Click here to switch to the map view.

The map label for this gene is gph

Identifier: 170022491

GI number: 170022491

Start: 268234

End: 268932

Strand: Direct

Name: gph

Synonym: YPK_0230

Alternate gene names: 170022491

Gene position: 268234-268932 (Clockwise)

Preceding gene: 170022490

Following gene: 170022492

Centisome position: 5.72

GC content: 51.5

Gene sequence:

>699_bases
ATGGTTAAGTTTAAAGCGATTCGTGGTGTTGCATTCGATCTGGATGGCACATTAGTCGACAGCGCGCCTGGTCTGGCCCG
TGCAATTGATATGGCGTTGGCGCATCAGGGGTTGCCTGCCGCTGGTGAAGCGTTAGTCTCCACTTGGATTGGTAATGGCG
CAGATGTCTTAGTCGAGCGCGCGTTGCATTGGGCAGGGCGTGAACATAATGCTCAGCTTGTGGCACAGACCCGCGAGCTG
TTTGATCACTATTATGCCAAAACCGTTGAGCAGGGGAGCCAGTTATTCCCGCAAGTGAAAGCCACTTTAGCTCAATTGGC
TGCCAATGGTTTGCCAATAGGCCTCATTACCAATAAGCCAACACCGTTTGTTGCCCCTTTACTAACGTCATTGGGGATCT
CAGACTATTTCTCAGTCATCATTGGGGGCGATGATGTGGTGGTGAAGAAACCGCATCCGGCCCCGTTGTATTTATTGCTG
GGAAAACTTGGCTTACATGCCCGTGAAATGCTGTTTGTCGGTGACTCGCGTAATGACATTATGGCCGCACAGGCGGCGGG
TTGCCCTTGTATTGGGCTGACCTATGGATATAACTACGGTGAAGCGATTGCCACCAGTCATCCAGACTGCGTGCTGGCGC
ATTTTGCCGACCTGTTGCCCGCCATTGGGCTACCATCTTTAAAAGATCAGGAAGTATAA

Upstream 100 bases:

>100_bases
GCAGGTGCCGATATGTTTGTTGCTGGCTCCGCGATTTTCAATCAACCCGATTATGCTGCGGTGATCGATGCCATGCGCAA
TGAACTGGCGATGGCGGCTA

Downstream 100 bases:

>100_bases
AATGAGTGAACCCATGGTATTAAGCAAACCCACTGTATCCAGTAAACCTATCGTATTCAGCGGCGCACAGCCGTCCGGCG
AATTGACCATTGGTAATTAC

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MVKFKAIRGVAFDLDGTLVDSAPGLARAIDMALAHQGLPAAGEALVSTWIGNGADVLVERALHWAGREHNAQLVAQTREL
FDHYYAKTVEQGSQLFPQVKATLAQLAANGLPIGLITNKPTPFVAPLLTSLGISDYFSVIIGGDDVVVKKPHPAPLYLLL
GKLGLHAREMLFVGDSRNDIMAAQAAGCPCIGLTYGYNYGEAIATSHPDCVLAHFADLLPAIGLPSLKDQEV

Sequences:

>Translated_232_residues
MVKFKAIRGVAFDLDGTLVDSAPGLARAIDMALAHQGLPAAGEALVSTWIGNGADVLVERALHWAGREHNAQLVAQTREL
FDHYYAKTVEQGSQLFPQVKATLAQLAANGLPIGLITNKPTPFVAPLLTSLGISDYFSVIIGGDDVVVKKPHPAPLYLLL
GKLGLHAREMLFVGDSRNDIMAAQAAGCPCIGLTYGYNYGEAIATSHPDCVLAHFADLLPAIGLPSLKDQEV
>Mature_232_residues
MVKFKAIRGVAFDLDGTLVDSAPGLARAIDMALAHQGLPAAGEALVSTWIGNGADVLVERALHWAGREHNAQLVAQTREL
FDHYYAKTVEQGSQLFPQVKATLAQLAANGLPIGLITNKPTPFVAPLLTSLGISDYFSVIIGGDDVVVKKPHPAPLYLLL
GKLGLHAREMLFVGDSRNDIMAAQAAGCPCIGLTYGYNYGEAIATSHPDCVLAHFADLLPAIGLPSLKDQEV

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family

Homologues:

Organism=Escherichia coli, GI1789787, Length=248, Percent_Identity=60.4838709677419, Blast_Score=293, Evalue=5e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GPH_YERPE (Q8ZJF3)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AI0019
- RefSeq:   YP_002345242.1
- ProteinModelPortal:   Q8ZJF3
- SMR:   Q8ZJF3
- IntAct:   Q8ZJF3
- GeneID:   1173002
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO0156
- KEGG:   ypk:y3939
- KEGG:   ypm:YP_0158
- HOGENOM:   HBG742904
- OMA:   YNYGESI
- ProtClustDB:   PRK13222
- BioCyc:   YPES187410:Y3939-MONOMER
- BioCyc:   YPES214092:YPO0156-MONOMER
- BRENDA:   3.1.3.18
- HAMAP:   MF_00495
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198
- Gene3D:   G3DSA:3.40.50.1000
- Gene3D:   G3DSA:1.10.150.240
- PRINTS:   PR00413
- TIGRFAMs:   TIGR01549
- TIGRFAMs:   TIGR01509
- TIGRFAMs:   TIGR01449

Pfam domain/function: PF00702 Hydrolase; SSF56784 SSF56784

EC number: =3.1.3.18

Molecular weight: Translated: 24606; Mature: 24606

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: ACT_SITE 13-13

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKFKAIRGVAFDLDGTLVDSAPGLARAIDMALAHQGLPAAGEALVSTWIGNGADVLVER
CCEEEECCCEEEECCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHH
ALHWAGREHNAQLVAQTRELFDHYYAKTVEQGSQLFPQVKATLAQLAANGLPIGLITNKP
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
TPFVAPLLTSLGISDYFSVIIGGDDVVVKKPHPAPLYLLLGKLGLHAREMLFVGDSRNDI
CCHHHHHHHHCCHHHHHEEEECCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCE
MAAQAAGCPCIGLTYGYNYGEAIATSHPDCVLAHFADLLPAIGLPSLKDQEV
EEHHCCCCCEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MVKFKAIRGVAFDLDGTLVDSAPGLARAIDMALAHQGLPAAGEALVSTWIGNGADVLVER
CCEEEECCCEEEECCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHH
ALHWAGREHNAQLVAQTRELFDHYYAKTVEQGSQLFPQVKATLAQLAANGLPIGLITNKP
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
TPFVAPLLTSLGISDYFSVIIGGDDVVVKKPHPAPLYLLLGKLGLHAREMLFVGDSRNDI
CCHHHHHHHHCCHHHHHEEEECCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCE
MAAQAAGCPCIGLTYGYNYGEAIATSHPDCVLAHFADLLPAIGLPSLKDQEV
EEHHCCCCCEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430