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The map label for this gene is cmr [H]

Identifier: 170022310

GI number: 170022310

Start: 51682

End: 52911

Strand: Reverse

Name: cmr [H]

Synonym: YPK_0048

Alternate gene names: 170022310

Gene position: 52911-51682 (Counterclockwise)

Preceding gene: 170022317

Following gene: 170022308

Centisome position: 1.13

GC content: 51.63

Gene sequence:

>1230_bases
ATGCAAACTTCATTTTCACCCGCGACACGCTTAGGTCGACGGGCGCTTTTATTCCCTCTCTGCTTAGTATTGTTCGAATT
TGCAGCGTATATCGCCAACGATATGATCCAGCCAGGTATGCTGGCAGTGGTCGCCGAGTTTAATGCCAGTGTCGAGTGGG
TTCCAACCTCTATGACCGCTTATCTGGCGGGGGGGATGTTCCTACAATGGCTTCTAGGGCCGTTGTCTGACCGCCGTGGG
CGTCGTCCAGTGATGTTGGCTGGGGTCGCTTTCTTTGTCGTGACTTGCCTTGCCATTTTACTGGTTAATAGCATTGAACA
GTTCATCGCAATGCGCTTCTTGCAAGGCATTGGCTTGTGCTTTATCGGTGCCGTCGGTTACGCCACTATTCAGGAATCTT
TTGAAGAAGCGGTCTGTATTAAAATCACCGCGTTAATGGCCAATGTGGCATTGATTGCTCCGTTGCTTGGCCCATTGGCC
GGTGCTGCCCTGATCCATGTGGCTCCTTGGCAAACCATGTTCGTCTTGTTTGCAGTGCTGGGGGCAATTTCATTTGCCGG
CCTGTGGCGCGCGATGCCAGAAACTGCTTCGCTGAAAGGGGAGAAACTGTCAGTTGCCAACATGTGGCATGATTATAAGC
AAGTACTTGCTAACCGCCGCTTCCTCTGTGGTTCTTTAGCATTGGGCTTTGCCAGCCTGCCTTTGCTGGCATGGATTGCC
CAGTCACCGGTAATTTTGATCAGCGGAGAGCAACTCTCTACTTTTGAATATGGCATTTTGCAGGTACCAATTTTCGGCGC
ACTGATTATAGGTAACCTGACACTGGCACGATTGAGCGGTAAAACCAGCATTCCTCAACTCATCCGTTACGGCGCTGGCC
CAATGATTGTTGGCCTGATGATCGCCGCCGGTTCAACACTCTATTCATCCCATGCCTATCTGTGGATGACCGCCGGGTTG
AGCCTGTATGCTTTTGGTATCGGTCTGGCGAACGCCGGTCTGGTGCGGTTGACACTATTCGCCAGTGATATCAGTAAAGG
AACCGTCTCTGCGGCCATGGGGATGATTAGCATGATGATCTTCACCTTAGGTATTGAGTTGGCCAAAGTGGCTTACCTGT
GGGGCGACAGTAGAGGGTTTAATCTGTTTAACCTGATGAGCGGTTTACTGTGGCTAGGCTTGGTGATGGTGTTCATCCGC
CGCCAACCCGAGGCGGTTGCTACCGAGTAA

Upstream 100 bases:

>100_bases
ACACGCTATTGACGGCAAAATAACCGCTCTGACATGTTCCGACTGTAGGCCCTGCCCAAGCAGTTCCCTTTTACATACTT
ATTATTAATTAGCTTAATTT

Downstream 100 bases:

>100_bases
GTTTATTCCCTGCTGGAGGAGCGTCAGGTAGCTTCTCCAGCAGGATAGTTTTACCCACGCTATATTTCTCAAGACGGTAT
TAAAAACACACCGATACGTA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Chloramphenicol resistance pump cmr [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MQTSFSPATRLGRRALLFPLCLVLFEFAAYIANDMIQPGMLAVVAEFNASVEWVPTSMTAYLAGGMFLQWLLGPLSDRRG
RRPVMLAGVAFFVVTCLAILLVNSIEQFIAMRFLQGIGLCFIGAVGYATIQESFEEAVCIKITALMANVALIAPLLGPLA
GAALIHVAPWQTMFVLFAVLGAISFAGLWRAMPETASLKGEKLSVANMWHDYKQVLANRRFLCGSLALGFASLPLLAWIA
QSPVILISGEQLSTFEYGILQVPIFGALIIGNLTLARLSGKTSIPQLIRYGAGPMIVGLMIAAGSTLYSSHAYLWMTAGL
SLYAFGIGLANAGLVRLTLFASDISKGTVSAAMGMISMMIFTLGIELAKVAYLWGDSRGFNLFNLMSGLLWLGLVMVFIR
RQPEAVATE

Sequences:

>Translated_409_residues
MQTSFSPATRLGRRALLFPLCLVLFEFAAYIANDMIQPGMLAVVAEFNASVEWVPTSMTAYLAGGMFLQWLLGPLSDRRG
RRPVMLAGVAFFVVTCLAILLVNSIEQFIAMRFLQGIGLCFIGAVGYATIQESFEEAVCIKITALMANVALIAPLLGPLA
GAALIHVAPWQTMFVLFAVLGAISFAGLWRAMPETASLKGEKLSVANMWHDYKQVLANRRFLCGSLALGFASLPLLAWIA
QSPVILISGEQLSTFEYGILQVPIFGALIIGNLTLARLSGKTSIPQLIRYGAGPMIVGLMIAAGSTLYSSHAYLWMTAGL
SLYAFGIGLANAGLVRLTLFASDISKGTVSAAMGMISMMIFTLGIELAKVAYLWGDSRGFNLFNLMSGLLWLGLVMVFIR
RQPEAVATE
>Mature_409_residues
MQTSFSPATRLGRRALLFPLCLVLFEFAAYIANDMIQPGMLAVVAEFNASVEWVPTSMTAYLAGGMFLQWLLGPLSDRRG
RRPVMLAGVAFFVVTCLAILLVNSIEQFIAMRFLQGIGLCFIGAVGYATIQESFEEAVCIKITALMANVALIAPLLGPLA
GAALIHVAPWQTMFVLFAVLGAISFAGLWRAMPETASLKGEKLSVANMWHDYKQVLANRRFLCGSLALGFASLPLLAWIA
QSPVILISGEQLSTFEYGILQVPIFGALIIGNLTLARLSGKTSIPQLIRYGAGPMIVGLMIAAGSTLYSSHAYLWMTAGL
SLYAFGIGLANAGLVRLTLFASDISKGTVSAAMGMISMMIFTLGIELAKVAYLWGDSRGFNLFNLMSGLLWLGLVMVFIR
RQPEAVATE

Specific function: Efflux pump driven by the proton motive force. Confers resistance to basic, aromatic and lipophilic drugs as well as neutral, non-aromatic, zwitterionic or non-aromatic hydrophilic antibiotics [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787065, Length=409, Percent_Identity=71.3936430317848, Blast_Score=587, Evalue=1e-169,
Organism=Escherichia coli, GI1790794, Length=375, Percent_Identity=42.6666666666667, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI48994889, Length=264, Percent_Identity=28.030303030303, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790146, Length=228, Percent_Identity=27.6315789473684, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1788509, Length=258, Percent_Identity=23.2558139534884, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87082312, Length=193, Percent_Identity=27.979274611399, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44046; Mature: 44046

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTSFSPATRLGRRALLFPLCLVLFEFAAYIANDMIQPGMLAVVAEFNASVEWVPTSMTA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECCHHHH
YLAGGMFLQWLLGPLSDRRGRRPVMLAGVAFFVVTCLAILLVNSIEQFIAMRFLQGIGLC
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIGAVGYATIQESFEEAVCIKITALMANVALIAPLLGPLAGAALIHVAPWQTMFVLFAVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHH
GAISFAGLWRAMPETASLKGEKLSVANMWHDYKQVLANRRFLCGSLALGFASLPLLAWIA
HHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
QSPVILISGEQLSTFEYGILQVPIFGALIIGNLTLARLSGKTSIPQLIRYGAGPMIVGLM
CCCEEEEECCCCCHHHCCCEEHHHHHHHHHHCHHHHHHCCCCCHHHHHHHCCCHHHHHHH
IAAGSTLYSSHAYLWMTAGLSLYAFGIGLANAGLVRLTLFASDISKGTVSAAMGMISMMI
HHCCCHHHHCCCEEEECCCHHHHHHHHHHHCCCHHEEEEEHHHCCCHHHHHHHHHHHHHH
FTLGIELAKVAYLWGDSRGFNLFNLMSGLLWLGLVMVFIRRQPEAVATE
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQTSFSPATRLGRRALLFPLCLVLFEFAAYIANDMIQPGMLAVVAEFNASVEWVPTSMTA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECCHHHH
YLAGGMFLQWLLGPLSDRRGRRPVMLAGVAFFVVTCLAILLVNSIEQFIAMRFLQGIGLC
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIGAVGYATIQESFEEAVCIKITALMANVALIAPLLGPLAGAALIHVAPWQTMFVLFAVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHH
GAISFAGLWRAMPETASLKGEKLSVANMWHDYKQVLANRRFLCGSLALGFASLPLLAWIA
HHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
QSPVILISGEQLSTFEYGILQVPIFGALIIGNLTLARLSGKTSIPQLIRYGAGPMIVGLM
CCCEEEEECCCCCHHHCCCEEHHHHHHHHHHCHHHHHHCCCCCHHHHHHHCCCHHHHHHH
IAAGSTLYSSHAYLWMTAGLSLYAFGIGLANAGLVRLTLFASDISKGTVSAAMGMISMMI
HHCCCHHHHCCCEEEECCCHHHHHHHHHHHCCCHHEEEEEHHHCCCHHHHHHHHHHHHHH
FTLGIELAKVAYLWGDSRGFNLFNLMSGLLWLGLVMVFIRRQPEAVATE
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]