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The map label for this gene is yadI [H]

Identifier: 170023238

GI number: 170023238

Start: 1111264

End: 1111704

Strand: Reverse

Name: yadI [H]

Synonym: YPK_0989

Alternate gene names: 170023238

Gene position: 1111704-1111264 (Counterclockwise)

Preceding gene: 170023239

Following gene: 170023237

Centisome position: 23.71

GC content: 50.34

Gene sequence:

>441_bases
ATGATTGGGATTGTTGTTTCTGGCCACATTAATTTTGCTTCTGGTATGGCTTCCGCAGTACGGGCGATTACCGGTGAGCA
ATCTCAAATGCTGTTCATTGATTTCGTTGAAAGTATGTCAACAGATATGCTGGAAAGCGAACTGCGCCGTGCGGCACATG
AGGTAGACAGCGGCGAAGGTGTACTGTTTTTAACCGATATTCCCGGTGGCTCACCTTGTAATCGCGCACTGAATATTTTG
ATGAGCGGCAGCAACGTTGAGCTGCTTGCCGGGGTTAACCTGCCAATGATTGCGAATGCCGCCTTTGAACGTGAAGGGGC
TTCACTGTCAGAGCTGGTCGAAATACTGATGGATATTGGCGCGTCCAGTGTACAGAACCTACGGGCTGAGCTGGACACCG
CTCTGGTTACTGATAGCGGCGAAAACGAGGATGGGCTCTAA

Upstream 100 bases:

>100_bases
CTGGGCATTGTCTAACCGTTATCGCCATCGCCGCATCGTGGCTTTGCATGATGCGGCGTAACAGAGCAGCCTGAATATCC
AGGCAGTAAGGATGTTGTAA

Downstream 100 bases:

>100_bases
TTATGCGTACTGCCTATCTCGCTGATCGCACTTTTACTCCGCAAGGCATTGAAACCGGCGTCGCGGTTATCGTCGAACAA
GGCGAGATTGTGGCAGTAAC

Product: PTS system fructose subfamily IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: Putative PTS system EIIA component [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MIGIVVSGHINFASGMASAVRAITGEQSQMLFIDFVESMSTDMLESELRRAAHEVDSGEGVLFLTDIPGGSPCNRALNIL
MSGSNVELLAGVNLPMIANAAFEREGASLSELVEILMDIGASSVQNLRAELDTALVTDSGENEDGL

Sequences:

>Translated_146_residues
MIGIVVSGHINFASGMASAVRAITGEQSQMLFIDFVESMSTDMLESELRRAAHEVDSGEGVLFLTDIPGGSPCNRALNIL
MSGSNVELLAGVNLPMIANAAFEREGASLSELVEILMDIGASSVQNLRAELDTALVTDSGENEDGL
>Mature_146_residues
MIGIVVSGHINFASGMASAVRAITGEQSQMLFIDFVESMSTDMLESELRRAAHEVDSGEGVLFLTDIPGGSPCNRALNIL
MSGSNVELLAGVNLPMIANAAFEREGASLSELVEILMDIGASSVQNLRAELDTALVTDSGENEDGL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1786321, Length=127, Percent_Identity=35.4330708661417, Blast_Score=76, Evalue=7e-16,
Organism=Escherichia coli, GI1788120, Length=130, Percent_Identity=33.0769230769231, Blast_Score=74, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701 [H]

Pfam domain/function: PF03610 EIIA-man [H]

EC number: 2.7.1.69

Molecular weight: Translated: 15345; Mature: 15345

Theoretical pI: Translated: 3.81; Mature: 3.81

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGIVVSGHINFASGMASAVRAITGEQSQMLFIDFVESMSTDMLESELRRAAHEVDSGEG
CEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VLFLTDIPGGSPCNRALNILMSGSNVELLAGVNLPMIANAAFEREGASLSELVEILMDIG
EEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHCC
ASSVQNLRAELDTALVTDSGENEDGL
HHHHHHHHHHHHHHEEECCCCCCCCC
>Mature Secondary Structure
MIGIVVSGHINFASGMASAVRAITGEQSQMLFIDFVESMSTDMLESELRRAAHEVDSGEG
CEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VLFLTDIPGGSPCNRALNILMSGSNVELLAGVNLPMIANAAFEREGASLSELVEILMDIG
EEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHCC
ASSVQNLRAELDTALVTDSGENEDGL
HHHHHHHHHHHHHHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503 [H]