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The map label for this gene is pncA [H]

Identifier: 169628563

GI number: 169628563

Start: 1486415

End: 1486969

Strand: Reverse

Name: pncA [H]

Synonym: MAB_1472c

Alternate gene names: 169628563

Gene position: 1486969-1486415 (Counterclockwise)

Preceding gene: 169628564

Following gene: 169628562

Centisome position: 29.35

GC content: 67.03

Gene sequence:

>555_bases
ATGGGCAGGGCGCTTATCGTCGTCGACGTGCAGAACGACTTCTGCGAGGGAGGCTCGCTGTCCGTGCCCGGCGGCGCCGC
CCTGGCCCGGACACTGAACCACCTCACCCGCTCGGGCGCCTATGACGCGGTGGTGGCGACCCGGGACTTTCACGTCGACC
CCGGCGGCCACTTTTCGGAGAATCCGGACTTCCAGACGAGCTGGCCACCACATTGCCGGGCCGGCACACCCGGCGCGGAC
TTTCATCCCTATCTGGATATGGGCCCCGTCGACGAGGTGTTTTCCAAAGGTGCGTACAGCGCGGCATACTCGGGATTCGA
GGGCGTCGCCACCGACGGCACCGCACTGGCAGCCTGGCTGCACAGCCGGGAACTGAATGACGTCGACGTGGTGGGCATCG
CCACCGACTACTGCGTTGCCGCGACCGCCCGCGACGCGGCGCACGAGGGACTGCATACTCGCGTGCTTACCGCCTATTCC
GTTGGCATCTCTGAGGAATCGGTGAATCGCGCCCTGGACGAGCTCCAGCGCGCCGGCGTCGAGGTGATCCGCTGA

Upstream 100 bases:

>100_bases
CTGGCTATCAGCCGCGACGAACTGATCGACTACATATCGCGCATGGGCCGTCAGGTGTCGGCAATCTTCAACTGAGCGCC
GGCCGGGGTACCGTCGATCC

Downstream 100 bases:

>100_bases
AGGAGAAATTGAGCGACACATCCACAACATCACATGGTCTTTCCGCGAGCGGCTCATCCACAGGCCTGCTAGCCACCGCC
GTCGCGGCCCTCGGCGGCAC

Product: putative nicotinamidase/pyrazinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MGRALIVVDVQNDFCEGGSLSVPGGAALARTLNHLTRSGAYDAVVATRDFHVDPGGHFSENPDFQTSWPPHCRAGTPGAD
FHPYLDMGPVDEVFSKGAYSAAYSGFEGVATDGTALAAWLHSRELNDVDVVGIATDYCVAATARDAAHEGLHTRVLTAYS
VGISEESVNRALDELQRAGVEVIR

Sequences:

>Translated_184_residues
MGRALIVVDVQNDFCEGGSLSVPGGAALARTLNHLTRSGAYDAVVATRDFHVDPGGHFSENPDFQTSWPPHCRAGTPGAD
FHPYLDMGPVDEVFSKGAYSAAYSGFEGVATDGTALAAWLHSRELNDVDVVGIATDYCVAATARDAAHEGLHTRVLTAYS
VGISEESVNRALDELQRAGVEVIR
>Mature_183_residues
GRALIVVDVQNDFCEGGSLSVPGGAALARTLNHLTRSGAYDAVVATRDFHVDPGGHFSENPDFQTSWPPHCRAGTPGADF
HPYLDMGPVDEVFSKGAYSAAYSGFEGVATDGTALAAWLHSRELNDVDVVGIATDYCVAATARDAAHEGLHTRVLTAYSV
GISEESVNRALDELQRAGVEVIR

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=199, Percent_Identity=34.6733668341709, Blast_Score=100, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71999339, Length=220, Percent_Identity=29.0909090909091, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321401, Length=219, Percent_Identity=29.2237442922374, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 19490; Mature: 19358

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRALIVVDVQNDFCEGGSLSVPGGAALARTLNHLTRSGAYDAVVATRDFHVDPGGHFSE
CCCEEEEEECCCCCCCCCCEECCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCC
NPDFQTSWPPHCRAGTPGADFHPYLDMGPVDEVFSKGAYSAAYSGFEGVATDGTALAAWL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHH
HSRELNDVDVVGIATDYCVAATARDAAHEGLHTRVLTAYSVGISEESVNRALDELQRAGV
HHCCCCCEEEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EVIR
HHCC
>Mature Secondary Structure 
GRALIVVDVQNDFCEGGSLSVPGGAALARTLNHLTRSGAYDAVVATRDFHVDPGGHFSE
CCEEEEEECCCCCCCCCCEECCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCC
NPDFQTSWPPHCRAGTPGADFHPYLDMGPVDEVFSKGAYSAAYSGFEGVATDGTALAAWL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHH
HSRELNDVDVVGIATDYCVAATARDAAHEGLHTRVLTAYSVGISEESVNRALDELQRAGV
HHCCCCCEEEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EVIR
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]