Click here to switch to the map view.

The map label for this gene is prfA

Identifier: 169628533

GI number: 169628533

Start: 1447237

End: 1448307

Strand: Direct

Name: prfA

Synonym: MAB_1442

Alternate gene names: 169628533

Gene position: 1447237-1448307 (Clockwise)

Preceding gene: 169628532

Following gene: 169628534

Centisome position: 28.56

GC content: 65.36

Gene sequence:

>1071_bases
ATGAGCGAGACTCCGCTGATCGACGCGATGCTGGCCGAGCACGCTGAGCTGGAGAAGCAACTGGCCGATCCGGCGCTGCA
CGCCGACGCCGCCGCGGCGCGCAAGGCCGGTCGCCGGTTCGCGATGCTGTCGCCGATCGTCGCCACCCACCGAAAGCTTG
CCACCGCCCGCGACGACCTGGCTACGGCCCGTGAGCTGTCGGCGGACGATCCCTCGTTCGCCGATGAGGTGACGGAGCTG
GAATCGTCGATCGCCGAGCTGGAAACGCAACTTTCGGACATGCTGGCACCGCGTGATCCTCACGATGGTGACGACATCCT
GCTGGAAGTGAAATCAGGCGAGGGCGGCGAGGAATCGGCGCTATTCGCCGCCGATCTGGCTCGCATGTACATCCGCTACG
CCGAGCGGCATGGCTGGAAGGTGACGGTTCTCGATGAGACCGAGTCCGACCTCGGCGGGTACAAGGACGCCACCCTGGCG
ATCGCGAGCAAGGGCGACAGCGCCGATGGTGTCTGGTCACGGTTGAAATTTGAAGGCGGCGTACACCGCGTTCAGCGTGT
GCCGGTCACTGAATCTCAAGGGCGCGTGCACACCTCGGCGGCCGGCGTGCTGGTGTACCCCGAGCCTGAAGAGGTCGAGG
AAATCCAGATCGACGAATCGGACCTGCGCATCGATGTGTACCGCTCCTCGGGCAAGGGCGGCCAGGGCGTCAACACCACC
GATTCCGCGGTGCGCATCACGCATCTGCCCACCGGGATCGTCGTGACGTGTCAGAACGAGCGCTCGCAGCTGCAGAACAA
GGCGCGCGCTATGCAGGTATTGGCCGCGAGGCTGCAGGCACTTGCCGAGGAGCAGGCGCAGGCAGATGCCTCGGCCGGTC
GTGCCAGCCAAATCCGCACCGTGGATCGCAGCGAGCGGATCCGCACCTATAACTTCCCGGAGAACCGCATCACCGATCAC
CGCGTCGGGTTCAAGGCGCATAACCTGGACCAGGTTCTCGATGGAGATCTGGATGCCCTGTTCGATGCGTTGGCAGCGGC
CGACCGGAAGGCGCGCCTGCAGGAGGCGTGA

Upstream 100 bases:

>100_bases
AAATAGCTTCCTTGCCGACGCCCGGATCTGCCGTTTGTGCAGTCCGGGCGTCGGTTTTTTTGTATGACCACTGCGCGTAG
GAATGTTGGGAGGAGGGTGC

Downstream 100 bases:

>100_bases
TGAGCCGCTTGCGTGAGCTGCTCGCCGAGGCAGCCGAATCTCTTTCACGGGCAGGTGTTTCCAGCCCTCAGGTGGACGCC
GAGGAGCTGGCCGCCCACCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MSETPLIDAMLAEHAELEKQLADPALHADAAAARKAGRRFAMLSPIVATHRKLATARDDLATARELSADDPSFADEVTEL
ESSIAELETQLSDMLAPRDPHDGDDILLEVKSGEGGEESALFAADLARMYIRYAERHGWKVTVLDETESDLGGYKDATLA
IASKGDSADGVWSRLKFEGGVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEVEEIQIDESDLRIDVYRSSGKGGQGVNTT
DSAVRITHLPTGIVVTCQNERSQLQNKARAMQVLAARLQALAEEQAQADASAGRASQIRTVDRSERIRTYNFPENRITDH
RVGFKAHNLDQVLDGDLDALFDALAAADRKARLQEA

Sequences:

>Translated_356_residues
MSETPLIDAMLAEHAELEKQLADPALHADAAAARKAGRRFAMLSPIVATHRKLATARDDLATARELSADDPSFADEVTEL
ESSIAELETQLSDMLAPRDPHDGDDILLEVKSGEGGEESALFAADLARMYIRYAERHGWKVTVLDETESDLGGYKDATLA
IASKGDSADGVWSRLKFEGGVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEVEEIQIDESDLRIDVYRSSGKGGQGVNTT
DSAVRITHLPTGIVVTCQNERSQLQNKARAMQVLAARLQALAEEQAQADASAGRASQIRTVDRSERIRTYNFPENRITDH
RVGFKAHNLDQVLDGDLDALFDALAAADRKARLQEA
>Mature_355_residues
SETPLIDAMLAEHAELEKQLADPALHADAAAARKAGRRFAMLSPIVATHRKLATARDDLATARELSADDPSFADEVTELE
SSIAELETQLSDMLAPRDPHDGDDILLEVKSGEGGEESALFAADLARMYIRYAERHGWKVTVLDETESDLGGYKDATLAI
ASKGDSADGVWSRLKFEGGVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEVEEIQIDESDLRIDVYRSSGKGGQGVNTTD
SAVRITHLPTGIVVTCQNERSQLQNKARAMQVLAARLQALAEEQAQADASAGRASQIRTVDRSERIRTYNFPENRITDHR
VGFKAHNLDQVLDGDLDALFDALAAADRKARLQEA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=272, Percent_Identity=47.0588235294118, Blast_Score=245, Evalue=5e-65,
Organism=Homo sapiens, GI34577120, Length=335, Percent_Identity=37.0149253731343, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI166795305, Length=177, Percent_Identity=47.4576271186441, Blast_Score=160, Evalue=1e-39,
Organism=Escherichia coli, GI1787462, Length=344, Percent_Identity=47.9651162790698, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI2367172, Length=334, Percent_Identity=38.0239520958084, Blast_Score=206, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17542784, Length=318, Percent_Identity=36.4779874213836, Blast_Score=171, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6321295, Length=357, Percent_Identity=39.4957983193277, Blast_Score=239, Evalue=3e-64,
Organism=Drosophila melanogaster, GI19921226, Length=340, Percent_Identity=37.3529411764706, Blast_Score=218, Evalue=4e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_MYCA9 (B1MLV1)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001702182.1
- ProteinModelPortal:   B1MLV1
- SMR:   B1MLV1
- EnsemblBacteria:   EBMYCT00000006238
- GeneID:   5967442
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_1442
- GeneTree:   EBGT00050000015865
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   MABS561007:MAB_1442-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 38804; Mature: 38673

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETPLIDAMLAEHAELEKQLADPALHADAAAARKAGRRFAMLSPIVATHRKLATARDDL
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ATARELSADDPSFADEVTELESSIAELETQLSDMLAPRDPHDGDDILLEVKSGEGGEESA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHH
LFAADLARMYIRYAERHGWKVTVLDETESDLGGYKDATLAIASKGDSADGVWSRLKFEGG
HHHHHHHHHHHHHHHHCCCEEEEECCCCHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCC
VHRVQRVPVTESQGRVHTSAAGVLVYPEPEEVEEIQIDESDLRIDVYRSSGKGGQGVNTT
HHHHHCCCCCCCCCCEEECCCEEEECCCCCCHHHEEECCCCCEEEEEECCCCCCCCCCCC
DSAVRITHLPTGIVVTCQNERSQLQNKARAMQVLAARLQALAEEQAQADASAGRASQIRT
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VDRSERIRTYNFPENRITDHRVGFKAHNLDQVLDGDLDALFDALAAADRKARLQEA
CCHHCCCEECCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SETPLIDAMLAEHAELEKQLADPALHADAAAARKAGRRFAMLSPIVATHRKLATARDDL
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ATARELSADDPSFADEVTELESSIAELETQLSDMLAPRDPHDGDDILLEVKSGEGGEESA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHH
LFAADLARMYIRYAERHGWKVTVLDETESDLGGYKDATLAIASKGDSADGVWSRLKFEGG
HHHHHHHHHHHHHHHHCCCEEEEECCCCHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCC
VHRVQRVPVTESQGRVHTSAAGVLVYPEPEEVEEIQIDESDLRIDVYRSSGKGGQGVNTT
HHHHHCCCCCCCCCCEEECCCEEEECCCCCCHHHEEECCCCCEEEEEECCCCCCCCCCCC
DSAVRITHLPTGIVVTCQNERSQLQNKARAMQVLAARLQALAEEQAQADASAGRASQIRT
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VDRSERIRTYNFPENRITDHRVGFKAHNLDQVLDGDLDALFDALAAADRKARLQEA
CCHHCCCEECCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA