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The map label for this gene is fadA [H]

Identifier: 169630536

GI number: 169630536

Start: 3500096

End: 3501289

Strand: Reverse

Name: fadA [H]

Synonym: MAB_3455c

Alternate gene names: 169630536

Gene position: 3501289-3500096 (Counterclockwise)

Preceding gene: 169630539

Following gene: 169630534

Centisome position: 69.1

GC content: 63.23

Gene sequence:

>1194_bases
ATGAGGGATGCGGTGATCATCGACGCGGTGCGGACACCGGTCGGCAAGGGCAAGCCAGGGGGGTCGCTCTCGGGTGTGCA
TCCGGTTGATCTGCACGCGCATGCGATTCGTGAACTCGTTGAACGCGCCGGTATTGATCCGGCACTAATCGACGACGTGA
TCAGCGGCGCGGTGGGGCAGGTGGGTGAGCAGAGCTCCAATACGGCGCGCTGGGCGGCGCTGGCCGCGGGACTGCCCGAG
ACGGTTCCGGCGGTGACCGTCGACCGTCAATGTGGCAGTAGTCAGCAGGCCATCCACTTTGCCGCGCAAGGTGTAATCGC
AGGAGCCTATGACGTCGTGATCGCCTCGGGTATCGAATCGATGAGCCGAGTGCCCATGGGAAGTCAAAGTTTGGGTAAGG
ACTTCTTCGGTTCCGAAGTAGCGCGACGATACCCGGAAGGGCTTGTCCCGCAGGGCATCAGCGCCGAGCTGATCGCCGCG
AAATGGAACCTGTCACGGGAGCAGCTGGATACGTTTGCCGCAGAGAGTCACCGTCGTGCTGCACAAGCGTGGTCGGAGGG
CAGATTTGCCCGCGATGTGGTGCCCATCAAGGCCCCCGACGTCAACGGTGACCTGGTCGAGGTGTCCACCGACGAGTCCG
TGCGGCCGTCGACCACGGTTGAGGTGCTCGCCGGACTCAAGCCGGCCTTCCGTAATGAACTCTGGCAACAGCGCTTCCCG
GGGCTTGATTGGAAGGTGACCGCGGGCAACTCGTCGCCGATCAACGATGGCGCCTCTGCTGTGCTCATCACATCCAGTGA
GACCGCTCATCGTCTCGGCCTGACGCCGCGCGCTCGCGTGCATTCGGTGGCGGTGGTGGGCGACGATCCGTTATACATGC
TGACCGGCATCATCCCGGCAACCGCGAAAGTACTCGATAGAGCAGGGTTATCGTTGGCGGACATCGATGCCTTCGAGGTC
AACGAGGCTTTCGCGTCGGTGGTGCTCGCTTGGCAGGCCGAGACCGGTGCGGACCTGTCCAAGGTAAACGTCAACGGGGG
AGCCACCGCGATCGGCCACCCGCTCGGCGCCAGTGGCGGACGGCTGATGACTACGCTGGTATCGGTGCTGGAACAGGGCG
GTGGACGGTACGGCCTGCAAACGATGTGTGAAGCAGGCGGTTTGGCTAATGCCACCATCCTTGAGCGGCTTTAG

Upstream 100 bases:

>100_bases
CGTAGCACGTTATGTGGTTGATCACAGTTAGTTGTGATTTCCAACTTACTGGGCGTATACCGAGATGAGGGAGTTCAACG
GCGAATCCAGGAGGTAGGTC

Downstream 100 bases:

>100_bases
GAATGCTGTCAGCCGGGTTACGGCAGAGTGGTGCGCATCAACATCACGTTGTACGCCGACCACAGTGACAGGTTGAAGTA
CAGCTCCTTGCCGGTGGTCC

Product: putative acyl-CoA thiolase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MRDAVIIDAVRTPVGKGKPGGSLSGVHPVDLHAHAIRELVERAGIDPALIDDVISGAVGQVGEQSSNTARWAALAAGLPE
TVPAVTVDRQCGSSQQAIHFAAQGVIAGAYDVVIASGIESMSRVPMGSQSLGKDFFGSEVARRYPEGLVPQGISAELIAA
KWNLSREQLDTFAAESHRRAAQAWSEGRFARDVVPIKAPDVNGDLVEVSTDESVRPSTTVEVLAGLKPAFRNELWQQRFP
GLDWKVTAGNSSPINDGASAVLITSSETAHRLGLTPRARVHSVAVVGDDPLYMLTGIIPATAKVLDRAGLSLADIDAFEV
NEAFASVVLAWQAETGADLSKVNVNGGATAIGHPLGASGGRLMTTLVSVLEQGGGRYGLQTMCEAGGLANATILERL

Sequences:

>Translated_397_residues
MRDAVIIDAVRTPVGKGKPGGSLSGVHPVDLHAHAIRELVERAGIDPALIDDVISGAVGQVGEQSSNTARWAALAAGLPE
TVPAVTVDRQCGSSQQAIHFAAQGVIAGAYDVVIASGIESMSRVPMGSQSLGKDFFGSEVARRYPEGLVPQGISAELIAA
KWNLSREQLDTFAAESHRRAAQAWSEGRFARDVVPIKAPDVNGDLVEVSTDESVRPSTTVEVLAGLKPAFRNELWQQRFP
GLDWKVTAGNSSPINDGASAVLITSSETAHRLGLTPRARVHSVAVVGDDPLYMLTGIIPATAKVLDRAGLSLADIDAFEV
NEAFASVVLAWQAETGADLSKVNVNGGATAIGHPLGASGGRLMTTLVSVLEQGGGRYGLQTMCEAGGLANATILERL
>Mature_397_residues
MRDAVIIDAVRTPVGKGKPGGSLSGVHPVDLHAHAIRELVERAGIDPALIDDVISGAVGQVGEQSSNTARWAALAAGLPE
TVPAVTVDRQCGSSQQAIHFAAQGVIAGAYDVVIASGIESMSRVPMGSQSLGKDFFGSEVARRYPEGLVPQGISAELIAA
KWNLSREQLDTFAAESHRRAAQAWSEGRFARDVVPIKAPDVNGDLVEVSTDESVRPSTTVEVLAGLKPAFRNELWQQRFP
GLDWKVTAGNSSPINDGASAVLITSSETAHRLGLTPRARVHSVAVVGDDPLYMLTGIIPATAKVLDRAGLSLADIDAFEV
NEAFASVVLAWQAETGADLSKVNVNGGATAIGHPLGASGGRLMTTLVSVLEQGGGRYGLQTMCEAGGLANATILERL

Specific function: Subunit Of The Multifunctional Enzyme Complex Of The Fatty Acid Degradation Cycle. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=402, Percent_Identity=37.0646766169154, Blast_Score=231, Evalue=8e-61,
Organism=Homo sapiens, GI167614485, Length=407, Percent_Identity=35.8722358722359, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI4501853, Length=400, Percent_Identity=37.5, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI4557237, Length=407, Percent_Identity=35.6265356265356, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI4504327, Length=440, Percent_Identity=35.4545454545455, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI194353979, Length=236, Percent_Identity=31.7796610169492, Blast_Score=105, Evalue=6e-23,
Organism=Escherichia coli, GI48994986, Length=401, Percent_Identity=44.139650872818, Blast_Score=301, Evalue=5e-83,
Organism=Escherichia coli, GI1787663, Length=413, Percent_Identity=43.3414043583535, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI1788554, Length=407, Percent_Identity=40.04914004914, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI87082165, Length=405, Percent_Identity=38.2716049382716, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI1788683, Length=433, Percent_Identity=32.7944572748268, Blast_Score=172, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI133906874, Length=404, Percent_Identity=35.6435643564356, Blast_Score=215, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI17535921, Length=403, Percent_Identity=33.7468982630273, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI25147385, Length=407, Percent_Identity=33.1695331695332, Blast_Score=197, Evalue=9e-51,
Organism=Caenorhabditis elegans, GI17551802, Length=445, Percent_Identity=34.1573033707865, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17535917, Length=407, Percent_Identity=30.2211302211302, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6322031, Length=399, Percent_Identity=35.5889724310777, Blast_Score=208, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6325229, Length=418, Percent_Identity=34.2105263157895, Blast_Score=199, Evalue=7e-52,
Organism=Drosophila melanogaster, GI24655093, Length=405, Percent_Identity=37.2839506172839, Blast_Score=235, Evalue=4e-62,
Organism=Drosophila melanogaster, GI24640423, Length=406, Percent_Identity=34.4827586206897, Blast_Score=208, Evalue=6e-54,
Organism=Drosophila melanogaster, GI17648125, Length=405, Percent_Identity=35.5555555555556, Blast_Score=205, Evalue=5e-53,
Organism=Drosophila melanogaster, GI17137578, Length=440, Percent_Identity=34.3181818181818, Blast_Score=196, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 41418; Mature: 41418

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDAVIIDAVRTPVGKGKPGGSLSGVHPVDLHAHAIRELVERAGIDPALIDDVISGAVGQ
CCCCEEEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VGEQSSNTARWAALAAGLPETVPAVTVDRQCGSSQQAIHFAAQGVIAGAYDVVIASGIES
CCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MSRVPMGSQSLGKDFFGSEVARRYPEGLVPQGISAELIAAKWNLSREQLDTFAAESHRRA
HHHCCCCCHHHCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
AQAWSEGRFARDVVPIKAPDVNGDLVEVSTDESVRPSTTVEVLAGLKPAFRNELWQQRFP
HHHHHCCCHHHCEECEECCCCCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHCC
GLDWKVTAGNSSPINDGASAVLITSSETAHRLGLTPRARVHSVAVVGDDPLYMLTGIIPA
CCCEEEECCCCCCCCCCCCEEEEECCCCHHHHCCCCCHHHEEEEEECCCCCEEHHHHHHH
TAKVLDRAGLSLADIDAFEVNEAFASVVLAWQAETGADLSKVNVNGGATAIGHPLGASGG
HHHHHHHCCCCEECCCHHHHHHHHHHHEEEEECCCCCCCEEEECCCCCCCCCCCCCCCCC
RLMTTLVSVLEQGGGRYGLQTMCEAGGLANATILERL
HHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHCC
>Mature Secondary Structure
MRDAVIIDAVRTPVGKGKPGGSLSGVHPVDLHAHAIRELVERAGIDPALIDDVISGAVGQ
CCCCEEEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VGEQSSNTARWAALAAGLPETVPAVTVDRQCGSSQQAIHFAAQGVIAGAYDVVIASGIES
CCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MSRVPMGSQSLGKDFFGSEVARRYPEGLVPQGISAELIAAKWNLSREQLDTFAAESHRRA
HHHCCCCCHHHCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
AQAWSEGRFARDVVPIKAPDVNGDLVEVSTDESVRPSTTVEVLAGLKPAFRNELWQQRFP
HHHHHCCCHHHCEECEECCCCCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHCC
GLDWKVTAGNSSPINDGASAVLITSSETAHRLGLTPRARVHSVAVVGDDPLYMLTGIIPA
CCCEEEECCCCCCCCCCCCEEEEECCCCHHHHCCCCCHHHEEEEEECCCCCEEHHHHHHH
TAKVLDRAGLSLADIDAFEVNEAFASVVLAWQAETGADLSKVNVNGGATAIGHPLGASGG
HHHHHHHCCCCEECCCHHHHHHHHHHHEEEEECCCCCCCEEEECCCCCCCCCCCCCCCCC
RLMTTLVSVLEQGGGRYGLQTMCEAGGLANATILERL
HHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: Acyl group transfer; Claisen condensation [C]

Inhibitor: Tris (hydroxy methyl) aminomethane [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]