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The map label for this gene is yfnC [H]

Identifier: 169628431

GI number: 169628431

Start: 1339727

End: 1340926

Strand: Direct

Name: yfnC [H]

Synonym: MAB_1338

Alternate gene names: 169628431

Gene position: 1339727-1340926 (Clockwise)

Preceding gene: 169628429

Following gene: 169628432

Centisome position: 26.44

GC content: 67.58

Gene sequence:

>1200_bases
GTGACCGATTTGGCGAACGATGAACAGCTGACCGTGCGGCATGACCGGCGGATTACGCCAACACCCGTGTCGGCGTGGAA
TCGCATGCGGTACTGGGTGATCGCGCATGCTGTCGACGACTTCTACCAGGGCCTGGTGCCCGCGGCCATCCCGTTCTTCG
TGCTAGAGCGTCACTACAGCTATGCGGCAGCATCGGGTCTGGCGCTCGCCGCGACGCTGGGAAGCGCGCTGCCCCAGCCG
CTGCTCGGTCTCATCGCCGATCGCTGGCGACTGCTGTGGATGGCGCCGGCGGGCCTGGCCGCGGCCGGAATAGGCGCCGG
CCTCGCCGGACTGGTACCCGGCTACTGGTTGGTCTGGACGATGCTGCTCGTCTCCGGACTGGGGGTGGCGGCCTTCCATC
CCGCTGCGGGGCGCGACGCCCGCCGCGACGCGGGTGACAGCGCCACCGCGATGAGCCTGTTCGCCGCGGGCGGCAGCGTC
GGTTTCTTTCTGGCGCCGGCGCTGGTGACACCCGCGCTGGTGTCTGCGGGCCTGGGCGCCACGGCCTGGTTCATCCCGCC
CGCCGTCATCATGGGATTCGTACTCTGGCGCTATCAACTTCGCCAGGGTTCGGCGAATTCGGTGGACCGGCAGCTGACGG
GCAATGATCGTTGGGGCGCATTCTCGATTTTGGTCGCCGTAGAGGTGGTGCGCTCGGCCGTCTACTTCGGCATGAACACC
TTCATCGCGCTATACCTGATCGATCACCTTGATGTTTCCGCGGCGGTGGGCGGTGTGGGGCTGGCGATGTTCCTGGGCGG
CGGTGTGATCGGCACACTGCTGGGCGGCCGCATCAGCGACCGCCTCGGCATGATCGCCACCATCCGAGTGGGCAGTATCG
TCGCGATCCCCGCGTTCGTCGCGCTGCGGATATGCCCGCAGCCGTGGATCGCGCTGGCGCTGGCCGCCGTCGTCGGCATA
ACCGTCAACATCCCGTTCGCCGTGCTGGTGAAACTGGGCCAGGACTATCTGCCGACACGTCCCGGCACAGCCGCGGGCGT
CACGCTGGGCCTAGCGGTCAGCATCGGCGGATTCTTCGTTCCGCTGCTGGGCGTACTTGCCGATCACCACGGGCCGCAGG
CGGTGCTGACGACCCTTTGCGTCGCGCCGATTCTGGCGATAGTGCTGGCCTTCTTGCTGCCGCACCCGGAGCGGGAATAG

Upstream 100 bases:

>100_bases
ACCGGCCACCGACATCACCCCGGTGAGGTGGCGGTTAATCAACACAAGATGGCATTTTATCGGAGGCTTACGGCTCGGCA
AACCCATAATCTCGTCCCTC

Downstream 100 bases:

>100_bases
CGAACCGACGGCGGCGGGTTGGCCGAGACGTGAAACTTGGCGTGGCCATCGGCCGACCCCTCAGCGTGCAGAACTCCACA
AACCTCGTCGACACATTCAT

Product: putative membrane efflux protein

Products: fosmidomycin [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTDLANDEQLTVRHDRRITPTPVSAWNRMRYWVIAHAVDDFYQGLVPAAIPFFVLERHYSYAAASGLALAATLGSALPQP
LLGLIADRWRLLWMAPAGLAAAGIGAGLAGLVPGYWLVWTMLLVSGLGVAAFHPAAGRDARRDAGDSATAMSLFAAGGSV
GFFLAPALVTPALVSAGLGATAWFIPPAVIMGFVLWRYQLRQGSANSVDRQLTGNDRWGAFSILVAVEVVRSAVYFGMNT
FIALYLIDHLDVSAAVGGVGLAMFLGGGVIGTLLGGRISDRLGMIATIRVGSIVAIPAFVALRICPQPWIALALAAVVGI
TVNIPFAVLVKLGQDYLPTRPGTAAGVTLGLAVSIGGFFVPLLGVLADHHGPQAVLTTLCVAPILAIVLAFLLPHPERE

Sequences:

>Translated_399_residues
MTDLANDEQLTVRHDRRITPTPVSAWNRMRYWVIAHAVDDFYQGLVPAAIPFFVLERHYSYAAASGLALAATLGSALPQP
LLGLIADRWRLLWMAPAGLAAAGIGAGLAGLVPGYWLVWTMLLVSGLGVAAFHPAAGRDARRDAGDSATAMSLFAAGGSV
GFFLAPALVTPALVSAGLGATAWFIPPAVIMGFVLWRYQLRQGSANSVDRQLTGNDRWGAFSILVAVEVVRSAVYFGMNT
FIALYLIDHLDVSAAVGGVGLAMFLGGGVIGTLLGGRISDRLGMIATIRVGSIVAIPAFVALRICPQPWIALALAAVVGI
TVNIPFAVLVKLGQDYLPTRPGTAAGVTLGLAVSIGGFFVPLLGVLADHHGPQAVLTTLCVAPILAIVLAFLLPHPERE
>Mature_398_residues
TDLANDEQLTVRHDRRITPTPVSAWNRMRYWVIAHAVDDFYQGLVPAAIPFFVLERHYSYAAASGLALAATLGSALPQPL
LGLIADRWRLLWMAPAGLAAAGIGAGLAGLVPGYWLVWTMLLVSGLGVAAFHPAAGRDARRDAGDSATAMSLFAAGGSVG
FFLAPALVTPALVSAGLGATAWFIPPAVIMGFVLWRYQLRQGSANSVDRQLTGNDRWGAFSILVAVEVVRSAVYFGMNTF
IALYLIDHLDVSAAVGGVGLAMFLGGGVIGTLLGGRISDRLGMIATIRVGSIVAIPAFVALRICPQPWIALALAAVVGIT
VNIPFAVLVKLGQDYLPTRPGTAAGVTLGLAVSIGGFFVPLLGVLADHHGPQAVLTTLCVAPILAIVLAFLLPHPERE

Specific function: Confers The Resistance Against Fosmidomycin. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786686, Length=380, Percent_Identity=25.7894736842105, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41771; Mature: 41639

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS00962 RIBOSOMAL_S2_1 ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLANDEQLTVRHDRRITPTPVSAWNRMRYWVIAHAVDDFYQGLVPAAIPFFVLERHYS
CCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAAASGLALAATLGSALPQPLLGLIADRWRLLWMAPAGLAAAGIGAGLAGLVPGYWLVWT
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEECCCCHHHHHHHCCHHHHHHHHHHHHH
MLLVSGLGVAAFHPAAGRDARRDAGDSATAMSLFAAGGSVGFFLAPALVTPALVSAGLGA
HHHHHCCCHHEECCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
TAWFIPPAVIMGFVLWRYQLRQGSANSVDRQLTGNDRWGAFSILVAVEVVRSAVYFGMNT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FIALYLIDHLDVSAAVGGVGLAMFLGGGVIGTLLGGRISDRLGMIATIRVGSIVAIPAFV
HHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHCCEEEEECCCHHHHHHHH
ALRICPQPWIALALAAVVGITVNIPFAVLVKLGQDYLPTRPGTAAGVTLGLAVSIGGFFV
HHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
PLLGVLADHHGPQAVLTTLCVAPILAIVLAFLLPHPERE
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TDLANDEQLTVRHDRRITPTPVSAWNRMRYWVIAHAVDDFYQGLVPAAIPFFVLERHYS
CCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAAASGLALAATLGSALPQPLLGLIADRWRLLWMAPAGLAAAGIGAGLAGLVPGYWLVWT
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEECCCCHHHHHHHCCHHHHHHHHHHHHH
MLLVSGLGVAAFHPAAGRDARRDAGDSATAMSLFAAGGSVGFFLAPALVTPALVSAGLGA
HHHHHCCCHHEECCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
TAWFIPPAVIMGFVLWRYQLRQGSANSVDRQLTGNDRWGAFSILVAVEVVRSAVYFGMNT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FIALYLIDHLDVSAAVGGVGLAMFLGGGVIGTLLGGRISDRLGMIATIRVGSIVAIPAFV
HHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHCCEEEEECCCHHHHHHHH
ALRICPQPWIALALAAVVGITVNIPFAVLVKLGQDYLPTRPGTAAGVTLGLAVSIGGFFV
HHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
PLLGVLADHHGPQAVLTTLCVAPILAIVLAFLLPHPERE
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: fosmidomycin [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: fosmidomycin [Cytoplasm] + Proton [Periplasm] = fosmidomycin [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9141694; 9384377 [H]