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The map label for this gene is leuB [H]

Identifier: 169630384

GI number: 169630384

Start: 3341659

End: 3342672

Strand: Reverse

Name: leuB [H]

Synonym: MAB_3303c

Alternate gene names: 169630384

Gene position: 3342672-3341659 (Counterclockwise)

Preceding gene: 169630385

Following gene: 169630381

Centisome position: 65.97

GC content: 65.38

Gene sequence:

>1014_bases
ATGACCAAACTCGCGATCATCGCCGGTGATGGCATCGGTCCTGAGGTCATCGGCGAGGCCGTGAAGGTGCTGGATGCGGT
ACTGCCGGGCGTCGACAAGACCGAATACGACTTGGGTGCTCGCCGCTACCTGGCGACCGGCGAGCTGATGCCCGACTCGG
TGCTCGATGAGCTACGCGGACACGACGCCATTCTGCTTGGCGCCATTGGCGATCCGGCGGTCCCCAGCGGTGTGTTGGAG
CGGGGGCTGCTGTTGAACACCCGCTTCGCGCTGGACCACCACATCAATCTGCGACCGGCCAAGCTGTACCCCGGTGTCAC
CGGCCCGCTGGCGGGGAACCCCGCCATCGACTTGGTCGTGGTGCGCGAGGGCACTGAGGGGCCGTACACCGGCAACGGCG
GTGCGATCCGTGTCGATACCCCGCATGAGGTGGCCACCGAGGTCAGCCTCAACACCCGATTCGGGGTGGAGCGCGTGGTG
CGTAACGCATTCGAGCGTGCCGAGGCCCGTAGCAAGAACCTGACGCTGGTGCACAAGAACAACGTGCTCAAGTACGCGGG
CACGTTGTGGACGCGCACGGTCAACGAAGTCGCCGCGGAGTACCCCGATGTGCAGGTTTCCTATCAGCACATCGATGCCG
CGACCATCCACATGGTGACCGATCCGGGACGGTTCGACGTCATCGTCACCGACAACCTGTTCGGCGATATCATCACCGAC
CTGACGGCCGCCGTCACCGGTGGAATCGGTTTGGCCGCAAGCGGAAACATCGACGGCTCGGGTAAGAACCCGTCGATGTT
CGAGCCCGTCCACGGCAGCGCACCCGATATCGCGGGCCAGGGGATCGCCGATCCCACCGCCGCGATCGTGTCGGTCTCGC
TGCTGCTGGATCACCTGGGCGAGACCGAGGCCGCGGCCCGTGTCGAAAGGGCTGTCGAGCAGCATCTGGCCACCCGCGGT
GAGGAGAAGCTGTCCACCTCGGCGACTGGCGATCGGATCGCCAGCTTCCTGTAG

Upstream 100 bases:

>100_bases
CCACCATCATCCTGCGCATTGATCGCACTGCTCCGGATGCCGTGCTGGACGAGATCCGTGCCGCCGTCGGCGCGACGACC
CTTGAGCTGGTGGATCTTTC

Downstream 100 bases:

>100_bases
TCCTGATCGAATGCGAGCCTCGCGCGGGGAGATCCGCGCCGGGCTCGCATTCGGCATTAGGGGGCCGGCCGCTCCAACTT
CACCGGCACCAAACCGACTG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MTKLAIIAGDGIGPEVIGEAVKVLDAVLPGVDKTEYDLGARRYLATGELMPDSVLDELRGHDAILLGAIGDPAVPSGVLE
RGLLLNTRFALDHHINLRPAKLYPGVTGPLAGNPAIDLVVVREGTEGPYTGNGGAIRVDTPHEVATEVSLNTRFGVERVV
RNAFERAEARSKNLTLVHKNNVLKYAGTLWTRTVNEVAAEYPDVQVSYQHIDAATIHMVTDPGRFDVIVTDNLFGDIITD
LTAAVTGGIGLAASGNIDGSGKNPSMFEPVHGSAPDIAGQGIADPTAAIVSVSLLLDHLGETEAAARVERAVEQHLATRG
EEKLSTSATGDRIASFL

Sequences:

>Translated_337_residues
MTKLAIIAGDGIGPEVIGEAVKVLDAVLPGVDKTEYDLGARRYLATGELMPDSVLDELRGHDAILLGAIGDPAVPSGVLE
RGLLLNTRFALDHHINLRPAKLYPGVTGPLAGNPAIDLVVVREGTEGPYTGNGGAIRVDTPHEVATEVSLNTRFGVERVV
RNAFERAEARSKNLTLVHKNNVLKYAGTLWTRTVNEVAAEYPDVQVSYQHIDAATIHMVTDPGRFDVIVTDNLFGDIITD
LTAAVTGGIGLAASGNIDGSGKNPSMFEPVHGSAPDIAGQGIADPTAAIVSVSLLLDHLGETEAAARVERAVEQHLATRG
EEKLSTSATGDRIASFL
>Mature_336_residues
TKLAIIAGDGIGPEVIGEAVKVLDAVLPGVDKTEYDLGARRYLATGELMPDSVLDELRGHDAILLGAIGDPAVPSGVLER
GLLLNTRFALDHHINLRPAKLYPGVTGPLAGNPAIDLVVVREGTEGPYTGNGGAIRVDTPHEVATEVSLNTRFGVERVVR
NAFERAEARSKNLTLVHKNNVLKYAGTLWTRTVNEVAAEYPDVQVSYQHIDAATIHMVTDPGRFDVIVTDNLFGDIITDL
TAAVTGGIGLAASGNIDGSGKNPSMFEPVHGSAPDIAGQGIADPTAAIVSVSLLLDHLGETEAAARVERAVEQHLATRGE
EKLSTSATGDRIASFL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=310, Percent_Identity=34.5161290322581, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI4758582, Length=329, Percent_Identity=29.7872340425532, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI28178838, Length=311, Percent_Identity=29.903536977492, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI28178821, Length=299, Percent_Identity=29.4314381270903, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI28178816, Length=299, Percent_Identity=29.4314381270903, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI28178819, Length=151, Percent_Identity=33.7748344370861, Blast_Score=85, Evalue=9e-17,
Organism=Escherichia coli, GI1788101, Length=308, Percent_Identity=43.8311688311688, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI87081683, Length=356, Percent_Identity=38.7640449438202, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1787381, Length=359, Percent_Identity=29.2479108635098, Blast_Score=128, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI71986051, Length=320, Percent_Identity=33.4375, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17550882, Length=305, Percent_Identity=32.1311475409836, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25144293, Length=301, Percent_Identity=31.2292358803987, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17505779, Length=337, Percent_Identity=29.080118694362, Blast_Score=118, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324709, Length=332, Percent_Identity=34.3373493975904, Blast_Score=159, Evalue=7e-40,
Organism=Saccharomyces cerevisiae, GI6322097, Length=337, Percent_Identity=33.5311572700297, Blast_Score=139, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6324291, Length=319, Percent_Identity=32.9153605015674, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6319830, Length=334, Percent_Identity=33.2335329341317, Blast_Score=132, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24643268, Length=310, Percent_Identity=33.2258064516129, Blast_Score=174, Evalue=7e-44,
Organism=Drosophila melanogaster, GI24643270, Length=310, Percent_Identity=33.2258064516129, Blast_Score=174, Evalue=9e-44,
Organism=Drosophila melanogaster, GI24661184, Length=321, Percent_Identity=33.9563862928349, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI161078637, Length=339, Percent_Identity=27.4336283185841, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI161078635, Length=313, Percent_Identity=28.1150159744409, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI161078633, Length=339, Percent_Identity=27.4336283185841, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24650122, Length=339, Percent_Identity=27.4336283185841, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI161078639, Length=337, Percent_Identity=27.5964391691395, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI281362242, Length=318, Percent_Identity=27.3584905660377, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24648872, Length=318, Percent_Identity=27.3584905660377, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI20130355, Length=315, Percent_Identity=22.5396825396825, Blast_Score=78, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 35484; Mature: 35352

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLAIIAGDGIGPEVIGEAVKVLDAVLPGVDKTEYDLGARRYLATGELMPDSVLDELRG
CCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEECCCCCCHHHHHHHCC
HDAILLGAIGDPAVPSGVLERGLLLNTRFALDHHINLRPAKLYPGVTGPLAGNPAIDLVV
CCEEEEECCCCCCCCHHHHHCCEEEEEEEEEECCCCCCCHHCCCCCCCCCCCCCCEEEEE
VREGTEGPYTGNGGAIRVDTPHEVATEVSLNTRFGVERVVRNAFERAEARSKNLTLVHKN
EEECCCCCCCCCCCEEEECCCHHHHHEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEECC
NVLKYAGTLWTRTVNEVAAEYPDVQVSYQHIDAATIHMVTDPGRFDVIVTDNLFGDIITD
CEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEEECCCEEEEEEECCHHHHHHHH
LTAAVTGGIGLAASGNIDGSGKNPSMFEPVHGSAPDIAGQGIADPTAAIVSVSLLLDHLG
HHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
ETEAAARVERAVEQHLATRGEEKLSTSATGDRIASFL
CHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
TKLAIIAGDGIGPEVIGEAVKVLDAVLPGVDKTEYDLGARRYLATGELMPDSVLDELRG
CEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEECCCCCCHHHHHHHCC
HDAILLGAIGDPAVPSGVLERGLLLNTRFALDHHINLRPAKLYPGVTGPLAGNPAIDLVV
CCEEEEECCCCCCCCHHHHHCCEEEEEEEEEECCCCCCCHHCCCCCCCCCCCCCCEEEEE
VREGTEGPYTGNGGAIRVDTPHEVATEVSLNTRFGVERVVRNAFERAEARSKNLTLVHKN
EEECCCCCCCCCCCEEEECCCHHHHHEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEECC
NVLKYAGTLWTRTVNEVAAEYPDVQVSYQHIDAATIHMVTDPGRFDVIVTDNLFGDIITD
CEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEEECCCEEEEEEECCHHHHHHHH
LTAAVTGGIGLAASGNIDGSGKNPSMFEPVHGSAPDIAGQGIADPTAAIVSVSLLLDHLG
HHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
ETEAAARVERAVEQHLATRGEEKLSTSATGDRIASFL
CHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA