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The map label for this gene is cysNC [H]

Identifier: 169628749

GI number: 169628749

Start: 1686717

End: 1688009

Strand: Reverse

Name: cysNC [H]

Synonym: MAB_1659c

Alternate gene names: 169628749

Gene position: 1688009-1686717 (Counterclockwise)

Preceding gene: 169628750

Following gene: 169628748

Centisome position: 33.31

GC content: 65.97

Gene sequence:

>1293_bases
ATGAGCGATCTTCTGCGCATTGCCACCGCCGGCAGTGTGGACGACGGAAAGTCCACTCTGGTGGGCCGGCTGCTGTACGA
CACCAAGTCGGTGCTCATCGACCAGTTCGATGCCGTCACAAAGGCTTCCGAGTCGCGTGGACTCGACACTCCCGACCTGT
CACTACTTGTCGACGGGCTGCGCGCCGAACGCGAGCAGGGCATCACCATCGACGTGGCGTACCGCTACTTCGCGACACCC
AAGCGCTCGTTCATCCTCGCCGACACCCCGGGCCACGTGCAGTACACCCGGAACACGGTGTCCGGCGCCTCGACCGCGCA
ACTGGTGATCCTGCTGGTTGACGCCCGCAAGGGTGTCATCGAGCAGACCCGCCGGCACGCGGCGGTGCTGGCGCTCTTGG
GTGTGCCGCGGCTGGTGTTGGCGGTCAACAAGATCGACCTGGTGCCCGACAGCGCCCGCATCTTCGAGGCCATCAGCGCC
GAATTCAACGAGCTGACCAGCTCGTTGGGCTGGAAGCCCGAGAACGTGGTCGAGATCCCGGTCTCGGCACTGCACGGCGA
CAATATCGCCACCCGCAGCGAGCGCACCGGCTTCTACAACGGGCCCACCCTCATCGAGCACCTGGAATCGGTTCCGGCGC
ATGGTGAATCCGACGGAGCGTCCGGCACCATCGGTCTGCGGTTCCCCGTCCAATACGTGATCCGCCCGCGCACCGCCGAG
TACCCCGATTACCGCGGGTATGCCGGTCAGGTGGCCGCGGGTGCCGTGCGGGTGGGCGATGAGGTGGTGATCTCCCCCGC
CGGGCAGCGCACCACGGTCGCGGGCATCGACACCGCCGATGGGCCGCTTGAGGTGGCCGAGGCCGGCCGCAGCATCACCC
TGATTCTCGCCGATGACGTGGACGCCTCGCGGGGAGACCTGATCGCATCTCTGGACGTGCCGGATCCGGTTTCCGAGCTC
GACGGGACCGTGTGCTGGCTGGCCGACAAACCGTTGAAGGCCGGCGCACGCCTGCTACTCAAGCACGGCACCCGTACGGT
TCCCGCGATCATCGGTGCGCTGGTGGAGCGCTTCGACGAGCAGAACTTGTCCGCCGATCCTGCACCGGAGTCATTGGAAC
TCAACGACATCGGACAGATCAGCATTCGACTGGCCGAGGCCATCAGCGTGGATGAGTACGTCGACGACCGGACGGCGGGC
AGCTTCTTGCTGATCGATCCGTCCAGCGGTAACACGCTGGCGGCTGGGCTGGTCGGTAATGCCCTGCGCGCCGTCGAACT
GAATCGTGCGTAA

Upstream 100 bases:

>100_bases
TGGCTTCCTCCCGGATCACCGAACGCGGCGCCACCCGTGGCGATGACCGAGTGTCCGAGGCGGCGATGGAAGACCGCAAG
CGAGAGGGTTATTTCTGATC

Downstream 100 bases:

>100_bases
TCCGACGCTCGTCGCCGTCGCGCACGGGAGCCGCGATGCGCGGTTCGCGCAGAATGCGCGCCGAGTGGTCCGACAGGTGC
GCTCGCTACGGCCGGATCTG

Product: CysN/CysC bifunctional enzyme

Products: NA

Alternate protein names: Sulfate adenylyltransferase subunit 1; ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; APS kinase; ATP adenosine-5'-phosphosulfate 3'-phosphotransferase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSDLLRIATAGSVDDGKSTLVGRLLYDTKSVLIDQFDAVTKASESRGLDTPDLSLLVDGLRAEREQGITIDVAYRYFATP
KRSFILADTPGHVQYTRNTVSGASTAQLVILLVDARKGVIEQTRRHAAVLALLGVPRLVLAVNKIDLVPDSARIFEAISA
EFNELTSSLGWKPENVVEIPVSALHGDNIATRSERTGFYNGPTLIEHLESVPAHGESDGASGTIGLRFPVQYVIRPRTAE
YPDYRGYAGQVAAGAVRVGDEVVISPAGQRTTVAGIDTADGPLEVAEAGRSITLILADDVDASRGDLIASLDVPDPVSEL
DGTVCWLADKPLKAGARLLLKHGTRTVPAIIGALVERFDEQNLSADPAPESLELNDIGQISIRLAEAISVDEYVDDRTAG
SFLLIDPSSGNTLAAGLVGNALRAVELNRA

Sequences:

>Translated_430_residues
MSDLLRIATAGSVDDGKSTLVGRLLYDTKSVLIDQFDAVTKASESRGLDTPDLSLLVDGLRAEREQGITIDVAYRYFATP
KRSFILADTPGHVQYTRNTVSGASTAQLVILLVDARKGVIEQTRRHAAVLALLGVPRLVLAVNKIDLVPDSARIFEAISA
EFNELTSSLGWKPENVVEIPVSALHGDNIATRSERTGFYNGPTLIEHLESVPAHGESDGASGTIGLRFPVQYVIRPRTAE
YPDYRGYAGQVAAGAVRVGDEVVISPAGQRTTVAGIDTADGPLEVAEAGRSITLILADDVDASRGDLIASLDVPDPVSEL
DGTVCWLADKPLKAGARLLLKHGTRTVPAIIGALVERFDEQNLSADPAPESLELNDIGQISIRLAEAISVDEYVDDRTAG
SFLLIDPSSGNTLAAGLVGNALRAVELNRA
>Mature_429_residues
SDLLRIATAGSVDDGKSTLVGRLLYDTKSVLIDQFDAVTKASESRGLDTPDLSLLVDGLRAEREQGITIDVAYRYFATPK
RSFILADTPGHVQYTRNTVSGASTAQLVILLVDARKGVIEQTRRHAAVLALLGVPRLVLAVNKIDLVPDSARIFEAISAE
FNELTSSLGWKPENVVEIPVSALHGDNIATRSERTGFYNGPTLIEHLESVPAHGESDGASGTIGLRFPVQYVIRPRTAEY
PDYRGYAGQVAAGAVRVGDEVVISPAGQRTTVAGIDTADGPLEVAEAGRSITLILADDVDASRGDLIASLDVPDPVSELD
GTVCWLADKPLKAGARLLLKHGTRTVPAIIGALVERFDEQNLSADPAPESLELNDIGQISIRLAEAISVDEYVDDRTAGS
FLLIDPSSGNTLAAGLVGNALRAVELNRA

Specific function: APS kinase catalyzes the synthesis of activated sulfate [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the APS kinase family [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=433, Percent_Identity=28.8683602771363, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI5729864, Length=431, Percent_Identity=29.0023201856148, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI4503471, Length=444, Percent_Identity=26.5765765765766, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI46094014, Length=439, Percent_Identity=25.7403189066059, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI4503475, Length=446, Percent_Identity=26.2331838565022, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI194018520, Length=434, Percent_Identity=25.5760368663594, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI194018522, Length=434, Percent_Identity=25.5760368663594, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI194097354, Length=434, Percent_Identity=25.5760368663594, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI34147630, Length=298, Percent_Identity=28.5234899328859, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1789108, Length=420, Percent_Identity=46.4285714285714, Blast_Score=338, Evalue=5e-94,
Organism=Escherichia coli, GI2367247, Length=318, Percent_Identity=27.6729559748428, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789737, Length=143, Percent_Identity=32.1678321678322, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1790412, Length=143, Percent_Identity=32.1678321678322, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17552884, Length=444, Percent_Identity=26.5765765765766, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17569207, Length=444, Percent_Identity=26.5765765765766, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI115532067, Length=433, Percent_Identity=29.0993071593533, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=325, Percent_Identity=28.9230769230769, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=433, Percent_Identity=29.0993071593533, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32566303, Length=437, Percent_Identity=24.4851258581236, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI32566301, Length=166, Percent_Identity=33.7349397590361, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI25141371, Length=290, Percent_Identity=26.551724137931, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556456, Length=169, Percent_Identity=31.3609467455621, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6322937, Length=447, Percent_Identity=28.4116331096197, Blast_Score=156, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6325337, Length=441, Percent_Identity=26.530612244898, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6319594, Length=441, Percent_Identity=26.530612244898, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6320377, Length=438, Percent_Identity=26.4840182648402, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6324761, Length=180, Percent_Identity=32.2222222222222, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45550900, Length=436, Percent_Identity=27.0642201834862, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24652838, Length=311, Percent_Identity=31.1897106109325, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI17137572, Length=311, Percent_Identity=31.1897106109325, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI45553807, Length=206, Percent_Identity=34.4660194174757, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI45553816, Length=206, Percent_Identity=34.4660194174757, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24651721, Length=206, Percent_Identity=34.4660194174757, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI17864154, Length=206, Percent_Identity=34.4660194174757, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI17137380, Length=442, Percent_Identity=26.4705882352941, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19921738, Length=192, Percent_Identity=30.7291666666667, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281363316, Length=178, Percent_Identity=28.6516853932584, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI17864358, Length=178, Percent_Identity=28.6516853932584, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002891
- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF01583 APS_kinase; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4; =2.7.1.25 [H]

Molecular weight: Translated: 45707; Mature: 45576

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00018 EF_HAND_1 ; PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLLRIATAGSVDDGKSTLVGRLLYDTKSVLIDQFDAVTKASESRGLDTPDLSLLVDGL
CCCHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
RAEREQGITIDVAYRYFATPKRSFILADTPGHVQYTRNTVSGASTAQLVILLVDARKGVI
HHHHHCCCEEEEEEEEECCCCCCEEEECCCCCEEEECCCCCCCCCEEEEEEEEECCCCHH
EQTRRHAAVLALLGVPRLVLAVNKIDLVPDSARIFEAISAEFNELTSSLGWKPENVVEIP
HHHHHHHHHHHHHCCCHHHHEEHHEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECC
VSALHGDNIATRSERTGFYNGPTLIEHLESVPAHGESDGASGTIGLRFPVQYVIRPRTAE
HHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEECCEEEEECCCCCC
YPDYRGYAGQVAAGAVRVGDEVVISPAGQRTTVAGIDTADGPLEVAEAGRSITLILADDV
CCCCCCCCCCHHHCEEEECCEEEEECCCCCEEEEECCCCCCCHHHHHCCCEEEEEEECCC
DASRGDLIASLDVPDPVSELDGTVCWLADKPLKAGARLLLKHGTRTVPAIIGALVERFDE
CCCCCCEEEECCCCCCHHHCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QNLSADPAPESLELNDIGQISIRLAEAISVDEYVDDRTAGSFLLIDPSSGNTLAAGLVGN
CCCCCCCCCCCEECCCCCEEEEEHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEHHHHC
ALRAVELNRA
CEEEEEECCC
>Mature Secondary Structure 
SDLLRIATAGSVDDGKSTLVGRLLYDTKSVLIDQFDAVTKASESRGLDTPDLSLLVDGL
CCHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
RAEREQGITIDVAYRYFATPKRSFILADTPGHVQYTRNTVSGASTAQLVILLVDARKGVI
HHHHHCCCEEEEEEEEECCCCCCEEEECCCCCEEEECCCCCCCCCEEEEEEEEECCCCHH
EQTRRHAAVLALLGVPRLVLAVNKIDLVPDSARIFEAISAEFNELTSSLGWKPENVVEIP
HHHHHHHHHHHHHCCCHHHHEEHHEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECC
VSALHGDNIATRSERTGFYNGPTLIEHLESVPAHGESDGASGTIGLRFPVQYVIRPRTAE
HHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEECCEEEEECCCCCC
YPDYRGYAGQVAAGAVRVGDEVVISPAGQRTTVAGIDTADGPLEVAEAGRSITLILADDV
CCCCCCCCCCHHHCEEEECCEEEEECCCCCEEEEECCCCCCCHHHHHCCCEEEEEEECCC
DASRGDLIASLDVPDPVSELDGTVCWLADKPLKAGARLLLKHGTRTVPAIIGALVERFDE
CCCCCCEEEECCCCCCHHHCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QNLSADPAPESLELNDIGQISIRLAEAISVDEYVDDRTAGSFLLIDPSSGNTLAAGLVGN
CCCCCCCCCCCEECCCCCEEEEEHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEHHHHC
ALRAVELNRA
CEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]