BacMap

Definition	Mycobacterium abscessus ATCC 19977 chromosome chromosome 1, complete sequence.
Accession	NC_010397
Length	5,067,172

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The map label for this gene is echA17 [H]

Identifier: 169630463

GI number: 169630463

Start: 3431982

End: 3432776

Strand: Reverse

Name: echA17 [H]

Synonym: MAB_3382c

Alternate gene names: 169630463

Gene position: 3432776-3431982 (Counterclockwise)

Preceding gene: 169630464

Following gene: 169630462

Centisome position: 67.75

GC content: 67.04

Gene sequence:

>795_bases
ATGATCGAGCCCGAATTCGTGTCGGTGCGCACCGCGCCCGAGCATCCCGGCATCGGCACCATCGCATTGTCGCGGCCGCC
CACCAACACGTTCACCCGGCAGATGTATCGCGAACTGGCCCAGGCCGCCGCGGAGGTGAACGCACGCGCAGACATCAGGG
TTGTCATCGTCTATGGGGGACACGAGATTTTCTGCGCCGGTGACGATTTGGCGGAACTTTCGGAGCTATCCGCCGATGAG
ATGGCGCGCAGTGCCGGTGATCGGCAGGCTGCGCTTACCGCGGTGGCGGAGCTGGCCAAACCCACGATCGCCGCGATCAC
GGGGTATGCGCTGGGCAGCGGACTGGAGCTGGCCCTGACGGCCGACTGGCGGATCGCAGGGGACAACGTCAAGGTGGGTT
CGCCGGAGATCCTGGTCGGCATGATTCCGGGTGGCGGTGGGACTGTCCGACTGGCGCGTGTGGTGGGTCTGAGCCGGGCC
AAGGAGATGGTCTTCAGCGGCCGCTTCGTGGGAGCTGACGAAGCACGGGGCGCTGGACTCATCGATCAGGTGGTGGCGCC
CGACGATGTGTATACAGAGGCCGCGAGCTGGGCCCGGCGCTTTGTCGATGGGCCGCCGCTGGCGCTCGCTGCCGCCAAGG
AGGCCATCGACCATGGATTCGACGGTCCGCTGGCCGCTGGGCTGGACAAGGAACGTGAGCTGTTTTGCCAGGTGTTCGCG
ACACAGGACCGCGCGGACGGCGTCACCGCTCAGCTCGAGATCGGCCCGGGGACGGCCCGGTTCAGGGGAGCCTAA

Upstream 100 bases:

>100_bases
AGGATGGCCGGTGGTTCGTCCAATTCGACGACGTCGATCGATACGAAGCAGCCCGGCAGGGTGCGGTCGAAATCCCCGAG
GTGACCGGGCCGGAGCTGTC

Downstream 100 bases:

>100_bases
CCAGGAGTTAGGCTGACGGCCATGACGGAGATTACGGGGCTGGACGGCCAGCCGACCACAAAGGCCACCGCCGAGCAGGT
CGCCGCCGCTTTCGAGGACA

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MIEPEFVSVRTAPEHPGIGTIALSRPPTNTFTRQMYRELAQAAAEVNARADIRVVIVYGGHEIFCAGDDLAELSELSADE
MARSAGDRQAALTAVAELAKPTIAAITGYALGSGLELALTADWRIAGDNVKVGSPEILVGMIPGGGGTVRLARVVGLSRA
KEMVFSGRFVGADEARGAGLIDQVVAPDDVYTEAASWARRFVDGPPLALAAAKEAIDHGFDGPLAAGLDKERELFCQVFA
TQDRADGVTAQLEIGPGTARFRGA

Sequences:

>Translated_264_residues
MIEPEFVSVRTAPEHPGIGTIALSRPPTNTFTRQMYRELAQAAAEVNARADIRVVIVYGGHEIFCAGDDLAELSELSADE
MARSAGDRQAALTAVAELAKPTIAAITGYALGSGLELALTADWRIAGDNVKVGSPEILVGMIPGGGGTVRLARVVGLSRA
KEMVFSGRFVGADEARGAGLIDQVVAPDDVYTEAASWARRFVDGPPLALAAAKEAIDHGFDGPLAAGLDKERELFCQVFA
TQDRADGVTAQLEIGPGTARFRGA
>Mature_264_residues
MIEPEFVSVRTAPEHPGIGTIALSRPPTNTFTRQMYRELAQAAAEVNARADIRVVIVYGGHEIFCAGDDLAELSELSADE
MARSAGDRQAALTAVAELAKPTIAAITGYALGSGLELALTADWRIAGDNVKVGSPEILVGMIPGGGGTVRLARVVGLSRA
KEMVFSGRFVGADEARGAGLIDQVVAPDDVYTEAASWARRFVDGPPLALAAAKEAIDHGFDGPLAAGLDKERELFCQVFA
TQDRADGVTAQLEIGPGTARFRGA

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=250, Percent_Identity=34.4, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=37.109375, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI20127408, Length=163, Percent_Identity=35.5828220858896, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI68989263, Length=191, Percent_Identity=33.5078534031414, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI213417737, Length=239, Percent_Identity=29.7071129707113, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI157694516, Length=239, Percent_Identity=29.7071129707113, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI70995211, Length=265, Percent_Identity=25.6603773584906, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI157694520, Length=211, Percent_Identity=29.3838862559242, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI150378541, Length=240, Percent_Identity=27.5, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI221136756, Length=230, Percent_Identity=24.3478260869565, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI221136753, Length=230, Percent_Identity=24.3478260869565, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI221307494, Length=230, Percent_Identity=24.3478260869565, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI261878539, Length=96, Percent_Identity=44.7916666666667, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI31542718, Length=239, Percent_Identity=26.7782426778243, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI45643119, Length=207, Percent_Identity=26.0869565217391, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI260275230, Length=207, Percent_Identity=26.0869565217391, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI260274832, Length=207, Percent_Identity=26.0869565217391, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI116235487, Length=230, Percent_Identity=24.3478260869565, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=39.3574297188755, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI221142681, Length=198, Percent_Identity=36.3636363636364, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1788597, Length=221, Percent_Identity=30.316742081448, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1790281, Length=192, Percent_Identity=29.6875, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1788682, Length=180, Percent_Identity=34.4444444444444, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1787660, Length=241, Percent_Identity=32.3651452282158, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17554946, Length=249, Percent_Identity=36.5461847389558, Blast_Score=160, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI25145438, Length=235, Percent_Identity=34.8936170212766, Blast_Score=143, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17540714, Length=231, Percent_Identity=32.034632034632, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17560910, Length=215, Percent_Identity=31.1627906976744, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17536985, Length=230, Percent_Identity=30, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=225, Percent_Identity=26.6666666666667, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17558304, Length=171, Percent_Identity=28.0701754385965, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI25144276, Length=202, Percent_Identity=28.2178217821782, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17508951, Length=202, Percent_Identity=28.2178217821782, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17508953, Length=202, Percent_Identity=28.2178217821782, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=197, Percent_Identity=29.4416243654822, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=243, Percent_Identity=23.8683127572016, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI20129971, Length=258, Percent_Identity=37.2093023255814, Blast_Score=161, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24653477, Length=258, Percent_Identity=37.2093023255814, Blast_Score=161, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24653139, Length=240, Percent_Identity=35.4166666666667, Blast_Score=138, Evalue=4e-33,
Organism=Drosophila melanogaster, GI21357171, Length=175, Percent_Identity=32, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI19920382, Length=262, Percent_Identity=25.9541984732824, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24650670, Length=204, Percent_Identity=31.8627450980392, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921000, Length=136, Percent_Identity=38.2352941176471, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583077, Length=136, Percent_Identity=38.2352941176471, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24583079, Length=136, Percent_Identity=38.2352941176471, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19921018, Length=196, Percent_Identity=24.4897959183673, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24654903, Length=226, Percent_Identity=22.5663716814159, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24583165, Length=202, Percent_Identity=23.2673267326733, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922422, Length=246, Percent_Identity=22.7642276422764, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27647; Mature: 27647

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEPEFVSVRTAPEHPGIGTIALSRPPTNTFTRQMYRELAQAAAEVNARADIRVVIVYGG
CCCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC
HEIFCAGDDLAELSELSADEMARSAGDRQAALTAVAELAKPTIAAITGYALGSGLELALT
CEEEECCCCHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEE
ADWRIAGDNVKVGSPEILVGMIPGGGGTVRLARVVGLSRAKEMVFSGRFVGADEARGAGL
ECEEEECCCEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCC
IDQVVAPDDVYTEAASWARRFVDGPPLALAAAKEAIDHGFDGPLAAGLDKERELFCQVFA
HHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHH
TQDRADGVTAQLEIGPGTARFRGA
CCCCCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MIEPEFVSVRTAPEHPGIGTIALSRPPTNTFTRQMYRELAQAAAEVNARADIRVVIVYGG
CCCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC
HEIFCAGDDLAELSELSADEMARSAGDRQAALTAVAELAKPTIAAITGYALGSGLELALT
CEEEECCCCHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEE
ADWRIAGDNVKVGSPEILVGMIPGGGGTVRLARVVGLSRAKEMVFSGRFVGADEARGAGL
ECEEEECCCEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCC
IDQVVAPDDVYTEAASWARRFVDGPPLALAAAKEAIDHGFDGPLAAGLDKERELFCQVFA
HHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHH
TQDRADGVTAQLEIGPGTARFRGA
CCCCCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]