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The map label for this gene is nasB [H]

Identifier: 169630603

GI number: 169630603

Start: 3567169

End: 3569682

Strand: Reverse

Name: nasB [H]

Synonym: MAB_3522c

Alternate gene names: 169630603

Gene position: 3569682-3567169 (Counterclockwise)

Preceding gene: 169630604

Following gene: 169630602

Centisome position: 70.45

GC content: 65.08

Gene sequence:

>2514_bases
ATGAACAAGAAAGTCGTCGTCATCGGCCATGGAATGGTCGGTCACCGATTTGTCCAGGTGCTGCGTGAGCGCGATGCCAC
CGATCAGTGGCAGGTGACGGTTCTGGGTGAGGAAGCCGATGCCGCCTATGACCGGGTGGCGTTGTCCTCGTACATCGACA
GCTGGGACCGGGATACGCTGGCGCTCACCGGAAACGACTACGCGGAAGACCCGATGGTGACACTTCACCTCGGCGACCCG
GTGGTGGGCATCGACCGGACCAATCAGACGGTCAGCACTGCGGCGGGCACCGTGCTGGACTATGACGCGCTTGTGATGGC
CACCGGGTCTTACCCGTTCGTGCCGCCGGTGCCCGGTGGGGACGCTGAGGGCTGCTTTGTGTACCGCACCATGGATGACC
TCGATGCCATCAAGGCCACCGCGGACAAGTCGCCCGGCTCAGCTGGTGTTGTGATCGGTGGCGGGCTACTGGGGCTGGAG
GCCGCCAATGCGCTGCGGCTCATGGGATTGGCCCCGCACGTGGTGGAGCTCAATCCCCGCCTCATGCACCTGCAGGTCGA
CGAGGGCGGCGGGGCCCTGCTGACCCGGCTGGTGACCGACCTGGGGTTGACCGTGCACACCAGCGTGTCCACACAGGCCA
TCGAGAAGGCGGCCGATGGCACACTGTCGGTGCAGCTGTCGGACGGGTCGACGATCGATGCCTCGGTGCTAGTGTTCTCG
GCCGGTATCCGGCCACGCGATGAGCTGGCCCGCGAGTGTGGACTGGAGCTGGCCGAGCGCGGCGGCATATTCACCGACCT
GGGCTGTCAGACAAGTGATCTGCACATCTACGCGATCGGTGAGGTCGCCGCGATAGAAGGCCGCTGCTACGGTCTGGTCG
CGCCCGGGTACACCACGGCGGAAATCGTCGCGGACCGGCTACTGGGCGGCAGTGCTCAGTTCCCCGGGGCCGACCTGTCC
ACCAAACTCAAACTGCTGGGGGTCGACGTCGCCAGCTTTGGAGACGCGCACGCCACCGCCGATGGTGCGCTCGAGGTCGT
TTTCAATGACGCCACCAAGGGCACCTACGCCAAGCTCGTGGTTTCCGACGATGCGCGAACTCTGTTGGGCGGCATCCTCG
TCGGTGACGCTAGTGCCTACGGGACCCTGCGGCCCATGCTCGGCAGGGAGCTGCCCGCGGATCCGGCCTCGCTCATCGCC
CCATCGGGTGCGGAGATCGGCGTGGGCGCGCTGCCCGACGATGCGCAGATCTGCTCGTGCAACGCCGTGACCAAGGGGGC
GATATGCGCAGCCATCTGTGAGGGCGCCACCGATGTGCCCGCTCTGAAGTCGGCAACCTGTGCCGGAACCTCTTGCGGTA
GCTGCATTCCCATGCTCAAGCAGATTCTTGCGGCCCAGGGCGTCGAGCAGTCCAAGGCGCTGTGTGAGCACTTCGAGCAG
TCGCGCGCCGAGCTGTTCCAGGTGGTGCAGGCGACCGGAATCCGGACCTTCTCCGAGCTCATTGCCAAGCACGGCAAGGG
CACTGGTTGCGATATCTGCAAGCCCACCGTGGCGTCCATCCTGGCCTCGACGTCCAGCGATCACATCCTGGAAGGCGAGC
AGGCCGGGCTGCAGGACACCAACGATCACTTCCTGGCCAACATGCAGAAGAACGGCACCTACTCGGTAGTGCCGCGGCTG
CCCGGCGGTGAGGTCACTCCGGAGAAGCTCATCGTCATCGGCGAGATCGCCCGGGATTTCGGTTTGTACACCAAGATCAC
CGGTGGTCAGCGCATCGATCTGTTCGGTGCTCGCGTCGAGCAGCTCCCGCTGATCTGGAAGCGGCTCATCGATGCCGGCA
TGGAATCGGGGCACGCCTACGGCAAGTCGTTGCGCACGGTGAAGAGCTGTGTCGGGTCCACCTGGTGCCGATACGGGGTT
CAGGACTCGGTCGCAATGGCCGTTGAGCTTGAATTGCGTTATCGCGGACTGCGTTCGCCGCACAAGCTCAAGATGGGCGT
ATCCGGGTGTGCTCGGGAATGTGCCGAGGCGCGTGGCAAGGACGTCGGCGTCATCGCCACCGAGAATGGCTGGAATCTCT
ACGTGGGCGGTAATGGCGGCGCGACGCCGGCACATGCCAAATTGCTTGCCGGCGACCTGGACTCGGAGACGCTGATCAGG
TACATCGATAGGTACCTGATGTTCTACATCCGCACCGCGGACAGGCTGCAGCGCACCGCGCCCTGGCAGGAGGCCATCGA
GGGCGGCCTGGATCACATCCGGGCGGTCGTGTGCGAGGACTCGCTGGGCATCGCCGACGAGCTGGAAGCGGCGATGGCTC
GTCACGTGCAGGGCTACCAGGACGAATGGGCAGCGGTCCTGGCCGACCCGGACAAGCTGCGACGGTTTGTTTCCTTCGTC
AACGCGCCCGATCAGCCCGATTCGACCATCGCATTCGACGAAAGTGGCCCGCGCAAGGTGCCGGTGCTGCTGGGTACGCC
CGGTTTCCGGGCCGCCGCCGAGGCCGCAACATAA

Upstream 100 bases:

>100_bases
TCTGCGCGGCGGTCACCTGGTACGCGTACGTGCGGCGGCCCATGACCGTATCGGTTCCGGCGATCTCCAGCTCCAAAAAC
GAAGTGGCGGTGTGAAACCC

Downstream 100 bases:

>100_bases
CGCATCGGCCCCGGTAATACCGGGGTAACACAACGTCGGTACACATGACATATCGCGTGAAAACGCAGGAAACAACGAGA
AGAAAGGCCGGAGATGACAA

Product: nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 837; Mature: 837

Protein sequence:

>837_residues
MNKKVVVIGHGMVGHRFVQVLRERDATDQWQVTVLGEEADAAYDRVALSSYIDSWDRDTLALTGNDYAEDPMVTLHLGDP
VVGIDRTNQTVSTAAGTVLDYDALVMATGSYPFVPPVPGGDAEGCFVYRTMDDLDAIKATADKSPGSAGVVIGGGLLGLE
AANALRLMGLAPHVVELNPRLMHLQVDEGGGALLTRLVTDLGLTVHTSVSTQAIEKAADGTLSVQLSDGSTIDASVLVFS
AGIRPRDELARECGLELAERGGIFTDLGCQTSDLHIYAIGEVAAIEGRCYGLVAPGYTTAEIVADRLLGGSAQFPGADLS
TKLKLLGVDVASFGDAHATADGALEVVFNDATKGTYAKLVVSDDARTLLGGILVGDASAYGTLRPMLGRELPADPASLIA
PSGAEIGVGALPDDAQICSCNAVTKGAICAAICEGATDVPALKSATCAGTSCGSCIPMLKQILAAQGVEQSKALCEHFEQ
SRAELFQVVQATGIRTFSELIAKHGKGTGCDICKPTVASILASTSSDHILEGEQAGLQDTNDHFLANMQKNGTYSVVPRL
PGGEVTPEKLIVIGEIARDFGLYTKITGGQRIDLFGARVEQLPLIWKRLIDAGMESGHAYGKSLRTVKSCVGSTWCRYGV
QDSVAMAVELELRYRGLRSPHKLKMGVSGCARECAEARGKDVGVIATENGWNLYVGGNGGATPAHAKLLAGDLDSETLIR
YIDRYLMFYIRTADRLQRTAPWQEAIEGGLDHIRAVVCEDSLGIADELEAAMARHVQGYQDEWAAVLADPDKLRRFVSFV
NAPDQPDSTIAFDESGPRKVPVLLGTPGFRAAAEAAT

Sequences:

>Translated_837_residues
MNKKVVVIGHGMVGHRFVQVLRERDATDQWQVTVLGEEADAAYDRVALSSYIDSWDRDTLALTGNDYAEDPMVTLHLGDP
VVGIDRTNQTVSTAAGTVLDYDALVMATGSYPFVPPVPGGDAEGCFVYRTMDDLDAIKATADKSPGSAGVVIGGGLLGLE
AANALRLMGLAPHVVELNPRLMHLQVDEGGGALLTRLVTDLGLTVHTSVSTQAIEKAADGTLSVQLSDGSTIDASVLVFS
AGIRPRDELARECGLELAERGGIFTDLGCQTSDLHIYAIGEVAAIEGRCYGLVAPGYTTAEIVADRLLGGSAQFPGADLS
TKLKLLGVDVASFGDAHATADGALEVVFNDATKGTYAKLVVSDDARTLLGGILVGDASAYGTLRPMLGRELPADPASLIA
PSGAEIGVGALPDDAQICSCNAVTKGAICAAICEGATDVPALKSATCAGTSCGSCIPMLKQILAAQGVEQSKALCEHFEQ
SRAELFQVVQATGIRTFSELIAKHGKGTGCDICKPTVASILASTSSDHILEGEQAGLQDTNDHFLANMQKNGTYSVVPRL
PGGEVTPEKLIVIGEIARDFGLYTKITGGQRIDLFGARVEQLPLIWKRLIDAGMESGHAYGKSLRTVKSCVGSTWCRYGV
QDSVAMAVELELRYRGLRSPHKLKMGVSGCARECAEARGKDVGVIATENGWNLYVGGNGGATPAHAKLLAGDLDSETLIR
YIDRYLMFYIRTADRLQRTAPWQEAIEGGLDHIRAVVCEDSLGIADELEAAMARHVQGYQDEWAAVLADPDKLRRFVSFV
NAPDQPDSTIAFDESGPRKVPVLLGTPGFRAAAEAAT
>Mature_837_residues
MNKKVVVIGHGMVGHRFVQVLRERDATDQWQVTVLGEEADAAYDRVALSSYIDSWDRDTLALTGNDYAEDPMVTLHLGDP
VVGIDRTNQTVSTAAGTVLDYDALVMATGSYPFVPPVPGGDAEGCFVYRTMDDLDAIKATADKSPGSAGVVIGGGLLGLE
AANALRLMGLAPHVVELNPRLMHLQVDEGGGALLTRLVTDLGLTVHTSVSTQAIEKAADGTLSVQLSDGSTIDASVLVFS
AGIRPRDELARECGLELAERGGIFTDLGCQTSDLHIYAIGEVAAIEGRCYGLVAPGYTTAEIVADRLLGGSAQFPGADLS
TKLKLLGVDVASFGDAHATADGALEVVFNDATKGTYAKLVVSDDARTLLGGILVGDASAYGTLRPMLGRELPADPASLIA
PSGAEIGVGALPDDAQICSCNAVTKGAICAAICEGATDVPALKSATCAGTSCGSCIPMLKQILAAQGVEQSKALCEHFEQ
SRAELFQVVQATGIRTFSELIAKHGKGTGCDICKPTVASILASTSSDHILEGEQAGLQDTNDHFLANMQKNGTYSVVPRL
PGGEVTPEKLIVIGEIARDFGLYTKITGGQRIDLFGARVEQLPLIWKRLIDAGMESGHAYGKSLRTVKSCVGSTWCRYGV
QDSVAMAVELELRYRGLRSPHKLKMGVSGCARECAEARGKDVGVIATENGWNLYVGGNGGATPAHAKLLAGDLDSETLIR
YIDRYLMFYIRTADRLQRTAPWQEAIEGGLDHIRAVVCEDSLGIADELEAAMARHVQGYQDEWAAVLADPDKLRRFVSFV
NAPDQPDSTIAFDESGPRKVPVLLGTPGFRAAAEAAT

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI226437568, Length=277, Percent_Identity=25.2707581227437, Blast_Score=75, Evalue=3e-13,
Organism=Homo sapiens, GI65787454, Length=277, Percent_Identity=25.2707581227437, Blast_Score=75, Evalue=3e-13,
Organism=Homo sapiens, GI21389617, Length=277, Percent_Identity=25.2707581227437, Blast_Score=75, Evalue=3e-13,
Organism=Escherichia coli, GI1789765, Length=819, Percent_Identity=52.014652014652, Blast_Score=850, Evalue=0.0,
Organism=Escherichia coli, GI1789065, Length=393, Percent_Identity=26.7175572519084, Blast_Score=105, Evalue=2e-23,
Organism=Escherichia coli, GI1788892, Length=284, Percent_Identity=27.112676056338, Blast_Score=79, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17559934, Length=283, Percent_Identity=24.7349823321555, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24585130, Length=282, Percent_Identity=22.6950354609929, Blast_Score=72, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR012748
- InterPro:   IPR017881
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327
- InterPro:   IPR017941 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 88292; Mature: 88292

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00022 EGF_1 ; PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKVVVIGHGMVGHRFVQVLRERDATDQWQVTVLGEEADAAYDRVALSSYIDSWDRDTL
CCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEE
ALTGNDYAEDPMVTLHLGDPVVGIDRTNQTVSTAAGTVLDYDALVMATGSYPFVPPVPGG
EECCCCCCCCCEEEEECCCCEEECCCCCCCHHHHCCCEEECCEEEEECCCCCCCCCCCCC
DAEGCFVYRTMDDLDAIKATADKSPGSAGVVIGGGLLGLEAANALRLMGLAPHVVELNPR
CCCCEEEEEEHHHHHHHHHHCCCCCCCCEEEEECCEEHHHHHHHHHHHCCCCCEEECCCE
LMHLQVDEGGGALLTRLVTDLGLTVHTSVSTQAIEKAADGTLSVQLSDGSTIDASVLVFS
EEEEEECCCCHHHHHHHHHHCCCEEEECCHHHHHHHHCCCEEEEEECCCCCCEEEEEEEE
AGIRPRDELARECGLELAERGGIFTDLGCQTSDLHIYAIGEVAAIEGRCYGLVAPGYTTA
CCCCCHHHHHHHHCHHHHHCCCEEEECCCCCCCEEEEEECCEEEECCCEEEEECCCCCHH
EIVADRLLGGSAQFPGADLSTKLKLLGVDVASFGDAHATADGALEVVFNDATKGTYAKLV
HHHHHHHCCCCCCCCCCCCCCEEEEEEEEHHHCCCCCCCCCCEEEEEEECCCCCEEEEEE
VSDDARTLLGGILVGDASAYGTLRPMLGRELPADPASLIAPSGAEIGVGALPDDAQICSC
ECCCHHHHHCEEEEECCCCCCCHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEC
NAVTKGAICAAICEGATDVPALKSATCAGTSCGSCIPMLKQILAAQGVEQSKALCEHFEQ
CCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
SRAELFQVVQATGIRTFSELIAKHGKGTGCDICKPTVASILASTSSDHILEGEQAGLQDT
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCEECCCCCCCCCC
NDHFLANMQKNGTYSVVPRLPGGEVTPEKLIVIGEIARDFGLYTKITGGQRIDLFGARVE
CCHHEEEECCCCCEEEEECCCCCCCCHHHEEEEEHHHHHCCCEEEECCCCEEEEECCCHH
QLPLIWKRLIDAGMESGHAYGKSLRTVKSCVGSTWCRYGVQDSVAMAVELELRYRGLRSP
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEHHCCCCCC
HKLKMGVSGCARECAEARGKDVGVIATENGWNLYVGGNGGATPAHAKLLAGDLDSETLIR
HHEECCHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCCCHHHHHEECCCCHHHHHH
YIDRYLMFYIRTADRLQRTAPWQEAIEGGLDHIRAVVCEDSLGIADELEAAMARHVQGYQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHEEECCCCCCHHHHHHHHHHHHCCCC
DEWAAVLADPDKLRRFVSFVNAPDQPDSTIAFDESGPRKVPVLLGTPGFRAAAEAAT
CCHHEEECCHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEEEECCCCCCHHCCCC
>Mature Secondary Structure
MNKKVVVIGHGMVGHRFVQVLRERDATDQWQVTVLGEEADAAYDRVALSSYIDSWDRDTL
CCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEE
ALTGNDYAEDPMVTLHLGDPVVGIDRTNQTVSTAAGTVLDYDALVMATGSYPFVPPVPGG
EECCCCCCCCCEEEEECCCCEEECCCCCCCHHHHCCCEEECCEEEEECCCCCCCCCCCCC
DAEGCFVYRTMDDLDAIKATADKSPGSAGVVIGGGLLGLEAANALRLMGLAPHVVELNPR
CCCCEEEEEEHHHHHHHHHHCCCCCCCCEEEEECCEEHHHHHHHHHHHCCCCCEEECCCE
LMHLQVDEGGGALLTRLVTDLGLTVHTSVSTQAIEKAADGTLSVQLSDGSTIDASVLVFS
EEEEEECCCCHHHHHHHHHHCCCEEEECCHHHHHHHHCCCEEEEEECCCCCCEEEEEEEE
AGIRPRDELARECGLELAERGGIFTDLGCQTSDLHIYAIGEVAAIEGRCYGLVAPGYTTA
CCCCCHHHHHHHHCHHHHHCCCEEEECCCCCCCEEEEEECCEEEECCCEEEEECCCCCHH
EIVADRLLGGSAQFPGADLSTKLKLLGVDVASFGDAHATADGALEVVFNDATKGTYAKLV
HHHHHHHCCCCCCCCCCCCCCEEEEEEEEHHHCCCCCCCCCCEEEEEEECCCCCEEEEEE
VSDDARTLLGGILVGDASAYGTLRPMLGRELPADPASLIAPSGAEIGVGALPDDAQICSC
ECCCHHHHHCEEEEECCCCCCCHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEC
NAVTKGAICAAICEGATDVPALKSATCAGTSCGSCIPMLKQILAAQGVEQSKALCEHFEQ
CCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
SRAELFQVVQATGIRTFSELIAKHGKGTGCDICKPTVASILASTSSDHILEGEQAGLQDT
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCEECCCCCCCCCC
NDHFLANMQKNGTYSVVPRLPGGEVTPEKLIVIGEIARDFGLYTKITGGQRIDLFGARVE
CCHHEEEECCCCCEEEEECCCCCCCCHHHEEEEEHHHHHCCCEEEECCCCEEEEECCCHH
QLPLIWKRLIDAGMESGHAYGKSLRTVKSCVGSTWCRYGVQDSVAMAVELELRYRGLRSP
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEHHCCCCCC
HKLKMGVSGCARECAEARGKDVGVIATENGWNLYVGGNGGATPAHAKLLAGDLDSETLIR
HHEECCHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCCCHHHHHEECCCCHHHHHH
YIDRYLMFYIRTADRLQRTAPWQEAIEGGLDHIRAVVCEDSLGIADELEAAMARHVQGYQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHEEECCCCCCHHHHHHHHHHHHCCCC
DEWAAVLADPDKLRRFVSFVNAPDQPDSTIAFDESGPRKVPVLLGTPGFRAAAEAAT
CCHHEEECCHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEEEECCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8468296 [H]