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The map label for this gene is thcD [H]

Identifier: 169630917

GI number: 169630917

Start: 3883961

End: 3885157

Strand: Reverse

Name: thcD [H]

Synonym: MAB_3838c

Alternate gene names: 169630917

Gene position: 3885157-3883961 (Counterclockwise)

Preceding gene: 169630918

Following gene: 169630915

Centisome position: 76.67

GC content: 66.67

Gene sequence:

>1197_bases
ATGACCGACGCGGAACGTGGAGTGCTGATCATCGGTGGTGGGCTGGGCGCGGTACGAACCGCCGAACAGTTGCGCCGTGC
GGAGTTCACCGGGCCAATCACCATCGTCAGTAGTGAGACCCATCTGCCGTACGACCGGCCGCCGCTCTCCAAGGATGTCC
TGCGTGACGCGGAGAAGACGATCGACGCTGTGGTGCTCAAACCGCGAGAGTTTTACGACGAGAAGCAGATTGAGTTGCGT
CTCGGCTCCGAGGTCACCGCGCTGGACCCGGTGGCCCGCACGGTCACCCTGAGTGACGGCGGCACCTTGGAGTACGGAGA
GGTGATCATCGCGACCGGTTTGGTGCCCCGTCGCATTCCTACTTTCCCAGACTTGGCTGGTGTTCATGTACTGCGCACGG
CCGACGACAGCTTCGCGCTGCGTGGCGACGCCGAGAACGCGCGGCGGGCGGTCGTCGTCGGGGCAGGTTTCATCGGCTGC
GAGGTGGCGGCCACCCTGCGGGCCAACGGAGTTGAGGTGGTCCTGGTGGAGCCGCAGCCGGCGCCGCTGTTGGCGGCTAT
CGGCCAGCAGCTCGGGGATCTGGTGGCCCGGCTGCACCGCAACGAGGGTGTGGACGTTCGGGTGGGCGTCGGCGTGGACG
CCGTGGAAGGACAGGACCGGGTCCAGTCCGTCACCTTGTCGGATGGGACGCGCCTGGACACCGACCTAGTCGTCCTGGGT
ATCGGGAGCCGCCCCGCGATCGATTGGCTGGACGGGTCTGGCGTGGCGGTGGACAACGGCGTGGTGTGTGACGCGGTGGG
CGCCACCGGTACTCCGCATGTGTGGGCGCTGGGTGATGTCGCCGCCTGGGCGGGCGCCGACGGGCGGCCGAACCGGGTGG
AGCACTGGAGCAATGTCGCCGATCAGGTGCGTGCGCTGGTTCCGGCCCTGCTGGGGCAAGAAACGTCTGCCAATACGGTT
GCAGTGCCCTATTTTTGGAGTGACCAGTACGACGTCAAGATTCAGTCGCTCGGGCACCTCGGAAGCTCCGACACCGTGCA
CCTGATCTCCGATGACGGCCGGAAGTTCCTGGCCTATTTGGAACGCGACGGCGTGCTGACCGGGGTCGTGGGCTGCGGCA
TGGCAGGCCCGGTGATGAAGATGCGGGCCAAGATCGCGGCCGGAACACCGATCGCCGAGGTACTCGCGGGGTCTTAG

Upstream 100 bases:

>100_bases
ACCGAGAGCATCGTGGTGCTTTCCAACCTGGTGGACCGGGCGCAGGTGCAGGGGTGATAGGGCTGACATCGCGCGTGTCC
AGTCGTGTATGAAGAAACCC

Downstream 100 bases:

>100_bases
GGGATTAGCGCAGGGCGCGCAGCCCCACCTCGGCGGCATCGAAGCCCTTGCCGAGCAGCGTGGTGAGCTTCGAGGACTCG
TCGGCGAGCCATTGTTCATA

Product: putative ferredoxin reductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTDAERGVLIIGGGLGAVRTAEQLRRAEFTGPITIVSSETHLPYDRPPLSKDVLRDAEKTIDAVVLKPREFYDEKQIELR
LGSEVTALDPVARTVTLSDGGTLEYGEVIIATGLVPRRIPTFPDLAGVHVLRTADDSFALRGDAENARRAVVVGAGFIGC
EVAATLRANGVEVVLVEPQPAPLLAAIGQQLGDLVARLHRNEGVDVRVGVGVDAVEGQDRVQSVTLSDGTRLDTDLVVLG
IGSRPAIDWLDGSGVAVDNGVVCDAVGATGTPHVWALGDVAAWAGADGRPNRVEHWSNVADQVRALVPALLGQETSANTV
AVPYFWSDQYDVKIQSLGHLGSSDTVHLISDDGRKFLAYLERDGVLTGVVGCGMAGPVMKMRAKIAAGTPIAEVLAGS

Sequences:

>Translated_398_residues
MTDAERGVLIIGGGLGAVRTAEQLRRAEFTGPITIVSSETHLPYDRPPLSKDVLRDAEKTIDAVVLKPREFYDEKQIELR
LGSEVTALDPVARTVTLSDGGTLEYGEVIIATGLVPRRIPTFPDLAGVHVLRTADDSFALRGDAENARRAVVVGAGFIGC
EVAATLRANGVEVVLVEPQPAPLLAAIGQQLGDLVARLHRNEGVDVRVGVGVDAVEGQDRVQSVTLSDGTRLDTDLVVLG
IGSRPAIDWLDGSGVAVDNGVVCDAVGATGTPHVWALGDVAAWAGADGRPNRVEHWSNVADQVRALVPALLGQETSANTV
AVPYFWSDQYDVKIQSLGHLGSSDTVHLISDDGRKFLAYLERDGVLTGVVGCGMAGPVMKMRAKIAAGTPIAEVLAGS
>Mature_397_residues
TDAERGVLIIGGGLGAVRTAEQLRRAEFTGPITIVSSETHLPYDRPPLSKDVLRDAEKTIDAVVLKPREFYDEKQIELRL
GSEVTALDPVARTVTLSDGGTLEYGEVIIATGLVPRRIPTFPDLAGVHVLRTADDSFALRGDAENARRAVVVGAGFIGCE
VAATLRANGVEVVLVEPQPAPLLAAIGQQLGDLVARLHRNEGVDVRVGVGVDAVEGQDRVQSVTLSDGTRLDTDLVVLGI
GSRPAIDWLDGSGVAVDNGVVCDAVGATGTPHVWALGDVAAWAGADGRPNRVEHWSNVADQVRALVPALLGQETSANTVA
VPYFWSDQYDVKIQSLGHLGSSDTVHLISDDGRKFLAYLERDGVLTGVVGCGMAGPVMKMRAKIAAGTPIAEVLAGS

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=392, Percent_Identity=29.3367346938776, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI226437568, Length=392, Percent_Identity=29.3367346938776, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI21389617, Length=392, Percent_Identity=29.3367346938776, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI22202629, Length=351, Percent_Identity=27.6353276353276, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI4757732, Length=351, Percent_Identity=27.6353276353276, Blast_Score=99, Evalue=7e-21,
Organism=Escherichia coli, GI1788892, Length=385, Percent_Identity=28.5714285714286, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1789065, Length=279, Percent_Identity=25.089605734767, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789765, Length=284, Percent_Identity=28.169014084507, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87082354, Length=275, Percent_Identity=26.1818181818182, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17559934, Length=331, Percent_Identity=31.7220543806647, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI32564386, Length=426, Percent_Identity=24.1784037558685, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585130, Length=400, Percent_Identity=28, Blast_Score=147, Evalue=9e-36,
Organism=Drosophila melanogaster, GI24639257, Length=396, Percent_Identity=29.2929292929293, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI281359715, Length=393, Percent_Identity=29.5165394402036, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI281359713, Length=393, Percent_Identity=29.5165394402036, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24639250, Length=393, Percent_Identity=29.5165394402036, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI18543267, Length=393, Percent_Identity=29.5165394402036, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24639252, Length=393, Percent_Identity=29.5165394402036, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24581020, Length=356, Percent_Identity=26.9662921348315, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI28573993, Length=356, Percent_Identity=26.9662921348315, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 41851; Mature: 41720

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAERGVLIIGGGLGAVRTAEQLRRAEFTGPITIVSSETHLPYDRPPLSKDVLRDAEKT
CCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
IDAVVLKPREFYDEKQIELRLGSEVTALDPVARTVTLSDGGTLEYGEVIIATGLVPRRIP
HHEEEECCHHHCCCCEEEEEECCCCEEECCCEEEEEECCCCCEEECCEEEEECCCCCCCC
TFPDLAGVHVLRTADDSFALRGDAENARRAVVVGAGFIGCEVAATLRANGVEVVLVEPQP
CCCCCCCEEEEEECCCCEEEECCCCCCCEEEEEECCEECCEEEEEEECCCEEEEEECCCC
APLLAAIGQQLGDLVARLHRNEGVDVRVGVGVDAVEGQDRVQSVTLSDGTRLDTDLVVLG
CHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHEEEEECCCCEECCCEEEEE
IGSRPAIDWLDGSGVAVDNGVVCDAVGATGTPHVWALGDVAAWAGADGRPNRVEHWSNVA
CCCCCCEEEECCCCEEECCCEEEEECCCCCCCCEEEECCHHHHCCCCCCCCHHHHHHHHH
DQVRALVPALLGQETSANTVAVPYFWSDQYDVKIQSLGHLGSSDTVHLISDDGRKFLAYL
HHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEEECCCCCCCCEEEEECCCCHHHHHHH
ERDGVLTGVVGCGMAGPVMKMRAKIAAGTPIAEVLAGS
HCCCCEEEHHHCCCCCHHHHHHHHHCCCCCHHHHHCCC
>Mature Secondary Structure 
TDAERGVLIIGGGLGAVRTAEQLRRAEFTGPITIVSSETHLPYDRPPLSKDVLRDAEKT
CCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
IDAVVLKPREFYDEKQIELRLGSEVTALDPVARTVTLSDGGTLEYGEVIIATGLVPRRIP
HHEEEECCHHHCCCCEEEEEECCCCEEECCCEEEEEECCCCCEEECCEEEEECCCCCCCC
TFPDLAGVHVLRTADDSFALRGDAENARRAVVVGAGFIGCEVAATLRANGVEVVLVEPQP
CCCCCCCEEEEEECCCCEEEECCCCCCCEEEEEECCEECCEEEEEEECCCEEEEEECCCC
APLLAAIGQQLGDLVARLHRNEGVDVRVGVGVDAVEGQDRVQSVTLSDGTRLDTDLVVLG
CHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHEEEEECCCCEECCCEEEEE
IGSRPAIDWLDGSGVAVDNGVVCDAVGATGTPHVWALGDVAAWAGADGRPNRVEHWSNVA
CCCCCCEEEECCCCEEECCCEEEEECCCCCCCCEEEECCHHHHCCCCCCCCHHHHHHHHH
DQVRALVPALLGQETSANTVAVPYFWSDQYDVKIQSLGHLGSSDTVHLISDDGRKFLAYL
HHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEEECCCCCCCCEEEEECCCCHHHHHHH
ERDGVLTGVVGCGMAGPVMKMRAKIAAGTPIAEVLAGS
HCCCCEEEHHHCCCCCHHHHHHHHHCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]