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The map label for this gene is pitA [H]

Identifier: 170022383

GI number: 170022383

Start: 146641

End: 148236

Strand: Reverse

Name: pitA [H]

Synonym: YPK_0122

Alternate gene names: 170022383

Gene position: 148236-146641 (Counterclockwise)

Preceding gene: 170022385

Following gene: 170022381

Centisome position: 3.16

GC content: 50.0

Gene sequence:

>1596_bases
ATGATGTTATGTCAAAAAAAGGTCATATTTTCCTTTTACCAACCCCCCATTGTCACTGATAATGCGCCGCGTTCATGTCC
ACAAACTGGCGTAACGCTTATGCTACATCTTTTCGCTGGCCTGGATTTCCATACCGGCCTTATGTTAGTTCTTGCTTTGT
TGTTCGTGCTGTTTTACGAAGCCATCAATGGTTTTCATGATACAGCCAATGCGGTTGCGACCGTAATCTATACCCGTGCC
ATGCGTTCACAGATCGCAGTTGTGATGGCAGGGGTGTTTAACTTCCTCGGCGTGATGCTGGGTGGTTTGAGCGTGGCCTA
CGCCATTGTTCACTTACTGCCTACTGATCTGCTGTTAAACGTCAGTTCCACGCATGGGTTGGCAATGGTCTTCTCCATGT
TACTGGCCGCGATTATCTGGAATTTGGGTACCTGGTATTTTGGTATTCCAGCCTCCAGTTCTCATACGCTGATTGGTGCT
ATCATCGGTATTGGTCTAACCAATGCATTAATGACCAGTACATCCGTTGTGGATGCGCTGAACGTTCCTAAGATGATACA
AATCTTTCTGTCGCTGATCTTATCCCCGATTGTGGGTCTGGTTATCGCCGGTTTGATGGTATTCCTGCTACGTCGTTACT
GGAGCAGTACCAAAAAGCAGCAACGTATTCACCTGACGCCTGCTGAACGTGAAAAGAAAGACGGTAAACGTAAGCCGCCA
TTCTGGACCCGCACGGCGCTGATCCTCTCCGCTATCGGTGTGAGTTTCTCTCACGGCGCTAACGATGGTCAGAAAGGGAT
TGGCTTGATCATGCTGGTATTAATCGGTGTGGCTCCGGCTGGTTTTGTCGTGAATATGAATGCGACTGGCTATGATATTG
CCCGCACCCGCGATGCAGTCACTCATGTGCAACTCTATTATCAGCAACATGCTGAGGCATTGCCTCATGCCATCGCGCTG
AAACCACTGGTACCAGCCCCAGAAACCTTGCCAGGTACACCTGCGCAATTCCACTGTGACACTTCACGCGCTATGGTCGC
CATTAACCATGCACAAGCGCTGCTGACCGATCTGAAAAGCTATGATGACCTAACCGTTGATCAACGCAGCCAAATGCGTC
GCTTGCTGATGTGTGTCGCAGAAACCGCAGGGGCCGTTGCCAAACTGCCAGAAACCAGTGCTGAAAACCGCCGTTTCCTC
AACAATCTGCGTACCGATTTGTTGGACACAGTGGAATATGCACCAACCTGGATTATCGTAGCGGTTGCTCTGGCTCTGTC
TCTGGGGACGATGATCGGTTGGAGACGTGTTGCCGTCACTATCGGTGAGAAGATCGGCAAAAAAGGCATGACCTATGCTC
AAGGGGTTTCAGCCCAGATGACTGCGGCGGTCTCTATCGGTATTGCCAGTTATACCGGCATGCCAGTATCGACCACTCAA
GTGCTCTCTTCTGCTGTTGCCGGGACCATGTTGGTCGATGGCGGTGGCGTGCAGAGTAAAACGGTGAAGAACATTATGTT
GGCCTGGGTCTTAACCCTGCCAATCTCTATCTTCCTTTCCGGTGCACTGTATTGGATAGCCTTAAAGTTCATTTAA

Upstream 100 bases:

>100_bases
CCCTGCTGGCAGAGCGCCATAGTAAGAAAATGTTATATTCGGTAAAACTAAGTAACTCGCTGATATAACATACATATGTG
GTGCAAAAAGACTAAGCCAT

Downstream 100 bases:

>100_bases
GTTAATTCAACGCTAAGCTCATTCGACGGTAAGCCAATTCGACGAATTGTTATCACCGAGAGCCTTAGCGCGATGTCAGT
GATGAGCAATAAAAAGGCGG

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MMLCQKKVIFSFYQPPIVTDNAPRSCPQTGVTLMLHLFAGLDFHTGLMLVLALLFVLFYEAINGFHDTANAVATVIYTRA
MRSQIAVVMAGVFNFLGVMLGGLSVAYAIVHLLPTDLLLNVSSTHGLAMVFSMLLAAIIWNLGTWYFGIPASSSHTLIGA
IIGIGLTNALMTSTSVVDALNVPKMIQIFLSLILSPIVGLVIAGLMVFLLRRYWSSTKKQQRIHLTPAEREKKDGKRKPP
FWTRTALILSAIGVSFSHGANDGQKGIGLIMLVLIGVAPAGFVVNMNATGYDIARTRDAVTHVQLYYQQHAEALPHAIAL
KPLVPAPETLPGTPAQFHCDTSRAMVAINHAQALLTDLKSYDDLTVDQRSQMRRLLMCVAETAGAVAKLPETSAENRRFL
NNLRTDLLDTVEYAPTWIIVAVALALSLGTMIGWRRVAVTIGEKIGKKGMTYAQGVSAQMTAAVSIGIASYTGMPVSTTQ
VLSSAVAGTMLVDGGGVQSKTVKNIMLAWVLTLPISIFLSGALYWIALKFI

Sequences:

>Translated_531_residues
MMLCQKKVIFSFYQPPIVTDNAPRSCPQTGVTLMLHLFAGLDFHTGLMLVLALLFVLFYEAINGFHDTANAVATVIYTRA
MRSQIAVVMAGVFNFLGVMLGGLSVAYAIVHLLPTDLLLNVSSTHGLAMVFSMLLAAIIWNLGTWYFGIPASSSHTLIGA
IIGIGLTNALMTSTSVVDALNVPKMIQIFLSLILSPIVGLVIAGLMVFLLRRYWSSTKKQQRIHLTPAEREKKDGKRKPP
FWTRTALILSAIGVSFSHGANDGQKGIGLIMLVLIGVAPAGFVVNMNATGYDIARTRDAVTHVQLYYQQHAEALPHAIAL
KPLVPAPETLPGTPAQFHCDTSRAMVAINHAQALLTDLKSYDDLTVDQRSQMRRLLMCVAETAGAVAKLPETSAENRRFL
NNLRTDLLDTVEYAPTWIIVAVALALSLGTMIGWRRVAVTIGEKIGKKGMTYAQGVSAQMTAAVSIGIASYTGMPVSTTQ
VLSSAVAGTMLVDGGGVQSKTVKNIMLAWVLTLPISIFLSGALYWIALKFI
>Mature_531_residues
MMLCQKKVIFSFYQPPIVTDNAPRSCPQTGVTLMLHLFAGLDFHTGLMLVLALLFVLFYEAINGFHDTANAVATVIYTRA
MRSQIAVVMAGVFNFLGVMLGGLSVAYAIVHLLPTDLLLNVSSTHGLAMVFSMLLAAIIWNLGTWYFGIPASSSHTLIGA
IIGIGLTNALMTSTSVVDALNVPKMIQIFLSLILSPIVGLVIAGLMVFLLRRYWSSTKKQQRIHLTPAEREKKDGKRKPP
FWTRTALILSAIGVSFSHGANDGQKGIGLIMLVLIGVAPAGFVVNMNATGYDIARTRDAVTHVQLYYQQHAEALPHAIAL
KPLVPAPETLPGTPAQFHCDTSRAMVAINHAQALLTDLKSYDDLTVDQRSQMRRLLMCVAETAGAVAKLPETSAENRRFL
NNLRTDLLDTVEYAPTWIIVAVALALSLGTMIGWRRVAVTIGEKIGKKGMTYAQGVSAQMTAAVSIGIASYTGMPVSTTQ
VLSSAVAGTMLVDGGGVQSKTVKNIMLAWVLTLPISIFLSGALYWIALKFI

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=499, Percent_Identity=75.5511022044088, Blast_Score=757, Evalue=0.0,
Organism=Escherichia coli, GI1789360, Length=499, Percent_Identity=70.3406813627254, Blast_Score=686, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25146401, Length=509, Percent_Identity=22.2003929273084, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 57302; Mature: 57302

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLCQKKVIFSFYQPPIVTDNAPRSCPQTGVTLMLHLFAGLDFHTGLMLVLALLFVLFYE
CCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AINGFHDTANAVATVIYTRAMRSQIAVVMAGVFNFLGVMLGGLSVAYAIVHLLPTDLLLN
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
VSSTHGLAMVFSMLLAAIIWNLGTWYFGIPASSSHTLIGAIIGIGLTNALMTSTSVVDAL
CCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NVPKMIQIFLSLILSPIVGLVIAGLMVFLLRRYWSSTKKQQRIHLTPAEREKKDGKRKPP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHCCCCCCC
FWTRTALILSAIGVSFSHGANDGQKGIGLIMLVLIGVAPAGFVVNMNATGYDIARTRDAV
CHHHHHHHHHHHHCHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHH
THVQLYYQQHAEALPHAIALKPLVPAPETLPGTPAQFHCDTSRAMVAINHAQALLTDLKS
HHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHH
YDDLTVDQRSQMRRLLMCVAETAGAVAKLPETSAENRRFLNNLRTDLLDTVEYAPTWIIV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVALALSLGTMIGWRRVAVTIGEKIGKKGMTYAQGVSAQMTAAVSIGIASYTGMPVSTTQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCHHHH
VLSSAVAGTMLVDGGGVQSKTVKNIMLAWVLTLPISIFLSGALYWIALKFI
HHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMLCQKKVIFSFYQPPIVTDNAPRSCPQTGVTLMLHLFAGLDFHTGLMLVLALLFVLFYE
CCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AINGFHDTANAVATVIYTRAMRSQIAVVMAGVFNFLGVMLGGLSVAYAIVHLLPTDLLLN
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
VSSTHGLAMVFSMLLAAIIWNLGTWYFGIPASSSHTLIGAIIGIGLTNALMTSTSVVDAL
CCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NVPKMIQIFLSLILSPIVGLVIAGLMVFLLRRYWSSTKKQQRIHLTPAEREKKDGKRKPP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHCCCCCCC
FWTRTALILSAIGVSFSHGANDGQKGIGLIMLVLIGVAPAGFVVNMNATGYDIARTRDAV
CHHHHHHHHHHHHCHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHH
THVQLYYQQHAEALPHAIALKPLVPAPETLPGTPAQFHCDTSRAMVAINHAQALLTDLKS
HHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHH
YDDLTVDQRSQMRRLLMCVAETAGAVAKLPETSAENRRFLNNLRTDLLDTVEYAPTWIIV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVALALSLGTMIGWRRVAVTIGEKIGKKGMTYAQGVSAQMTAAVSIGIASYTGMPVSTTQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCHHHH
VLSSAVAGTMLVDGGGVQSKTVKNIMLAWVLTLPISIFLSGALYWIALKFI
HHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]