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The map label for this gene is lpdA [H]

Identifier: 169630735

GI number: 169630735

Start: 3708900

End: 3710315

Strand: Reverse

Name: lpdA [H]

Synonym: MAB_3656c

Alternate gene names: 169630735

Gene position: 3710315-3708900 (Counterclockwise)

Preceding gene: 169630737

Following gene: 169630734

Centisome position: 73.22

GC content: 67.51

Gene sequence:

>1416_bases
GTGGCTACGCGCATCGTGATCATTGGCGGAGGACCAGCGGGATACGAGGCGGCACTCGTGGCCGCGGCTCATGAGCGAAG
CACCACCGAGGTGACGGTGATCGACTCGGACGGGATCGGTGGCGCGTGCGTGCTGTTCGACTGCGTGCCGTCCAAGACGT
TCATCGCCTCGACGGGTGTGCGCACCGACCTGCGGCGCGCGCCCAACCTGGGCTTTGACATCGATGTGGAGCACGCCAAG
ATTTCGCTGCCGCAGATCCACAGTCGCGTCAAGCGCCTCGCCTCCGAGCAGTCCGCGGATATCGCCGAGCGGCTGCGCAG
CGCCGGGGTACACCTGATCGCCGGGCGCGCGGAACTGGTCGATCACGTGGTCGGGATGGCATTTCACCGGGTCAAGGCAA
CCGCGCCGGATGGCACCAGCACCATCCTGGAGGCCGATGTGGTGCTCATCGCCACAGGTGCCAGTCCACGTGTGTTGCCC
CACGCCCGCCCCGATGGTGAGCGCATTCTCACCTGGCGCCAGCTCTACGACCTGGATGAACTGCCCGAACATCTCATCGT
GGTGGGCTCGGGTGTCACGGGCGCCGAGTTCGTGCACGCCTACACCGAGCTTGGGGTTCAGGTCACCGTCGTGGCCAGCC
GCGATCGCGTGTTGCCGCACGAGGACGAGGATGCTGCCCTGGTCCTGGAAGACACGCTGGCCGAGCGCGGTGTGACGCTC
GTCAAGAACGCCCGGGCCGACTCGGTTGCGAGAACGGACACCGGCGTGCGGGTCTCCATGGCCGACGGCCGGGTGGTCGA
CGGCAGCCATGTGCTGATGACGGTCGGATCCATTCCGAACACCGCGGGCCTGGGCCTGGAGCGGGTGGGCATCACGCTCG
GCCCCGGCGGTTATCTCACCGTCGATCGGGTGTCGCGCACCTCGGTGGCCGGAATCTACGCCGCGGGTGACTGCACGGGG
CTGCTGCCGCTGGCATCGGTCGCTGCCATGCAGGGCCGCATCGCCATGTATCACGCGCTCGGCGACGCGGTCCAGCCCAT
TAGGTTGAAAACCGTTGCTGCCGCAGTATTTACCCGTCCGGAGATCGCCGCCGTGGGAGTCTCCCAGGCCGCCATCGACA
ACGGCGACGTGCCCGCCCGCACGGTCACTCTTCCGCTGCACACCAACCCGCGGGCCAAGATGTCGGGCCTGCGGCGCGGT
TTCGTGAAGATCTTCTGCCGCCCGGCCACCGGTGTGGTGATCGGCGGGGTGGTGGTGGCCGCGAATGCCTCCGAGCTGAT
CCTGCCCATCGCCATCGCGGTGCAGAATCTGCTTTCGGTGAATGACCTCGCGCAGACCCTGTCGGTCTATCCGTCCTTGT
CGGGATCGGTGACGGAGGCGGCGCGCCAGCTGATCGCCCACGACGACCTGGACTGA

Upstream 100 bases:

>100_bases
AACTCTAGTGGTCGTCGATCCGAGGCGATGAGCCACTCGGGCTAAGAACCTGCTCTCGGGATGGCGAGCCGGGGCCTGGG
AGTGCTTGTAGGTTATTTGC

Downstream 100 bases:

>100_bases
CCTTCGGCGATTTGCGCCACAGCGGCGTGCATCACTGACGGGAGCGCTACCGGGCCGGTAACGTGGCTGTCTGGAAGGAA
CTCCGGGGCCCCGATAGAGG

Product: flavoprotein disulfide reductase

Products: NA

Alternate protein names: NAD(P)H quinone reductase; NAD(P)H: menadione oxidoreductase; NADH-menadione reductase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MATRIVIIGGGPAGYEAALVAAAHERSTTEVTVIDSDGIGGACVLFDCVPSKTFIASTGVRTDLRRAPNLGFDIDVEHAK
ISLPQIHSRVKRLASEQSADIAERLRSAGVHLIAGRAELVDHVVGMAFHRVKATAPDGTSTILEADVVLIATGASPRVLP
HARPDGERILTWRQLYDLDELPEHLIVVGSGVTGAEFVHAYTELGVQVTVVASRDRVLPHEDEDAALVLEDTLAERGVTL
VKNARADSVARTDTGVRVSMADGRVVDGSHVLMTVGSIPNTAGLGLERVGITLGPGGYLTVDRVSRTSVAGIYAAGDCTG
LLPLASVAAMQGRIAMYHALGDAVQPIRLKTVAAAVFTRPEIAAVGVSQAAIDNGDVPARTVTLPLHTNPRAKMSGLRRG
FVKIFCRPATGVVIGGVVVAANASELILPIAIAVQNLLSVNDLAQTLSVYPSLSGSVTEAARQLIAHDDLD

Sequences:

>Translated_471_residues
MATRIVIIGGGPAGYEAALVAAAHERSTTEVTVIDSDGIGGACVLFDCVPSKTFIASTGVRTDLRRAPNLGFDIDVEHAK
ISLPQIHSRVKRLASEQSADIAERLRSAGVHLIAGRAELVDHVVGMAFHRVKATAPDGTSTILEADVVLIATGASPRVLP
HARPDGERILTWRQLYDLDELPEHLIVVGSGVTGAEFVHAYTELGVQVTVVASRDRVLPHEDEDAALVLEDTLAERGVTL
VKNARADSVARTDTGVRVSMADGRVVDGSHVLMTVGSIPNTAGLGLERVGITLGPGGYLTVDRVSRTSVAGIYAAGDCTG
LLPLASVAAMQGRIAMYHALGDAVQPIRLKTVAAAVFTRPEIAAVGVSQAAIDNGDVPARTVTLPLHTNPRAKMSGLRRG
FVKIFCRPATGVVIGGVVVAANASELILPIAIAVQNLLSVNDLAQTLSVYPSLSGSVTEAARQLIAHDDLD
>Mature_470_residues
ATRIVIIGGGPAGYEAALVAAAHERSTTEVTVIDSDGIGGACVLFDCVPSKTFIASTGVRTDLRRAPNLGFDIDVEHAKI
SLPQIHSRVKRLASEQSADIAERLRSAGVHLIAGRAELVDHVVGMAFHRVKATAPDGTSTILEADVVLIATGASPRVLPH
ARPDGERILTWRQLYDLDELPEHLIVVGSGVTGAEFVHAYTELGVQVTVVASRDRVLPHEDEDAALVLEDTLAERGVTLV
KNARADSVARTDTGVRVSMADGRVVDGSHVLMTVGSIPNTAGLGLERVGITLGPGGYLTVDRVSRTSVAGIYAAGDCTGL
LPLASVAAMQGRIAMYHALGDAVQPIRLKTVAAAVFTRPEIAAVGVSQAAIDNGDVPARTVTLPLHTNPRAKMSGLRRGF
VKIFCRPATGVVIGGVVVAANASELILPIAIAVQNLLSVNDLAQTLSVYPSLSGSVTEAARQLIAHDDLD

Specific function: May contribute to virulence by increasing resistance to reactive oxygen intermediates. It can reduce 2,6-dimethyl-1,4- benzoquinone (DMBQ), 5-hydroxy-1,4-naphthaquinone (5-HNQ) and menadione. NAD(P)H is the physiological reductant rather than NADH [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=471, Percent_Identity=27.3885350318471, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI50301238, Length=442, Percent_Identity=25.7918552036199, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI22035672, Length=437, Percent_Identity=25.629290617849, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI291045266, Length=435, Percent_Identity=24.367816091954, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI33519430, Length=433, Percent_Identity=24.9422632794457, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI33519428, Length=433, Percent_Identity=24.9422632794457, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI33519426, Length=433, Percent_Identity=24.9422632794457, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI148277065, Length=433, Percent_Identity=24.9422632794457, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI148277071, Length=433, Percent_Identity=24.9422632794457, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI291045268, Length=323, Percent_Identity=24.7678018575851, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1786307, Length=468, Percent_Identity=26.4957264957265, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI87082354, Length=426, Percent_Identity=24.1784037558685, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI87081717, Length=464, Percent_Identity=26.5086206896552, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789915, Length=440, Percent_Identity=23.1818181818182, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI32565766, Length=466, Percent_Identity=27.0386266094421, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17557007, Length=472, Percent_Identity=25.635593220339, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71983419, Length=435, Percent_Identity=25.7471264367816, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71983429, Length=435, Percent_Identity=25.7471264367816, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321091, Length=481, Percent_Identity=25.5717255717256, Blast_Score=143, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6325166, Length=443, Percent_Identity=24.3792325056433, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6325240, Length=484, Percent_Identity=22.3140495867769, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21358499, Length=469, Percent_Identity=27.9317697228145, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24640549, Length=481, Percent_Identity=27.8586278586279, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24640553, Length=481, Percent_Identity=27.8586278586279, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24640551, Length=489, Percent_Identity=27.19836400818, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI17737741, Length=493, Percent_Identity=24.9492900608519, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.6.5.2 [H]

Molecular weight: Translated: 49225; Mature: 49094

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRIVIIGGGPAGYEAALVAAAHERSTTEVTVIDSDGIGGACVLFDCVPSKTFIASTGV
CCEEEEEECCCCCCCHHEEEEECCCCCCEEEEEEECCCCCCEEEEEEECCCCEEEECCCC
RTDLRRAPNLGFDIDVEHAKISLPQIHSRVKRLASEQSADIAERLRSAGVHLIAGRAELV
HHHHHHCCCCCCEEECCHHEECCHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEECHHHHH
DHVVGMAFHRVKATAPDGTSTILEADVVLIATGASPRVLPHARPDGERILTWRQLYDLDE
HHHHHHHHHHEEECCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCHHEEHHHHHCCHHH
LPEHLIVVGSGVTGAEFVHAYTELGVQVTVVASRDRVLPHEDEDAALVLEDTLAERGVTL
CCCEEEEEECCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCEEEEEHHHHHCCCEE
VKNARADSVARTDTGVRVSMADGRVVDGSHVLMTVGSIPNTAGLGLERVGITLGPGGYLT
EECCCCCCCEECCCCEEEEECCCEEECCCEEEEEECCCCCCCCCCEEEEEEEECCCCEEE
VDRVSRTSVAGIYAAGDCTGLLPLASVAAMQGRIAMYHALGDAVQPIRLKTVAAAVFTRP
EECCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEHHCCCCCCCHHHHHHHHHHCCC
EIAAVGVSQAAIDNGDVPARTVTLPLHTNPRAKMSGLRRGFVKIFCRPATGVVIGGVVVA
CEEEECCHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHCCEEEEEECCCCCEEEEEEEEE
ANASELILPIAIAVQNLLSVNDLAQTLSVYPSLSGSVTEAARQLIAHDDLD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ATRIVIIGGGPAGYEAALVAAAHERSTTEVTVIDSDGIGGACVLFDCVPSKTFIASTGV
CEEEEEECCCCCCCHHEEEEECCCCCCEEEEEEECCCCCCEEEEEEECCCCEEEECCCC
RTDLRRAPNLGFDIDVEHAKISLPQIHSRVKRLASEQSADIAERLRSAGVHLIAGRAELV
HHHHHHCCCCCCEEECCHHEECCHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEECHHHHH
DHVVGMAFHRVKATAPDGTSTILEADVVLIATGASPRVLPHARPDGERILTWRQLYDLDE
HHHHHHHHHHEEECCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCHHEEHHHHHCCHHH
LPEHLIVVGSGVTGAEFVHAYTELGVQVTVVASRDRVLPHEDEDAALVLEDTLAERGVTL
CCCEEEEEECCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCEEEEEHHHHHCCCEE
VKNARADSVARTDTGVRVSMADGRVVDGSHVLMTVGSIPNTAGLGLERVGITLGPGGYLT
EECCCCCCCEECCCCEEEEECCCEEECCCEEEEEECCCCCCCCCCEEEEEEEECCCCEEE
VDRVSRTSVAGIYAAGDCTGLLPLASVAAMQGRIAMYHALGDAVQPIRLKTVAAAVFTRP
EECCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEHHCCCCCCCHHHHHHHHHHCCC
EIAAVGVSQAAIDNGDVPARTVTLPLHTNPRAKMSGLRRGFVKIFCRPATGVVIGGVVVA
CEEEECCHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHCCEEEEEECCCCCEEEEEEEEE
ANASELILPIAIAVQNLLSVNDLAQTLSVYPSLSGSVTEAARQLIAHDDLD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]