Click here to switch to the map view.

The map label for this gene is gatA [H]

Identifier: 169628648

GI number: 169628648

Start: 1584652

End: 1586292

Strand: Reverse

Name: gatA [H]

Synonym: MAB_1558c

Alternate gene names: 169628648

Gene position: 1586292-1584652 (Counterclockwise)

Preceding gene: 169628654

Following gene: 169628643

Centisome position: 31.31

GC content: 68.01

Gene sequence:

>1641_bases
ATGGGTCAGGGCACCCGTACCTTGCTTGACATGACGGTTTGGATCATGCGCCGCCCCGAGGATGACATCGCTGCCGAATT
GGAGGCCTCATCAGGGCCGTTGGCCGGTGTACGGCTGGCGGTCAAGGACAATGTGGACGTGGGCGGCGTGCCGACTACCG
CCGCCTGCCCCGAGTACGCATACGTACCCGATCAGGATGCGCCGGCCGTCGCGGCGCTGCGTGCGGCAGGCGCCGTGGTG
GTGGGCAAGACCAACCTGGACCAGTTCGCGACCGGGCTGGTGGGCACGCGCTCCCCGTATGGCGCGGTATCGGACTCCCG
CCGTCCCGAGTACATCAGCGGCGGTTCCAGTTCGGGATCCGCCGTGGCCGTCGCCACCGGACAGGCCGATATCGCGATCG
GCACCGACACCGCCGGTTCCGGCCGCGTGCCGGCGGGGCTGCAGGGCATTGTCGGCATCAAACCAACAGTCGGCGTCATC
TCGACCGAAGGCGTGGTTCCGGCATGCGAATCCTACGATTGTGTAACCATTTTCGCCCCCTCCCTAACCACCGCCAACGC
GGCGATGGCCGCGATGGGCGCGGCGGCGGGGCCCCGGCTTTGGCCCGCCAACACCCGGCTGGCCGCTCCGCCGCAACCCA
CGATCGCCGTCCCGCGCGAGCTGCCCGCCCTGGACGAGGTGTGGTGTAACGCGTTTCGCGGAGCCGTCGACGATCTGCGC
GCCGCCGGCGCGACCATCATCGAGATCGATCTCACCCCGTTCCTGGCTGCCGCGAAGCTGCTGTACGAGGGCGCGCTGGT
CAGCGAGCGTTATGCCGCGGTGGGCGAGTTCATCGACGCCCACCCCGAGGCATCGCTGGATCCCACTGTTGCACAGATCA
TTTCCGGTGCACGCGATATCCCGGCGCATCGCCTGGTCCACGACCGCGCCGAGGTGCAGCGCCTACGCGAGCAGGCCATG
ACCTCGCTGGCGGGTGCGGATGCATTACTGGTGCCGACAGCACCGCTACATCCCACGATCGAGCAGGTGAATGCCAACCC
CATCGGCGTCAACGCGACCATGGGGACCTACACCAACTTCTGCAACCTGTTCGATCTGTGCGCGGTGTCGGTGCCCGCCG
GAACCGCGGGCGACGCCCAGTTTGGCGTGACGGTTATCGCGCGGGCATTCGATGATGCGGTCGCCTTCGACATCGCCGCG
CTCGCTACCGGAGCACCTGCGGGACAAGATGTTTGGCCTGCCGCGATCACGCTGTCCCATGAACTTGCGGTGTTCGGCGC
ACATCTCAAAGGCGGCCCGCTGGAGTTTCAACTCACCGACCTGGGCGCACGGTGGGTCGGCCCGATCCGCACCGCGACGA
AGTACCGGATGTCGGCGCTGCGCACCACGCCACCCAAACCGGGCCTTACCCGCTCCGAGGAGAACGGCGTGAGCATCGGC
GGCGAGGTCTGGCGCCTCTCCCCCGCCGCGCTGGGCACCTTCCTCGCGCAGCTGCCTGAACCGATGCTGCTGGGCAAGGT
CGAATGTGATGACGGCTTGTGGCGCACCGGCTTTGGATGCGACGGTGCCGCCGCACAGGCCGGCGTCGACATCAGCGAGC
ACGGCAGCTGGCCAGCAGCCATCGCGGCCGGAGCGGTCTAA

Upstream 100 bases:

>100_bases
GGTGACTCCAGTAACCCCGGCGACACCGACACCCGCCGACAGACGTGGGCACGTCCGGACGGTGGTTGTGGATGAACATT
GGATTGGGGATAAGTGGCCG

Downstream 100 bases:

>100_bases
CCGCGCGTTTTCTCGTTGTGCTGGGCCATTTTCGCGAGCATGGCGTTGTAGGCGGCCAGGTCCGCGTCGTCATCGCGGTC
GGCGGCGCGATCCTGGCGGC

Product: allophanate hydrolase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MGQGTRTLLDMTVWIMRRPEDDIAAELEASSGPLAGVRLAVKDNVDVGGVPTTAACPEYAYVPDQDAPAVAALRAAGAVV
VGKTNLDQFATGLVGTRSPYGAVSDSRRPEYISGGSSSGSAVAVATGQADIAIGTDTAGSGRVPAGLQGIVGIKPTVGVI
STEGVVPACESYDCVTIFAPSLTTANAAMAAMGAAAGPRLWPANTRLAAPPQPTIAVPRELPALDEVWCNAFRGAVDDLR
AAGATIIEIDLTPFLAAAKLLYEGALVSERYAAVGEFIDAHPEASLDPTVAQIISGARDIPAHRLVHDRAEVQRLREQAM
TSLAGADALLVPTAPLHPTIEQVNANPIGVNATMGTYTNFCNLFDLCAVSVPAGTAGDAQFGVTVIARAFDDAVAFDIAA
LATGAPAGQDVWPAAITLSHELAVFGAHLKGGPLEFQLTDLGARWVGPIRTATKYRMSALRTTPPKPGLTRSEENGVSIG
GEVWRLSPAALGTFLAQLPEPMLLGKVECDDGLWRTGFGCDGAAAQAGVDISEHGSWPAAIAAGAV

Sequences:

>Translated_546_residues
MGQGTRTLLDMTVWIMRRPEDDIAAELEASSGPLAGVRLAVKDNVDVGGVPTTAACPEYAYVPDQDAPAVAALRAAGAVV
VGKTNLDQFATGLVGTRSPYGAVSDSRRPEYISGGSSSGSAVAVATGQADIAIGTDTAGSGRVPAGLQGIVGIKPTVGVI
STEGVVPACESYDCVTIFAPSLTTANAAMAAMGAAAGPRLWPANTRLAAPPQPTIAVPRELPALDEVWCNAFRGAVDDLR
AAGATIIEIDLTPFLAAAKLLYEGALVSERYAAVGEFIDAHPEASLDPTVAQIISGARDIPAHRLVHDRAEVQRLREQAM
TSLAGADALLVPTAPLHPTIEQVNANPIGVNATMGTYTNFCNLFDLCAVSVPAGTAGDAQFGVTVIARAFDDAVAFDIAA
LATGAPAGQDVWPAAITLSHELAVFGAHLKGGPLEFQLTDLGARWVGPIRTATKYRMSALRTTPPKPGLTRSEENGVSIG
GEVWRLSPAALGTFLAQLPEPMLLGKVECDDGLWRTGFGCDGAAAQAGVDISEHGSWPAAIAAGAV
>Mature_545_residues
GQGTRTLLDMTVWIMRRPEDDIAAELEASSGPLAGVRLAVKDNVDVGGVPTTAACPEYAYVPDQDAPAVAALRAAGAVVV
GKTNLDQFATGLVGTRSPYGAVSDSRRPEYISGGSSSGSAVAVATGQADIAIGTDTAGSGRVPAGLQGIVGIKPTVGVIS
TEGVVPACESYDCVTIFAPSLTTANAAMAAMGAAAGPRLWPANTRLAAPPQPTIAVPRELPALDEVWCNAFRGAVDDLRA
AGATIIEIDLTPFLAAAKLLYEGALVSERYAAVGEFIDAHPEASLDPTVAQIISGARDIPAHRLVHDRAEVQRLREQAMT
SLAGADALLVPTAPLHPTIEQVNANPIGVNATMGTYTNFCNLFDLCAVSVPAGTAGDAQFGVTVIARAFDDAVAFDIAAL
ATGAPAGQDVWPAAITLSHELAVFGAHLKGGPLEFQLTDLGARWVGPIRTATKYRMSALRTTPPKPGLTRSEENGVSIGG
EVWRLSPAALGTFLAQLPEPMLLGKVECDDGLWRTGFGCDGAAAQAGVDISEHGSWPAAIAAGAV

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=432, Percent_Identity=28.7037037037037, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI195972892, Length=187, Percent_Identity=29.9465240641711, Blast_Score=77, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=408, Percent_Identity=27.9411764705882, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17537465, Length=155, Percent_Identity=32.9032258064516, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=541, Percent_Identity=43.6229205175601, Blast_Score=369, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6323950, Length=163, Percent_Identity=36.8098159509202, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648113, Length=251, Percent_Identity=36.2549800796813, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 56026; Mature: 55895

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQGTRTLLDMTVWIMRRPEDDIAAELEASSGPLAGVRLAVKDNVDVGGVPTTAACPEYA
CCCCHHHHHHHHHHHHCCCCHHHHHEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCC
YVPDQDAPAVAALRAAGAVVVGKTNLDQFATGLVGTRSPYGAVSDSRRPEYISGGSSSGS
CCCCCCCCHHHHHHHCCEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCC
AVAVATGQADIAIGTDTAGSGRVPAGLQGIVGIKPTVGVISTEGVVPACESYDCVTIFAP
EEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEC
SLTTANAAMAAMGAAAGPRLWPANTRLAAPPQPTIAVPRELPALDEVWCNAFRGAVDDLR
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHH
AAGATIIEIDLTPFLAAAKLLYEGALVSERYAAVGEFIDAHPEASLDPTVAQIISGARDI
HCCCEEEEEECCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCC
PAHRLVHDRAEVQRLREQAMTSLAGADALLVPTAPLHPTIEQVNANPIGVNATMGTYTNF
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHCCCCCCEEECCHHHHHHH
CNLFDLCAVSVPAGTAGDAQFGVTVIARAFDDAVAFDIAALATGAPAGQDVWPAAITLSH
HHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEC
ELAVFGAHLKGGPLEFQLTDLGARWVGPIRTATKYRMSALRTTPPKPGLTRSEENGVSIG
EEEEEEEECCCCCEEEEEECCCCHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEC
GEVWRLSPAALGTFLAQLPEPMLLGKVECDDGLWRTGFGCDGAAAQAGVDISEHGSWPAA
CEEEEECHHHHHHHHHHCCCCCEEEEEECCCCCEECCCCCCCCHHHCCCCHHHCCCCCHH
IAAGAV
HHCCCC
>Mature Secondary Structure 
GQGTRTLLDMTVWIMRRPEDDIAAELEASSGPLAGVRLAVKDNVDVGGVPTTAACPEYA
CCCHHHHHHHHHHHHCCCCHHHHHEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCC
YVPDQDAPAVAALRAAGAVVVGKTNLDQFATGLVGTRSPYGAVSDSRRPEYISGGSSSGS
CCCCCCCCHHHHHHHCCEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCC
AVAVATGQADIAIGTDTAGSGRVPAGLQGIVGIKPTVGVISTEGVVPACESYDCVTIFAP
EEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEC
SLTTANAAMAAMGAAAGPRLWPANTRLAAPPQPTIAVPRELPALDEVWCNAFRGAVDDLR
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHH
AAGATIIEIDLTPFLAAAKLLYEGALVSERYAAVGEFIDAHPEASLDPTVAQIISGARDI
HCCCEEEEEECCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCC
PAHRLVHDRAEVQRLREQAMTSLAGADALLVPTAPLHPTIEQVNANPIGVNATMGTYTNF
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHCCCCCCEEECCHHHHHHH
CNLFDLCAVSVPAGTAGDAQFGVTVIARAFDDAVAFDIAALATGAPAGQDVWPAAITLSH
HHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEC
ELAVFGAHLKGGPLEFQLTDLGARWVGPIRTATKYRMSALRTTPPKPGLTRSEENGVSIG
EEEEEEEECCCCCEEEEEECCCCHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEC
GEVWRLSPAALGTFLAQLPEPMLLGKVECDDGLWRTGFGCDGAAAQAGVDISEHGSWPAA
CEEEEECHHHHHHHHHHCCCCCEEEEEECCCCCEECCCCCCCCHHHCCCCHHHCCCCCHH
IAAGAV
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA