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The map label for this gene is dacB [H]

Identifier: 169630760

GI number: 169630760

Start: 3734105

End: 3735394

Strand: Direct

Name: dacB [H]

Synonym: MAB_3681

Alternate gene names: 169630760

Gene position: 3734105-3735394 (Clockwise)

Preceding gene: 169630759

Following gene: 169630764

Centisome position: 73.69

GC content: 68.68

Gene sequence:

>1290_bases
ATGACCACCTGGACTCCTGCGCCCGACCGCCGCCCGTCGCCGCGGACCGCCGTGACGCGCCTCGCCGCGGCCACCGCCGC
CGGGTTGATCGTCGCCGTCCTCCCGCTGTGCGCGCCGCCGCTCGCGAGCGCCGAGCCCGGGTTCACACCGCCCGACACCA
GCGGGTGCCCCTATAAGGTCGTCACCCCGCCTGCCGTCGACACTTCCGAGGTACCCAAGGCCGGCGGCGACCCGCCCGCT
CCACTGCCGGTGCCGGCCAAGCCCATCGGCGGCGAGATGCTCGGACGCTGCGATGTGATCACCCCGGACGGGGCCGGGCC
CGTGCCCAACGACATCTCCGCGGAATCATGGCTCATCGCCGATCTGAACACCGGGAATGTGGTGGCCGCCAAGGATCCCC
ACGCCCGCCATCGCCCCGCCAGCGTCATCAAGGTGCTGGTGGCCATGGAAGCCATCAACAACCTGAACCTCAATCAGGCA
GTGGTAGGCACCCAAGAGGACGCGAACAGCGAGGGCACCCGGGTCGGCGTCGATGTCGGCGGCACCTACACGGTCCGCCA
GCTCCTGACCGGTCTGCTGATGAACTCGGGCAACGACGCCGCGCACGCACTAGCGGTTCAGCTCGGCGGAATGGATGAGA
CCGTCGCCAAGATCAACGACATGGCGCAGAAACTCGGCGCACGAGATACCCGGGCGGCCACCCCCTCAGGTCTCGACGGC
CCCGGCATGAGCACCTCGGCCTACGACCTCGGCCTGTTCTACAAATACGCGTTCGCCGACCCCACCTTCGCCGACCTGGT
CTCTGCGCCCAAGGCCACGTTCCCCGGCCACCCCGCCAAACCCGGTGAACGCGATGACCATCCGGCCTATGAGATGACGA
ACGACAACAAGCTGCTGTACAACTACCCGGGCGCGCTCGGCGGCAAGACCGGCTACACCGACGACGCCCAGCAGACCTTC
GTCGGCGCCGCCGAACGTGATGGCCGCCGGTTGGTGGTCACCATGCTGCGCGGCACTCGGCTGCCGATCGCACCGTGGGA
GCAGGCGGCACACCTTCTCGACTATGGGTTCTCCACGCCGCGCGACGCCTCGATCGGCAAGCTCGTCGATCCAGATCCGT
CACTGTTGACCAAACCGCATGCCGACGACACCCCCTCGGCGCAGGCCGCGGGTCTGCCCGCCGAGGCGATCAACAGCGTC
CCGGTCCGGGTGGGCGTGACGATCATCGGTGCGATGATCGTGTTCGGACTCATCCTCGCGGCGCGCTCACTCAACCGCCG
ACAGGCTTAG

Upstream 100 bases:

>100_bases
TGGCGGATATCGCGTCGTCCAGCAGCGGCCCATAAGTCACATCCGACACAGGCGTAACATCCCCCAGTTCAGAGTGGTGT
TCGCTTACTGTGGCAAGGCG

Downstream 100 bases:

>100_bases
TCGCCGCCGCGCCCCAACCAGGAAATCCCCAGCGCGCCGATGACTCCCGCCCCGAAGGCGGCGATCACTCCCAGCTTGGA
TGGCCGCTCGACCGTCTCGA

Product: penicillin-binding protein DacB1

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTTWTPAPDRRPSPRTAVTRLAAATAAGLIVAVLPLCAPPLASAEPGFTPPDTSGCPYKVVTPPAVDTSEVPKAGGDPPA
PLPVPAKPIGGEMLGRCDVITPDGAGPVPNDISAESWLIADLNTGNVVAAKDPHARHRPASVIKVLVAMEAINNLNLNQA
VVGTQEDANSEGTRVGVDVGGTYTVRQLLTGLLMNSGNDAAHALAVQLGGMDETVAKINDMAQKLGARDTRAATPSGLDG
PGMSTSAYDLGLFYKYAFADPTFADLVSAPKATFPGHPAKPGERDDHPAYEMTNDNKLLYNYPGALGGKTGYTDDAQQTF
VGAAERDGRRLVVTMLRGTRLPIAPWEQAAHLLDYGFSTPRDASIGKLVDPDPSLLTKPHADDTPSAQAAGLPAEAINSV
PVRVGVTIIGAMIVFGLILAARSLNRRQA

Sequences:

>Translated_429_residues
MTTWTPAPDRRPSPRTAVTRLAAATAAGLIVAVLPLCAPPLASAEPGFTPPDTSGCPYKVVTPPAVDTSEVPKAGGDPPA
PLPVPAKPIGGEMLGRCDVITPDGAGPVPNDISAESWLIADLNTGNVVAAKDPHARHRPASVIKVLVAMEAINNLNLNQA
VVGTQEDANSEGTRVGVDVGGTYTVRQLLTGLLMNSGNDAAHALAVQLGGMDETVAKINDMAQKLGARDTRAATPSGLDG
PGMSTSAYDLGLFYKYAFADPTFADLVSAPKATFPGHPAKPGERDDHPAYEMTNDNKLLYNYPGALGGKTGYTDDAQQTF
VGAAERDGRRLVVTMLRGTRLPIAPWEQAAHLLDYGFSTPRDASIGKLVDPDPSLLTKPHADDTPSAQAAGLPAEAINSV
PVRVGVTIIGAMIVFGLILAARSLNRRQA
>Mature_428_residues
TTWTPAPDRRPSPRTAVTRLAAATAAGLIVAVLPLCAPPLASAEPGFTPPDTSGCPYKVVTPPAVDTSEVPKAGGDPPAP
LPVPAKPIGGEMLGRCDVITPDGAGPVPNDISAESWLIADLNTGNVVAAKDPHARHRPASVIKVLVAMEAINNLNLNQAV
VGTQEDANSEGTRVGVDVGGTYTVRQLLTGLLMNSGNDAAHALAVQLGGMDETVAKINDMAQKLGARDTRAATPSGLDGP
GMSTSAYDLGLFYKYAFADPTFADLVSAPKATFPGHPAKPGERDDHPAYEMTNDNKLLYNYPGALGGKTGYTDDAQQTFV
GAAERDGRRLVVTMLRGTRLPIAPWEQAAHLLDYGFSTPRDASIGKLVDPDPSLLTKPHADDTPSAQAAGLPAEAINSVP
VRVGVTIIGAMIVFGLILAARSLNRRQA

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=280, Percent_Identity=31.0714285714286, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1786851, Length=260, Percent_Identity=28.8461538461538, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787062, Length=287, Percent_Identity=26.1324041811847, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 44605; Mature: 44474

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTWTPAPDRRPSPRTAVTRLAAATAAGLIVAVLPLCAPPLASAEPGFTPPDTSGCPYKV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEE
VTPPAVDTSEVPKAGGDPPAPLPVPAKPIGGEMLGRCDVITPDGAGPVPNDISAESWLIA
ECCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCEEECCCCCCCCCCCCCCCCEEEE
DLNTGNVVAAKDPHARHRPASVIKVLVAMEAINNLNLNQAVVGTQEDANSEGTRVGVDVG
ECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEECC
GTYTVRQLLTGLLMNSGNDAAHALAVQLGGMDETVAKINDMAQKLGARDTRAATPSGLDG
CHHHHHHHHHHHHHCCCCCHHEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PGMSTSAYDLGLFYKYAFADPTFADLVSAPKATFPGHPAKPGERDDHPAYEMTNDNKLLY
CCCCCCHHHHHHEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEE
NYPGALGGKTGYTDDAQQTFVGAAERDGRRLVVTMLRGTRLPIAPWEQAAHLLDYGFSTP
ECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCC
RDASIGKLVDPDPSLLTKPHADDTPSAQAAGLPAEAINSVPVRVGVTIIGAMIVFGLILA
CCCCCCCCCCCCHHHCCCCCCCCCCCCHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHH
ARSLNRRQA
HHHHHHCCC
>Mature Secondary Structure 
TTWTPAPDRRPSPRTAVTRLAAATAAGLIVAVLPLCAPPLASAEPGFTPPDTSGCPYKV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEE
VTPPAVDTSEVPKAGGDPPAPLPVPAKPIGGEMLGRCDVITPDGAGPVPNDISAESWLIA
ECCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCEEECCCCCCCCCCCCCCCCEEEE
DLNTGNVVAAKDPHARHRPASVIKVLVAMEAINNLNLNQAVVGTQEDANSEGTRVGVDVG
ECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEECC
GTYTVRQLLTGLLMNSGNDAAHALAVQLGGMDETVAKINDMAQKLGARDTRAATPSGLDG
CHHHHHHHHHHHHHCCCCCHHEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PGMSTSAYDLGLFYKYAFADPTFADLVSAPKATFPGHPAKPGERDDHPAYEMTNDNKLLY
CCCCCCHHHHHHEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEE
NYPGALGGKTGYTDDAQQTFVGAAERDGRRLVVTMLRGTRLPIAPWEQAAHLLDYGFSTP
ECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCC
RDASIGKLVDPDPSLLTKPHADDTPSAQAAGLPAEAINSVPVRVGVTIIGAMIVFGLILA
CCCCCCCCCCCCHHHCCCCCCCCCCCCHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHH
ARSLNRRQA
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]