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The map label for this gene is dpnM [H]

Identifier: 170022442

GI number: 170022442

Start: 233187

End: 233942

Strand: Reverse

Name: dpnM [H]

Synonym: YPK_0181

Alternate gene names: 170022442

Gene position: 233942-233187 (Counterclockwise)

Preceding gene: 170022443

Following gene: 170022439

Centisome position: 4.99

GC content: 45.37

Gene sequence:

>756_bases
ATGAGCACCCCAATAATTCCCTGGGTTGGTGGTAAACGACGGTTGGCAAAACATATTTTGCCGCTGTTCCCTGCACATAC
ATGCTATATCGAGCCGTTTTGTGGGGGTGCAGCTCTATATTTTCTCAAAGAGACTTCTAAAGTAGAGGTTATTAATGATG
TTAATGGTGAGCTAATTAACCTTTATCGCGTCATTAAAAACCATTTAGAAGAGTTTGTAAGGCAGTTTAAATGGGCATTA
ACCAGCCGGCAAGTATTTGAGTGGACGAAAGCCACGCCGACAGAGGTGCTCACTGACATACAGAGGGCGGCTCGGTTTTA
CTATCTGCAAACGACAGCCTTTGGCGGTAAGGTGGATGGTCAGACATTTGGCACATCAACTACAAGACCAACAGGACTGA
ACTTGTTGCGCCTTGAAGAAACGCTATCAGCGGCACACCTGAGGCTATCCCGCACAATGATAGAACACATGGGGTGGGCT
GATTGTGTAGAAAAATATGACCGTCCACATTCATTATTTTACTTAGATCCACCTTACTGGCAGACCTGTGGCTACGGGGT
TGAGTTCGGACTTGATCAATATACCCAAATGGCCCAGTTAGCGCGTTCTATTAAGGGCAGTATGATAATCTCAGTCAACG
ATATCCCGGAGATGCGGGACGTCTTTAAGGGACTGCATATGGATACTGTGAGCCTTAAATACTCATTAGGGGACAACAAA
AAGCAGGTGAGCGAGTTAATCATCCGAAATTTCTAG

Upstream 100 bases:

>100_bases
ATATGCGGCTTTTTTAGTACCCAACCCCACTTGATCGATTTAACCGATCAAATATTAATTACTGATCAGTTAAATCGATC
ATTAAGAAGGCATACATTTT

Downstream 100 bases:

>100_bases
CGGCCTATTTTGCATTGTGCAGAATAGACAATTATAGCGCGGAGGCATTATGCAAAAATGGTCGTTAATCTATGCAAAAA
ATTTCGCCGCGCTACACACG

Product: D12 class N6 adenine-specific DNA methyltransferase

Products: NA

Alternate protein names: M.DpnIIA; Adenine-specific methyltransferase DpnIIA; M.DpnII 1 [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MSTPIIPWVGGKRRLAKHILPLFPAHTCYIEPFCGGAALYFLKETSKVEVINDVNGELINLYRVIKNHLEEFVRQFKWAL
TSRQVFEWTKATPTEVLTDIQRAARFYYLQTTAFGGKVDGQTFGTSTTRPTGLNLLRLEETLSAAHLRLSRTMIEHMGWA
DCVEKYDRPHSLFYLDPPYWQTCGYGVEFGLDQYTQMAQLARSIKGSMIISVNDIPEMRDVFKGLHMDTVSLKYSLGDNK
KQVSELIIRNF

Sequences:

>Translated_251_residues
MSTPIIPWVGGKRRLAKHILPLFPAHTCYIEPFCGGAALYFLKETSKVEVINDVNGELINLYRVIKNHLEEFVRQFKWAL
TSRQVFEWTKATPTEVLTDIQRAARFYYLQTTAFGGKVDGQTFGTSTTRPTGLNLLRLEETLSAAHLRLSRTMIEHMGWA
DCVEKYDRPHSLFYLDPPYWQTCGYGVEFGLDQYTQMAQLARSIKGSMIISVNDIPEMRDVFKGLHMDTVSLKYSLGDNK
KQVSELIIRNF
>Mature_250_residues
STPIIPWVGGKRRLAKHILPLFPAHTCYIEPFCGGAALYFLKETSKVEVINDVNGELINLYRVIKNHLEEFVRQFKWALT
SRQVFEWTKATPTEVLTDIQRAARFYYLQTTAFGGKVDGQTFGTSTTRPTGLNLLRLEETLSAAHLRLSRTMIEHMGWAD
CVEKYDRPHSLFYLDPPYWQTCGYGVEFGLDQYTQMAQLARSIKGSMIISVNDIPEMRDVFKGLHMDTVSLKYSLGDNKK
QVSELIIRNF

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the DpnII endonuclease [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 28797; Mature: 28665

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPIIPWVGGKRRLAKHILPLFPAHTCYIEPFCGGAALYFLKETSKVEVINDVNGELIN
CCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCEEEEECCCHHHHH
LYRVIKNHLEEFVRQFKWALTSRQVFEWTKATPTEVLTDIQRAARFYYLQTTAFGGKVDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEECCCCCCCC
QTFGTSTTRPTGLNLLRLEETLSAAHLRLSRTMIEHMGWADCVEKYDRPHSLFYLDPPYW
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCH
QTCGYGVEFGLDQYTQMAQLARSIKGSMIISVNDIPEMRDVFKGLHMDTVSLKYSLGDNK
HHCCCCHHCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCEEEEEEECCCCH
KQVSELIIRNF
HHHHHHHHHCC
>Mature Secondary Structure 
STPIIPWVGGKRRLAKHILPLFPAHTCYIEPFCGGAALYFLKETSKVEVINDVNGELIN
CCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCEEEEECCCHHHHH
LYRVIKNHLEEFVRQFKWALTSRQVFEWTKATPTEVLTDIQRAARFYYLQTTAFGGKVDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEECCCCCCCC
QTFGTSTTRPTGLNLLRLEETLSAAHLRLSRTMIEHMGWADCVEKYDRPHSLFYLDPPYW
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCH
QTCGYGVEFGLDQYTQMAQLARSIKGSMIISVNDIPEMRDVFKGLHMDTVSLKYSLGDNK
HHCCCCHHCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCEEEEEEECCCCH
KQVSELIIRNF
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2989823; 3019562; 2824782; 9862809 [H]