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The map label for this gene is ubiE [H]

Identifier: 169628481

GI number: 169628481

Start: 1386372

End: 1387013

Strand: Reverse

Name: ubiE [H]

Synonym: MAB_1390c

Alternate gene names: 169628481

Gene position: 1387013-1386372 (Counterclockwise)

Preceding gene: 169628483

Following gene: 169628480

Centisome position: 27.37

GC content: 65.73

Gene sequence:

>642_bases
ATGCCTGCCATCGCCCAGCGGCGTTCGCTCAACGAGTTGGTCACGCGGTACTGGACCCTGATGTCCAGGGTCTACAACTT
CCCACTCGTGCAGCGCCATATCTACAGCCCTGCCCAGACAGACACCATAGCGCAGCTATGCGAGCACGACGCCCGGCGTG
TTGCCGATATCGCTTGCGGCACTGGAATATTGGCATCGCGCATTCGGGCAGAGTTGCACCAGGCAACCGTCTTCGGCGTT
GATATGTCCGACGGCATGCTCGCCAAGGCCAGGGCGCGGTGCGCTCAGGTGCAGTGGCGCAAGGCGCCCGCCGAGCAGCT
CCCCTTCGCGGATTCCAGCCTCGACGCCGTAGTGACGACGTCGGCCTTCCATTTCTTCGACCAGCCTGCCGCGCTGGCAG
AGTTCTACCGCGTGCTGGTCCCCGGCGGGATGGCCTCGGTCGCCACGATCTGCCCCAGGGAGCGGCGCTGGGCACGCAAC
CGCACCGGCGAACGTGGCCCGGCGCATTTCCCGACGGCCACCGAGATGGAACGGTTGTTCGCAGGTGCGGGCTTTGAGGT
GGTCGTGCACCGCCCGGTGGCGCGACCCGTTACACCACCGCTGGTTTCCGTCGACTGGATCTGTGTGGGCCTCAAGACCT
GA

Upstream 100 bases:

>100_bases
ATTGGGACCGGTCATAGCTTCCGGCCGCGCGCCTCGTGTCTTTCACCAAGGTCGGCACAAGTCGCGGACCCGGCGCCGCC
GCCGCTCCTAGATTTCACAG

Downstream 100 bases:

>100_bases
CGGTCACTCGCCCGCCGCCATGATCATGCCGTACGGCCATCCATTACCCTCACTGATTGACGTCAGCCTGACAACGTAAA
TCTTGAGGAGTACCCATGGA

Product: putative UbiE/COQ5 methyltransferase

Products: S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MPAIAQRRSLNELVTRYWTLMSRVYNFPLVQRHIYSPAQTDTIAQLCEHDARRVADIACGTGILASRIRAELHQATVFGV
DMSDGMLAKARARCAQVQWRKAPAEQLPFADSSLDAVVTTSAFHFFDQPAALAEFYRVLVPGGMASVATICPRERRWARN
RTGERGPAHFPTATEMERLFAGAGFEVVVHRPVARPVTPPLVSVDWICVGLKT

Sequences:

>Translated_213_residues
MPAIAQRRSLNELVTRYWTLMSRVYNFPLVQRHIYSPAQTDTIAQLCEHDARRVADIACGTGILASRIRAELHQATVFGV
DMSDGMLAKARARCAQVQWRKAPAEQLPFADSSLDAVVTTSAFHFFDQPAALAEFYRVLVPGGMASVATICPRERRWARN
RTGERGPAHFPTATEMERLFAGAGFEVVVHRPVARPVTPPLVSVDWICVGLKT
>Mature_212_residues
PAIAQRRSLNELVTRYWTLMSRVYNFPLVQRHIYSPAQTDTIAQLCEHDARRVADIACGTGILASRIRAELHQATVFGVD
MSDGMLAKARARCAQVQWRKAPAEQLPFADSSLDAVVTTSAFHFFDQPAALAEFYRVLVPGGMASVATICPRERRWARNR
TGERGPAHFPTATEMERLFAGAGFEVVVHRPVARPVTPPLVSVDWICVGLKT

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=146, Percent_Identity=32.1917808219178, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.51 [C]

Molecular weight: Translated: 23648; Mature: 23516

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAIAQRRSLNELVTRYWTLMSRVYNFPLVQRHIYSPAQTDTIAQLCEHDARRVADIACG
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
TGILASRIRAELHQATVFGVDMSDGMLAKARARCAQVQWRKAPAEQLPFADSSLDAVVTT
HHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHH
SAFHFFDQPAALAEFYRVLVPGGMASVATICPRERRWARNRTGERGPAHFPTATEMERLF
HHHHHHCCHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHCCCCCCCCCCCCCHHHHHHHH
AGAGFEVVVHRPVARPVTPPLVSVDWICVGLKT
HCCCCEEEEECCCCCCCCCCCEEECEEEEECCC
>Mature Secondary Structure 
PAIAQRRSLNELVTRYWTLMSRVYNFPLVQRHIYSPAQTDTIAQLCEHDARRVADIACG
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
TGILASRIRAELHQATVFGVDMSDGMLAKARARCAQVQWRKAPAEQLPFADSSLDAVVTT
HHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHH
SAFHFFDQPAALAEFYRVLVPGGMASVATICPRERRWARNRTGERGPAHFPTATEMERLF
HHHHHHCCHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHCCCCCCCCCCCCCHHHHHHHH
AGAGFEVVVHRPVARPVTPPLVSVDWICVGLKT
HCCCCEEEEECCCCCCCCCCCEEECEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Unidentified protein factor B [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; rRNA [C]

Specific reaction: S-Adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

General reaction: Methyl group transfer [C]

Inhibitor: Mg2+; Unidentified protein factor A1 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA