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The map label for this gene is tuf

Identifier: 169630927

GI number: 169630927

Start: 3895074

End: 3896267

Strand: Reverse

Name: tuf

Synonym: MAB_3848c

Alternate gene names: 169630927

Gene position: 3896267-3895074 (Counterclockwise)

Preceding gene: 169630928

Following gene: 169630924

Centisome position: 76.89

GC content: 63.15

Gene sequence:

>1194_bases
GTGGCGAAGGCGAAGTTCGAGCGGACGAAGCCGCACGTCAACATCGGGACCATCGGTCACGTTGACCACGGCAAGACCAC
TCTCACTGCAGCTATCACCAAGGTGCTGCATGACAAGTACCCCGATTTGAACGAGGCGTCGGCCTTCGACCAGATCGACA
ACGCTCCCGAAGAGAAGGCTCGTGGTATCACGATCAACATCTCGCACGTGGAGTACCAGACCGAGAAGCGTCACTACGCA
CACGTCGACGCCCCCGGTCACGCTGACTACATCAAGAACATGATCACCGGTGCCGCCCAGATGGACGGCGCGATCCTGGT
GGTTGCCGCGACCGACGGTCCCATGCCGCAGACCCGCGAGCACGTGCTGCTCGCCCGTCAGGTGGGTGTGCCCTACATCC
TCGTCGCGCTGAACAAGTCCGACATGGTGGACGACGAGGAGCTCCTGGAGCTCGTCGAGATGGAGGTCCGGGAACTGCTG
AGCAGCCAGGACTTCGACGGCGACAACGCTCCCGTCGTGCGTGTCTCGGCGCTGAAGGCGCTCGAGGGCGACGCCGAGTG
GGGCAAGACCGTTGCCGACCTGATGGATGCGGTTGACGAGTCCATCCCGGATCCGGTTCGCGAGACCGAGAAGCCGTTCC
TGATGCCCGTCGAGGACGTGTTCACCATCACCGGTCGTGGCACCGTGGTGACCGGTCGCGTCGAGCGCGGCGTGATCAAC
GTGAACGAGGATGTCGAGATCGTCGGCATCAAGGACACCACCACCAAGACCACGGTCACCGGTGTTGAGATGTTCCGCAA
GCTGCTGGACCAGGGCCAGGCCGGCGACAACGTCGGTCTGCTGGTTCGTGGTGTGAAGCGTGAGGACGTCGAGCGTGGCC
AGGTTGTGGTCAAGCCCGGCACCACCACCCCGCACACCGAGTTCGAGGGCAGCGTCTACATCCTGTCCAAGGACGAGGGT
GGCCGCCACACGCCGTTCTTCAACAACTACCGTCCGCAGTTCTACTTCCGCACCACCGACGTGACCGGTGTTGTGACGCT
GCCGGAAGGTACCGAGATGGTTATGCCCGGTGACAACACCGACATCTCCGTCAAGCTGATCCAGCCGGTCGCCATGGACG
AGGGTCTGCGTTTCGCCATCCGTGAGGGCGGTCGCACCGTCGGCGCGGGTCGCGTCACCAAGATCATCAAGTGA

Upstream 100 bases:

>100_bases
CGAAGCGAACGACCCAATTGGGCTTGCGCGAGTAGGTCCTAAGATCGCGCCCCTGGCGCAAAACTGTAAGTAACAACAAC
AAGAGTCCAGGAGGACACAA

Downstream 100 bases:

>100_bases
TCATCTGGTCGCTCTAAGGAACGCCAAGAAAACGGCGCTCACCCTTCGGGGTGGGCGCCGTTTTTCGTGGTGTGCCAACC
TCGCAAAGTCGGCGTCGGGC

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKYPDLNEASAFDQIDNAPEEKARGITINISHVEYQTEKRHYA
HVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKSDMVDDEELLELVEMEVRELL
SSQDFDGDNAPVVRVSALKALEGDAEWGKTVADLMDAVDESIPDPVRETEKPFLMPVEDVFTITGRGTVVTGRVERGVIN
VNEDVEIVGIKDTTTKTTVTGVEMFRKLLDQGQAGDNVGLLVRGVKREDVERGQVVVKPGTTTPHTEFEGSVYILSKDEG
GRHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTDISVKLIQPVAMDEGLRFAIREGGRTVGAGRVTKIIK

Sequences:

>Translated_397_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKYPDLNEASAFDQIDNAPEEKARGITINISHVEYQTEKRHYA
HVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKSDMVDDEELLELVEMEVRELL
SSQDFDGDNAPVVRVSALKALEGDAEWGKTVADLMDAVDESIPDPVRETEKPFLMPVEDVFTITGRGTVVTGRVERGVIN
VNEDVEIVGIKDTTTKTTVTGVEMFRKLLDQGQAGDNVGLLVRGVKREDVERGQVVVKPGTTTPHTEFEGSVYILSKDEG
GRHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTDISVKLIQPVAMDEGLRFAIREGGRTVGAGRVTKIIK
>Mature_396_residues
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKYPDLNEASAFDQIDNAPEEKARGITINISHVEYQTEKRHYAH
VDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKSDMVDDEELLELVEMEVRELLS
SQDFDGDNAPVVRVSALKALEGDAEWGKTVADLMDAVDESIPDPVRETEKPFLMPVEDVFTITGRGTVVTGRVERGVINV
NEDVEIVGIKDTTTKTTVTGVEMFRKLLDQGQAGDNVGLLVRGVKREDVERGQVVVKPGTTTPHTEFEGSVYILSKDEGG
RHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTDISVKLIQPVAMDEGLRFAIREGGRTVGAGRVTKIIK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=397, Percent_Identity=55.6675062972292, Blast_Score=454, Evalue=1e-128,
Organism=Homo sapiens, GI4503471, Length=457, Percent_Identity=31.0722100656455, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI4503475, Length=352, Percent_Identity=34.0909090909091, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI223555963, Length=441, Percent_Identity=29.4784580498866, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI5729864, Length=441, Percent_Identity=29.4784580498866, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI54607086, Length=326, Percent_Identity=28.2208588957055, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI194018522, Length=330, Percent_Identity=27.8787878787879, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI194097354, Length=334, Percent_Identity=27.5449101796407, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI194018520, Length=334, Percent_Identity=27.5449101796407, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI46094014, Length=328, Percent_Identity=28.0487804878049, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI4503507, Length=356, Percent_Identity=26.4044943820225, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI82546879, Length=436, Percent_Identity=22.2477064220184, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI157426893, Length=286, Percent_Identity=25.1748251748252, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=76.2626262626263, Blast_Score=592, Evalue=1e-170,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=76.2626262626263, Blast_Score=591, Evalue=1e-170,
Organism=Escherichia coli, GI2367247, Length=340, Percent_Identity=30.8823529411765, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1789108, Length=336, Percent_Identity=26.7857142857143, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI48994988, Length=288, Percent_Identity=29.5138888888889, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1788922, Length=331, Percent_Identity=25.0755287009063, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=406, Percent_Identity=49.7536945812808, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI25141371, Length=374, Percent_Identity=45.1871657754011, Blast_Score=318, Evalue=4e-87,
Organism=Caenorhabditis elegans, GI17552884, Length=334, Percent_Identity=35.9281437125749, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=334, Percent_Identity=35.9281437125749, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=320, Percent_Identity=33.75, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI115532067, Length=439, Percent_Identity=27.1070615034169, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=439, Percent_Identity=27.1070615034169, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=274, Percent_Identity=31.3868613138686, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=454, Percent_Identity=24.0088105726872, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32566301, Length=150, Percent_Identity=41.3333333333333, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=273, Percent_Identity=26.3736263736264, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=233, Percent_Identity=24.4635193133047, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=402, Percent_Identity=63.4328358208955, Blast_Score=503, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6325337, Length=353, Percent_Identity=33.9943342776204, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=353, Percent_Identity=33.9943342776204, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=338, Percent_Identity=26.9230769230769, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6320377, Length=330, Percent_Identity=28.4848484848485, Blast_Score=93, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=266, Percent_Identity=27.0676691729323, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=238, Percent_Identity=26.890756302521, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281363316, Length=396, Percent_Identity=59.5959595959596, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI17864358, Length=396, Percent_Identity=59.5959595959596, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI19921738, Length=389, Percent_Identity=51.413881748072, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI45553807, Length=450, Percent_Identity=30.4444444444444, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI45553816, Length=450, Percent_Identity=30.4444444444444, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24651721, Length=450, Percent_Identity=30.4444444444444, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI17864154, Length=450, Percent_Identity=30.4444444444444, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=30.2895322939866, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=30.2895322939866, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI19922690, Length=306, Percent_Identity=31.0457516339869, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI45550900, Length=337, Percent_Identity=29.9703264094956, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17137380, Length=319, Percent_Identity=28.5266457680251, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24647048, Length=357, Percent_Identity=25.2100840336134, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24647046, Length=357, Percent_Identity=25.2100840336134, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28572034, Length=184, Percent_Identity=36.4130434782609, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI78706572, Length=261, Percent_Identity=27.2030651340996, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_MYCA9 (B1MGH7)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001704576.1
- ProteinModelPortal:   B1MGH7
- SMR:   B1MGH7
- EnsemblBacteria:   EBMYCT00000008900
- GeneID:   5966318
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_3848c
- GeneTree:   EBGT00050000014852
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- ProtClustDB:   PRK00049
- BioCyc:   MABS561007:MAB_3848C-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 43528; Mature: 43397

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKYPDLNEASAFDQIDNAPEEKA
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHH
RGITINISHVEYQTEKRHYAHVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTRE
CCEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHH
HVLLARQVGVPYILVALNKSDMVDDEELLELVEMEVRELLSSQDFDGDNAPVVRVSALKA
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHH
LEGDAEWGKTVADLMDAVDESIPDPVRETEKPFLMPVEDVFTITGRGTVVTGRVERGVIN
HCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEHHHEEEEECCCEEEEEEECCCEEE
VNEDVEIVGIKDTTTKTTVTGVEMFRKLLDQGQAGDNVGLLVRGVKREDVERGQVVVKPG
CCCCEEEEEECCCCCCEEHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEECC
TTTPHTEFEGSVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNT
CCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCC
DISVKLIQPVAMDEGLRFAIREGGRTVGAGRVTKIIK
CEEEEEECCCHHCCCCEEEECCCCCEECCCHHHHHCC
>Mature Secondary Structure 
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKYPDLNEASAFDQIDNAPEEKA
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHH
RGITINISHVEYQTEKRHYAHVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTRE
CCEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHH
HVLLARQVGVPYILVALNKSDMVDDEELLELVEMEVRELLSSQDFDGDNAPVVRVSALKA
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHH
LEGDAEWGKTVADLMDAVDESIPDPVRETEKPFLMPVEDVFTITGRGTVVTGRVERGVIN
HCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEHHHEEEEECCCEEEEEEECCCEEE
VNEDVEIVGIKDTTTKTTVTGVEMFRKLLDQGQAGDNVGLLVRGVKREDVERGQVVVKPG
CCCCEEEEEECCCCCCEEHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEECC
TTTPHTEFEGSVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNT
CCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCC
DISVKLIQPVAMDEGLRFAIREGGRTVGAGRVTKIIK
CEEEEEECCCHHCCCCEEEECCCCCEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA