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The map label for this gene is acr1 [H]

Identifier: 169630026

GI number: 169630026

Start: 2999320

End: 3000219

Strand: Reverse

Name: acr1 [H]

Synonym: MAB_2943c

Alternate gene names: 169630026

Gene position: 3000219-2999320 (Counterclockwise)

Preceding gene: 169630027

Following gene: 169630020

Centisome position: 59.21

GC content: 66.78

Gene sequence:

>900_bases
ATGCGGGCCGTCAGGCACATGATCAACGTCTTCACCAAGCCCATTGGAGTCACCTACCCCTGGACGCCGCCGCTCGCCGA
GCGCGTGCATGGCCGTACCGTGTTGGTCACCGGCGCGTCAAGCGGGATCGGCCGGAGGCTGGCCGAACGCGTAGCCGAGG
CGGGAGGCATCGTCATCGTCACCGGGCGGCGTGCCGATGAGCTCGGCGAGGTGGTGCGCGGTATCACCGCACGTGGTGGC
GCTGCCCACGCTCTGCCCGGTGATCTGTCCACGGCCGCGGGGGCGGACGCGGTGGCCGATGCGGTGCTCGAAGGCTTTGG
GGCGCCGGACATTCTGGTGCTGAATGCGGGCCGATCGATCATGCGTTCGGTGGCTGATTCCGAGTATCGGCTTCATGACT
ATGAGCGGACGGTCGCGATCAACTACCTCGGCGGCGTCGGACTGGTGCTGCGCCTGCTGCCCGGCATGCGTGCCCGCGGT
TCGGGGCATATTGTGCACAGCTCGTCGATCGGGGTGCTGGGCAACCTGCCCGAATTCTCCGCCTATGTCGGATCGAAGGC
GGCCATGGATGCGGTGCTGCGCATCGCGGGTATCGAGAGCCGGGCCGACGGCGTGCGCGTCACCAATGTCCATCTGCCAT
TGGTCTCGACCGACATGATCGCCCCGACGGACTGGAGCGAGTATGCCTCACTGACGCTGGATGAGGGTGTCGACATGGTC
GTCGGGGCGATTCGGCGGCAGAGCCCCGAAGTGAACAATCCGTTGGGCATCCTGTACCGCGACGCCTATCGCGCGGTGCC
GGGGATTGTCAGTAGGGTGCAGAACGACTACTACCGCTGGTATTCCTCGCGCGGCCAGAAGGCGGGAGCGGCGCCGGCCT
CGTCTCCCGGCATCGGCTAA

Upstream 100 bases:

>100_bases
CGCTCATCCGCAAGTCTGCGCGCCATCTTGACAACGTGCGATGTCAACATGATGATGGCTCGTGAAAAAGCCGACAGCGC
AGGGAAAATGGAGTGATCGG

Downstream 100 bases:

>100_bases
CCCGAAGCGGAACCGATGAATTCCAGGCCCGCGTTGATGGCGGGGCGGTGCCGCAAGATTCGGTAGTGCGCGAGCCGGCC
GAGTCCCTTCGTGGCCATGA

Product: putative oxidoreductase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MRAVRHMINVFTKPIGVTYPWTPPLAERVHGRTVLVTGASSGIGRRLAERVAEAGGIVIVTGRRADELGEVVRGITARGG
AAHALPGDLSTAAGADAVADAVLEGFGAPDILVLNAGRSIMRSVADSEYRLHDYERTVAINYLGGVGLVLRLLPGMRARG
SGHIVHSSSIGVLGNLPEFSAYVGSKAAMDAVLRIAGIESRADGVRVTNVHLPLVSTDMIAPTDWSEYASLTLDEGVDMV
VGAIRRQSPEVNNPLGILYRDAYRAVPGIVSRVQNDYYRWYSSRGQKAGAAPASSPGIG

Sequences:

>Translated_299_residues
MRAVRHMINVFTKPIGVTYPWTPPLAERVHGRTVLVTGASSGIGRRLAERVAEAGGIVIVTGRRADELGEVVRGITARGG
AAHALPGDLSTAAGADAVADAVLEGFGAPDILVLNAGRSIMRSVADSEYRLHDYERTVAINYLGGVGLVLRLLPGMRARG
SGHIVHSSSIGVLGNLPEFSAYVGSKAAMDAVLRIAGIESRADGVRVTNVHLPLVSTDMIAPTDWSEYASLTLDEGVDMV
VGAIRRQSPEVNNPLGILYRDAYRAVPGIVSRVQNDYYRWYSSRGQKAGAAPASSPGIG
>Mature_299_residues
MRAVRHMINVFTKPIGVTYPWTPPLAERVHGRTVLVTGASSGIGRRLAERVAEAGGIVIVTGRRADELGEVVRGITARGG
AAHALPGDLSTAAGADAVADAVLEGFGAPDILVLNAGRSIMRSVADSEYRLHDYERTVAINYLGGVGLVLRLLPGMRARG
SGHIVHSSSIGVLGNLPEFSAYVGSKAAMDAVLRIAGIESRADGVRVTNVHLPLVSTDMIAPTDWSEYASLTLDEGVDMV
VGAIRRQSPEVNNPLGILYRDAYRAVPGIVSRVQNDYYRWYSSRGQKAGAAPASSPGIG

Specific function: Catalyzes the NADPH-dependent reduction of long chain acyl-CoA to the corresponding aldehyde [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI7706318, Length=160, Percent_Identity=37.5, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI20149619, Length=192, Percent_Identity=32.8125, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI32455239, Length=165, Percent_Identity=32.1212121212121, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI5031765, Length=165, Percent_Identity=32.1212121212121, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI40807363, Length=216, Percent_Identity=26.8518518518519, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1787905, Length=143, Percent_Identity=31.4685314685315, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1786701, Length=198, Percent_Identity=28.7878787878788, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17560150, Length=213, Percent_Identity=32.3943661971831, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17562990, Length=208, Percent_Identity=33.1730769230769, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17562904, Length=198, Percent_Identity=32.3232323232323, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17565030, Length=221, Percent_Identity=32.1266968325792, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17562906, Length=191, Percent_Identity=35.6020942408377, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17559104, Length=196, Percent_Identity=33.1632653061224, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17538480, Length=204, Percent_Identity=32.843137254902, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI193204405, Length=191, Percent_Identity=35.0785340314136, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17538486, Length=196, Percent_Identity=32.1428571428571, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17563726, Length=207, Percent_Identity=28.0193236714976, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI72000259, Length=215, Percent_Identity=28.3720930232558, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17551412, Length=203, Percent_Identity=30.5418719211823, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17562908, Length=194, Percent_Identity=34.5360824742268, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17562910, Length=199, Percent_Identity=31.6582914572864, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17531453, Length=193, Percent_Identity=35.2331606217617, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560332, Length=197, Percent_Identity=31.4720812182741, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17508895, Length=215, Percent_Identity=28.3720930232558, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17564282, Length=235, Percent_Identity=26.8085106382979, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17560220, Length=192, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17544670, Length=206, Percent_Identity=30.5825242718447, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=199, Percent_Identity=31.6582914572864, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=244, Percent_Identity=27.8688524590164, Blast_Score=66, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21358495, Length=186, Percent_Identity=34.4086021505376, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21357041, Length=189, Percent_Identity=31.7460317460317, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI28571526, Length=189, Percent_Identity=32.8042328042328, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24644339, Length=192, Percent_Identity=30.7291666666667, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI116007236, Length=215, Percent_Identity=30.2325581395349, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI116007238, Length=194, Percent_Identity=29.8969072164948, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24643142, Length=202, Percent_Identity=30.1980198019802, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24641232, Length=208, Percent_Identity=28.8461538461538, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI23397609, Length=188, Percent_Identity=29.7872340425532, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 31546; Mature: 31546

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAVRHMINVFTKPIGVTYPWTPPLAERVHGRTVLVTGASSGIGRRLAERVAEAGGIVIV
CHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCEEEE
TGRRADELGEVVRGITARGGAAHALPGDLSTAAGADAVADAVLEGFGAPDILVLNAGRSI
ECCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCHHHHHHHHHHHCCCCCEEEECCCHHH
MRSVADSEYRLHDYERTVAINYLGGVGLVLRLLPGMRARGSGHIVHSSSIGVLGNLPEFS
HHHHHCCCCEEHHHHHEEEEEHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCHHH
AYVGSKAAMDAVLRIAGIESRADGVRVTNVHLPLVSTDMIAPTDWSEYASLTLDEGVDMV
HHHCCHHHHHHHHHHHCCCCCCCCEEEEEEEECEEECCCCCCCCHHHHHHEEHHCCHHHH
VGAIRRQSPEVNNPLGILYRDAYRAVPGIVSRVQNDYYRWYSSRGQKAGAAPASSPGIG
HHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRAVRHMINVFTKPIGVTYPWTPPLAERVHGRTVLVTGASSGIGRRLAERVAEAGGIVIV
CHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCEEEE
TGRRADELGEVVRGITARGGAAHALPGDLSTAAGADAVADAVLEGFGAPDILVLNAGRSI
ECCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCHHHHHHHHHHHCCCCCEEEECCCHHH
MRSVADSEYRLHDYERTVAINYLGGVGLVLRLLPGMRARGSGHIVHSSSIGVLGNLPEFS
HHHHHCCCCEEHHHHHEEEEEHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCHHH
AYVGSKAAMDAVLRIAGIESRADGVRVTNVHLPLVSTDMIAPTDWSEYASLTLDEGVDMV
HHHCCHHHHHHHHHHHCCCCCCCCEEEEEEEECEEECCCCCCCCHHHHHHEEHHCCHHHH
VGAIRRQSPEVNNPLGILYRDAYRAVPGIVSRVQNDYYRWYSSRGQKAGAAPASSPGIG
HHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA