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The map label for this gene is ansA [H]

Identifier: 169629786

GI number: 169629786

Start: 2745993

End: 2746970

Strand: Direct

Name: ansA [H]

Synonym: MAB_2701

Alternate gene names: 169629786

Gene position: 2745993-2746970 (Clockwise)

Preceding gene: 169629780

Following gene: 169629788

Centisome position: 54.19

GC content: 70.04

Gene sequence:

>978_bases
ATGATCGCCGCTGTGCCTGCCGCTCTTCGCCCAAATGACTCCTCGCCAGCGCTCGTTCTCTTGACCACGGGGGGCACGAT
CGCGACCGAGGTGGGCGCAGACGGCATCGCCCGGCATGCCAGTTCCGGCGGCGATCTGCTGGCGGCAAGTGGCCAGGGCA
ATGTGCTGGTCGAGGACCTGATGACGATCGACAGCGCGGAGATGACGCCGCAGCGCTGGCAGCAGATCGCGGCCTCGGTA
CGCGGGCATGTCGCCGCGGGCGCGACCGGAATCGTCATCGCACACGGCACGGACACTCTGGAAGAAACCGCTTTGTGGCT
CGCGCTGACATGCGCGGTGCCGGTCCCCGTCGTACTGACCGGCGCACAACGCAGCGGCGACCACCCCGAGTCCGATGGTC
CAGGCAACCTGCGCGACGCGTTGACGGTGGCGGCCTCCGGCGAGGCGCTGGGCGTGGTGGTGTGCTTCGCCGGGCAGGTC
TTTCCGGCGGCGGGCATCCGCAAGGTGGATCTGACCGCGACCGCGGGATTCGCCGGGGCACCGCCGGTCGGGCAGATACG
CGACGGCGCCTTCGTCCGCACCGCCCAGGCGTCCTCGTCGTTTCTGGGCACCGTGACCCGTCCGGCGCTGCCGCGGGTCG
ATGTGGTGAGCCTGTACCCGGGTGCCGACGCCGTGGCACTGGACGCCTTCGCGCGGGCCGGCGCGCAGGGCCTAGTGCTC
GAATCGATGGGTGCCGGAAACGCCAACGACGCTGTCACCGAAGCGGTTTCCCGCCACATCGAGAACGGGATACATGTTGT
GGTGACCACCAGGGTGCCGGGTGGCCTGCTGTCCCCGGGCTACGCACCGGGACAGAAGCTCATCGACGCCGGCGCGGTGG
TCGCGCCTCGGCTTCGCGCCGGCCAAGCGCGAGTGCTGTTGATGGCGGCCCTAGCCACCGGTTCCGATCTCGTCGCCGTC
ATCGACCGGCTCGGCTGA

Upstream 100 bases:

>100_bases
AGTTTTATCCGCCGCTTTGGCTTCCGGGCGGTCTCGGACTCGTCACTCACGTCCCCCATCATCCCTTACGCGGCGCAGCG
CTTTGCGCAACCTGTCTGCG

Downstream 100 bases:

>100_bases
CTCCCCCGGCCAGTCCAGGCTCGCCACCGGGTCGGCGCGCGCGATACCCGGATCATCGACCGCGAAGGTGCGCGCGATAC
CCGGACCGCTGCTTCGCGTC

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MIAAVPAALRPNDSSPALVLLTTGGTIATEVGADGIARHASSGGDLLAASGQGNVLVEDLMTIDSAEMTPQRWQQIAASV
RGHVAAGATGIVIAHGTDTLEETALWLALTCAVPVPVVLTGAQRSGDHPESDGPGNLRDALTVAASGEALGVVVCFAGQV
FPAAGIRKVDLTATAGFAGAPPVGQIRDGAFVRTAQASSSFLGTVTRPALPRVDVVSLYPGADAVALDAFARAGAQGLVL
ESMGAGNANDAVTEAVSRHIENGIHVVVTTRVPGGLLSPGYAPGQKLIDAGAVVAPRLRAGQARVLLMAALATGSDLVAV
IDRLG

Sequences:

>Translated_325_residues
MIAAVPAALRPNDSSPALVLLTTGGTIATEVGADGIARHASSGGDLLAASGQGNVLVEDLMTIDSAEMTPQRWQQIAASV
RGHVAAGATGIVIAHGTDTLEETALWLALTCAVPVPVVLTGAQRSGDHPESDGPGNLRDALTVAASGEALGVVVCFAGQV
FPAAGIRKVDLTATAGFAGAPPVGQIRDGAFVRTAQASSSFLGTVTRPALPRVDVVSLYPGADAVALDAFARAGAQGLVL
ESMGAGNANDAVTEAVSRHIENGIHVVVTTRVPGGLLSPGYAPGQKLIDAGAVVAPRLRAGQARVLLMAALATGSDLVAV
IDRLG
>Mature_325_residues
MIAAVPAALRPNDSSPALVLLTTGGTIATEVGADGIARHASSGGDLLAASGQGNVLVEDLMTIDSAEMTPQRWQQIAASV
RGHVAAGATGIVIAHGTDTLEETALWLALTCAVPVPVVLTGAQRSGDHPESDGPGNLRDALTVAASGEALGVVVCFAGQV
FPAAGIRKVDLTATAGFAGAPPVGQIRDGAFVRTAQASSSFLGTVTRPALPRVDVVSLYPGADAVALDAFARAGAQGLVL
ESMGAGNANDAVTEAVSRHIENGIHVVVTTRVPGGLLSPGYAPGQKLIDAGAVVAPRLRAGQARVLLMAALATGSDLVAV
IDRLG

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=230, Percent_Identity=27.8260869565217, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1789327, Length=274, Percent_Identity=31.7518248175182, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788065, Length=241, Percent_Identity=32.3651452282158, Blast_Score=95, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI193207372, Length=248, Percent_Identity=27.0161290322581, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193207374, Length=248, Percent_Identity=27.0161290322581, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17558280, Length=248, Percent_Identity=27.0161290322581, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17558278, Length=248, Percent_Identity=27.0161290322581, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320527, Length=325, Percent_Identity=27.0769230769231, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6323189, Length=346, Percent_Identity=26.5895953757225, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323187, Length=346, Percent_Identity=26.5895953757225, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323186, Length=346, Percent_Identity=26.5895953757225, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323184, Length=346, Percent_Identity=26.5895953757225, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24645530, Length=256, Percent_Identity=28.90625, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24639609, Length=243, Percent_Identity=26.3374485596708, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 32457; Mature: 32457

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAAVPAALRPNDSSPALVLLTTGGTIATEVGADGIARHASSGGDLLAASGQGNVLVEDL
CCCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCHHHHHCCCCCCEEEECCCCCEEHHHH
MTIDSAEMTPQRWQQIAASVRGHVAAGATGIVIAHGTDTLEETALWLALTCAVPVPVVLT
HHCCCCCCCHHHHHHHHHHHCCCEECCCCEEEEECCCCCHHHHHHHHEEEHHCCCCEEEE
GAQRSGDHPESDGPGNLRDALTVAASGEALGVVVCFAGQVFPAAGIRKVDLTATAGFAGA
CCCCCCCCCCCCCCCCHHHHEEEEECCCEEEEEEEECCCCCCCCCCEEEEEEEECCCCCC
PPVGQIRDGAFVRTAQASSSFLGTVTRPALPRVDVVSLYPGADAVALDAFARAGAQGLVL
CCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEE
ESMGAGNANDAVTEAVSRHIENGIHVVVTTRVPGGLLSPGYAPGQKLIDAGAVVAPRLRA
EECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHCCCEECCCCCC
GQARVLLMAALATGSDLVAVIDRLG
CCHHEEEEEHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MIAAVPAALRPNDSSPALVLLTTGGTIATEVGADGIARHASSGGDLLAASGQGNVLVEDL
CCCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCHHHHHCCCCCCEEEECCCCCEEHHHH
MTIDSAEMTPQRWQQIAASVRGHVAAGATGIVIAHGTDTLEETALWLALTCAVPVPVVLT
HHCCCCCCCHHHHHHHHHHHCCCEECCCCEEEEECCCCCHHHHHHHHEEEHHCCCCEEEE
GAQRSGDHPESDGPGNLRDALTVAASGEALGVVVCFAGQVFPAAGIRKVDLTATAGFAGA
CCCCCCCCCCCCCCCCHHHHEEEEECCCEEEEEEEECCCCCCCCCCEEEEEEEECCCCCC
PPVGQIRDGAFVRTAQASSSFLGTVTRPALPRVDVVSLYPGADAVALDAFARAGAQGLVL
CCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEE
ESMGAGNANDAVTEAVSRHIENGIHVVVTTRVPGGLLSPGYAPGQKLIDAGAVVAPRLRA
EECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHCCCEECCCCCC
GQARVLLMAALATGSDLVAVIDRLG
CCHHEEEEEHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]