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The map label for this gene is lip2 [H]

Identifier: 169629898

GI number: 169629898

Start: 2863382

End: 2864317

Strand: Direct

Name: lip2 [H]

Synonym: MAB_2814

Alternate gene names: 169629898

Gene position: 2863382-2864317 (Clockwise)

Preceding gene: 169629896

Following gene: 169629903

Centisome position: 56.51

GC content: 67.31

Gene sequence:

>936_bases
ATGACAGCCACCGCCGGTATCGATCCGATCATCGTCAAGATCCTGGAAGCTGTTCCGATTCAACTCGTTTCACCGGACGG
GCCGCAGGCCTCCCGGGATGCCTATCGGGCGATCGCCACGGCGCGCCCGCCGTGGATCGAGGTGGCCAACATCGAAGATC
GCACGATCCCGGGCCCCGCCGGCGAAATACCGGTTCGGATCTACTCGCCCGCCGAGGGCGGAGTACGTCCGGTTTTCGTG
CTGATCCATGGCGGCGGATGGGTCATCGGCGACCTGGACACCCACGACGCGGTGGCTCGCGCCGCGGCGGTACAGGCCGA
TGCCGTGGTGGTCTCGGTGGACTACCGGCTGGCGCCCGAGCACCCCTACCCTGCCGCGGTCGAGGATTGCTGGGCCGCCC
TGCAATGGGTGCACGAGCACGCCGCCGAGATCGGCGGCGACCCGGGCCGCCTTGCCGTGGGCGGTGATTCGGCCGGTGGA
AACCTGTCGGCAGTCATGGCGCAACTGGCCCGCGACAATGGCATTGACCTGGCTTTTCAGGTGCTGTGGTACCCAGCCAC
CACCTTTGACACGACGCTGCCGTCCATGGTGGAGAACGCCGAGGCGCCAGTGCTGGACTTCAAGACCGTGGGTGCGCTGA
GCAAGTGGTACCTCGGCGACACCAATCCGGCCACCGCGGGCCCGACGCTGGTCCCGGCCCGTGCGGAAAACTTCGAGGGG
TTGGCTCCGGCCTTTATCGGCACGGCACAGTTCGATCCGCTGCGCGATGACGGCGCCAAGTACGCCGAGCTGTTGCGTGA
CGCCGGTGTGCCCGTACAGCTACACAACGCGCCGACGTTGATCCACGGTTACCTCGGCTACGCCGATATCGTGCCGTCCG
CCACCGAGGAGCGCGACCTTTCCCTGGCCGCGCTCAAGCAGGCCCTACACGGCTAG

Upstream 100 bases:

>100_bases
GACGCAACCGCGGATCCACCACCGCACGGTCGCCGATGTGCGCTACCCAGTGTGGCCCGTCCCGGGTAAGTCCCCGAGGG
GATGCCGCTAAGGTGAGGCC

Downstream 100 bases:

>100_bases
ACCGGGACCTGCCACGCCCCAACAGGTACATGAGTACGCCGCCCAGCAGGGCGCCGACCGGCCAGGAAAGGGCGGTCACG
CCCTGGGCTCGCGGCACCAG

Product: lipase LipH

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MTATAGIDPIIVKILEAVPIQLVSPDGPQASRDAYRAIATARPPWIEVANIEDRTIPGPAGEIPVRIYSPAEGGVRPVFV
LIHGGGWVIGDLDTHDAVARAAAVQADAVVVSVDYRLAPEHPYPAAVEDCWAALQWVHEHAAEIGGDPGRLAVGGDSAGG
NLSAVMAQLARDNGIDLAFQVLWYPATTFDTTLPSMVENAEAPVLDFKTVGALSKWYLGDTNPATAGPTLVPARAENFEG
LAPAFIGTAQFDPLRDDGAKYAELLRDAGVPVQLHNAPTLIHGYLGYADIVPSATEERDLSLAALKQALHG

Sequences:

>Translated_311_residues
MTATAGIDPIIVKILEAVPIQLVSPDGPQASRDAYRAIATARPPWIEVANIEDRTIPGPAGEIPVRIYSPAEGGVRPVFV
LIHGGGWVIGDLDTHDAVARAAAVQADAVVVSVDYRLAPEHPYPAAVEDCWAALQWVHEHAAEIGGDPGRLAVGGDSAGG
NLSAVMAQLARDNGIDLAFQVLWYPATTFDTTLPSMVENAEAPVLDFKTVGALSKWYLGDTNPATAGPTLVPARAENFEG
LAPAFIGTAQFDPLRDDGAKYAELLRDAGVPVQLHNAPTLIHGYLGYADIVPSATEERDLSLAALKQALHG
>Mature_310_residues
TATAGIDPIIVKILEAVPIQLVSPDGPQASRDAYRAIATARPPWIEVANIEDRTIPGPAGEIPVRIYSPAEGGVRPVFVL
IHGGGWVIGDLDTHDAVARAAAVQADAVVVSVDYRLAPEHPYPAAVEDCWAALQWVHEHAAEIGGDPGRLAVGGDSAGGN
LSAVMAQLARDNGIDLAFQVLWYPATTFDTTLPSMVENAEAPVLDFKTVGALSKWYLGDTNPATAGPTLVPARAENFEGL
APAFIGTAQFDPLRDDGAKYAELLRDAGVPVQLHNAPTLIHGYLGYADIVPSATEERDLSLAALKQALHG

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=292, Percent_Identity=31.1643835616438, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI206597554, Length=289, Percent_Identity=33.2179930795848, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI157041239, Length=291, Percent_Identity=27.1477663230241, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI61966717, Length=324, Percent_Identity=24.6913580246914, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI21328446, Length=146, Percent_Identity=33.5616438356164, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI68051721, Length=155, Percent_Identity=34.8387096774194, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI226423947, Length=163, Percent_Identity=33.1288343558282, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI68508957, Length=114, Percent_Identity=36.8421052631579, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI68508967, Length=114, Percent_Identity=36.8421052631579, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI68508965, Length=114, Percent_Identity=36.8421052631579, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI297747275, Length=110, Percent_Identity=41.8181818181818, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1786682, Length=244, Percent_Identity=28.2786885245902, Blast_Score=110, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17567059, Length=299, Percent_Identity=27.4247491638796, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17540028, Length=311, Percent_Identity=24.7588424437299, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI71996133, Length=127, Percent_Identity=36.2204724409449, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI72001146, Length=128, Percent_Identity=38.28125, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI115533410, Length=80, Percent_Identity=38.75, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI115533412, Length=81, Percent_Identity=38.2716049382716, Blast_Score=64, Evalue=1e-10,
Organism=Drosophila melanogaster, GI24656084, Length=98, Percent_Identity=35.7142857142857, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24656076, Length=98, Percent_Identity=35.7142857142857, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI20130169, Length=98, Percent_Identity=35.7142857142857, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 32725; Mature: 32594

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATAGIDPIIVKILEAVPIQLVSPDGPQASRDAYRAIATARPPWIEVANIEDRTIPGPA
CCCCCCCCHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCC
GEIPVRIYSPAEGGVRPVFVLIHGGGWVIGDLDTHDAVARAAAVQADAVVVSVDYRLAPE
CCCCEEEECCCCCCCCEEEEEEECCCEEEECCCCHHHHHHHHHHCCCEEEEEEEEEECCC
HPYPAAVEDCWAALQWVHEHAAEIGGDPGRLAVGGDSAGGNLSAVMAQLARDNGIDLAFQ
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCEEEE
VLWYPATTFDTTLPSMVENAEAPVLDFKTVGALSKWYLGDTNPATAGPTLVPARAENFEG
EEEECCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCC
LAPAFIGTAQFDPLRDDGAKYAELLRDAGVPVQLHNAPTLIHGYLGYADIVPSATEERDL
CCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCEEEHHHCHHHHCCCCCCHHCH
SLAALKQALHG
HHHHHHHHHCC
>Mature Secondary Structure 
TATAGIDPIIVKILEAVPIQLVSPDGPQASRDAYRAIATARPPWIEVANIEDRTIPGPA
CCCCCCCHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCC
GEIPVRIYSPAEGGVRPVFVLIHGGGWVIGDLDTHDAVARAAAVQADAVVVSVDYRLAPE
CCCCEEEECCCCCCCCEEEEEEECCCEEEECCCCHHHHHHHHHHCCCEEEEEEEEEECCC
HPYPAAVEDCWAALQWVHEHAAEIGGDPGRLAVGGDSAGGNLSAVMAQLARDNGIDLAFQ
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCEEEE
VLWYPATTFDTTLPSMVENAEAPVLDFKTVGALSKWYLGDTNPATAGPTLVPARAENFEG
EEEECCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCC
LAPAFIGTAQFDPLRDDGAKYAELLRDAGVPVQLHNAPTLIHGYLGYADIVPSATEERDL
CCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCEEEHHHCHHHHCCCCCCHHCH
SLAALKQALHG
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]