Click here to switch to the map view.

The map label for this gene is nfdA [H]

Identifier: 169629632

GI number: 169629632

Start: 2593415

End: 2595241

Strand: Direct

Name: nfdA [H]

Synonym: MAB_2546

Alternate gene names: 169629632

Gene position: 2593415-2595241 (Clockwise)

Preceding gene: 169629628

Following gene: 169629635

Centisome position: 51.18

GC content: 62.45

Gene sequence:

>1827_bases
ATGTGCACCGCATGTGAGTGGGCCCCACACTTCGCGGCGCTCAGCGCCAAGAGCACCCGCCGGACCGCACTGCGTGCGGC
CGTGGCGCTCACGCTCGCGGCGGGCGCTGCCGCATGTTCGGCAGGGACAGTTCCGGCACCCACGACAGGTAGTGCCGGCC
CGCAGAACACCCACGCGGACTTCGTGTTCCGCAACGGGCGCGTCTATACGGTGGCCGGCCCCACGGAATGGGCCCAAGCG
GTTGCCGTACAAGGCAATGCCATCACGCACGTCGGTGACGAGGCGGGTGCGATGGCATTGGTGGGCCCAAACACTAGAGT
GGTGGATCTTGCGGGCAGGCTATTGATGCCCGGCTTCGTCGAAGGTCATATCCACCCTTTCTTCGGTGCCTTTCTGAGTG
GTGGTGTGAATCTGCAAGTTTCGACCAAAGAGGCGGCGCTGGCAGCTATCGCCAAGTACGCCGAGGAAAATCCGGTTGGT
CCCGTACGCGGATTTGGCTGGCGCGTCGACATGTTCGGGCCGGAAGGTCCAACCCGTCAGGAATTGGACAGCGTGCTGCC
CGATCGTCCCGGATTCTTCTTCGCTGTCGACGCCCACAGTATGTGGGCCAACAGCAAGAGCCTTGAGGTTGCCGGAATCA
ATCGTGAAACCCCAGATCCGGTACCGGGTTTCAGCTATTACGCAAAAGACAGCCATGGCGAACCAACCGGTTTCATCTTG
GAGATCAGTGCGATACTGGACTTGGTCAATGCGATCGAGCCGGTGACCGCACAGAGCCTGCAGCGAGCCTTAGAGGACTG
GCTGCCCAATGCCGCGGCAGCGGGCATTACGTCGGTTTTCGATGCCGGTGTTCCGGCTGTCGCCAGTCAGGCAGCTCTTA
TCGGGTTATATGGCGACATTGAGCGTAGGGGCAGATTGCCGTTTCGTGTGGTGGCGTCCTATACCGTCAAAGCGCCCCCG
TTTGGCGACGTGGTGGCGAAGTTCGCCGAGACGCGCGACCAGGTATCCACTGACCTGGTTCGCGTGGGCGCAGTCAAGAT
CATGGGCGACGGCACGCAGGGCGGCTACACCGCGTGGCTCATCGATCCCTATGCCGACAAGCCCGATTCAATTGGTACGT
CGCCGTTCACCGAGCAAGAGTGGGGCGAGCTGGTCGGCCGAATCGACTCGGCGGGAATTGATATGCACGTGCATGCGTGC
GGCGAGCGCACCGCCCGGGTGGCGCTGGATGCGTTCGAGGCGGCTATCGCGAACAACCCGGCCCGCGACCGCCGCCATAC
GGTTGCGCACCTGATCTACGTCGATGACGCGGACAGTGCGCGGTTCGCCAAACTTGGTGTTGTTGCGCAGTTCTCGGCGA
ACTGGTTCTCCGCCGACCCTGACACCGTGGTCAACATGGCAGCCCGCTACGGCGCGCCTCGCAAGGACAACTTCTACCGG
ATCCAGGATATTCTCCGCACGGGCGCGCACGTGGCCTTGGGAACGGACTGGCCGGCGGCGGGCTACTTCTCCACATACAA
GCCACTCGAATCCATCCAAATCGGTGTCACCCGTCAGCTCATCGGCGATCCCGGTGCCCAAGTGTTGCGCCCCCATGATC
AGAAGTTATCGGTGGCCGAGGCGGTGCGCGCTAACACGCTGGGTGCCGCTTACCAGATCCGGATGGACAACAAGGTGGGC
TCCGTAGAGGTCGGCAAGCTCGCTGATCTCATCGTGCTCGACCGCAATATCCTCGAGTGCGACCCACAGGACATCCACAC
GGCCACCGTGTTGATGACCATGATGAACGGCCAGGTCCGCCATGAGCTCCAGGGTCTCAAGAGCTGA

Upstream 100 bases:

>100_bases
TTGGTAGGCGGTGGGGTAGGTGTGCCGCCTCCGCATGATCGCCATTACGGTGACGGTAGAACAAACTGAAAATTCAGTCA
AGATTTGATATGGTGCCTCT

Downstream 100 bases:

>100_bases
GCGGCGTTGTGGCGGAGTGACTTACCAGCGCTCGCCGTTATGTGGCCAATACTAGGAGCGGCTTGTCCTTATCGACTACA
TAGGTCGCCAGTTCCGACCC

Product: amidohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 608; Mature: 608

Protein sequence:

>608_residues
MCTACEWAPHFAALSAKSTRRTALRAAVALTLAAGAAACSAGTVPAPTTGSAGPQNTHADFVFRNGRVYTVAGPTEWAQA
VAVQGNAITHVGDEAGAMALVGPNTRVVDLAGRLLMPGFVEGHIHPFFGAFLSGGVNLQVSTKEAALAAIAKYAEENPVG
PVRGFGWRVDMFGPEGPTRQELDSVLPDRPGFFFAVDAHSMWANSKSLEVAGINRETPDPVPGFSYYAKDSHGEPTGFIL
EISAILDLVNAIEPVTAQSLQRALEDWLPNAAAAGITSVFDAGVPAVASQAALIGLYGDIERRGRLPFRVVASYTVKAPP
FGDVVAKFAETRDQVSTDLVRVGAVKIMGDGTQGGYTAWLIDPYADKPDSIGTSPFTEQEWGELVGRIDSAGIDMHVHAC
GERTARVALDAFEAAIANNPARDRRHTVAHLIYVDDADSARFAKLGVVAQFSANWFSADPDTVVNMAARYGAPRKDNFYR
IQDILRTGAHVALGTDWPAAGYFSTYKPLESIQIGVTRQLIGDPGAQVLRPHDQKLSVAEAVRANTLGAAYQIRMDNKVG
SVEVGKLADLIVLDRNILECDPQDIHTATVLMTMMNGQVRHELQGLKS

Sequences:

>Translated_608_residues
MCTACEWAPHFAALSAKSTRRTALRAAVALTLAAGAAACSAGTVPAPTTGSAGPQNTHADFVFRNGRVYTVAGPTEWAQA
VAVQGNAITHVGDEAGAMALVGPNTRVVDLAGRLLMPGFVEGHIHPFFGAFLSGGVNLQVSTKEAALAAIAKYAEENPVG
PVRGFGWRVDMFGPEGPTRQELDSVLPDRPGFFFAVDAHSMWANSKSLEVAGINRETPDPVPGFSYYAKDSHGEPTGFIL
EISAILDLVNAIEPVTAQSLQRALEDWLPNAAAAGITSVFDAGVPAVASQAALIGLYGDIERRGRLPFRVVASYTVKAPP
FGDVVAKFAETRDQVSTDLVRVGAVKIMGDGTQGGYTAWLIDPYADKPDSIGTSPFTEQEWGELVGRIDSAGIDMHVHAC
GERTARVALDAFEAAIANNPARDRRHTVAHLIYVDDADSARFAKLGVVAQFSANWFSADPDTVVNMAARYGAPRKDNFYR
IQDILRTGAHVALGTDWPAAGYFSTYKPLESIQIGVTRQLIGDPGAQVLRPHDQKLSVAEAVRANTLGAAYQIRMDNKVG
SVEVGKLADLIVLDRNILECDPQDIHTATVLMTMMNGQVRHELQGLKS
>Mature_608_residues
MCTACEWAPHFAALSAKSTRRTALRAAVALTLAAGAAACSAGTVPAPTTGSAGPQNTHADFVFRNGRVYTVAGPTEWAQA
VAVQGNAITHVGDEAGAMALVGPNTRVVDLAGRLLMPGFVEGHIHPFFGAFLSGGVNLQVSTKEAALAAIAKYAEENPVG
PVRGFGWRVDMFGPEGPTRQELDSVLPDRPGFFFAVDAHSMWANSKSLEVAGINRETPDPVPGFSYYAKDSHGEPTGFIL
EISAILDLVNAIEPVTAQSLQRALEDWLPNAAAAGITSVFDAGVPAVASQAALIGLYGDIERRGRLPFRVVASYTVKAPP
FGDVVAKFAETRDQVSTDLVRVGAVKIMGDGTQGGYTAWLIDPYADKPDSIGTSPFTEQEWGELVGRIDSAGIDMHVHAC
GERTARVALDAFEAAIANNPARDRRHTVAHLIYVDDADSARFAKLGVVAQFSANWFSADPDTVVNMAARYGAPRKDNFYR
IQDILRTGAHVALGTDWPAAGYFSTYKPLESIQIGVTRQLIGDPGAQVLRPHDQKLSVAEAVRANTLGAAYQIRMDNKVG
SVEVGKLADLIVLDRNILECDPQDIHTATVLMTMMNGQVRHELQGLKS

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 64785; Mature: 64785

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCTACEWAPHFAALSAKSTRRTALRAAVALTLAAGAAACSAGTVPAPTTGSAGPQNTHAD
CCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
FVFRNGRVYTVAGPTEWAQAVAVQGNAITHVGDEAGAMALVGPNTRVVDLAGRLLMPGFV
EEEECCEEEEEECCHHHHHHEEECCCEEEECCCCCCEEEEECCCCEEEEHHCHHHCCCHH
EGHIHPFFGAFLSGGVNLQVSTKEAALAAIAKYAEENPVGPVRGFGWRVDMFGPEGPTRQ
CCCCCHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHH
ELDSVLPDRPGFFFAVDAHSMWANSKSLEVAGINRETPDPVPGFSYYAKDSHGEPTGFIL
HHHHHCCCCCCEEEEEECHHHHCCCCCEEEEECCCCCCCCCCCCCEECCCCCCCCCEEEE
EISAILDLVNAIEPVTAQSLQRALEDWLPNAAAAGITSVFDAGVPAVASQAALIGLYGDI
EHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHCCCEEEEEECCH
ERRGRLPFRVVASYTVKAPPFGDVVAKFAETRDQVSTDLVRVGAVKIMGDGTQGGYTAWL
HHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEE
IDPYADKPDSIGTSPFTEQEWGELVGRIDSAGIDMHVHACGERTARVALDAFEAAIANNP
ECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCC
ARDRRHTVAHLIYVDDADSARFAKLGVVAQFSANWFSADPDTVVNMAARYGAPRKDNFYR
CHHHHCEEEEEEEEECCCCCCEEHEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHH
IQDILRTGAHVALGTDWPAAGYFSTYKPLESIQIGVTRQLIGDPGAQVLRPHDQKLSVAE
HHHHHHCCCEEEECCCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHH
AVRANTLGAAYQIRMDNKVGSVEVGKLADLIVLDRNILECDPQDIHTATVLMTMMNGQVR
HHHHCCCCCEEEEEECCCCCCEEHHHHHEEEEECCCHHCCCCCHHHHHHHHHHHHCCHHH
HELQGLKS
HHHHCCCC
>Mature Secondary Structure
MCTACEWAPHFAALSAKSTRRTALRAAVALTLAAGAAACSAGTVPAPTTGSAGPQNTHAD
CCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
FVFRNGRVYTVAGPTEWAQAVAVQGNAITHVGDEAGAMALVGPNTRVVDLAGRLLMPGFV
EEEECCEEEEEECCHHHHHHEEECCCEEEECCCCCCEEEEECCCCEEEEHHCHHHCCCHH
EGHIHPFFGAFLSGGVNLQVSTKEAALAAIAKYAEENPVGPVRGFGWRVDMFGPEGPTRQ
CCCCCHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHH
ELDSVLPDRPGFFFAVDAHSMWANSKSLEVAGINRETPDPVPGFSYYAKDSHGEPTGFIL
HHHHHCCCCCCEEEEEECHHHHCCCCCEEEEECCCCCCCCCCCCCEECCCCCCCCCEEEE
EISAILDLVNAIEPVTAQSLQRALEDWLPNAAAAGITSVFDAGVPAVASQAALIGLYGDI
EHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHCCCEEEEEECCH
ERRGRLPFRVVASYTVKAPPFGDVVAKFAETRDQVSTDLVRVGAVKIMGDGTQGGYTAWL
HHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEE
IDPYADKPDSIGTSPFTEQEWGELVGRIDSAGIDMHVHACGERTARVALDAFEAAIANNP
ECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCC
ARDRRHTVAHLIYVDDADSARFAKLGVVAQFSANWFSADPDTVVNMAARYGAPRKDNFYR
CHHHHCEEEEEEEEECCCCCCEEHEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHH
IQDILRTGAHVALGTDWPAAGYFSTYKPLESIQIGVTRQLIGDPGAQVLRPHDQKLSVAE
HHHHHHCCCEEEECCCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHH
AVRANTLGAAYQIRMDNKVGSVEVGKLADLIVLDRNILECDPQDIHTATVLMTMMNGQVR
HHHHCCCCCEEEEEECCCCCCEEHHHHHEEEEECCCHHCCCCCHHHHHHHHHHHHCCHHH
HELQGLKS
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA