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The map label for this gene is rlmN [H]

Identifier: 169630257

GI number: 169630257

Start: 3221487

End: 3222608

Strand: Reverse

Name: rlmN [H]

Synonym: MAB_3175c

Alternate gene names: 169630257

Gene position: 3222608-3221487 (Counterclockwise)

Preceding gene: 169630258

Following gene: 169630255

Centisome position: 63.6

GC content: 66.13

Gene sequence:

>1122_bases
ATGCCTGCCGATAACAAGCCCTTGCCGCTGGTCTTCGACGCACCGCGTCGTGCCATGCCGCCCCGTCATCTGGCCGATAT
GAGCCTTGAGCAGAGCCGTGACGTGGTCACCGAATTGGGGCTTCCCGCATTTCGTGCCAAGCAGATCGCCAACCAGTATT
ACGGAAGGCTGGTGGGTGACCCGGCCACGATGACGGACCTGCCGGCCGGTGCCCGCGGAGGGGTGGCCGAGGCGCTGTTT
CCCCGGTTGCTCGAGCCACTGCGCCAGATTGCCTGTGATGCGGGCGATACCCGAAAAACCTTGTGGCGCTTGCATGACGG
TAGCACCGTCGAATCGGTGCTCATGCGTTATCCGGACCGCAACACGTTGTGCATCTCCTCTCAGGCGGGATGCGGTATGG
CCTGTCCGTTCTGCGCCACCGGCCAGGGCGGACTGACACGCAATCTGTCGGCCGCCGAGATTTTGGAACAGGTCCGCGAT
GCCGCGGCGTCCTTGCGTGACGGCCAGCTGCCGGGTGGGCCGGGCCGCTTGTCCAACATCGTCTTCATGGGCATGGGGGA
GCCCCTGGCCAACTACAACCGCGTGCTGACGGTGCTGCGCAAGATCACCGCGCCGCCGCCGGAGGGGTTCGGCATATCCC
AGCGCGGTGTCACCGTCTCGACGGTGGGACTTGCGCCGGCCATCCGGCGACTTGCCGACGAGGGGCTGGGGGTCACCCTC
GCGGTGTCGCTGCACTGTCCGGACGACGAGCTACGCGACACCCTGGTGCCGGTCAATACCCGGTGGGCGATCAGCGAGGT
CCTTGATGCCGCGCGCTACTACGCCGATGTCACGGGCCGGCGGGTATCGATCGAGTACGCCCTGATTCGTGATGTGAATG
ATCAGCCGTGGCGTGCCGACATGCTGGGCAAGAAGCTGCGAAAAGCCCTGGGGCAGATGGTCCATGTCAACCTGATTCCG
CTGAACCCGACGCCGGGCAGTCAGTGGGATGCCAGCCCCAAGCCGGTGGAACGTGAATTTGTGCGCCGCGTCCGCGAGCA
GGGTGTCTCGTGCACGGTGCGCGATACCCGCGGCCGCGAGATCGCGGCCGCGTGCGGCCAGTTGGCTGCCTCCGAGCGCT
AG

Upstream 100 bases:

>100_bases
TCGAGGTGTTGCATATTTTCGGCCCACAGGGTGAACGGATGCACGTGCGGGCACGTTCGGGTTCGGGGAACTAGCGAAAG
CCTGGGATACTGAATCGGTT

Downstream 100 bases:

>100_bases
AGCTAGCGAATGCGGCCGGAACGACGGCCGAAGTAGTCGTTGGCGGTGAACGCCAGCACGATGGCGGCAAAACCGACCAG
GAAGATGTCCTCGACCTTGC

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MPADNKPLPLVFDAPRRAMPPRHLADMSLEQSRDVVTELGLPAFRAKQIANQYYGRLVGDPATMTDLPAGARGGVAEALF
PRLLEPLRQIACDAGDTRKTLWRLHDGSTVESVLMRYPDRNTLCISSQAGCGMACPFCATGQGGLTRNLSAAEILEQVRD
AAASLRDGQLPGGPGRLSNIVFMGMGEPLANYNRVLTVLRKITAPPPEGFGISQRGVTVSTVGLAPAIRRLADEGLGVTL
AVSLHCPDDELRDTLVPVNTRWAISEVLDAARYYADVTGRRVSIEYALIRDVNDQPWRADMLGKKLRKALGQMVHVNLIP
LNPTPGSQWDASPKPVEREFVRRVREQGVSCTVRDTRGREIAAACGQLAASER

Sequences:

>Translated_373_residues
MPADNKPLPLVFDAPRRAMPPRHLADMSLEQSRDVVTELGLPAFRAKQIANQYYGRLVGDPATMTDLPAGARGGVAEALF
PRLLEPLRQIACDAGDTRKTLWRLHDGSTVESVLMRYPDRNTLCISSQAGCGMACPFCATGQGGLTRNLSAAEILEQVRD
AAASLRDGQLPGGPGRLSNIVFMGMGEPLANYNRVLTVLRKITAPPPEGFGISQRGVTVSTVGLAPAIRRLADEGLGVTL
AVSLHCPDDELRDTLVPVNTRWAISEVLDAARYYADVTGRRVSIEYALIRDVNDQPWRADMLGKKLRKALGQMVHVNLIP
LNPTPGSQWDASPKPVEREFVRRVREQGVSCTVRDTRGREIAAACGQLAASER
>Mature_372_residues
PADNKPLPLVFDAPRRAMPPRHLADMSLEQSRDVVTELGLPAFRAKQIANQYYGRLVGDPATMTDLPAGARGGVAEALFP
RLLEPLRQIACDAGDTRKTLWRLHDGSTVESVLMRYPDRNTLCISSQAGCGMACPFCATGQGGLTRNLSAAEILEQVRDA
AASLRDGQLPGGPGRLSNIVFMGMGEPLANYNRVLTVLRKITAPPPEGFGISQRGVTVSTVGLAPAIRRLADEGLGVTLA
VSLHCPDDELRDTLVPVNTRWAISEVLDAARYYADVTGRRVSIEYALIRDVNDQPWRADMLGKKLRKALGQMVHVNLIPL
NPTPGSQWDASPKPVEREFVRRVREQGVSCTVRDTRGREIAAACGQLAASER

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=355, Percent_Identity=37.7464788732394, Blast_Score=212, Evalue=3e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 40523; Mature: 40392

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPADNKPLPLVFDAPRRAMPPRHLADMSLEQSRDVVTELGLPAFRAKQIANQYYGRLVGD
CCCCCCCCCEEECCCCCCCCCHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
PATMTDLPAGARGGVAEALFPRLLEPLRQIACDAGDTRKTLWRLHDGSTVESVLMRYPDR
CCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHEEECCCCHHHHHHHHCCCC
NTLCISSQAGCGMACPFCATGQGGLTRNLSAAEILEQVRDAAASLRDGQLPGGPGRLSNI
CEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
VFMGMGEPLANYNRVLTVLRKITAPPPEGFGISQRGVTVSTVGLAPAIRRLADEGLGVTL
EEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCEEE
AVSLHCPDDELRDTLVPVNTRWAISEVLDAARYYADVTGRRVSIEYALIRDVNDQPWRAD
EEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCHHH
MLGKKLRKALGQMVHVNLIPLNPTPGSQWDASPKPVEREFVRRVREQGVSCTVRDTRGRE
HHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCH
IAAACGQLAASER
HHHHHHHHHCCCC
>Mature Secondary Structure 
PADNKPLPLVFDAPRRAMPPRHLADMSLEQSRDVVTELGLPAFRAKQIANQYYGRLVGD
CCCCCCCCEEECCCCCCCCCHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
PATMTDLPAGARGGVAEALFPRLLEPLRQIACDAGDTRKTLWRLHDGSTVESVLMRYPDR
CCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHEEECCCCHHHHHHHHCCCC
NTLCISSQAGCGMACPFCATGQGGLTRNLSAAEILEQVRDAAASLRDGQLPGGPGRLSNI
CEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
VFMGMGEPLANYNRVLTVLRKITAPPPEGFGISQRGVTVSTVGLAPAIRRLADEGLGVTL
EEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCEEE
AVSLHCPDDELRDTLVPVNTRWAISEVLDAARYYADVTGRRVSIEYALIRDVNDQPWRAD
EEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCHHH
MLGKKLRKALGQMVHVNLIPLNPTPGSQWDASPKPVEREFVRRVREQGVSCTVRDTRGRE
HHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCH
IAAACGQLAASER
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA