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The map label for this gene is mscL

Identifier: 170022573

GI number: 170022573

Start: 334294

End: 334698

Strand: Reverse

Name: mscL

Synonym: YPK_0312

Alternate gene names: 170022573

Gene position: 334698-334294 (Counterclockwise)

Preceding gene: 170022574

Following gene: 170022530

Centisome position: 7.14

GC content: 42.22

Gene sequence:

>405_bases
ATGAAAGAATTTCGTGAATTTGCCATGCGTGGCAATGTGGTTGACTTAGCTGTTGGTGTGATTATTGGTGCTGCATTCGG
CAGAATTGTCTCTTCGCTCGTTGCCGATATTATCATGCCACCACTGGGGTTATTGCTAGGCGGGGTAGATTTTAAACAAT
TCCATTTTGTATTACGAGCCGCTGAAGGGACTATACCCGCGGTAGTAATGAATTATGGTACATTTATTCAAAGCATTTTT
GATTTTGTTATTGTAGCCCTCGCTATATTCTCCGCTGTTAAGTTAATGAATAAGCTACGCCGTGAAAAAGCGGAAGAAGA
ACCTGCTACACCGCCAGCACCAACGACTGAAGAGATACTATTAGCTGAGATTCGCGATTTATTAAAAGCGCAGCACACAA
AATAG

Upstream 100 bases:

>100_bases
AGGAAATAATTAAAATTTCCCTATAATTTTAGTGATAATAGGCATTATTTAGCTAGAATTAAGTCATTCATTTATTGATG
GGGTGTTGGCTATGAGCTTT

Downstream 100 bases:

>100_bases
TTTCAGTTTAGCTAAATTTAAGAGCTACCGGAATGAGTTGGCCTAAACAGCAACACAAAAGCGGGTATTTGGATTAAGTC
CAAGAAGATAACTTTTTATT

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 134; Mature: 134

Protein sequence:

>134_residues
MKEFREFAMRGNVVDLAVGVIIGAAFGRIVSSLVADIIMPPLGLLLGGVDFKQFHFVLRAAEGTIPAVVMNYGTFIQSIF
DFVIVALAIFSAVKLMNKLRREKAEEEPATPPAPTTEEILLAEIRDLLKAQHTK

Sequences:

>Translated_134_residues
MKEFREFAMRGNVVDLAVGVIIGAAFGRIVSSLVADIIMPPLGLLLGGVDFKQFHFVLRAAEGTIPAVVMNYGTFIQSIF
DFVIVALAIFSAVKLMNKLRREKAEEEPATPPAPTTEEILLAEIRDLLKAQHTK
>Mature_134_residues
MKEFREFAMRGNVVDLAVGVIIGAAFGRIVSSLVADIIMPPLGLLLGGVDFKQFHFVLRAAEGTIPAVVMNYGTFIQSIF
DFVIVALAIFSAVKLMNKLRREKAEEEPATPPAPTTEEILLAEIRDLLKAQHTK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=134, Percent_Identity=76.1194029850746, Blast_Score=206, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_YERP3 (A7FNK6)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001402834.1
- ProteinModelPortal:   A7FNK6
- SMR:   A7FNK6
- STRING:   A7FNK6
- GeneID:   5388100
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_3886
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- ProtClustDB:   PRK00567
- BioCyc:   YPSE349747:YPSIP31758_3886-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 14684; Mature: 14684

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x15063b04)-; HASH(0x1478536c)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEFREFAMRGNVVDLAVGVIIGAAFGRIVSSLVADIIMPPLGLLLGGVDFKQFHFVLRA
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AEGTIPAVVMNYGTFIQSIFDFVIVALAIFSAVKLMNKLRREKAEEEPATPPAPTTEEIL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
LAEIRDLLKAQHTK
HHHHHHHHHHHCCH
>Mature Secondary Structure
MKEFREFAMRGNVVDLAVGVIIGAAFGRIVSSLVADIIMPPLGLLLGGVDFKQFHFVLRA
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AEGTIPAVVMNYGTFIQSIFDFVIVALAIFSAVKLMNKLRREKAEEEPATPPAPTTEEIL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
LAEIRDLLKAQHTK
HHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA