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The map label for this gene is camA [H]

Identifier: 169629134

GI number: 169629134

Start: 2047894

End: 2049093

Strand: Reverse

Name: camA [H]

Synonym: MAB_2047c

Alternate gene names: 169629134

Gene position: 2049093-2047894 (Counterclockwise)

Preceding gene: 169629135

Following gene: 169629129

Centisome position: 40.44

GC content: 63.5

Gene sequence:

>1200_bases
ATGACTCCCGAACGAGCGGTGATCGTCGGCGCGAGCCACGCCGGCGCGCAGTTGGCAGCTAATCTTCGAAGGGAGGGGTG
GTCTGGGGAGGTCGTGCTCATCGGCGACGAGGGGGGACTGCCCTACCACCGGCCTCCGTTGTCGAAGGGATACCTGGCCG
GCAAGAACGGCCTCGACGACCTCCTGATTCGCGGCGCTGATTTCTACGAAAAGCAGCACATTCGACTCTTGAATGCGACC
GTGGAGGCGATCCACCGGAGTGCCAAGCGTGTGTCTCTGAGCACCGGCGACACGCTGACGTACACCAAGCTCGCGTTGTG
CACCGGCGCAAGGGCCAGACGACTCCCCACACCAGGGGTGGATCTTCCCGGAATTCACTACCTGCGTACCGCTGCAGACG
TCGAGTTGATCCGTGCCGCCGCCACACCGGGTCGGAGGGTTGTGATCGTGGGCGGCGGTTACATCGGGTTGGAAACGGCG
GCCTCGCTGTGTTCGCTCGGCATGAACGTCACCGTCCTCGAGGCAACCGAGCGTGTACTCGAAAGGGTCACCGCGCCGGA
GGTTTCCGCGTTTTACACGCGAATCCACCGCGGCGAGGGAGTGGAGATCCGAACACACGCCCTCGTCGAAGCCTTCTCCG
GTAACGGTGGGGTGCAGGAGGTCGTGCTGGCTGACGGCGAATCGATCCCCGCCGACTTGGTCATCGTCGGCGTCGGTGTG
GTGCCGAACACCGAGCTCGCCTCGGCCGCAGGATTATCCGTCGACAACGGCATCGTGATCGACGACCAGGCCCGCACCAG
CGACCCCGACATCGTGGCCGCCGGCGACTGCACGAGCCACACCATGGCTCGATACGGCTCGCGTATTCGTTTGGAATCCG
TATCGAGCGCCGGCGAGCAGGCCAAGATCGCTGCGGCGACAATCTGCGGAAAACACAGCGCAATCGCTGCGCTTCCGTGG
TTCTGGTCCGACCAGTACGATCTCAAACTCCAGATTGCCGGTCTCAACGCCGGATACGACGAACTGTTGCTCAGTGGTGA
CCCGTCGCGTGACCGTGACTTCAGCTGCTTCTACTTCCGCGAGGGCGAACTCATCGCCGCCGACTGCGTCAATCGTCCTC
GCGATTTCATGTCCAGTAAGCGGGCCATCAGCCAGCAGCTCCGAGTTGACCGCTCAGAGCTCCTCGCCGGCTCGATCTGA

Upstream 100 bases:

>100_bases
TTGAGAACATCGAGGTGGTCGGCGAACCCGAGTATGTGCAATCGAATTTCGTCAGGGGTATCAGCAAGCTGATGGTTCGC
CTCACTCCCAGGCCGAGTGC

Downstream 100 bases:

>100_bases
CACGAGAGCTTGCTGGCTACAAACTGTGGACAACTCCCAGGTACCTGAGTGCTACGCGGGAGATCCGCTCACCGGTGGTG
GCGATGTCCGCGGCGGGGAG

Product: ferredoxin reductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTPERAVIVGASHAGAQLAANLRREGWSGEVVLIGDEGGLPYHRPPLSKGYLAGKNGLDDLLIRGADFYEKQHIRLLNAT
VEAIHRSAKRVSLSTGDTLTYTKLALCTGARARRLPTPGVDLPGIHYLRTAADVELIRAAATPGRRVVIVGGGYIGLETA
ASLCSLGMNVTVLEATERVLERVTAPEVSAFYTRIHRGEGVEIRTHALVEAFSGNGGVQEVVLADGESIPADLVIVGVGV
VPNTELASAAGLSVDNGIVIDDQARTSDPDIVAAGDCTSHTMARYGSRIRLESVSSAGEQAKIAAATICGKHSAIAALPW
FWSDQYDLKLQIAGLNAGYDELLLSGDPSRDRDFSCFYFREGELIAADCVNRPRDFMSSKRAISQQLRVDRSELLAGSI

Sequences:

>Translated_399_residues
MTPERAVIVGASHAGAQLAANLRREGWSGEVVLIGDEGGLPYHRPPLSKGYLAGKNGLDDLLIRGADFYEKQHIRLLNAT
VEAIHRSAKRVSLSTGDTLTYTKLALCTGARARRLPTPGVDLPGIHYLRTAADVELIRAAATPGRRVVIVGGGYIGLETA
ASLCSLGMNVTVLEATERVLERVTAPEVSAFYTRIHRGEGVEIRTHALVEAFSGNGGVQEVVLADGESIPADLVIVGVGV
VPNTELASAAGLSVDNGIVIDDQARTSDPDIVAAGDCTSHTMARYGSRIRLESVSSAGEQAKIAAATICGKHSAIAALPW
FWSDQYDLKLQIAGLNAGYDELLLSGDPSRDRDFSCFYFREGELIAADCVNRPRDFMSSKRAISQQLRVDRSELLAGSI
>Mature_398_residues
TPERAVIVGASHAGAQLAANLRREGWSGEVVLIGDEGGLPYHRPPLSKGYLAGKNGLDDLLIRGADFYEKQHIRLLNATV
EAIHRSAKRVSLSTGDTLTYTKLALCTGARARRLPTPGVDLPGIHYLRTAADVELIRAAATPGRRVVIVGGGYIGLETAA
SLCSLGMNVTVLEATERVLERVTAPEVSAFYTRIHRGEGVEIRTHALVEAFSGNGGVQEVVLADGESIPADLVIVGVGVV
PNTELASAAGLSVDNGIVIDDQARTSDPDIVAAGDCTSHTMARYGSRIRLESVSSAGEQAKIAAATICGKHSAIAALPWF
WSDQYDLKLQIAGLNAGYDELLLSGDPSRDRDFSCFYFREGELIAADCVNRPRDFMSSKRAISQQLRVDRSELLAGSI

Specific function: The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI65787454, Length=392, Percent_Identity=29.0816326530612, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI226437568, Length=392, Percent_Identity=29.0816326530612, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI21389617, Length=392, Percent_Identity=29.0816326530612, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI4757732, Length=333, Percent_Identity=25.2252252252252, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI22202629, Length=333, Percent_Identity=25.2252252252252, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1788892, Length=402, Percent_Identity=36.5671641791045, Blast_Score=184, Evalue=9e-48,
Organism=Escherichia coli, GI1789065, Length=272, Percent_Identity=31.25, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789765, Length=345, Percent_Identity=28.1159420289855, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559934, Length=368, Percent_Identity=30.7065217391304, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI32564386, Length=390, Percent_Identity=25.3846153846154, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24639257, Length=367, Percent_Identity=29.4277929155313, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI281359715, Length=367, Percent_Identity=29.4277929155313, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI281359713, Length=367, Percent_Identity=29.4277929155313, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24639250, Length=367, Percent_Identity=29.4277929155313, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI18543267, Length=367, Percent_Identity=29.4277929155313, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24639252, Length=367, Percent_Identity=29.4277929155313, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24585130, Length=367, Percent_Identity=23.7057220708447, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI28573993, Length=356, Percent_Identity=23.876404494382, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24581020, Length=356, Percent_Identity=23.876404494382, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 42518; Mature: 42386

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPERAVIVGASHAGAQLAANLRREGWSGEVVLIGDEGGLPYHRPPLSKGYLAGKNGLDD
CCCCCEEEEECCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEECCCCCHHH
LLIRGADFYEKQHIRLLNATVEAIHRSAKRVSLSTGDTLTYTKLALCTGARARRLPTPGV
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEHHHHHHHCCCHHCCCCCCCC
DLPGIHYLRTAADVELIRAAATPGRRVVIVGGGYIGLETAASLCSLGMNVTVLEATERVL
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEECHHHHHHHHHCCCCEEEEHHHHHHH
ERVTAPEVSAFYTRIHRGEGVEIRTHALVEAFSGNGGVQEVVLADGESIPADLVIVGVGV
HHHCCCHHHHHHHHHHCCCCCEEEHHEEEEECCCCCCEEEEEEECCCCCCCEEEEEEECC
VPNTELASAAGLSVDNGIVIDDQARTSDPDIVAAGDCTSHTMARYGSRIRLESVSSAGEQ
CCCCCHHHHCCCCCCCCEEEECCCCCCCCCEEEECCCCHHHHHHCCCEEEEEHHHCCCCC
AKIAAATICGKHSAIAALPWFWSDQYDLKLQIAGLNAGYDELLLSGDPSRDRDFSCFYFR
CEEEEEECCCCCCCEEEECEEECCCEEEEEEEEECCCCHHHHEECCCCCCCCCCEEEEEE
EGELIAADCVNRPRDFMSSKRAISQQLRVDRSELLAGSI
CCCEEEHHHCCCCHHHHHHHHHHHHHHHCCHHHHHCCCC
>Mature Secondary Structure 
TPERAVIVGASHAGAQLAANLRREGWSGEVVLIGDEGGLPYHRPPLSKGYLAGKNGLDD
CCCCEEEEECCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEECCCCCHHH
LLIRGADFYEKQHIRLLNATVEAIHRSAKRVSLSTGDTLTYTKLALCTGARARRLPTPGV
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEHHHHHHHCCCHHCCCCCCCC
DLPGIHYLRTAADVELIRAAATPGRRVVIVGGGYIGLETAASLCSLGMNVTVLEATERVL
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEECHHHHHHHHHCCCCEEEEHHHHHHH
ERVTAPEVSAFYTRIHRGEGVEIRTHALVEAFSGNGGVQEVVLADGESIPADLVIVGVGV
HHHCCCHHHHHHHHHHCCCCCEEEHHEEEEECCCCCCEEEEEEECCCCCCCEEEEEEECC
VPNTELASAAGLSVDNGIVIDDQARTSDPDIVAAGDCTSHTMARYGSRIRLESVSSAGEQ
CCCCCHHHHCCCCCCCCEEEECCCCCCCCCEEEECCCCHHHHHHCCCEEEEEHHHCCCCC
AKIAAATICGKHSAIAALPWFWSDQYDLKLQIAGLNAGYDELLLSGDPSRDRDFSCFYFR
CEEEEEECCCCCCCEEEECEEECCCEEEEEEEEECCCCHHHHEECCCCCCCCCCEEEEEE
EGELIAADCVNRPRDFMSSKRAISQQLRVDRSELLAGSI
CCCEEEHHHCCCCHHHHHHHHHHHHHHHCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2180940; 2613690; 3003058; 12011076; 11524002 [H]