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The map label for this gene is dam [C]

Identifier: 170023137

GI number: 170023137

Start: 1011942

End: 1012814

Strand: Direct

Name: dam [C]

Synonym: YPK_0888

Alternate gene names: 170023137

Gene position: 1011942-1012814 (Clockwise)

Preceding gene: 170023136

Following gene: 170023138

Centisome position: 21.58

GC content: 40.09

Gene sequence:

>873_bases
ATGAAAGAAACAAAACTAAATCGCTCACCACTTAAGTGGGCTGGCTCAAAGGCGCGTATTATGCCTGTCCTACGTCCACA
TTTACCTGCCGGTAAGCGATTGGTGGAGCCGTTTGCTGGTTCATGCTCTGTCATGTTGAATACTGATTATGATGAGTATT
TGATTGCTGATATTAATGCGCATCTGATTAATTTTTATAATATGGCAAGGTGTGAAACTAATGATTTAATAAATATGGCT
AGCTCACTATTTTTCACTGCAAACACACCAGAACAGTATTATATATTTCGTAATATTTTCAACTCGGGTAATCGTGATGA
GCTATCAATGGCTGTGATTTTTCTTTATTTAAATCGTCATTGTTTTAACGGACTATGCAGATATAACAAATTGGGTGGTT
TTAATGTCCCCTATGGTAAGTACAAATCTCCTTATTTTCCAGAAGCTGAAATACGTTTCTTTGCCGAGAAAGCCAAGAAA
GCAATATTTATATGTTGTGACTTCTCCGAAGCATTAGAGATGACTTTGCCCGGAGATGTGATTTATTGTGATCCGCCTTA
TATCCCTGCATCTATCACGGCTAACTTTACTGGCTATCATGCTGATGGATTTAATAACGACCAGCAATACCGACTATCCG
TGTTATTGGCGCGCGCTGCTGAAAATGGCTGTCATGCTATTGCTTCAAATAGTGACACGCATATTAGCCGCGATCTTTAT
CACAACTTTACTCTCCACGATATCGTCGCGCCTCGCTCTGTCAATTGCAAGGGTGAAGGTCGTAGCAGTGTGAGTGAAAT
AATAGCGACTAAATCCCCAGCCTCAGTATCTGAGAAAATATATCGGGCTTCTAATAAGGCGATCCCATTTTGA

Upstream 100 bases:

>100_bases
TAGTGATGATTGCACCATAAACATTAAGAATTAACTATAACTGTAATACATGGCGTTTTACTCGCCGGGTTTCGTATTGC
CTAAAAACGGGAGTTATATA

Downstream 100 bases:

>100_bases
ATAATAATATTAAATCAATCCGGTTTATAGGAGTTGATTTGGCTAATCCCGGCAGTGAACACACTGTGATGTCAGTCGAA
TCAATGGAGCTAATGCTCAA

Product: DNA adenine methylase

Products: NA

Alternate protein names: M.Eco67Dam; ORF1-EC67 DAM [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MKETKLNRSPLKWAGSKARIMPVLRPHLPAGKRLVEPFAGSCSVMLNTDYDEYLIADINAHLINFYNMARCETNDLINMA
SSLFFTANTPEQYYIFRNIFNSGNRDELSMAVIFLYLNRHCFNGLCRYNKLGGFNVPYGKYKSPYFPEAEIRFFAEKAKK
AIFICCDFSEALEMTLPGDVIYCDPPYIPASITANFTGYHADGFNNDQQYRLSVLLARAAENGCHAIASNSDTHISRDLY
HNFTLHDIVAPRSVNCKGEGRSSVSEIIATKSPASVSEKIYRASNKAIPF

Sequences:

>Translated_290_residues
MKETKLNRSPLKWAGSKARIMPVLRPHLPAGKRLVEPFAGSCSVMLNTDYDEYLIADINAHLINFYNMARCETNDLINMA
SSLFFTANTPEQYYIFRNIFNSGNRDELSMAVIFLYLNRHCFNGLCRYNKLGGFNVPYGKYKSPYFPEAEIRFFAEKAKK
AIFICCDFSEALEMTLPGDVIYCDPPYIPASITANFTGYHADGFNNDQQYRLSVLLARAAENGCHAIASNSDTHISRDLY
HNFTLHDIVAPRSVNCKGEGRSSVSEIIATKSPASVSEKIYRASNKAIPF
>Mature_290_residues
MKETKLNRSPLKWAGSKARIMPVLRPHLPAGKRLVEPFAGSCSVMLNTDYDEYLIADINAHLINFYNMARCETNDLINMA
SSLFFTANTPEQYYIFRNIFNSGNRDELSMAVIFLYLNRHCFNGLCRYNKLGGFNVPYGKYKSPYFPEAEIRFFAEKAKK
AIFICCDFSEALEMTLPGDVIYCDPPYIPASITANFTGYHADGFNNDQQYRLSVLLARAAENGCHAIASNSDTHISRDLY
HNFTLHDIVAPRSVNCKGEGRSSVSEIIATKSPASVSEKIYRASNKAIPF

Specific function: Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. May play a regulatory role in the functions of the retron [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=274, Percent_Identity=45.2554744525547, Blast_Score=249, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 32686; Mature: 32686

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKETKLNRSPLKWAGSKARIMPVLRPHLPAGKRLVEPFAGSCSVMLNTDYDEYLIADINA
CCCCCCCCCCCHHCCCCCEEEEEECCCCCCCHHHHHCCCCCEEEEEECCCCCEEEEECHH
HLINFYNMARCETNDLINMASSLFFTANTPEQYYIFRNIFNSGNRDELSMAVIFLYLNRH
HHHHHHHHHHCCHHHHHHHHHHEEEECCCCCCEEHHHHHHCCCCCCHHHHHHHHHHHHHH
CFNGLCRYNKLGGFNVPYGKYKSPYFPEAEIRFFAEKAKKAIFICCDFSEALEMTLPGDV
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHEECCCCE
IYCDPPYIPASITANFTGYHADGFNNDQQYRLSVLLARAAENGCHAIASNSDTHISRDLY
EEECCCCCCCEEEECCCEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHH
HNFTLHDIVAPRSVNCKGEGRSSVSEIIATKSPASVSEKIYRASNKAIPF
HCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKETKLNRSPLKWAGSKARIMPVLRPHLPAGKRLVEPFAGSCSVMLNTDYDEYLIADINA
CCCCCCCCCCCHHCCCCCEEEEEECCCCCCCHHHHHCCCCCEEEEEECCCCCEEEEECHH
HLINFYNMARCETNDLINMASSLFFTANTPEQYYIFRNIFNSGNRDELSMAVIFLYLNRH
HHHHHHHHHHCCHHHHHHHHHHEEEECCCCCCEEHHHHHHCCCCCCHHHHHHHHHHHHHH
CFNGLCRYNKLGGFNVPYGKYKSPYFPEAEIRFFAEKAKKAIFICCDFSEALEMTLPGDV
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHEECCCCE
IYCDPPYIPASITANFTGYHADGFNNDQQYRLSVLLARAAENGCHAIASNSDTHISRDLY
EEECCCCCCCEEEECCCEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHH
HNFTLHDIVAPRSVNCKGEGRSSVSEIIATKSPASVSEKIYRASNKAIPF
HCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1701261 [H]