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The map label for this gene is phbC [H]

Identifier: 169630000

GI number: 169630000

Start: 2967654

End: 2968700

Strand: Direct

Name: phbC [H]

Synonym: MAB_2917

Alternate gene names: 169630000

Gene position: 2967654-2968700 (Clockwise)

Preceding gene: 169629999

Following gene: 169630004

Centisome position: 58.57

GC content: 62.27

Gene sequence:

>1047_bases
ATGGTCTCGATCCCGGTCTCGGCGAGGAACTTGTTCGCCATCACTTTCGGCAACGGAGTGCGCCCACCCACACCGACCAG
CCATGTTGCGCTGTTCGACGAGCCACATCGCCAATTGCGCCGTTACGGCACTGACGATCCGCGCGACTCCAGCGCTGTCC
CGGTCCTTTTGGTTCCGCCGCTGGCGGCGTCGGCGACATGCTATGACCTTGCACCGGGTCAGTCACTGGTGGCGCACCTG
CTCGAGCAGGGCCGTACGCCCTATGTCGTCGACTACGGTGAGATCGGCTGGGCAGACCGGCATCTGGGTCTGGAAGCATT
CTTCGCCGACATCATTCCGCAGGCCATCGAGCGGGTACTCATCGATTCGGGTAAAACACAGCTCGACCTGATTGGGTGGA
GCCTGGGCGGCACCCTGAGCCTCTTGACCGCCGCGGCCGACCGCGGATTGCCGATCCGCTCGATCGTCGCTATCGGCACC
CCATTGGACTACGGACAAATCCCCGGCTATCCCGAGGCACGCCGAATCACCAAACGCACCAACGGCTATGCCGTCACCAC
GGTGTTACGAGCACTCGGCGGCATTCCTGCACCGGTGGTACAGGCCGCCTACCGCGCCACGTCATGGGACCGAGAAATCA
AGCGGCCCTGGTTTCTGCTGAACAATCTGGACAACACCGAAGCCTTGGCCAAGGCCGAAGTGATCGACCGGTTTCAGGAT
GCCTTCCCTGGTTATCCAGGACGGGCCGTCTCACAGATGTGGGGCCGCTTTATCTATCACGATGAGATCGCCGCTGGCGT
GGTCAATGTGGCCGGTTACACCCTCGATCTGACGACTCTGACGTTGCCGATCCAGCTGTTCGGCAGCCACCGTGATGCCA
TCGCGCCGTGGCAGTGTGTGCATCACGGCGTGGCGATGCTCAAGTCTGCGGATGTTCGGTTCACCACGGTGGAGGCAAGC
CATCTCGGACTGGTTGCGCTGTCGGCCGGTGTCAACGAGACCTGGCCGACCATCGATGACTTCTTGACCGAGTTGGACGG
GGGCTAG

Upstream 100 bases:

>100_bases
ACGCCACAAACCGTTGTATATCCGACCGTTCGACTGAAACGCTTTGGATGGCTATCGGTAAGCCGGGCAGTGCCGGCAAG
AAGGAGTACACGAACTAACT

Downstream 100 bases:

>100_bases
TAAGTGGCTCCTACACGACTCTTCACCCGTGCAGGGCCAGGCGTCCACCCGCAGTAGTCAGCGCTCCGACCAAAAGCGAC
CGCCGCTTGGTCGACGGGCG

Product: putative hydrolase

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MVSIPVSARNLFAITFGNGVRPPTPTSHVALFDEPHRQLRRYGTDDPRDSSAVPVLLVPPLAASATCYDLAPGQSLVAHL
LEQGRTPYVVDYGEIGWADRHLGLEAFFADIIPQAIERVLIDSGKTQLDLIGWSLGGTLSLLTAAADRGLPIRSIVAIGT
PLDYGQIPGYPEARRITKRTNGYAVTTVLRALGGIPAPVVQAAYRATSWDREIKRPWFLLNNLDNTEALAKAEVIDRFQD
AFPGYPGRAVSQMWGRFIYHDEIAAGVVNVAGYTLDLTTLTLPIQLFGSHRDAIAPWQCVHHGVAMLKSADVRFTTVEAS
HLGLVALSAGVNETWPTIDDFLTELDGG

Sequences:

>Translated_348_residues
MVSIPVSARNLFAITFGNGVRPPTPTSHVALFDEPHRQLRRYGTDDPRDSSAVPVLLVPPLAASATCYDLAPGQSLVAHL
LEQGRTPYVVDYGEIGWADRHLGLEAFFADIIPQAIERVLIDSGKTQLDLIGWSLGGTLSLLTAAADRGLPIRSIVAIGT
PLDYGQIPGYPEARRITKRTNGYAVTTVLRALGGIPAPVVQAAYRATSWDREIKRPWFLLNNLDNTEALAKAEVIDRFQD
AFPGYPGRAVSQMWGRFIYHDEIAAGVVNVAGYTLDLTTLTLPIQLFGSHRDAIAPWQCVHHGVAMLKSADVRFTTVEAS
HLGLVALSAGVNETWPTIDDFLTELDGG
>Mature_348_residues
MVSIPVSARNLFAITFGNGVRPPTPTSHVALFDEPHRQLRRYGTDDPRDSSAVPVLLVPPLAASATCYDLAPGQSLVAHL
LEQGRTPYVVDYGEIGWADRHLGLEAFFADIIPQAIERVLIDSGKTQLDLIGWSLGGTLSLLTAAADRGLPIRSIVAIGT
PLDYGQIPGYPEARRITKRTNGYAVTTVLRALGGIPAPVVQAAYRATSWDREIKRPWFLLNNLDNTEALAKAEVIDRFQD
AFPGYPGRAVSQMWGRFIYHDEIAAGVVNVAGYTLDLTTLTLPIQLFGSHRDAIAPWQCVHHGVAMLKSADVRFTTVEAS
HLGLVALSAGVNETWPTIDDFLTELDGG

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010125 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: NA

Molecular weight: Translated: 37773; Mature: 37773

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSIPVSARNLFAITFGNGVRPPTPTSHVALFDEPHRQLRRYGTDDPRDSSAVPVLLVPP
CEECCCCCCEEEEEEECCCCCCCCCCCEEEECCCHHHHHHHCCCCCCCCCCCCCEEEECC
LAASATCYDLAPGQSLVAHLLEQGRTPYVVDYGEIGWADRHLGLEAFFADIIPQAIERVL
CCCCCEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHH
IDSGKTQLDLIGWSLGGTLSLLTAAADRGLPIRSIVAIGTPLDYGQIPGYPEARRITKRT
HCCCCCEEEEEEECCCCHHHHHHHHHCCCCCHHHHEEECCCCCCCCCCCCCHHHHHHHHC
NGYAVTTVLRALGGIPAPVVQAAYRATSWDREIKRPWFLLNNLDNTEALAKAEVIDRFQD
CCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHCCCEEEECCCCCHHHHHHHHHHHHHHH
AFPGYPGRAVSQMWGRFIYHDEIAAGVVNVAGYTLDLTTLTLPIQLFGSHRDAIAPWQCV
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHEEEEHHHHCCCCCCCCHHHHH
HHGVAMLKSADVRFTTVEASHLGLVALSAGVNETWPTIDDFLTELDGG
HHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MVSIPVSARNLFAITFGNGVRPPTPTSHVALFDEPHRQLRRYGTDDPRDSSAVPVLLVPP
CEECCCCCCEEEEEEECCCCCCCCCCCEEEECCCHHHHHHHCCCCCCCCCCCCCEEEECC
LAASATCYDLAPGQSLVAHLLEQGRTPYVVDYGEIGWADRHLGLEAFFADIIPQAIERVL
CCCCCEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHH
IDSGKTQLDLIGWSLGGTLSLLTAAADRGLPIRSIVAIGTPLDYGQIPGYPEARRITKRT
HCCCCCEEEEEEECCCCHHHHHHHHHCCCCCHHHHEEECCCCCCCCCCCCCHHHHHHHHC
NGYAVTTVLRALGGIPAPVVQAAYRATSWDREIKRPWFLLNNLDNTEALAKAEVIDRFQD
CCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHCCCEEEECCCCCHHHHHHHHHHHHHHH
AFPGYPGRAVSQMWGRFIYHDEIAAGVVNVAGYTLDLTTLTLPIQLFGSHRDAIAPWQCV
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHEEEEHHHHCCCCCCCCHHHHH
HHGVAMLKSADVRFTTVEASHLGLVALSAGVNETWPTIDDFLTELDGG
HHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1396692 [H]