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The map label for this gene is folD

Identifier: 169630771

GI number: 169630771

Start: 3746551

End: 3747396

Strand: Reverse

Name: folD

Synonym: MAB_3692c

Alternate gene names: 169630771

Gene position: 3747396-3746551 (Counterclockwise)

Preceding gene: 169630772

Following gene: 169630770

Centisome position: 73.95

GC content: 68.2

Gene sequence:

>846_bases
GTGACGGCGACTCGACTTGATGGCAAGGCCACCCGCGATGAGATCTTTGTGGAGCTGGCCACTCGTGCCGCGGCGCTGAA
GGCGGCGGGCAAGACACCGGGGCTGGGCACCATCCTGGTGGGTGACGACCCGGGTTCGCACGCCTATGTGCGCGGCAAGC
ACGCAGACTGCGCCAAGGTGGGCCTCAACTCGATCCGTCGTGATCTGCCCGCCGATATCGATCAGGCCACACTGTGCGCC
ACCATCGACGAGCTGAACGCCAACCCGGATTGCACCGGCTACATCGTGCAGCTGCCGCTGCCCAAGCATCTTGACGAGAA
CGAGGCGCTGGAGCGCATCGACCCGGACAAGGACGCCGACGGCCTGCACCCGACGAACCTGGGCCGGCTGGTGCTCGGCA
AGGAGGCCCCGCTTCCCTGCACGCCCCGCGGGATCGTGTATCTGCTGCGTCGCTATGACGTGAAGCTCGACGGGGCACAC
GTCGTGGTGATCGGTCGCGGTGTCACGGTGGGGCGTCCACTCGGGCTGCTGCTGACCCGGCGCTCGGAGAACGCGACAGT
CACCCTGTGCCACACCGGAACCCGTGATCTGGCCGCGCTGACCCGGCAGGCCGACATCATCGTGGCGGCCGCCGGTGTGC
CGCACATGGTGACGGCGGACATGGTCAAGCCGGGTGCCGCGGTGGTCGACGTCGGCGTGAGCCGGGTCGACGACAAGCTA
ACCGGCGACGTGGCCGAGGATGTCTGGGACGTGGCCGGGTACGTCTCGCCCAACCCCGGCGGAGTCGGGCCGCTGACCCG
GGCCTTCTTGCTCACCAATGTGATCGAGCGGGCGGAACGTTCGTGA

Upstream 100 bases:

>100_bases
GCCAAGCGTCAGCCACTCGCCGACGTCCTGCACTGGGACACGTGGTGAGGGGCGATGAGCCGCTTGCGCGAAGAGCTGTA
GGCCCAGGAATACTAGCCGT

Downstream 100 bases:

>100_bases
CCGCCCTGCAACCCGGGCCGCGGCGTCTTCTCCCGACAGCGTTGGACGCGATCCAGCGGTTCGCCCGTGAGCAGTGGCCG
ATCCTGGTGGTCTCGGCGGT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/methenyl tetrahydrofolate cyclohydrolase FolD

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MTATRLDGKATRDEIFVELATRAAALKAAGKTPGLGTILVGDDPGSHAYVRGKHADCAKVGLNSIRRDLPADIDQATLCA
TIDELNANPDCTGYIVQLPLPKHLDENEALERIDPDKDADGLHPTNLGRLVLGKEAPLPCTPRGIVYLLRRYDVKLDGAH
VVVIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRDLAALTRQADIIVAAAGVPHMVTADMVKPGAAVVDVGVSRVDDKL
TGDVAEDVWDVAGYVSPNPGGVGPLTRAFLLTNVIERAERS

Sequences:

>Translated_281_residues
MTATRLDGKATRDEIFVELATRAAALKAAGKTPGLGTILVGDDPGSHAYVRGKHADCAKVGLNSIRRDLPADIDQATLCA
TIDELNANPDCTGYIVQLPLPKHLDENEALERIDPDKDADGLHPTNLGRLVLGKEAPLPCTPRGIVYLLRRYDVKLDGAH
VVVIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRDLAALTRQADIIVAAAGVPHMVTADMVKPGAAVVDVGVSRVDDKL
TGDVAEDVWDVAGYVSPNPGGVGPLTRAFLLTNVIERAERS
>Mature_280_residues
TATRLDGKATRDEIFVELATRAAALKAAGKTPGLGTILVGDDPGSHAYVRGKHADCAKVGLNSIRRDLPADIDQATLCAT
IDELNANPDCTGYIVQLPLPKHLDENEALERIDPDKDADGLHPTNLGRLVLGKEAPLPCTPRGIVYLLRRYDVKLDGAHV
VVIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRDLAALTRQADIIVAAAGVPHMVTADMVKPGAAVVDVGVSRVDDKLT
GDVAEDVWDVAGYVSPNPGGVGPLTRAFLLTNVIERAERS

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=41.8367346938776, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI222418558, Length=265, Percent_Identity=43.0188679245283, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI222136639, Length=292, Percent_Identity=35.958904109589, Blast_Score=167, Evalue=9e-42,
Organism=Homo sapiens, GI36796743, Length=241, Percent_Identity=27.3858921161826, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=43.3691756272401, Blast_Score=211, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI17568735, Length=258, Percent_Identity=39.5348837209302, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6319558, Length=269, Percent_Identity=42.7509293680297, Blast_Score=196, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6321643, Length=301, Percent_Identity=37.2093023255814, Blast_Score=177, Evalue=2e-45,
Organism=Drosophila melanogaster, GI17136816, Length=295, Percent_Identity=42.3728813559322, Blast_Score=222, Evalue=3e-58,
Organism=Drosophila melanogaster, GI17136818, Length=295, Percent_Identity=42.3728813559322, Blast_Score=221, Evalue=3e-58,
Organism=Drosophila melanogaster, GI62472483, Length=283, Percent_Identity=39.9293286219081, Blast_Score=184, Evalue=5e-47,
Organism=Drosophila melanogaster, GI45551871, Length=283, Percent_Identity=39.9293286219081, Blast_Score=184, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24645718, Length=283, Percent_Identity=39.9293286219081, Blast_Score=184, Evalue=6e-47,
Organism=Drosophila melanogaster, GI17137370, Length=283, Percent_Identity=39.9293286219081, Blast_Score=184, Evalue=6e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_MYCA9 (B1MG21)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001704420.1
- ProteinModelPortal:   B1MG21
- SMR:   B1MG21
- EnsemblBacteria:   EBMYCT00000006561
- GeneID:   5966190
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_3692c
- GeneTree:   EBGT00050000016741
- HOGENOM:   HBG328751
- OMA:   CTPLGCI
- BioCyc:   MABS561007:MAB_3692C-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 29721; Mature: 29590

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATRLDGKATRDEIFVELATRAAALKAAGKTPGLGTILVGDDPGSHAYVRGKHADCAKV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEECCCCCHHHH
GLNSIRRDLPADIDQATLCATIDELNANPDCTGYIVQLPLPKHLDENEALERIDPDKDAD
HHHHHHHHCCCCCCCHHHHEEHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCC
GLHPTNLGRLVLGKEAPLPCTPRGIVYLLRRYDVKLDGAHVVVIGRGVTVGRPLGLLLTR
CCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCEEECCEEEEEEECCCCCCCCCEEEEEE
RSENATVTLCHTGTRDLAALTRQADIIVAAAGVPHMVTADMVKPGAAVVDVGVSRVDDKL
CCCCCEEEEEECCCHHHHHHHHCCCEEEEECCCCCCEEHHHCCCCCEEEECCHHHHCHHH
TGDVAEDVWDVAGYVSPNPGGVGPLTRAFLLTNVIERAERS
CCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TATRLDGKATRDEIFVELATRAAALKAAGKTPGLGTILVGDDPGSHAYVRGKHADCAKV
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEECCCCCHHHH
GLNSIRRDLPADIDQATLCATIDELNANPDCTGYIVQLPLPKHLDENEALERIDPDKDAD
HHHHHHHHCCCCCCCHHHHEEHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCC
GLHPTNLGRLVLGKEAPLPCTPRGIVYLLRRYDVKLDGAHVVVIGRGVTVGRPLGLLLTR
CCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCEEECCEEEEEEECCCCCCCCCEEEEEE
RSENATVTLCHTGTRDLAALTRQADIIVAAAGVPHMVTADMVKPGAAVVDVGVSRVDDKL
CCCCCEEEEEECCCHHHHHHHHCCCEEEEECCCCCCEEHHHCCCCCEEEECCHHHHCHHH
TGDVAEDVWDVAGYVSPNPGGVGPLTRAFLLTNVIERAERS
CCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA