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The map label for this gene is malS [H]

Identifier: 170022278

GI number: 170022278

Start: 17850

End: 19913

Strand: Reverse

Name: malS [H]

Synonym: YPK_0016

Alternate gene names: 170022278

Gene position: 19913-17850 (Counterclockwise)

Preceding gene: 170022279

Following gene: 170022277

Centisome position: 0.42

GC content: 52.42

Gene sequence:

>2064_bases
ATGAAACGTCTCACACTCCCCTTGCTACTGGTTCTTTCACCAACAGCGATGGCGAACTGGTCCCTACAGCATTTTCCGGC
TTTTACTGAACAGGACAGCGGTATTTTTCTCAGTAGCAGCTCACTGACCAAAGGCGAATACCCTCTAAAATTTTATCAGG
ATAAACAGTGCTGGCAGCCAACCGGGCCGGTAAAGCTGAACCAAATGCTCTCGTTGCAGCCTTGCCAGGAGAATATCGCG
ATACCGTGGCGGTTGTTCCGTGATGGCCAGTATCAAGCGCGAATCGATACCCGTAGCGGCACCCCGACGCTGACCTTAAG
TGTCACCGCACCCGTAGCGGAGGCAGTCCAGGTCGTCACCCACGTTTGCCAACGCTGGGATGGCAATCCTGTGACCGTGG
ATGTCAGTAAAACCTTTGCCGAAGGTGAAATTGTACGGGATTTCTATTCCGGCCAAACGGCAACCGTTTCTCTCGGCAAA
ATTACGTTACGACCCGCCCCAGAGAGTGGTGGTTTACTGCTGTTGGAATCGGCCCAAACACAGCAAGCCGCGCCTTTTAG
CTGGCAAAACGCTACGGTCTATTTTGCCCTGACCGACAGATTTAATAACGGCAACCCAGCGAACGACCATAGCTATGGCC
GTCATGGCGATGGGATGCAGGAGATAGGAACGTTTCACGGTGGCGATTTGGCGGGGCTTACCGAGAAGCTGGATTATCTG
CAACAGCTTGGGGTCAACGCACTGTGGATCAGTTCTCCACTGGAACAAATTCACGGCTGGGTCGGCGGGGGGACCAAAGG
CGACTTCCCACATTATGCCTATCATGGCTACTACGGGCTGGACTGGACCCGTCTGGATGCCAATATGGGCACCGAACAGG
ATTTACGCACACTGGTTGAACAGGCACATAAACGCGGCATTCGCATCCTATTTGATGTGGTGATGAATCATGTGGGTTAT
GCAACGCTGGCGGATATGCAGAACGACCAATTCGGGGCGCTCTACCTGCAAGGCGATCAGCAGGAAAAAACCTTGGGTAA
GCGTTGGAGCGACTGGACCCCTGGCAGCGGGCAGACCTGGCACAGCTTTAATGATTACATCAACTTCAGTGATAAGACCG
CTTGGGATAATTGGTGGGGTAAAAAGTGGATCCGCACCGATATTGGTGATTACGACACCCCCGGTTATGACGATCTGACG
ATGTCGCTGGCCTTCCTACCCGATATCAAAACAGAATCGACGCAGCCCAGCGGTTTGCCAGTGTTTTACCGTAACAAACC
GGACACCGCAGCCCAAGAAATCGCCGGTGCGACACCCCGTGATTATATGACGCATTGGTTAAGCCAATGGGTACGCGATT
ACGGCATTGACGGTTTTCGGGTTGATACTGCCAAGCATGTAGAGAAACCCGCCTGGCAACAATTAAAGCAGCAGAGCATC
GCGGCACTGGCCGAATGGAAAGCCGCACATCCAGAACAGGCGCTGGATAATCTGCCATTTTGGATGACCGGAGAGGCTTG
GGGCCACGGTGTCATGAAAAGTGATTACTACCAAAATGGCTTTGATGCCATGATTAATTTTGATTTTCAGGATCAGGCAA
ATCAGGCGCTGGCCTGCTTCTCATCTATCGAGAGTACCTACAACCAAATGGCGGAGAAACTGCAAAACTTCAATGTGTTG
AGCTACCTCTCGTCTCACGATACCCGGTTATTCTTTAAAGACGATGCACAACAGTCACTGGCAAAACAGCAGCGAGCAGG
CTCTTTACTGTTGTTGGCTCCGGGGGCAGTACAAATCTTCTACGGTGATGAAAGCGGGCGGAAGTTTGGCCCAACCGGTT
CCGATCCGTTGCAGGGTACCCGTTCGGATATGAACTGGAGTGAGCTATCGGGCGAAAAAGGCGCACTGTTGGCCCATTGG
CAAAAAGTCAGTCAATTCCGCGCCCGTCATCCCGCGATAGGCGCTGGTGTACAACAATCGCAACAAACCGCCAATTACTA
TGCCTTTAGCCGCCAACATCAGGGCGATAAGGTTCTGGTCGTTTGGGTCGGTGATAAGAACTGA

Upstream 100 bases:

>100_bases
AAAAACCGCTCAGGGATGAGGCTATTTCTTCGCCAAGCGGCACACTGAATCTGCCTCAAACTTTATACGTTTCTCGAACC
CAATATTAGGAAGACGCGCC

Downstream 100 bases:

>100_bases
TATCTGGAGCTAACCGTGCGGGCGCGTGCGGTTAGCATAATGAGGCAGCCAAATTCCGCAGCTACCCCCTGCGACGTCAA
GGACGAAGGGGATACCCGAT

Product: periplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 687; Mature: 687

Protein sequence:

>687_residues
MKRLTLPLLLVLSPTAMANWSLQHFPAFTEQDSGIFLSSSSLTKGEYPLKFYQDKQCWQPTGPVKLNQMLSLQPCQENIA
IPWRLFRDGQYQARIDTRSGTPTLTLSVTAPVAEAVQVVTHVCQRWDGNPVTVDVSKTFAEGEIVRDFYSGQTATVSLGK
ITLRPAPESGGLLLLESAQTQQAAPFSWQNATVYFALTDRFNNGNPANDHSYGRHGDGMQEIGTFHGGDLAGLTEKLDYL
QQLGVNALWISSPLEQIHGWVGGGTKGDFPHYAYHGYYGLDWTRLDANMGTEQDLRTLVEQAHKRGIRILFDVVMNHVGY
ATLADMQNDQFGALYLQGDQQEKTLGKRWSDWTPGSGQTWHSFNDYINFSDKTAWDNWWGKKWIRTDIGDYDTPGYDDLT
MSLAFLPDIKTESTQPSGLPVFYRNKPDTAAQEIAGATPRDYMTHWLSQWVRDYGIDGFRVDTAKHVEKPAWQQLKQQSI
AALAEWKAAHPEQALDNLPFWMTGEAWGHGVMKSDYYQNGFDAMINFDFQDQANQALACFSSIESTYNQMAEKLQNFNVL
SYLSSHDTRLFFKDDAQQSLAKQQRAGSLLLLAPGAVQIFYGDESGRKFGPTGSDPLQGTRSDMNWSELSGEKGALLAHW
QKVSQFRARHPAIGAGVQQSQQTANYYAFSRQHQGDKVLVVWVGDKN

Sequences:

>Translated_687_residues
MKRLTLPLLLVLSPTAMANWSLQHFPAFTEQDSGIFLSSSSLTKGEYPLKFYQDKQCWQPTGPVKLNQMLSLQPCQENIA
IPWRLFRDGQYQARIDTRSGTPTLTLSVTAPVAEAVQVVTHVCQRWDGNPVTVDVSKTFAEGEIVRDFYSGQTATVSLGK
ITLRPAPESGGLLLLESAQTQQAAPFSWQNATVYFALTDRFNNGNPANDHSYGRHGDGMQEIGTFHGGDLAGLTEKLDYL
QQLGVNALWISSPLEQIHGWVGGGTKGDFPHYAYHGYYGLDWTRLDANMGTEQDLRTLVEQAHKRGIRILFDVVMNHVGY
ATLADMQNDQFGALYLQGDQQEKTLGKRWSDWTPGSGQTWHSFNDYINFSDKTAWDNWWGKKWIRTDIGDYDTPGYDDLT
MSLAFLPDIKTESTQPSGLPVFYRNKPDTAAQEIAGATPRDYMTHWLSQWVRDYGIDGFRVDTAKHVEKPAWQQLKQQSI
AALAEWKAAHPEQALDNLPFWMTGEAWGHGVMKSDYYQNGFDAMINFDFQDQANQALACFSSIESTYNQMAEKLQNFNVL
SYLSSHDTRLFFKDDAQQSLAKQQRAGSLLLLAPGAVQIFYGDESGRKFGPTGSDPLQGTRSDMNWSELSGEKGALLAHW
QKVSQFRARHPAIGAGVQQSQQTANYYAFSRQHQGDKVLVVWVGDKN
>Mature_687_residues
MKRLTLPLLLVLSPTAMANWSLQHFPAFTEQDSGIFLSSSSLTKGEYPLKFYQDKQCWQPTGPVKLNQMLSLQPCQENIA
IPWRLFRDGQYQARIDTRSGTPTLTLSVTAPVAEAVQVVTHVCQRWDGNPVTVDVSKTFAEGEIVRDFYSGQTATVSLGK
ITLRPAPESGGLLLLESAQTQQAAPFSWQNATVYFALTDRFNNGNPANDHSYGRHGDGMQEIGTFHGGDLAGLTEKLDYL
QQLGVNALWISSPLEQIHGWVGGGTKGDFPHYAYHGYYGLDWTRLDANMGTEQDLRTLVEQAHKRGIRILFDVVMNHVGY
ATLADMQNDQFGALYLQGDQQEKTLGKRWSDWTPGSGQTWHSFNDYINFSDKTAWDNWWGKKWIRTDIGDYDTPGYDDLT
MSLAFLPDIKTESTQPSGLPVFYRNKPDTAAQEIAGATPRDYMTHWLSQWVRDYGIDGFRVDTAKHVEKPAWQQLKQQSI
AALAEWKAAHPEQALDNLPFWMTGEAWGHGVMKSDYYQNGFDAMINFDFQDQANQALACFSSIESTYNQMAEKLQNFNVL
SYLSSHDTRLFFKDDAQQSLAKQQRAGSLLLLAPGAVQIFYGDESGRKFGPTGSDPLQGTRSDMNWSELSGEKGALLAHW
QKVSQFRARHPAIGAGVQQSQQTANYYAFSRQHQGDKVLVVWVGDKN

Specific function: Since only maltooligosaccharides up to a chain length of 6 glucose units are actively transported through the cytoplasmic membrane via the membrane-bound complex of three proteins, MalF, MalG, and MalK, longer maltooligosaccharides must first be degraded

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789995, Length=684, Percent_Identity=69.7368421052632, Blast_Score=986, Evalue=0.0,
Organism=Escherichia coli, GI1790687, Length=143, Percent_Identity=31.4685314685315, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322245, Length=135, Percent_Identity=31.8518518518519, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014635
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 77133; Mature: 77133

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLTLPLLLVLSPTAMANWSLQHFPAFTEQDSGIFLSSSSLTKGEYPLKFYQDKQCWQP
CCCCCCEEEEEECCHHHCCCCHHHCCCCCCCCCCEEEECCCCCCCCCCEEEECCCCCCCC
TGPVKLNQMLSLQPCQENIAIPWRLFRDGQYQARIDTRSGTPTLTLSVTAPVAEAVQVVT
CCCEEHHHHEECCCCCCCCCCCEEEEECCCEEEEEECCCCCCEEEEEECCCHHHHHHHHH
HVCQRWDGNPVTVDVSKTFAEGEIVRDFYSGQTATVSLGKITLRPAPESGGLLLLESAQT
HHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCEEEEEEEEEEEEECCCCCCEEEEECCCC
QQAAPFSWQNATVYFALTDRFNNGNPANDHSYGRHGDGMQEIGTFHGGDLAGLTEKLDYL
CCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHH
QQLGVNALWISSPLEQIHGWVGGGTKGDFPHYAYHGYYGLDWTRLDANMGTEQDLRTLVE
HHCCCCEEEECCHHHHHCCCCCCCCCCCCCCCEECCEECCEEEEECCCCCCHHHHHHHHH
QAHKRGIRILFDVVMNHVGYATLADMQNDQFGALYLQGDQQEKTLGKRWSDWTPGSGQTW
HHHHCCHHHHHHHHHHHCCCEEECCCCCCCEEEEEEECCCHHHHHHCCCCCCCCCCCCCC
HSFNDYINFSDKTAWDNWWGKKWIRTDIGDYDTPGYDDLTMSLAFLPDIKTESTQPSGLP
CCHHCEECCCCCCCCCCHHCCCHHCCCCCCCCCCCCCHHEEEEEECCCCCCCCCCCCCCE
VFYRNKPDTAAQEIAGATPRDYMTHWLSQWVRDYGIDGFRVDTAKHVEKPAWQQLKQQSI
EEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECHHHHHCCHHHHHHHHHHH
AALAEWKAAHPEQALDNLPFWMTGEAWGHGVMKSDYYQNGFDAMINFDFQDQANQALACF
HHHHHHHCCCHHHHHHCCCEEEECHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHH
SSIESTYNQMAEKLQNFNVLSYLSSHDTRLFFKDDAQQSLAKQQRAGSLLLLAPGAVQIF
HHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEECCCHHHHHHHHHHCCCEEEECCCEEEEE
YGDESGRKFGPTGSDPLQGTRSDMNWSELSGEKGALLAHWQKVSQFRARHPAIGAGVQQS
ECCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHH
QQTANYYAFSRQHQGDKVLVVWVGDKN
HHHHHHHEECCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MKRLTLPLLLVLSPTAMANWSLQHFPAFTEQDSGIFLSSSSLTKGEYPLKFYQDKQCWQP
CCCCCCEEEEEECCHHHCCCCHHHCCCCCCCCCCEEEECCCCCCCCCCEEEECCCCCCCC
TGPVKLNQMLSLQPCQENIAIPWRLFRDGQYQARIDTRSGTPTLTLSVTAPVAEAVQVVT
CCCEEHHHHEECCCCCCCCCCCEEEEECCCEEEEEECCCCCCEEEEEECCCHHHHHHHHH
HVCQRWDGNPVTVDVSKTFAEGEIVRDFYSGQTATVSLGKITLRPAPESGGLLLLESAQT
HHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCEEEEEEEEEEEEECCCCCCEEEEECCCC
QQAAPFSWQNATVYFALTDRFNNGNPANDHSYGRHGDGMQEIGTFHGGDLAGLTEKLDYL
CCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHH
QQLGVNALWISSPLEQIHGWVGGGTKGDFPHYAYHGYYGLDWTRLDANMGTEQDLRTLVE
HHCCCCEEEECCHHHHHCCCCCCCCCCCCCCCEECCEECCEEEEECCCCCCHHHHHHHHH
QAHKRGIRILFDVVMNHVGYATLADMQNDQFGALYLQGDQQEKTLGKRWSDWTPGSGQTW
HHHHCCHHHHHHHHHHHCCCEEECCCCCCCEEEEEEECCCHHHHHHCCCCCCCCCCCCCC
HSFNDYINFSDKTAWDNWWGKKWIRTDIGDYDTPGYDDLTMSLAFLPDIKTESTQPSGLP
CCHHCEECCCCCCCCCCHHCCCHHCCCCCCCCCCCCCHHEEEEEECCCCCCCCCCCCCCE
VFYRNKPDTAAQEIAGATPRDYMTHWLSQWVRDYGIDGFRVDTAKHVEKPAWQQLKQQSI
EEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECHHHHHCCHHHHHHHHHHH
AALAEWKAAHPEQALDNLPFWMTGEAWGHGVMKSDYYQNGFDAMINFDFQDQANQALACF
HHHHHHHCCCHHHHHHCCCEEEECHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHH
SSIESTYNQMAEKLQNFNVLSYLSSHDTRLFFKDDAQQSLAKQQRAGSLLLLAPGAVQIF
HHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEECCCHHHHHHHHHHCCCEEEECCCEEEEE
YGDESGRKFGPTGSDPLQGTRSDMNWSELSGEKGALLAHWQKVSQFRARHPAIGAGVQQS
ECCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHH
QQTANYYAFSRQHQGDKVLVVWVGDKN
HHHHHHHEECCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1544897; 8041620; 9278503; 9268356 [H]