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The map label for this gene is rbsB [H]

Identifier: 170024200

GI number: 170024200

Start: 2182149

End: 2183099

Strand: Direct

Name: rbsB [H]

Synonym: YPK_1963

Alternate gene names: 170024200

Gene position: 2182149-2183099 (Clockwise)

Preceding gene: 170024199

Following gene: 170024201

Centisome position: 46.53

GC content: 49.32

Gene sequence:

>951_bases
ATGTTTAACAGCAAGCATTTCCTTCTCCTTGTCGCCATGACGTTTGCCACTGTTTTTTCCACCGCGTCGTGGGCCAATAA
TGGGTTAATGACGATCATCATCAACGATCCCTCTAACCCGTATTGGTTCACCGAGGGGCAGGTGGCGAAAGCCACGGCAG
AGAAAATGGGGTACAGCGCTACCGTGAGTGCCCATAAAGGTGATACCAATACCGAAAGCCAGCTTATTGATACGGCGATA
ACCAACAAATCAAAAGCGATTATTCTTGATCCGGCCAATGCTGACGGCTCAATAGGTTCGGTAAAAAAAGCCATCGCCGC
CAATATCCCGGTATTTATAATTAACGCTGAACTCAACCAAAGCGGCCTTGCCAAAGCACAACTGGTCTCCAACAATGCGC
AGGGTGCCGCGCTGGGTGCCATACAGTGGGTTAAAACAGTAGGAGACTCTGGCAAATATGTTGAACTGGTTGGTGCGCCG
TCAGATAACAACGCCGCCACCCGGGCCAACGGCTACAGTAGCGTACTGTCTCAGTATCCAGACCTCAAACGGGTCGGTAA
AGAAGTGGCTAACTGGGATCGTACTCAGGGATACAACAAAATGCAGTCAATGCTTCAGGCAAATCCGGACATAAAAGGGG
TTATCAGCGGGAATGATGAAATGGCGCTCGGGGCCATTGCCGCGTTGAAGGAAGCCGGCAAAATCGACCAAGTGAAAGTA
GGGGCGTTTGATGGTTCACCAGATGCGGTGGATGCCGTGAAAGCCGGTACGTTGGCTTATACTGTGTTGCAACCGGTCGC
TGTTTTTTCAGCAAAAGCCATTGAACAGGCGGATAATTTCCTCAAAACCGGTAAAACGGGCGCTCGGGGTGAAAAACAAC
TTTTTGACTGTATGTTGATCACAAAAGCGAATGTCGACAAATATACATCACCGTTTGTGATGTCAGAGTAA

Upstream 100 bases:

>100_bases
TTAATGTCATTTACGCCGAATTAATGTCATTTACGTTGAGGTCATCAAAAAATCGCGTCATCTACACGCCCTAAATCCTG
CATAAAGTGGAGTATTCATT

Downstream 100 bases:

>100_bases
GTTATTATCTCTCACCGCTGATGAGCCAAACCAGTGAGTCAGCGGTGTCATTTAATCATTATTGAGACTGAGGAGGCGGG
TTATCACCAGCGCCAAAGGT

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MFNSKHFLLLVAMTFATVFSTASWANNGLMTIIINDPSNPYWFTEGQVAKATAEKMGYSATVSAHKGDTNTESQLIDTAI
TNKSKAIILDPANADGSIGSVKKAIAANIPVFIINAELNQSGLAKAQLVSNNAQGAALGAIQWVKTVGDSGKYVELVGAP
SDNNAATRANGYSSVLSQYPDLKRVGKEVANWDRTQGYNKMQSMLQANPDIKGVISGNDEMALGAIAALKEAGKIDQVKV
GAFDGSPDAVDAVKAGTLAYTVLQPVAVFSAKAIEQADNFLKTGKTGARGEKQLFDCMLITKANVDKYTSPFVMSE

Sequences:

>Translated_316_residues
MFNSKHFLLLVAMTFATVFSTASWANNGLMTIIINDPSNPYWFTEGQVAKATAEKMGYSATVSAHKGDTNTESQLIDTAI
TNKSKAIILDPANADGSIGSVKKAIAANIPVFIINAELNQSGLAKAQLVSNNAQGAALGAIQWVKTVGDSGKYVELVGAP
SDNNAATRANGYSSVLSQYPDLKRVGKEVANWDRTQGYNKMQSMLQANPDIKGVISGNDEMALGAIAALKEAGKIDQVKV
GAFDGSPDAVDAVKAGTLAYTVLQPVAVFSAKAIEQADNFLKTGKTGARGEKQLFDCMLITKANVDKYTSPFVMSE
>Mature_316_residues
MFNSKHFLLLVAMTFATVFSTASWANNGLMTIIINDPSNPYWFTEGQVAKATAEKMGYSATVSAHKGDTNTESQLIDTAI
TNKSKAIILDPANADGSIGSVKKAIAANIPVFIINAELNQSGLAKAQLVSNNAQGAALGAIQWVKTVGDSGKYVELVGAP
SDNNAATRANGYSSVLSQYPDLKRVGKEVANWDRTQGYNKMQSMLQANPDIKGVISGNDEMALGAIAALKEAGKIDQVKV
GAFDGSPDAVDAVKAGTLAYTVLQPVAVFSAKAIEQADNFLKTGKTGARGEKQLFDCMLITKANVDKYTSPFVMSE

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=301, Percent_Identity=30.2325581395349, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1790526, Length=306, Percent_Identity=26.4705882352941, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790674, Length=222, Percent_Identity=28.3783783783784, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789990, Length=249, Percent_Identity=27.3092369477912, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 33397; Mature: 33397

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNSKHFLLLVAMTFATVFSTASWANNGLMTIIINDPSNPYWFTEGQVAKATAEKMGYSA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEECCCHHHHHHHHCCCEE
TVSAHKGDTNTESQLIDTAITNKSKAIILDPANADGSIGSVKKAIAANIPVFIINAELNQ
EEEECCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCC
SGLAKAQLVSNNAQGAALGAIQWVKTVGDSGKYVELVGAPSDNNAATRANGYSSVLSQYP
CCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHCC
DLKRVGKEVANWDRTQGYNKMQSMLQANPDIKGVISGNDEMALGAIAALKEAGKIDQVKV
CHHHHHHHHHCCHHCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEE
GAFDGSPDAVDAVKAGTLAYTVLQPVAVFSAKAIEQADNFLKTGKTGARGEKQLFDCMLI
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHEEEE
TKANVDKYTSPFVMSE
EECCCCCCCCCCEECC
>Mature Secondary Structure
MFNSKHFLLLVAMTFATVFSTASWANNGLMTIIINDPSNPYWFTEGQVAKATAEKMGYSA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEECCCHHHHHHHHCCCEE
TVSAHKGDTNTESQLIDTAITNKSKAIILDPANADGSIGSVKKAIAANIPVFIINAELNQ
EEEECCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCC
SGLAKAQLVSNNAQGAALGAIQWVKTVGDSGKYVELVGAPSDNNAATRANGYSSVLSQYP
CCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHCC
DLKRVGKEVANWDRTQGYNKMQSMLQANPDIKGVISGNDEMALGAIAALKEAGKIDQVKV
CHHHHHHHHHCCHHCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEE
GAFDGSPDAVDAVKAGTLAYTVLQPVAVFSAKAIEQADNFLKTGKTGARGEKQLFDCMLI
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHEEEE
TKANVDKYTSPFVMSE
EECCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6313683; 7686882; 9278503; 3011793; 3011794; 8899705; 9298646; 1583688; 7982928; 9641984 [H]