Click here to switch to the map view.

The map label for this gene is thiE [H]

Identifier: 170022604

GI number: 170022604

Start: 371326

End: 372000

Strand: Reverse

Name: thiE [H]

Synonym: YPK_0346

Alternate gene names: 170022604

Gene position: 372000-371326 (Counterclockwise)

Preceding gene: 170022605

Following gene: 170022603

Centisome position: 7.93

GC content: 53.19

Gene sequence:

>675_bases
ATGAGCCAACTTGATGCCCTCTCTCCTGTGGCTACACCAGGTTTCCCCTCCACGGAACAGCGTCTTGGGCTCTACCCAGT
GGTGGACTCATTATTATGGATAGAACGCCTGCTGGCAGCCGGAGTCACGACGCTACAACTGCGCATTAAAAATGCAGACG
ATGCTCAGGTGGAGCAAGATATTGTCGCGGCCATTGAATTAGGAAAACGCTATCAGGCGCGCTTATTTATCAATGATTAC
TGGCAGTTGGCGGTTAAACACGGCGCTTATGGCGTTCATCTCGGTCAGGAAGATCTGGAAACCGCTGATTTAGCGGCAAT
TCAGCAAGCGGGGTTACGGCTCGGCATTTCTACCCATGATGAACATGAGTTGGCGGTGGCAAAAACACTGCGCCCCTCTT
ATATCGCGCTGGGGCATATTTTCCCTACTCAGACCAAACAGATGCCGTCATCCCCACAGGGGTTGGCGTCTTTAAGCCGT
CAGGTGAAGAATACGCCGGATTACCCAACCGTGGCTATCGGTGGCATCAGTATTGAGCGGGTGCCACACGTATTAGCAAC
TGGCGTCGGCAGTGTCGCCGTGGTCAGCGCCATTACGTTGGCAAGTGACTGGCAACGGGCAACCGCACAATTACTGCACC
TGATCGAGGGTAAGGAGCTGGCTGATGAAAAATGA

Upstream 100 bases:

>100_bases
AATATGCTGCAATCAGGGATGGAAAAAATGTCGGCCGAGTTTCGCTCCCGTGGCAGTGAGCTATACCACCGCCCGGCGAA
TCTGAGTGCGGAGGCCAATA

Downstream 100 bases:

>100_bases
CCAGACGCTAAGTGACAGTGAATTTTTACGCTATAGCCGCCAACTGCTACTGGAAGATATCGGCCCGGAAGGGCAACGAA
AGCTAAAGGGTGCCAGTGTG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MSQLDALSPVATPGFPSTEQRLGLYPVVDSLLWIERLLAAGVTTLQLRIKNADDAQVEQDIVAAIELGKRYQARLFINDY
WQLAVKHGAYGVHLGQEDLETADLAAIQQAGLRLGISTHDEHELAVAKTLRPSYIALGHIFPTQTKQMPSSPQGLASLSR
QVKNTPDYPTVAIGGISIERVPHVLATGVGSVAVVSAITLASDWQRATAQLLHLIEGKELADEK

Sequences:

>Translated_224_residues
MSQLDALSPVATPGFPSTEQRLGLYPVVDSLLWIERLLAAGVTTLQLRIKNADDAQVEQDIVAAIELGKRYQARLFINDY
WQLAVKHGAYGVHLGQEDLETADLAAIQQAGLRLGISTHDEHELAVAKTLRPSYIALGHIFPTQTKQMPSSPQGLASLSR
QVKNTPDYPTVAIGGISIERVPHVLATGVGSVAVVSAITLASDWQRATAQLLHLIEGKELADEK
>Mature_223_residues
SQLDALSPVATPGFPSTEQRLGLYPVVDSLLWIERLLAAGVTTLQLRIKNADDAQVEQDIVAAIELGKRYQARLFINDYW
QLAVKHGAYGVHLGQEDLETADLAAIQQAGLRLGISTHDEHELAVAKTLRPSYIALGHIFPTQTKQMPSSPQGLASLSRQ
VKNTPDYPTVAIGGISIERVPHVLATGVGSVAVVSAITLASDWQRATAQLLHLIEGKELADEK

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=202, Percent_Identity=70.7920792079208, Blast_Score=293, Evalue=8e-81,
Organism=Saccharomyces cerevisiae, GI6325042, Length=228, Percent_Identity=28.0701754385965, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 24216; Mature: 24085

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLDALSPVATPGFPSTEQRLGLYPVVDSLLWIERLLAAGVTTLQLRIKNADDAQVEQD
CCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHH
IVAAIELGKRYQARLFINDYWQLAVKHGAYGVHLGQEDLETADLAAIQQAGLRLGISTHD
HHHHHHHCCHHEEEEEEHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHCCCEECCCCCC
EHELAVAKTLRPSYIALGHIFPTQTKQMPSSPQGLASLSRQVKNTPDYPTVAIGGISIER
CHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCHHH
VPHVLATGVGSVAVVSAITLASDWQRATAQLLHLIEGKELADEK
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure 
SQLDALSPVATPGFPSTEQRLGLYPVVDSLLWIERLLAAGVTTLQLRIKNADDAQVEQD
CCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHH
IVAAIELGKRYQARLFINDYWQLAVKHGAYGVHLGQEDLETADLAAIQQAGLRLGISTHD
HHHHHHHCCHHEEEEEEHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHCCCEECCCCCC
EHELAVAKTLRPSYIALGHIFPTQTKQMPSSPQGLASLSRQVKNTPDYPTVAIGGISIER
CHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCHHH
VPHVLATGVGSVAVVSAITLASDWQRATAQLLHLIEGKELADEK
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA