: 170024297

Following gene: 170024299

Centisome position: 48.83

GC content: 46.54

Gene sequence:

>1461_bases
ATGACGACATTTTATACCGTAATCAGTTGGCTTTCCGTTTTTGGCTACTGGTTACTCATTGCCGGGGTAACATTGCGTAT
TCTGATGAAACGCAGGGCCGTTCCTTCTGCTATGGCTTGGTTACTGATTATCTACATTCTGCCATTGGTGGGGATAATTG
CTTACCTTTCATTCGGTGAATTGCATTTGGGTAAGCGGCGTGCTGAACGAGCTAAAGCCATGTGGCCATCGACCGCTCGC
TGGCTGAGTGAACTCAAGGAATGCCAACATATCTTTGCTAACTCTAATAGCGAAGTTGCCAGCCCACTGTTTCAATTATG
TGAACGGCGTCAAGGAATAAACGGTGTTAAAGGAAATCAACTACAACTATTAACAACAACCGATGACACACTAAAAGCGC
TAGTACGAGATATTGAACTCGCCCGCCACAATATAGAAATGGTGTTTTATATTTGGCAACCTGGTGGTCTGGTCGATCAG
GTGGCTGAATCGTTGATGGCTGCGGCTCGACGCGGCGTTCATTGCCGGCTGTTACTCGATTCAGCGGGTAGCAAGCAATT
TTTCCGCAGCCCTTATCCCGCAATGATGCGTAATGCAGGTATTGAGGTGGTTGAGGCACTTAAAGTTAATGTATTTCGTA
TGTTCTTGCGCCGAATGGATCTGCGCCAACATCGGAAAATTGTGTTAATCGACAACTATGTTGCTTATACCGGCAGTATG
AACATGGTAGATCCCCGGTTCTTCAAACAAGATGCCGGGGTTGGTCAGTGGATTGATATGATGGCGCGCATGGAAGGCCC
GGTAGCCACAACCCTAGGTATTGTTTATGCCTGTGACTGGGAAATTGAAACAGGCAAACGTATTTTACCCCCGCCCCCCG
ACGCGAATATTATGCCATTCGAAGAAGAGACTGGTCATACGATTCAAGTCATCGCTTCTGGCCCTGGTTTCCCAGAAGAA
ATGATCCACCAGGCATTATTAACAGCTGTGTATGCCGCACGTGAACAGTTAATTATGACTACGCCATATTTTGTCCCCAG
TGATGACTTATTACATGCCATCTGTACCGCGGCACAACGCGGGGTTGATGTCAGTATTATTGTCCCGCGTGAGAATGATT
CGATGATGGTTCGCTGGGCCAGCCGAGCATTCTTCACCGAATTGCTGAATGCAGGCGTTAAAATTTATCAGTTTGAAGGC
GGCCTATTACACAGTAAAAGTGTTCTGGTCGATGGACAGCTAAGTTTAGTCGGCACTGTTAATCTGGATATGCGCAGTTT
GTGGCTGAATTTTGAAATCACACTGGTGATTGATGATGATGGCTTTGGGGCTGATTTAGCACAAGTTCAGGATGATTATA
TCGCCCGCTCTGCCTTATTGGATGGTGAACTGTGGAACAAGCGCCCTCTTTGGCACCGAGTAACTGAGCGTTTATTCTAT
TTTTTCAGCCCATTACTGTAA

Upstream 100 bases:

>100_bases
AAAAGATTTTATACATCAATATAACCGATTAAAGGTTTCACCTTCTCGATACTGTGCGTACACTCTTTAGTGACCGTTTG
TGAACAGGATTTACGCCCTT

Downstream 100 bases:

>100_bases
AAAACCGAAGAATTCATGACCACGAAATTGCAGATTATCTGAATGAGCGGTATTGGGCAGGATAAAAATTGTAAAAACCC
TAGCGAGAATGTTTTAATTA

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTTFYTVISWLSVFGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLSFGELHLGKRRAERAKAMWPSTAR
WLSELKECQHIFANSNSEVASPLFQLCERRQGINGVKGNQLQLLTTTDDTLKALVRDIELARHNIEMVFYIWQPGGLVDQ
VAESLMAAARRGVHCRLLLDSAGSKQFFRSPYPAMMRNAGIEVVEALKVNVFRMFLRRMDLRQHRKIVLIDNYVAYTGSM
NMVDPRFFKQDAGVGQWIDMMARMEGPVATTLGIVYACDWEIETGKRILPPPPDANIMPFEEETGHTIQVIASGPGFPEE
MIHQALLTAVYAAREQLIMTTPYFVPSDDLLHAICTAAQRGVDVSIIVPRENDSMMVRWASRAFFTELLNAGVKIYQFEG
GLLHSKSVLVDGQLSLVGTVNLDMRSLWLNFEITLVIDDDGFGADLAQVQDDYIARSALLDGELWNKRPLWHRVTERLFY
FFSPLL

Sequences:

>Translated_486_residues
MTTFYTVISWLSVFGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLSFGELHLGKRRAERAKAMWPSTAR
WLSELKECQHIFANSNSEVASPLFQLCERRQGINGVKGNQLQLLTTTDDTLKALVRDIELARHNIEMVFYIWQPGGLVDQ
VAESLMAAARRGVHCRLLLDSAGSKQFFRSPYPAMMRNAGIEVVEALKVNVFRMFLRRMDLRQHRKIVLIDNYVAYTGSM
NMVDPRFFKQDAGVGQWIDMMARMEGPVATTLGIVYACDWEIETGKRILPPPPDANIMPFEEETGHTIQVIASGPGFPEE
MIHQALLTAVYAAREQLIMTTPYFVPSDDLLHAICTAAQRGVDVSIIVPRENDSMMVRWASRAFFTELLNAGVKIYQFEG
GLLHSKSVLVDGQLSLVGTVNLDMRSLWLNFEITLVIDDDGFGADLAQVQDDYIARSALLDGELWNKRPLWHRVTERLFY
FFSPLL
>Mature_485_residues
TTFYTVISWLSVFGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLSFGELHLGKRRAERAKAMWPSTARW
LSELKECQHIFANSNSEVASPLFQLCERRQGINGVKGNQLQLLTTTDDTLKALVRDIELARHNIEMVFYIWQPGGLVDQV
AESLMAAARRGVHCRLLLDSAGSKQFFRSPYPAMMRNAGIEVVEALKVNVFRMFLRRMDLRQHRKIVLIDNYVAYTGSMN
MVDPRFFKQDAGVGQWIDMMARMEGPVATTLGIVYACDWEIETGKRILPPPPDANIMPFEEETGHTIQVIASGPGFPEEM
IHQALLTAVYAAREQLIMTTPYFVPSDDLLHAICTAAQRGVDVSIIVPRENDSMMVRWASRAFFTELLNAGVKIYQFEGG
LLHSKSVLVDGQLSLVGTVNLDMRSLWLNFEITLVIDDDGFGADLAQVQDDYIARSALLDGELWNKRPLWHRVTERLFYF
FSPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=486, Percent_Identity=83.7448559670782, Blast_Score=878, Evalue=0.0,
Organism=Escherichia coli, GI1787007, Length=392, Percent_Identity=27.0408163265306, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 55085; Mature: 54954

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFYTVISWLSVFGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLSFGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCH
LHLGKRRAERAKAMWPSTARWLSELKECQHIFANSNSEVASPLFQLCERRQGINGVKGNQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCE
LQLLTTTDDTLKALVRDIELARHNIEMVFYIWQPGGLVDQVAESLMAAARRGVHCRLLLD
EEEEECCCHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEE
SAGSKQFFRSPYPAMMRNAGIEVVEALKVNVFRMFLRRMDLRQHRKIVLIDNYVAYTGSM
CCCCHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECEEEEECCC
NMVDPRFFKQDAGVGQWIDMMARMEGPVATTLGIVYACDWEIETGKRILPPPPDANIMPF
CCCCCHHHHHCCCCHHHHHHHHHCCCCHHHHEEEEEEEEEEECCCCCCCCCCCCCCCCCC
EEETGHTIQVIASGPGFPEEMIHQALLTAVYAAREQLIMTTPYFVPSDDLLHAICTAAQR
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHC
GVDVSIIVPRENDSMMVRWASRAFFTELLNAGVKIYQFEGGLLHSKSVLVDGQLSLVGTV
CCEEEEEEECCCCCEEEEHHHHHHHHHHHHCCEEEEEECCCEEECCEEEECCCEEEEEEE
NLDMRSLWLNFEITLVIDDDGFGADLAQVQDDYIARSALLDGELWNKRPLWHRVTERLFY
CCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
FFSPLL
HHHCCC
>Mature Secondary Structure 
TTFYTVISWLSVFGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLSFGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCH
LHLGKRRAERAKAMWPSTARWLSELKECQHIFANSNSEVASPLFQLCERRQGINGVKGNQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCE
LQLLTTTDDTLKALVRDIELARHNIEMVFYIWQPGGLVDQVAESLMAAARRGVHCRLLLD
EEEEECCCHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEE
SAGSKQFFRSPYPAMMRNAGIEVVEALKVNVFRMFLRRMDLRQHRKIVLIDNYVAYTGSM
CCCCHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECEEEEECCC
NMVDPRFFKQDAGVGQWIDMMARMEGPVATTLGIVYACDWEIETGKRILPPPPDANIMPF
CCCCCHHHHHCCCCHHHHHHHHHCCCCHHHHEEEEEEEEEEECCCCCCCCCCCCCCCCCC
EEETGHTIQVIASGPGFPEEMIHQALLTAVYAAREQLIMTTPYFVPSDDLLHAICTAAQR
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHC
GVDVSIIVPRENDSMMVRWASRAFFTELLNAGVKIYQFEGGLLHSKSVLVDGQLSLVGTV
CCEEEEEEECCCCCEEEEHHHHHHHHHHHHCCEEEEEECCCEEECCEEEECCCEEEEEEE
NLDMRSLWLNFEITLVIDDDGFGADLAQVQDDYIARSALLDGELWNKRPLWHRVTERLFY
CCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
FFSPLL
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA