Click here to switch to the map view.

The map label for this gene is bglA [H]

Identifier: 169630274

GI number: 169630274

Start: 3236768

End: 3238153

Strand: Reverse

Name: bglA [H]

Synonym: MAB_3192c

Alternate gene names: 169630274

Gene position: 3238153-3236768 (Counterclockwise)

Preceding gene: 169630275

Following gene: 169630272

Centisome position: 63.9

GC content: 62.99

Gene sequence:

>1386_bases
ATGAAGTGGATGAACGCCGTCATGGCATCGGCAGCTGTGGTCGCACTGGGGTTGACGGCCTGCAGCCCTCGGCAGGAGGC
CCCCAAGGAGGATGCCGGCTGGGATCGCGGGTTCTACTGGGGCACCGCCACCGCGGGTTACCAGGTAGAGGGCAGTGCGC
CCGACAGCAATTGGCGCCGTTACGTCGAGCGCAGCGCGGGTAAACCCGTACACCCCGACGAATCGAATCTCCTCGCGGAT
GGCCCCGTGGATCCCTATAAGCAGGCCGATGATTTCCGGCACCGGTACGCCGAAGACATCGCCAATGCGCATGCCATGGG
CGTCAACACATTTCGATTCGGCCTGGAGTGGTCCAGGGTGATGCCTGAACCGGGTAAATGGGATGAGAAGGAGCTGGCGT
ACTACGACTCCGTTGTCGCCACACTGCGTGAAAACGGCATGACACCGATGATCACGCTCATGCACTGGGTGTATCCGGGG
TGGGTTGCCGACAGCGGCGGGTTCATGAACAATCTCCCCGCTTTTGAGGAATTCGCCAAGGCGATCACCAAACGTTATGC
CGGGCAAGGGGTGCTGTGGGTCAGCATCAACGAACCGCTGGCCTTCGGGGCGATGGAGGTGCGCACGGGCGCGATCAAGC
CTGACCAGTTCAACGGGTTTCTGGACCGGGTCGCCGATGCGCACCGTGCGGTATACCGCGCCGCCCACGAGGCCGACCCC
GAGGCGAAGGTGACGACCAATGAGGCCTACATCCCACCGGATGTGTTGGCGCAGTTCGCAGGTTTCGGCGTGAAGGGTAT
CGAGGGTTCGTTCTTCGACCGGGTTGCCGGCAGCCTCGACTATCTGGGGTTCGACTACTACACCGGGACCGCGGCAGACA
ATCCGGCATCGGCGCAGAGCATGGCGGCCCGCTGGAACATCAAACTTCAGCCCGAAGACATCTACTACGTGTCACGGCAT
TACGCACAGCGCTACCCGGGACTGCCGATCTACATCGTCGAGAACGGGATGGTGACCGACAATGGCAAGCCGCGGGCCGA
CGGGGTGACGCGATCGCAGTACTTGAGCGACACCGTCTTTTGGCTGCAGCGGGCCAAGGCGGACGGAATCCCGATCGTCG
GCTACAACTACTGGTCTTTGGTCGATAACTACGAGTGGGGAAGCTACCGGCCACGTTTCGGTCTGTATACCGTTGATGCA
TTGGGTGATCCGGCGCTCAAGCGGGTGCCCACCGATGCCGTCGCAACGTACACCCAGATCACCCGGGACGCGGGGACTGT
GCCCGGATACCGGCCGGTCATTCCGGCGGCGAAGTGCTCCACGACGGTGGGACAGGACAGCTGCGCCCCGCTGGATCCGA
ACGGGCCCACGGTCGCGCTGCGTTAG

Upstream 100 bases:

>100_bases
TCCAGTGGATGTCGGACCCGTCCATCGATATGGGCGAGCACATGCGCCGGGTGTGGGAGTTGCTGCTCAGGTAAGAAACC
GTACGGTGTATGGTTTTGTC

Downstream 100 bases:

>100_bases
ACGCCCTGGGTGTAGCCGCCCGTACCATGAAGTCGCGTTCCGGGTGCTGCGGGGCCACCGCGGGGTCGCCGTCGACGACG
ATATCGGCGCGACCGGCGGT

Product: putative glycosyl hydrolase (beta-glucosidase)

Products: NA

Alternate protein names: BGA; Amygdalase; Beta-D-glucoside glucohydrolase; Cellobiase; Gentiobiase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKWMNAVMASAAVVALGLTACSPRQEAPKEDAGWDRGFYWGTATAGYQVEGSAPDSNWRRYVERSAGKPVHPDESNLLAD
GPVDPYKQADDFRHRYAEDIANAHAMGVNTFRFGLEWSRVMPEPGKWDEKELAYYDSVVATLRENGMTPMITLMHWVYPG
WVADSGGFMNNLPAFEEFAKAITKRYAGQGVLWVSINEPLAFGAMEVRTGAIKPDQFNGFLDRVADAHRAVYRAAHEADP
EAKVTTNEAYIPPDVLAQFAGFGVKGIEGSFFDRVAGSLDYLGFDYYTGTAADNPASAQSMAARWNIKLQPEDIYYVSRH
YAQRYPGLPIYIVENGMVTDNGKPRADGVTRSQYLSDTVFWLQRAKADGIPIVGYNYWSLVDNYEWGSYRPRFGLYTVDA
LGDPALKRVPTDAVATYTQITRDAGTVPGYRPVIPAAKCSTTVGQDSCAPLDPNGPTVALR

Sequences:

>Translated_461_residues
MKWMNAVMASAAVVALGLTACSPRQEAPKEDAGWDRGFYWGTATAGYQVEGSAPDSNWRRYVERSAGKPVHPDESNLLAD
GPVDPYKQADDFRHRYAEDIANAHAMGVNTFRFGLEWSRVMPEPGKWDEKELAYYDSVVATLRENGMTPMITLMHWVYPG
WVADSGGFMNNLPAFEEFAKAITKRYAGQGVLWVSINEPLAFGAMEVRTGAIKPDQFNGFLDRVADAHRAVYRAAHEADP
EAKVTTNEAYIPPDVLAQFAGFGVKGIEGSFFDRVAGSLDYLGFDYYTGTAADNPASAQSMAARWNIKLQPEDIYYVSRH
YAQRYPGLPIYIVENGMVTDNGKPRADGVTRSQYLSDTVFWLQRAKADGIPIVGYNYWSLVDNYEWGSYRPRFGLYTVDA
LGDPALKRVPTDAVATYTQITRDAGTVPGYRPVIPAAKCSTTVGQDSCAPLDPNGPTVALR
>Mature_461_residues
MKWMNAVMASAAVVALGLTACSPRQEAPKEDAGWDRGFYWGTATAGYQVEGSAPDSNWRRYVERSAGKPVHPDESNLLAD
GPVDPYKQADDFRHRYAEDIANAHAMGVNTFRFGLEWSRVMPEPGKWDEKELAYYDSVVATLRENGMTPMITLMHWVYPG
WVADSGGFMNNLPAFEEFAKAITKRYAGQGVLWVSINEPLAFGAMEVRTGAIKPDQFNGFLDRVADAHRAVYRAAHEADP
EAKVTTNEAYIPPDVLAQFAGFGVKGIEGSFFDRVAGSLDYLGFDYYTGTAADNPASAQSMAARWNIKLQPEDIYYVSRH
YAQRYPGLPIYIVENGMVTDNGKPRADGVTRSQYLSDTVFWLQRAKADGIPIVGYNYWSLVDNYEWGSYRPRFGLYTVDA
LGDPALKRVPTDAVATYTQITRDAGTVPGYRPVIPAAKCSTTVGQDSCAPLDPNGPTVALR

Specific function: BglA is intracellular and cleaves cellobiose probably through inorganic phosphate mediated hydrolysis [H]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI32481206, Length=172, Percent_Identity=29.0697674418605, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI13273313, Length=187, Percent_Identity=27.2727272727273, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI110681710, Length=189, Percent_Identity=24.8677248677249, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI2367270, Length=464, Percent_Identity=25, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI2367174, Length=466, Percent_Identity=25.7510729613734, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789070, Length=471, Percent_Identity=23.9915074309979, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17552856, Length=173, Percent_Identity=28.9017341040462, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17539390, Length=173, Percent_Identity=29.4797687861272, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356577, Length=171, Percent_Identity=28.6549707602339, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017736
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.21 [H]

Molecular weight: Translated: 50936; Mature: 50936

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWMNAVMASAAVVALGLTACSPRQEAPKEDAGWDRGFYWGTATAGYQVEGSAPDSNWRR
CCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCEEEECCCCCEEEECCCCCHHHHH
YVERSAGKPVHPDESNLLADGPVDPYKQADDFRHRYAEDIANAHAMGVNTFRFGLEWSRV
HHHHHCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHC
MPEPGKWDEKELAYYDSVVATLRENGMTPMITLMHWVYPGWVADSGGFMNNLPAFEEFAK
CCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHH
AITKRYAGQGVLWVSINEPLAFGAMEVRTGAIKPDQFNGFLDRVADAHRAVYRAAHEADP
HHHHHHCCCCEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
EAKVTTNEAYIPPDVLAQFAGFGVKGIEGSFFDRVAGSLDYLGFDYYTGTAADNPASAQS
CCEEECCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHCCHHHCCEEEEECCCCCCCCHHHH
MAARWNIKLQPEDIYYVSRHYAQRYPGLPIYIVENGMVTDNGKPRADGVTRSQYLSDTVF
HHHEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCEECCCCCCCCCCCHHHHHHHHHHH
WLQRAKADGIPIVGYNYWSLVDNYEWGSYRPRFGLYTVDALGDPALKRVPTDAVATYTQI
HHHHHCCCCCCEECCHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHCCCHHHHHHHHH
TRDAGTVPGYRPVIPAAKCSTTVGQDSCAPLDPNGPTVALR
HHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEEC
>Mature Secondary Structure
MKWMNAVMASAAVVALGLTACSPRQEAPKEDAGWDRGFYWGTATAGYQVEGSAPDSNWRR
CCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCEEEECCCCCEEEECCCCCHHHHH
YVERSAGKPVHPDESNLLADGPVDPYKQADDFRHRYAEDIANAHAMGVNTFRFGLEWSRV
HHHHHCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHC
MPEPGKWDEKELAYYDSVVATLRENGMTPMITLMHWVYPGWVADSGGFMNNLPAFEEFAK
CCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHH
AITKRYAGQGVLWVSINEPLAFGAMEVRTGAIKPDQFNGFLDRVADAHRAVYRAAHEADP
HHHHHHCCCCEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
EAKVTTNEAYIPPDVLAQFAGFGVKGIEGSFFDRVAGSLDYLGFDYYTGTAADNPASAQS
CCEEECCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHCCHHHCCEEEEECCCCCCCCHHHH
MAARWNIKLQPEDIYYVSRHYAQRYPGLPIYIVENGMVTDNGKPRADGVTRSQYLSDTVF
HHHEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCEECCCCCCCCCCCHHHHHHHHHHH
WLQRAKADGIPIVGYNYWSLVDNYEWGSYRPRFGLYTVDALGDPALKRVPTDAVATYTQI
HHHHHCCCCCCEECCHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHCCCHHHHHHHHH
TRDAGTVPGYRPVIPAAKCSTTVGQDSCAPLDPNGPTVALR
HHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2123813; 9466926 [H]