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The map label for this gene is 170023004

Identifier: 170023004

GI number: 170023004

Start: 848978

End: 849742

Strand: Direct

Name: 170023004

Synonym: YPK_0755

Alternate gene names: NA

Gene position: 848978-849742 (Clockwise)

Preceding gene: 170023003

Following gene: 170023005

Centisome position: 18.1

GC content: 48.89

Gene sequence:

>765_bases
ATGATTGAACGCCTTTTTCGATTGATAAAACCGGGAACGCGTCAGCAAATGGTGTGTTTTCACCATGCAGGTGGTTCAGC
GGAGTATTTTTTGCCGTGGAAAACGGATCTGCCACCGGACACCGCCTTGTATGCCATTCAACTTCCTGGGCATGGCGGGC
GGATTAACGAATCGCTATTGCACAATTTGCATCAAATGGCTGATGTCATTGCTCAGAGTTATGTTTACCTGCCTGTGCGT
GACACGTTTTTCTTTGGCCACAGCATGGGGGCCGCTTTGGCTTTTGAGGTGGCATTGCGCTTACAAAAGGCGGGGCGAGG
CATTACCACGCTGTACGTCTCCGGGCGATTACCTCCGCATAAAAATACCGATACGGATTTTTACCGCCAGAGTGATGTGA
GTATCTTGAACAAAATCACCCAATTGGGTGGCAGCACATTGGACTACCAACAGCCAGAACTGAAAGACCTGCTATTACCT
ATTTTTAGGGCGGATTTCACCGCGATAGAGACCTACCAGCGCCAATGTACCGAACTGTTGAATTGTTCGGTTATGGCCTG
TGTGGGAGAGCAGGATACCGAAGTGTCGGTGAGGCAAATGAGTGAGTGGTGTCAGGTGACGAAGGGGGCTTTTCGGCTAC
AGGTGTTCCCCGGTGGGCATTTTTATCTTAATCATGCCCAAAAAAGATTATTGGCATTCATCAACCAGTCTGTTGCCGCG
CAGCAATCAAGCAAGGAATGCAAGGAGTATCACGATGAAATATAA

Upstream 100 bases:

>100_bases
ATGGAAAAAGTCGGCAATCCAGAAAGTGTGCATTTTGCCGAACCGATGATGGCGTCACCTAAGGTGGCGACATTTTTATC
TCAATGGCAGGGAGAGCGTG

Downstream 100 bases:

>100_bases
CGCAAATATTACCAGTTTATTTGGGTTGTCCAGTCTATTTGGATTGTATTGTGCGCAAGGTGTCCCCCTCGGGTTGGCTA
TCTTTGCCCTACCGGGAATT

Product: thioesterase

Products: NA

Alternate protein names: ORF-1; Protein X [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MIERLFRLIKPGTRQQMVCFHHAGGSAEYFLPWKTDLPPDTALYAIQLPGHGGRINESLLHNLHQMADVIAQSYVYLPVR
DTFFFGHSMGAALAFEVALRLQKAGRGITTLYVSGRLPPHKNTDTDFYRQSDVSILNKITQLGGSTLDYQQPELKDLLLP
IFRADFTAIETYQRQCTELLNCSVMACVGEQDTEVSVRQMSEWCQVTKGAFRLQVFPGGHFYLNHAQKRLLAFINQSVAA
QQSSKECKEYHDEI

Sequences:

>Translated_254_residues
MIERLFRLIKPGTRQQMVCFHHAGGSAEYFLPWKTDLPPDTALYAIQLPGHGGRINESLLHNLHQMADVIAQSYVYLPVR
DTFFFGHSMGAALAFEVALRLQKAGRGITTLYVSGRLPPHKNTDTDFYRQSDVSILNKITQLGGSTLDYQQPELKDLLLP
IFRADFTAIETYQRQCTELLNCSVMACVGEQDTEVSVRQMSEWCQVTKGAFRLQVFPGGHFYLNHAQKRLLAFINQSVAA
QQSSKECKEYHDEI
>Mature_254_residues
MIERLFRLIKPGTRQQMVCFHHAGGSAEYFLPWKTDLPPDTALYAIQLPGHGGRINESLLHNLHQMADVIAQSYVYLPVR
DTFFFGHSMGAALAFEVALRLQKAGRGITTLYVSGRLPPHKNTDTDFYRQSDVSILNKITQLGGSTLDYQQPELKDLLLP
IFRADFTAIETYQRQCTELLNCSVMACVGEQDTEVSVRQMSEWCQVTKGAFRLQVFPGGHFYLNHAQKRLLAFINQSVAA
QQSSKECKEYHDEI

Specific function: Probable thioesterase involved in the biosynthesis of candicidin. Could release the macrolide ring from the polyketide synthase [H]

COG id: COG3208

COG function: function code Q; Predicted thioesterase involved in non-ribosomal peptide biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI89257335, Length=244, Percent_Identity=27.4590163934426, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI8922871, Length=202, Percent_Identity=28.2178217821782, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR010693
- InterPro:   IPR012223
- InterPro:   IPR001031 [H]

Pfam domain/function: PF06902 DUF1271; PF00975 Thioesterase [H]

EC number: NA

Molecular weight: Translated: 28788; Mature: 28788

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERLFRLIKPGTRQQMVCFHHAGGSAEYFLPWKTDLPPDTALYAIQLPGHGGRINESLL
CHHHHHHHHCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCHHHH
HNLHQMADVIAQSYVYLPVRDTFFFGHSMGAALAFEVALRLQKAGRGITTLYVSGRLPPH
HHHHHHHHHHHHCEEEEEEHHHEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
KNTDTDFYRQSDVSILNKITQLGGSTLDYQQPELKDLLLPIFRADFTAIETYQRQCTELL
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NCSVMACVGEQDTEVSVRQMSEWCQVTKGAFRLQVFPGGHFYLNHAQKRLLAFINQSVAA
CCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHH
QQSSKECKEYHDEI
HHHHHHHHHHHCCC
>Mature Secondary Structure
MIERLFRLIKPGTRQQMVCFHHAGGSAEYFLPWKTDLPPDTALYAIQLPGHGGRINESLL
CHHHHHHHHCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCHHHH
HNLHQMADVIAQSYVYLPVRDTFFFGHSMGAALAFEVALRLQKAGRGITTLYVSGRLPPH
HHHHHHHHHHHHCEEEEEEHHHEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
KNTDTDFYRQSDVSILNKITQLGGSTLDYQQPELKDLLLPIFRADFTAIETYQRQCTELL
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NCSVMACVGEQDTEVSVRQMSEWCQVTKGAFRLQVFPGGHFYLNHAQKRLLAFINQSVAA
CCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHH
QQSSKECKEYHDEI
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8472954 [H]