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The map label for this gene is hsaD [H]

Identifier: 169630890

GI number: 169630890

Start: 3862159

End: 3863019

Strand: Direct

Name: hsaD [H]

Synonym: MAB_3810

Alternate gene names: 169630890

Gene position: 3862159-3863019 (Clockwise)

Preceding gene: 169630872

Following gene: 169630902

Centisome position: 76.22

GC content: 63.76

Gene sequence:

>861_bases
GTGGACGTGACATACGAGGGCACCAAGAGAGAACTGGCCACCGACGAGGGGACGCTGCGCTACCACGAAGCGGGCACAGC
CGGCGAGGGCGAGCCGCTGATCCTGCTGCACGGGTCCGGCCCCGGCGTGACCGGTTGGCGCAACTATCGCGGAAACCTCG
GGGTCTTCGCCAAGCATTTCCACACCTACGTTCTGGAATTTCCCGGCTTCGGCGTGAGCGACGCAGTGCCGGGACACCCG
GTCCTGACGGCTGGTGCCTCGGTGATCCGCTTCATGGACGGCCTCGGCATCTCCTCGGCCGCCGTCGTCGGCAACTCGAT
GGGCGGTGTCGTCGGCATCAACCTGGCGATCAAGCACCCCGACCGCATCACCAAACTCGTCACCATCGGTGGTGTCGGAC
CGAATATCTTCAGCCCCAGCCCGAGCGAGGGCCTGCGCCTGCTGCAGGAGTTCACCGATAACCCGGATCGCGACAAGCTG
ATCCGCTGGCTCAACTCCATGGTGTGGGATCCCGCCGTCGTCACCGACGAGCTCATCGAGGAGCGTTGGGAGGCCGCGAT
CAACCCGGACGCGCACAAGACCGCGCAATTGATGTACGGCTCCAAAGCCTTTGCGATGCAACAGCAGTTCCTCGCGAATG
CCGACATCCCGCCGTACTGGGCGATGATGCACAAGATCAAGTGCCCGACACTGCTGACCTGGGGGCGCGACGACAGGGTC
AGCCCACCGGATATGGCGATCGTGCCCATGCGGCTCATTCCCAACGCCGAATTGCACATCTTCCCCAACAGTGGGCATTG
GGTGATGATCGAGGCCAAGCAGGCCTTCGAGCAGACGGTCGTCGACTTTTTGCGGCGCTAA

Upstream 100 bases:

>100_bases
TACTCGTGCCTAATCCGAGTGTCCGAACCGGCGGGCGCGCCCTACCCACTCAGTGGACACCGGCGTCGCCCGAAACTGAA
ACACGTTCTACTCTTGTCTG

Downstream 100 bases:

>100_bases
TCCTCGAATCCTGCGCTGCCAACGCGAAAGCGCGCCGCCCCTTTCGGGAGCGGCGCGCTTTGTGCGTATGACGCGGTCTT
ACTTGGCCTTCTCGAGGACC

Product: putative hydrolase, alpha/beta fold

Products: NA

Alternate protein names: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase; HOPDA hydrolase; Meta-cleavage product hydrolase; MCP hydrolase [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MDVTYEGTKRELATDEGTLRYHEAGTAGEGEPLILLHGSGPGVTGWRNYRGNLGVFAKHFHTYVLEFPGFGVSDAVPGHP
VLTAGASVIRFMDGLGISSAAVVGNSMGGVVGINLAIKHPDRITKLVTIGGVGPNIFSPSPSEGLRLLQEFTDNPDRDKL
IRWLNSMVWDPAVVTDELIEERWEAAINPDAHKTAQLMYGSKAFAMQQQFLANADIPPYWAMMHKIKCPTLLTWGRDDRV
SPPDMAIVPMRLIPNAELHIFPNSGHWVMIEAKQAFEQTVVDFLRR

Sequences:

>Translated_286_residues
MDVTYEGTKRELATDEGTLRYHEAGTAGEGEPLILLHGSGPGVTGWRNYRGNLGVFAKHFHTYVLEFPGFGVSDAVPGHP
VLTAGASVIRFMDGLGISSAAVVGNSMGGVVGINLAIKHPDRITKLVTIGGVGPNIFSPSPSEGLRLLQEFTDNPDRDKL
IRWLNSMVWDPAVVTDELIEERWEAAINPDAHKTAQLMYGSKAFAMQQQFLANADIPPYWAMMHKIKCPTLLTWGRDDRV
SPPDMAIVPMRLIPNAELHIFPNSGHWVMIEAKQAFEQTVVDFLRR
>Mature_286_residues
MDVTYEGTKRELATDEGTLRYHEAGTAGEGEPLILLHGSGPGVTGWRNYRGNLGVFAKHFHTYVLEFPGFGVSDAVPGHP
VLTAGASVIRFMDGLGISSAAVVGNSMGGVVGINLAIKHPDRITKLVTIGGVGPNIFSPSPSEGLRLLQEFTDNPDRDKL
IRWLNSMVWDPAVVTDELIEERWEAAINPDAHKTAQLMYGSKAFAMQQQFLANADIPPYWAMMHKIKCPTLLTWGRDDRV
SPPDMAIVPMRLIPNAELHIFPNSGHWVMIEAKQAFEQTVVDFLRR

Specific function: Catalyzes the hydrolysis of a carbon-carbon bond in 4,5- 9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid (4,9-DSHA) to yield 9,17-dioxo-1,2,3,4,10,19- hexanorandrostan-5-oic acid (DOHNAA) and 2-hydroxy-hexa-2,4- dienoic acid (HHD). Is

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. HsaD family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=290, Percent_Identity=33.448275862069, Blast_Score=149, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.7.1.8 [H]

Molecular weight: Translated: 31484; Mature: 31484

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVTYEGTKRELATDEGTLRYHEAGTAGEGEPLILLHGSGPGVTGWRNYRGNLGVFAKHF
CCCCCCCCCHHHCCCCCCEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHH
HTYVLEFPGFGVSDAVPGHPVLTAGASVIRFMDGLGISSAAVVGNSMGGVVGINLAIKHP
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEEEEEECC
DRITKLVTIGGVGPNIFSPSPSEGLRLLQEFTDNPDRDKLIRWLNSMVWDPAVVTDELIE
HHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHH
ERWEAAINPDAHKTAQLMYGSKAFAMQQQFLANADIPPYWAMMHKIKCPTLLTWGRDDRV
HHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEECCCCCCC
SPPDMAIVPMRLIPNAELHIFPNSGHWVMIEAKQAFEQTVVDFLRR
CCCCEEEEEHEECCCCEEEEEECCCCEEEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDVTYEGTKRELATDEGTLRYHEAGTAGEGEPLILLHGSGPGVTGWRNYRGNLGVFAKHF
CCCCCCCCCHHHCCCCCCEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHH
HTYVLEFPGFGVSDAVPGHPVLTAGASVIRFMDGLGISSAAVVGNSMGGVVGINLAIKHP
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEEEEEECC
DRITKLVTIGGVGPNIFSPSPSEGLRLLQEFTDNPDRDKLIRWLNSMVWDPAVVTDELIE
HHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHH
ERWEAAINPDAHKTAQLMYGSKAFAMQQQFLANADIPPYWAMMHKIKCPTLLTWGRDDRV
HHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEECCCCCCC
SPPDMAIVPMRLIPNAELHIFPNSGHWVMIEAKQAFEQTVVDFLRR
CCCCEEEEEHEECCCCEEEEEECCCCEEEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]